LOCUS       NZ_CP151199             3720 bp    DNA     circular CON 17-APR-2024
DEFINITION  Klebsiella variicola strain KVTMDU plasmid p12_KVTMDU, complete
            sequence.
ACCESSION   NZ_CP151199
VERSION     NZ_CP151199.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23446385
            Assembly: GCF_038178705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ota,Y.
  TITLE     Emergence of KPC-2-producing Klebsiella variicola from hospital
            sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Ota,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. July-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.3x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038178705.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 11:53:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,118
            CDSs (total)                      :: 5,998
            Genes (coding)                    :: 5,805
            CDSs (with protein)               :: 5,805
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 68 of 193
            Pseudo Genes (incomplete)         :: 125 of 193
            Pseudo Genes (internal stop)      :: 32 of 193
            Pseudo Genes (multiple problems)  :: 29 of 193
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151199.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /strain="KVTMDU"
                     /isolation_source="sewage"
                     /db_xref="taxon:244366"
                     /plasmid="p12_KVTMDU"
                     /geo_loc_name="Japan"
                     /lat_lon="35.7014 N 139.7642 E"
                     /collection_date="2021-06"
                     /collected_by="Tokyo Medical and Dental Hospital"
     gene            complement(39..299)
                     /locus_tag="LQ252_RS30560"
                     /old_locus_tag="LQ252_30565"
     CDS             complement(39..299)
                     /locus_tag="LQ252_RS30560"
                     /old_locus_tag="LQ252_30565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_122652922.1"
                     /translation="MEIEKLIDRYFLALEKKIIAEIYNKQTRKELEKFIFKCDQELMGF
                     EQRFNIICCTEYYEKQKLIFSINYISAEFEQREKLNLTILK"
                     /name="hypothetical protein"
     gene            complement(1513..2637)
                     /locus_tag="LQ252_RS30565"
                     /old_locus_tag="LQ252_30570"
     CDS             complement(1513..2637)
                     /locus_tag="LQ252_RS30565"
                     /old_locus_tag="LQ252_30570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341723212.1"
                     /translation="MDFKIKKMTVKDSREIKNEIELLAKKANAESQNLGVILDRQIKEG
                     LKEINLNVKKETAEQYRKLFGHKLSLNNNDIVSHLLECNTRNSFDKTRSAFRFCIAEKI
                     QELRKESEQARRERNFDLMQKKTIEAYQMFFFFQRDFLSENRVTWNDISYRKIDSKSKK
                     KTMNSVSSIKAIFEDLKQKPDLFDKYSMILAISSLTGCRPAEIQKGISLEVSHQLLNIC
                     ISGAKVGEDRGQEKRILKFNIKEFAQNEQFNLIMSKMKQNNKLTYKCSKQEYDSLRQYL
                     YINHKGFSLYTLRHRAASELKKDGFSEEMIAAFLGHRVTRSLENYGYAKSGKGGPKIAG
                     VEHSNAIKSNTKRYESKSTPGGGNPSRTRKRALK"
                     /name="hypothetical protein"
     gene            complement(3012..3713)
                     /locus_tag="LQ252_RS30570"
                     /old_locus_tag="LQ252_30575"
     CDS             complement(3012..3713)
                     /locus_tag="LQ252_RS30570"
                     /old_locus_tag="LQ252_30575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_148830253.1"
                     /translation="MTISKKNSELRERFKEYASNKNIFDLADLRYEILKIYCDFKLKSD
                     MNEKERKSQDSRRKAHLTALKKRIKREIVSKIVIDLVKYYNIEKTTFHFFSHICTEILE
                     RNVDNRYILNNFSNMILDEKKELRKLSESRNASNKMSLENSYSELVSMSHIKDKLFRND
                     NFKTAYLKCYGCANEEFSRFKVFAFPDNFETLDFLFEEERIKKEEKEISKIMIEQVEEE
                     QKIQPIKKRRL"
                     /name="hypothetical protein"
ORIGIN
        1 gccctcttta ttttgctaat acatatatgc ttaatatatt attttaatat tgtcaaattt
       61 aatttttctc tttgctcaaa ttctgctgaa atataattta tagaaaatat aagtttctgt
      121 ttctcataat attcagtaca gcatattata ttaaatcgct gttcaaaccc cattagctct
      181 tgatcgcact taaatataaa cttttctaat tcttttcttg tttgtttatt ataaatctca
      241 gcaattattt tcttttctaa tgctaaaaaa tatctatcta tcaatttttc tatttccata
