LOCUS       NZ_CP151193            13458 bp    DNA     circular CON 17-APR-2024
DEFINITION  Klebsiella variicola strain KVTMDU plasmid p6_KVTMDU, complete
            sequence.
ACCESSION   NZ_CP151193
VERSION     NZ_CP151193.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23446385
            Assembly: GCF_038178705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13458)
  AUTHORS   Ota,Y.
  TITLE     Emergence of KPC-2-producing Klebsiella variicola from hospital
            sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13458)
  AUTHORS   Ota,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. July-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.3x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038178705.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 11:53:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,118
            CDSs (total)                      :: 5,998
            Genes (coding)                    :: 5,805
            CDSs (with protein)               :: 5,805
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 68 of 193
            Pseudo Genes (incomplete)         :: 125 of 193
            Pseudo Genes (internal stop)      :: 32 of 193
            Pseudo Genes (multiple problems)  :: 29 of 193
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151193.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13458
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /strain="KVTMDU"
                     /isolation_source="sewage"
                     /db_xref="taxon:244366"
                     /plasmid="p6_KVTMDU"
                     /geo_loc_name="Japan"
                     /lat_lon="35.7014 N 139.7642 E"
                     /collection_date="2021-06"
                     /collected_by="Tokyo Medical and Dental Hospital"
     gene            complement(517..1626)
                     /locus_tag="LQ252_RS30300"
                     /old_locus_tag="LQ252_30305"
     CDS             complement(517..1626)
                     /locus_tag="LQ252_RS30300"
                     /old_locus_tag="LQ252_30305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110219201.1"
                     /translation="MKSYHYQKDKAKFAYKYNDNYAYAYVVSEKPAITAEHHASMVGGK
                     TNRRANRHPHSVAKERTFISNYRRIDFEKKSGTPYLFSLKPLSDVEPETFRKALKGLTR
                     LLRSAGAEYIYMLEWAPYEQTPHIHLVSNIPGETIVDLEAFAERVVSYWRRVMPKVINS
                     RIEQDAKPVTDFRKLFGYMLKTTVHQFNVRAVATGKDWSVISVTGCSRGWGESAFEVIE
                     VSREACMASKRLMKEVVRSRGVQLRKQAKDCDDEKKRAVSEVSPFSIYGLSREESRKLL
                     AQVNASLPLKEHRFLIEMMERYHRAHNLDSQEEKDKVDRILLAKGYDMRDMYPQWEKPK
                     MSMAERYKTVPIYRLLRKTKKAMMTAREK"
                     /name="hypothetical protein"
     gene            complement(1698..2078)
                     /locus_tag="LQ252_RS30305"
                     /old_locus_tag="LQ252_30310"
     CDS             complement(1698..2078)
                     /locus_tag="LQ252_RS30305"
                     /old_locus_tag="LQ252_30310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_133259461.1"
                     /translation="MVNLTRTEVARLIQSKLLNGKRFTPKQFDELLRKHGDHERSRVLE
                     LLRNQWGIPITRDLKGAYYVSESDLRYFYIDPDDVFDEWKAKAKENRDCRKMFRFLKTL
                     LGLAGISPETRTEVLAAVKARI"
                     /name="hypothetical protein"
     gene            complement(2071..2277)
                     /locus_tag="LQ252_RS30310"
                     /old_locus_tag="LQ252_30315"
     CDS             complement(2071..2277)
                     /locus_tag="LQ252_RS30310"
                     /old_locus_tag="LQ252_30315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_142289821.1"
                     /translation="MTQKYLIADDAAAYLNISRARFFDFRRFIPTFPKPVKTRFNEGRP
                     RLVWTPEQLHAFAFSFWGDRSNG"
                     /name="DNA-binding protein"
     gene            complement(3250..3687)
                     /locus_tag="LQ252_RS30315"
                     /old_locus_tag="LQ252_30320"
     CDS             complement(3250..3687)
                     /locus_tag="LQ252_RS30315"
                     /old_locus_tag="LQ252_30320"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_087843274.1"
                     /translation="MAVHTVTEAAKLAGVTRRTIYRYIKQGKLSSVVTDSNTTQIETSE
                     LLRVFGALSQPEGSQVSTGSQVEEPEYVTRLMAEMSQIRDFLQGQQQLLLEDKLETERL
                     REQVTGLQNERDSVSQELDKVKAELEAERGKGFWKRLFRQG"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3840..4409)
