LOCUS       NZ_CP151194             6376 bp    DNA     circular CON 17-APR-2024
DEFINITION  Klebsiella variicola strain KVTMDU plasmid p7_KVTMDU, complete
            sequence.
ACCESSION   NZ_CP151194
VERSION     NZ_CP151194.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23446385
            Assembly: GCF_038178705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ota,Y.
  TITLE     Emergence of KPC-2-producing Klebsiella variicola from hospital
            sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Ota,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. July-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.3x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038178705.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 11:53:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,118
            CDSs (total)                      :: 5,998
            Genes (coding)                    :: 5,805
            CDSs (with protein)               :: 5,805
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 68 of 193
            Pseudo Genes (incomplete)         :: 125 of 193
            Pseudo Genes (internal stop)      :: 32 of 193
            Pseudo Genes (multiple problems)  :: 29 of 193
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151194.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /strain="KVTMDU"
                     /isolation_source="sewage"
                     /db_xref="taxon:244366"
                     /plasmid="p7_KVTMDU"
                     /geo_loc_name="Japan"
                     /lat_lon="35.7014 N 139.7642 E"
                     /collection_date="2021-06"
                     /collected_by="Tokyo Medical and Dental Hospital"
     gene            <960..1283
                     /locus_tag="LQ252_RS30360"
                     /old_locus_tag="LQ252_30365"
                     /pseudo=""
     CDS             <960..1283
                     /locus_tag="LQ252_RS30360"
                     /old_locus_tag="LQ252_30365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            1431..2638
                     /locus_tag="LQ252_RS30365"
                     /old_locus_tag="LQ252_30370"
     CDS             join(1431..1752,1752..2638)
                     /locus_tag="LQ252_RS30365"
                     /old_locus_tag="LQ252_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_087776199.1"
                     /translation="MTGILLGQEVRKRKTPQEKIAIIQQTMEPGMNVSHVARLHGIQPS
                     LLFKWKKQYQEGSLTAVAAGEEVVPASELTAALKQVRELQRLLGKKTMEVEILKEAVEY
                     GQSPKMDSARALVAKGRGIAMVSRTMGVSRAQLSLRINRSADWQDRRCNRRNEEADAEI
                     LSAILNIISDMPSYGYRRVWGILRKQRRTEGQPPVNAKRLYRIMSEHNLLLLHHKPERP
                     KREHKGKIAVAESDMRWCSDGFEFGCDNGEKLRVTFALDCCDREAIDWAASTGGYDSST
                     VQDVMLRSVEKRFGDRLPDTAVQWLTDNGSAYTAHETRRFAKELNLEPCTTAVSSPQSN
                     GMAERFVKTMKEDYIAFMPKPDVRTALRNLAAAFTHYNENHPHSALGYHSPREYRRQRA
                     SLT"
                     /name="IS3 family transposase"
     gene            2768..3382
                     /locus_tag="LQ252_RS30370"
                     /old_locus_tag="LQ252_30375"
     CDS             2768..3382
                     /locus_tag="LQ252_RS30370"
                     /old_locus_tag="LQ252_30375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322115.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="WP_341723211.1"
                     /translation="MSKKLERPPAPTTIHPIWLRLCHWLNALAMLIMVTSGWRIYNASP
                     LFNFQFANELTLGGWLGGALQWHFAGMWLLSVNGVIYLLCNFFSGRFRQKYWPLSPRGF
                     FKDAFAALRGKLAHTDLSHYNMVQKVAYLFVIADSVLLVVSGLVVWKSVQFSLLRELLG
                     GYDIARFIHFYAMSAMVGFVVVHLLMVALVPRTLLAMLRGR"