      301 aattttcctt ttttttaatt atatttagaa tttaaatagc aaattgcaaa aaaaaatcaa
      361 atcgccaatt tttttcttcg aactctattt ttttattctt tagtattggt tttctatctt
      421 tctacaaaga agcattgctt tttctctata acagataggt attgttttgc tgtggcttcg
      481 ctaagacctt tgtgttcagc cctttacagg gcttcaccca aaggtctccc ctaccgattt
      541 tttgttgtaa tcccctaaaa agtaaggatt atttcaaaaa aatcagtaag ggcgatttgt
      601 gtgattgcaa agtttggact tcaccacaaa gttctagact atcgagctgt gcattgtttc
      661 caacacacgg caccccctca caccctctag atactgttca taatcatttt ttgcgacagt
      721 taaccccatt ttaaacagct ttataactgt tttggctggg ctttctcata gcctaccaat
      781 acaacactgc tcaccaaaac aaccttagaa ctccgcctcc aggttcggca atttgtgaga
      841 tccccttcta ttgccaacaa ccaaccgaaa ccggaccctc ttgattccac agtttcaggc
      901 acgacacaat atgcgcattt ggttgcttaa ggatttgaaa aactagggat ttttgaagag
      961 acttgtaaca agagaggttt gacaattgcg attttataag ttagattttc acttacatat
     1021 cgtaatccag tgcctgatat cacttgatta caagactttc agaagtcccc acttctttaa
     1081 gtccctcaaa accagcttag gctggttttg ttgtatctgg atctgttata accgatctcc
     1141 ttgttctgtc aatacttctt caaaattttt gattacttta aaatcaaaat tctcactcaa
     1201 tgagactcgt tgtgtcagtt ttgaggcaaa aaatgacact tcaaatgcca acggctgaaa
     1261 gttaattgct caaaaaacca ccaacgaaaa gttaccgggg tcccgttccg ggcattacgc
     1321 acgcgtgccg cctgctaata catatattga ttagttaatt acaccgacca cacaagcaac
     1381 cacagcgatc tgtggttctg ctaatacaca tatgcaacca gccggcacag atctctgctg
     1441 gcaagacgcg cgcgcgaggc taacacacat atgaaaggtc agcattgctg accccttgta
     1501 acgctctaaa attcatttta aggctcgttt acgagtacgg gaagggtttc cccctcctgg
     1561 tgttgatttg ctctcatatc gctttgtatt gcttttaatc gcgtttgagt gctcaacacc
     1621 tgcgattttc ggaccaccct tgcccgattt tgcatagcca tagttttcaa gacttctggt
     1681 tactctatgc cccaagaaag cagcgatcat ttcttctgaa aagccgtctt tttttaattc
     1741 gcttgctgct ctgtgtctta atgtataaag tgaaaaacct ttatgattga tatacaaata
     1801 ttgtctcaat gaatcatact cttgctttga gcatttatat gttaatttat tattttgctt
     1861 catttttgac ataattagat tgaactgctc attttgtgca aattccttta tattaaattt
     1921 taatattctt ttttcttgcc ctcgatcttc gcccactttc gccccgctaa tacatatatt
     1981 taatagttgg tggcttactt ccaggctaat ccctttctgg atctcagcag gtctacaacc
     2041 tgttaaactt gaaattgcta aaatcattga atatttatcg aaaagatctg gcttttgctt
     2101 caaatcttca aaaattgctt taatactgga aacactattc attgtctttt ttttgctttt
     2161 cgagtctatt ttccgataag aaatatcatt ccaagtaacc cggttttcac tcaaaaaatc
     2221 gcgttggaag aaaaaaaaca tttgatatgc ttcaatggtt tttttctgca ttaaatcaaa
     2281 atttctttct cttcttgctt gttcagactc tttcctcaat tcttgtattt tttcagcaat
     2341 gcaaaaccta aaagcagatc ttgttttgtc aaaactgttt cttgtgttgc attctaataa
     2401 atgtgaaaca atgtcattgt tgtttaaact taacttgtga ccaaacagtt ttctatattg
     2461 ttctgctgtt tcttttttca catttaaatt aatttctttc aacccttctt tgatctggcg
     2521 atcaagtatc actcccaggt tctggctttc agcatttgct ttttttgcta acagttcaat
     2581 ctcatttttt atttctctgg aatctttcac cgtcattttt tttattttaa aatccatact
     2641 atcacctctt tttttttatg ttttttgtat tcctcttttt gttgagtagg ttgtattttt
     2701 ttctccattc ctaataaatt tatttttcaa aatcgcttcg ctctaatgaa aaaaaattta
     2761 aaagtcattc cgaaaaaaac acaaagggtt tttcgctccc gcgaactttt cctttcaaat
     2821 ttcgctgacg ctcatttttc aagtaaaaga caacccccca ttattgttat aacaaaaata
     2881 acaataatgg caagtcaaaa gaaaaaatag atcgcagatt ttactaggtt tttcatcaac
     2941 ttacacacag atcaatcgct caaaaaacaa aattaaaatt ttatttttct cacgattcgc
     3001 tgcaaaaaaa attataatct tcttttcttt attggctgta ttttttgttc ttcttcaact
     3061 tgttcaatca ttattttaga tatctctttt tcttcttttt ttattctttc ttcttcaaat
     3121 aaaaaatcta atgtctcaaa attgtctgga aaagcaaaaa ctttaaatct tgaaaattcc
     3181 tcatttgcac aaccataaca ttttaaatat gcagttttga aattgtcatt cctaaataat
     3241 ttgtctttaa tatgtgacat agaaacaagt tcggaataag agttttctaa actcattttg
     3301 tttgaagcat ttctgctttc acttaatttc ctcaactctt ttttttcgtc taaaatcatg
     3361 tttgaaaaat tatttagaat gtatctgtta tctacatttc tttctaaaat ctcagtacat
     3421 atatgagaaa aaaaatgaaa agtcgttttt tctatattgt aatacttcac taaatcaata
     3481 actattttcg aaacaatttc tcttttaatt ctttttttta atgctgttaa atgtgctttt
     3541 cttctggagt cttgagattt tctttctttc tcattcatat cgctttttaa tttgaaatca
     3601 caatatattt ttaatatttc atatctcaaa tcagcaagat caaatatgtt tttattactg
     3661 gcgtattctt taaatcgctc tctcaattca ctattttttt ttgatattgt catatattaa
//