                     /locus_tag="LQ252_RS30320"
                     /old_locus_tag="LQ252_30325"
     CDS             complement(3840..4409)
                     /locus_tag="LQ252_RS30320"
                     /old_locus_tag="LQ252_30325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017347036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_179204964.1"
                     /translation="MRYGYARTSTKEQNPERQRVALQEEDCEELFVDQCSGKNADRPQL
                     QLMMSKLRAGDTVVVKSVDRLARNTKDLLALLDEAKNKGAQVRFIDNRLTFDDSPTSNF
                     MITMLGAVGELERSFIRQRQSEGIEIAKSNNVYKGRPVSKKTEAAKVKIAEKLARGVFT
                     DKEIYESVKVSRASFYRIKKEIQTEQ"
                     /name="recombinase family protein"
     gene            4744..5906
                     /locus_tag="LQ252_RS30325"
                     /old_locus_tag="LQ252_30330"
     CDS             join(4744..5005,5005..5906)
                     /locus_tag="LQ252_RS30325"
                     /old_locus_tag="LQ252_30330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_087759146.1"
                     /translation="MTKSATVSKKPRKQHSPEFRSEALKLAERIGVAAAARELSLYESQ
                     LYTWRSKLQQQKTSSERENELAAENARLKRQLAERDEELAILPKGRDILRKAPEMKYVF
                     IEKHQAEFSIKAMCRVLRVARSGWYAWRIRRLQLSPRQQFRRQCDKAVSQAFHQAKQRY
                     GAPRITDELREQGVKYNIKTVAASLKRQGLRAKAARKFSPVSYKPHNLPVFDNLLNQDF
                     TASAPNQKWVQDITYIRTDEGWLYLAVVIDLWSRAVIGWSMSSRMTARLVCDALQMALW
                     RRKRPKGVIAHSDRGGQYCSADYQALLKQHDLRGSMSAKGCCYDNACAESFFHTLKVEC
                     IHGERLISRDDMRTMVFNYIECDYNRWRRHSACGGLSPEQFENQNLA"
                     /name="IS3 family transposase"
     gene            complement(6306..6911)
                     /locus_tag="LQ252_RS30330"
                     /old_locus_tag="LQ252_30335"
     CDS             complement(6306..6911)
                     /locus_tag="LQ252_RS30330"
                     /old_locus_tag="LQ252_30335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            7006..9903
                     /locus_tag="LQ252_RS30335"
                     /old_locus_tag="LQ252_30340"
     CDS             7006..9903
                     /locus_tag="LQ252_RS30335"
                     /old_locus_tag="LQ252_30340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_023307208.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEVMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            10035..10688
                     /locus_tag="LQ252_RS30340"
                     /old_locus_tag="LQ252_30345"
     CDS             10035..10688
                     /locus_tag="LQ252_RS30340"
                     /old_locus_tag="LQ252_30345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341723208.1"
                     /translation="MHSKFEQERAEDELIYGELEKEFALDGKEFNRYSNDVQTLFKLRK
                     DIKAFDRGNIPREFSDKKKFLYAKSFLFALDSFDRFLGVLSKENGAPNSLTSIHNRINQ
                     EFPGLRGVRNSTHHLEDRSRGLGDFDRKTKKMKVLQLKPITNSAINAPLGNALILNSLF
                     GTKYSCTMADGHLGEVDVSAESLSKVHKILSDTLSSFSWKGSPEIYPNPDFRYN"
                     /name="hypothetical protein"
     gene            complement(11042..12580)
                     /locus_tag="LQ252_RS30345"
                     /old_locus_tag="LQ252_30350"
     CDS             complement(11042..12580)
                     /locus_tag="LQ252_RS30345"
                     /old_locus_tag="LQ252_30350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISKpn24 family transposase"
                     /protein_id="WP_004201219.1"
                     /translation="MNSLLPDDIDELKRLLAEQEALNRALLEKLNEREREIDHLQAQLD
                     KLRRMNFGSRSEKVSRRIAKMEADLKQLQKESDTLTGRVDDPAVQRPLRQTRTRKPFPE
                     SLPRDEKRLLPAASCCPECGGALSYLGEDAAEQLELMRSAFRVIRTVREKHACTQCDAI
                     VQAPAPSRPIERGIAGPGLLARVLSSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDA
                     CCRLLSPLEGALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNAGSELA
                     PAVWFAYSPDRKGIHPQSHLAGFSGVLQADAYAGFNELYRNGQITEAACWAHARRKIHD
                     VHVRTPSALTEEALKRIGELYAIEAEIRGMPAKRRLAERQQKAKPRLKSLESWLREKVK
                     TLSRHSELAKAFTYVLNQWPALAYYTDDGWAEADNNIAENALRMVSLGRKNYLFFGSDH
                     GGERGALLYSLIGTCKLNGVEPESYLRHVLDVIADWPINRVSELLPWRVALPTE"
                     /name="IS66-like element ISKpn24 family transposase"
     gene            complement(12629..12976)