                     /name="cytochrome b/b6 domain-containing protein"
     gene            3392..4153
                     /locus_tag="LQ252_RS30375"
                     /old_locus_tag="LQ252_30380"
     CDS             3392..4153
                     /locus_tag="LQ252_RS30375"
                     /old_locus_tag="LQ252_30380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="WP_341723209.1"
                     /translation="MSDKNDIIKEATLLINKQLTQEGRRRFLKQGLTLGGIAMLTGCDI
                     SDNATVERSLSNISRFNDRIQAWLFNDIQLAPVYPESMIATNFPFNAFYGEDEAPEING
                     DNYSLELAGLITNKHPWTLAQLHQMAQVSQVTRHICVEGWSAIGKWGGVPFSLFLKVIG
                     ADLRAKYVSFKCADDYYTSIDMATALHPQTLIALTWDGKVLPRKYGYPMKLRIPTKLGY
                     KNPKHIKVIEVTNSYPGGYWEDQGYNWFGGS"
                     /name="molybdopterin-dependent oxidoreductase"
     gene            4191..>4367
                     /locus_tag="LQ252_RS30380"
                     /old_locus_tag="LQ252_30385"
                     /pseudo=""
     CDS             4191..>4367
                     /locus_tag="LQ252_RS30380"
                     /old_locus_tag="LQ252_30385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /name="pentapeptide MXKDX repeat protein"
     gene            complement(<5038..5243)
                     /locus_tag="LQ252_RS30385"
                     /old_locus_tag="LQ252_30390"
                     /pseudo=""
     CDS             complement(<5038..5243)
                     /locus_tag="LQ252_RS30385"
                     /old_locus_tag="LQ252_30390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047389784.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(5302..5571)
                     /locus_tag="LQ252_RS30390"
                     /old_locus_tag="LQ252_30395"
     CDS             complement(5302..5571)
                     /locus_tag="LQ252_RS30390"
                     /old_locus_tag="LQ252_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /protein_id="WP_341723210.1"
                     /translation="MKKVHIALLCSTIIFGMASASAADHMAKDSMAKDSMAKDSMAKDH
                     MAKDHMAKDSMAKDHMAKDSMAKDCMAKDCMAKDEVVKDGMSTK"
                     /name="pentapeptide MXKDX repeat protein"
     gene            <5790..>5966
                     /locus_tag="LQ252_RS30395"
                     /old_locus_tag="LQ252_30400"
                     /pseudo=""
     CDS             <5790..>5966
                     /locus_tag="LQ252_RS30395"
                     /old_locus_tag="LQ252_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(<5990..6070)
                     /locus_tag="LQ252_RS30400"
                     /old_locus_tag="LQ252_30405"
                     /pseudo=""
     CDS             complement(<5990..6070)
                     /locus_tag="LQ252_RS30400"
                     /old_locus_tag="LQ252_30405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     Typing          complement(6165..6337)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="2.01e-77"
     Typing          142..648
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.43"
                     /coverage="99.0"
                     /evalue="1.39e-118"
ORIGIN
        1 gctgacttac gaacggggca gaaatgtcct agagaatgcc aggacgatac ttagcaagga
       61 agaaacaagg ccacggcgaa gccgtttttc cataggctcc gcccccctga caagcatcac
      121 agaatctgac gctcaaatca gtggtggcga aacccgacag gactgtaaag ataccaggcg
      181 tttccccctg atggctccct cttgcgctct cctgttcccg tcatgcggca tccgtgttgt
      241 ggtggaagct ttacctacat caccacgtcc cgttccgttt agacagttcg ctccaagctg
      301 ggctgtgtgc aagaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      361 cttgagtcca acccggaaaa acacgacaaa acgccactgg cagcagccac tggtaacagg
      421 attagcagag cgagtaatgt aggcagtgct acagagttct tgaagtggtg gcctgagtga
      481 ggctacactg gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccacggtt
      541 agaagatctg ccaggatctt cctaccttcg aaaaaccgcc tccccaggcg gttttttcgt
      601 ttacagagca agagattacg acgagataaa aaggatcaca agaagatcct ttctttattg
      661 caaacaacta aaagtgattt cagtaatgcc cacccctgac atgggaacca acataccttc
      721 cctgctcagt ctccatgctg gataactggg tatcagttgt gagtattttt atgtgataaa
      781 agaaaaatag gaacccaaat aattccaatt acggacaaat agcatttgtc cgtatcaaag
      841 gcagacatag ctacttttat gttacttggg tgaatcgcac gggaatccta gagactaaac
      901 tctttgaaaa agagataaac aggatgacta aaaacatacg tttttctcct gaggtccgtc
      961 aggcgctgtg gtcccgtcgc ccgtcaggcg ccattcatca tagcgataag ggttcgtagt
     1021 acgtctggct ggcctacacg cagcggctta aggatgcaga actgctggca tcaacaggca
     1081 gcacaggaga ttcatacgac cacgcgatgg cagagagcat caacgggctt tacaaagcgg
     1141 aggtggaaaa actgcaccga agtggagctg gccacactga cgtgggtgaa ccggtacaat
     1201 aacagaagac gactgggtca tatctcacca gcagaagcgg aacaggcata ttatgcctcc
     1261 taaacagtgt tctggcagct tgagttctca catttaacac tctcccgaaa gaacggggcg
     1321 attcatttca caaatatact gatgtagccc cctgaaacac cagacagtag ctgtatctcc
     1381 agataagaga taggcttgaa tatatgtcta acactaacac caattttgag atgaccggga
     1441 tcctgttagg gcaagaagtc cgtaaacgta aaactcctca ggagaagatc gccattatcc
     1501 agcagacgat ggagccgggt atgaatgtct cccatgtcgc ccgcctgcat ggtatccagc
     1561 ccagcctgct gtttaagtgg aagaagcaat atcaggaagg cagcctcacc gccgttgcgg
     1621 ctggagagga agtcgttcct gcttctgagc ttactgctgc tctgaagcag gtccgggagc
     1681 ttcagcgcct tctgggcaag aagacgatgg aagttgagat cctgaaagaa gccgtggagt
     1741 acggtcagtc gcgaaaatgg atagcgcacg cgcccttgtt gccaaaggac ggggaatagc
     1801 catggtcagc cggaccatgg gcgtgtcgcg tgcgcaactg tcactgcgga ttaaccgttc
     1861 tgccgactgg caggacaggc gctgtaaccg gcgtaatgaa gaagcagacg cagaaatact
     1921 gtcggctatc ctcaacatta tcagcgatat gccgagttat ggttatcgac gcgtgtgggg
     1981 catcctgcgc aagcaacgtc gcacagaggg acagccacct gtgaatgcca aacggcttta
     2041 caggataatg agcgagcata acctgttgtt gttgcatcac aaaccagagc gaccgaagcg
     2101 tgaacataag ggcaagatag cggtggcaga aagcgatatg cgctggtgtt cagatggctt
     2161 cgagttcggc tgcgacaacg gcgaaaaact gcgggtaacg ttcgcgctgg actgctgcga
     2221 ccgtgaggcc atagactggg cagcaagcac gggaggctat gacagttcga ccgtgcagga
     2281 tgtgatgctg aggtcggtgg aaaagcgctt cggcgacagg ctgcccgaca cagcggtgca
     2341 gtggctgacg gacaacggtt cagcatatac cgcgcatgaa acgcggaggt tcgccaaaga
     2401 gctgaatctg gagccatgta caacagcggt gagcagcccg cagagcaatg gcatggccga
     2461 acggttcgtg aagacgatga aggaagacta tatcgcgttc atgccgaaac cggatgtgag
     2521 aacagccctg cgaaaccttg cagcggcgtt cacgcattac aatgaaaatc acccgcatag
     2581 tgcgctggga tatcactctc cgagagaata ccggcggcag cgggcatcgt taacttaaga
     2641 tacaaaagct gtccggaaat ggcgggtcaa gatcacattt cacaaatata cacataaaaa
     2701 aactatattc cgaaatggtt cccccccata ttaatctctt tcatataaaa ttttttcaga
     2761 tacagcaatg agtaaaaaac tcgagcgacc tcctgcaccg acgactattc atcctatctg
     2821 gctaaggctt tgtcattggc tgaatgcgct ggcgatgctg attatggtca ccagcggctg
     2881 gcgcatttat aacgcctctc cactgtttaa ttttcagttt gccaatgaac ttaccctcgg
     2941 cggctggcta ggtggcgccc tgcagtggca cttcgccggg atgtggctgt tatcggttaa
     3001 cggagtaatt tacctgctgt gtaatttttt cagcggccgc ttcaggcaga aatattggcc
     3061 cttatcgcct cgtggattct tcaaggatgc ctttgcggcg ctacggggaa aattagccca
     3121 caccgacctc agccattaca acatggtgca aaaggtcgcc tatttgttcg ttattgccga
     3181 cagcgtgctg ctggttgttt ccggcctagt tgtatggaaa tctgtgcagt tttccctatt
     3241 acgggagcta ctcggaggct acgacatagc tagatttatc catttttacg ccatgtccgc
     3301 aatggttggt ttcgtggtag ttcatctgct gatggtagct ctggtaccac gcacgttgct
     3361 cgccatgctc cgtggtcgct gaggaagaaa tatgagcgat aaaaacgata ttatcaaaga
     3421 agcgactctg cttattaata aacagctgac tcaggaaggc cgtcgccgat ttttaaagca
     3481 gggcttaacg ctcggcggca ttgcgatgtt aacgggctgc gatatcagcg ataacgcaac
     3541 ggtagaacgc tcgcttagta atatttcacg ttttaacgac cgtattcagg cttggctgtt
     3601 taacgacatc cagttggcgc cggtttatcc ggaaagcatg atcgccacaa actttccgtt
     3661 taacgccttt tacggtgagg atgaagcacc tgagatcaac ggcgacaact atagccttga
     3721 gcttgctggc ttgattacca acaaacaccc gtggacgctc gcgcagcttc atcagatggc
     3781 tcaggtaagc caagtcaccc gtcatatctg tgtcgaaggc tggagcgcca tcggcaagtg
     3841 gggcggcgtg cctttttctc tgtttctgaa agttatcggc gcagatctgc gtgccaaata
     3901 cgtgagtttt aaatgtgcgg atgactacta caccagcata gatatggcta ccgcgctgca
     3961 tccacaaacg cttattgctt tgacatggga cggcaaagtt ctgccgagaa aatacggtta
     4021 cccgatgaaa ctgcgtatcc caaccaaact aggctataaa aaccctaagc atattaaggt
     4081 tattgaggtt accaatagtt accccggtgg atactgggaa gaccaaggct ataactggtt
     4141 tggtggtagt taattatcta tatctcactg tgaacgtaag gaaatacaga atgaaaaagc
     4201 tctacaccgc cctgctgtgc tcgaccatgc tgttcgggat taccggcgcc aatgcggcag
     4261 actcaatgtc caaagactca atgtccaagg ataagatggg aaaaatgtcc catacctgta
     4321 aagacggcat gcaaaaaaat tgcatgaaaa aagacagtat gaaaaagtaa taaaattagg
     4381 tctacaacac accaattcag aaaaatctca aaaaagcatc cccagttata ccaatagcag
     4441 gggatgcttg actgctgttc tgtacaaata atggatagtt acaaaaggga ttgaaaataa
     4501 gaggccacca ataaggcgga ttttttatcc atacctgtcg gtcactagga tttaccataa
     4561 cgtcgcatta cgcgcaccgc tccggacggg ttcggcgcga atgtggcatc agcccggtaa
     4621 aacagtcagt aaacttcccg ctaaaccacg cattctgcgc cagataaatt tcacccggct
     4681 gcattgctat gcagccgggt gaatggcacc ggctttcagc gggaatctgt gccgaaagcc
     4741 ccgttttatc cggtttgtta cagcgacgga tcgttatacg tccgctggca ctgcgatcgt
     4801 tccagctcct gctcccggat ggtcgtgtaa ttgtcgagca tctgctgccc ctgccaccac
     4861 agaatgcccg cgctagaggc tatcgtcggc catcgggagg ctctgtcgca ttagtgatat
     4921 ggaatcagca aaaattgcgc agcctgtttt tcaggctgcc aacagagaaa attgttctgc
     4981 cgatgacaac ccatccctct gcggatgcag atattcgcag cgccagcatg aatgtagttt
     5041 atgcaggcga aacgccttat ctaagcgtgg taccagccag ataccccagc ggtgaagcgt
     5101 ggagtgatca acgacaatac caagctcagc catcatctct tcgagattgc gcagactcag
     5161 tgagtaagcc agatacgagc gaacacattg agtgttgaaa caggataatg caggcgttta
     5221 aaggattttc gggtcagata catggtcagg taacatccaa aaacagcatg ttacctgacg
     5281 actccttaat gcgataaggc cttactttgt gctcatgcca tctttgacta cttcatcttt
     5341 ggccatgcag tctttggcca tgcagtcttt ggccatgctg tctttggcca tgtggtcttt
     5401 ggccatgctg tctttggcca tgtggtcttt ggccatgtgg tctttggcca tgctgtcttt
     5461 ggccatgctg tctttggcca tgctgtcttt ggccatgtgg tctgctgcac tggcacttgc
     5521 cataccaaaa ataatggtgg agcacagtaa agcgatgtgg acttttttca ttgtgtattc
     5581 ctgaaagcag agggaagagc cgtttaatga tgaacttatg caactagatt cactgtaaca
     5641 ggaaatcact tatcatttat taaacaattg tttaacttta ggcaatgcgc tgaatttgtg
     5701 gaaaaagaaa tgatttccag tctgtcagag ttggctgggt cttgagtgac tttcttgtga
     5761 gataacttcg ctatcgcgga aggttacacc ctgacgccat aaatgcggtg tatccggaag
     5821 cccgtattca actgtgtatc gtgcatatgg tgcgcaacag cctgcgattc gtcttctgga
     5881 aagattacaa ggtcgttatc cgcgatttga aggcgattta ccaggcaccg acggaagaag
     5941 caggcctgca ggcgctggaa gcgtttacca gcgcctggtg acagccgcta ccgttgcagc
     6001 gttccagcag acaccggtgc tcatcctcag tcacccacat tgtgagcatt ttgctgcgtt
     6061 tatcggccaa tccggtctcc tgataacgcc gggacggacg ggaaatcttg tcccggtcgg
     6121 cgtggtgttc ggcaagccgc aggcgcgtca gtggttttta gcgggtttcg gggcgcagcc
     6181 ctgaaccagt cacgtagcgc tagcggagtg tatactggct tactatgttg gcacggatgg
     6241 ggaaatcagt gaagtgcatc atgtggcagg agaaaaaagc ggcaccggcg cgtcagcaga
     6301 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6361 ggctgcggcg agcggt
//