                     /gene="tnpB"
                     /locus_tag="LQ252_RS30350"
                     /old_locus_tag="LQ252_30355"
     CDS             complement(12629..12976)
                     /gene="tnpB"
                     /locus_tag="LQ252_RS30350"
                     /old_locus_tag="LQ252_30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000612626.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVFI
                     FRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDWRQ
                     PKRLLTSLTML"
                     /name="tnpB"
     gene            complement(12973..13377)
                     /locus_tag="LQ252_RS30355"
                     /old_locus_tag="LQ252_30360"
     CDS             complement(12973..13377)
                     /locus_tag="LQ252_RS30355"
                     /old_locus_tag="LQ252_30360"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_003031976.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGINDN
                     LLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPATSPRSQPVAAAAPESLNIS
                     CEVTFRHGSLRLNGAISENILNLLIRELKR"
                     /name="transposase"
ORIGIN
        1 tttttacaag gattgagcgg cgtaattcat cctgttccca tcttatagat aaaaaagaat
       61 ttttcaccag accttgaatt gcaaggcttc caagacgttt tcgcccctca agccgcttct
      121 ggcttgcttt ggatgggtat caagatgtga tcaaatctct cttatagata ctgggtacgg
      181 acatcaaaaa ttgacgtttt aacgtcaagt ggaaagtata agaccgtagc caccaatcca
      241 gtttctacca aaggatgccc ttcgaactca gtccattttt ttctaggact ttggttactc
      301 ttcccgttcg gtttcaactt tggcgcgaag cgccgttatt gactcggaac gcttcgcgcc
      361 attggcggtg aaaccgccct gagcgcaagc aaatcgctac cagggttaac gtcttccttc
      421 tctcgctggt ggcggtgaaa ccgccgtttg cgactgcctt ttcgatgcat ctctccgggt
      481 caactgtcat tccttcacac tatcaacaga aactgctcac ttctctctgg ctgtcatcat
      541 cgcttttttg gttttgcgta atagcctgta tatcggaact gttttgtatc gttcagccat
      601 gctcatcttt ggcttctccc actgggggta catgtcacgc atatcatagc ccttagcaag
      661 tagaatccga tccaccttgt ctttctcttc ctgagagtcc agattatgcg ctcggtgata
      721 ccgttccatc atttcaatca gaaaacggtg ctctttcagt ggcagacttg cattgacctg
      781 agcgagaagc ttcctagact cttcgcgtga cagcccgtag atactgaatg gtgatacctc
      841 tgagacagct ctctttttct cgtcatcaca gtctttagct tgctttcgaa gctgcacacc
      901 tctggatctt acgacttcct tcatcaagcg tttggatgcc atacaagctt ctcggcttac
      961 ttcaattact tcaaaagctg actcccccca gcctcttgaa cagcccgtta cgctaatcac
     1021 agaccagtcc ttgcctgtcg ctactgctct gacgttgaac tgatgaactg tggtcttcaa
     1081 catataacca aacagcttac ggaaatcagt aacaggtttg gcgtcctgtt cgattctgga
     1141 attgattacc ttcggcatta ctcttcgcca gtagctgaca actctctcgg caaatgcttc
     1201 taggtctaca atcgtttctc cggggatatt ggaaacgaga tggatatgtg gcgtctgctc
     1261 gtacggagcc cactcaagca tatagatata ctcagctcca gcgcttctaa gaagtcttgt
     1321 cagtcctttc aatgccttcc tgaaggtttc tggctccaca tcacttaatg gcttcagaga
     1381 gaagagatac ggtgtacctg attttttctc aaaatcgatg cggcggtaat tgcttatgaa
     1441 cgtcctttcc ttggcgacgc tatgggggtg acggttggct cttctgttgg tttttcctcc
     1501 caccatagat gcgtggtgtt cagctgtaat ggctggcttc tcactgacta cataggcgta
     1561 cgcatagtta tcgttgtatt tgtaggcaaa ctttgcctta tctttctgat aatgatatga
     1621 tttcacagta ttttccagaa tacattcaag ttgtaagcat caaaaacccg ctttgagaga
     1681 gcgggttttt ttgtgtctta aattctggct tttactgctg ccagaacctc agttctcgtt
     1741 tcaggactaa taccagccaa acccagcaac gtttttagaa aacgaaacat cttacggcaa
     1801 tcacgatttt ccttggcttt tgccttccac tcatcaaaca catcgtcagg gtcaatgtag
     1861 aagtacctca aatcgctctc agaaacgtag taggcgcctt ttaaatcacg ggtgatagga
     1921 atgccccact gattacgtaa aagctcgaga acgcgactac gctcgtgatc tccatgctta
     1981 cgcaacagct catcaaattg ctttggcgtg aaccttttac cattcagcaa tttggactga
     2041 atcaaacgag caacttcagt tctggtcaga ttaaccattt gaacgatccc cccagaaaga
     2101 aaatgcgaaa gcgtggagct gttcaggcgt ccatacaagg cggggtctac cttcattgaa
     2161 tcgtgttttg acaggcttcg ggaatgttgg aatgaatcgg cggaaatcga aaaatcgagc
     2221 gcggctgata ttgagatagg cggctgcatc gtctgcaatg aggtatttct gggtcatgat
     2281 catttccttt tgaatgttac tgcctacatc ctgtaagcag tgtcgggctt cctgcctgac
     2341 aatgtcagaa taacaataaa atacagacat ttaaagtatt actgtcttac ttttcctcct
     2401 tcaatctaaa tgcgcgaaaa ttattagtga gcttagtggg tgaaaaaact catcccgcaa
     2461 aaatcatcag agcgattgtg agtggttttt tttggggagg taagacgtat gttttgagtc
     2521 tcaggcagga aaatcgcgca ccgctcagat aggctgtgcg cgcaatatca cgttatggtt
     2581 aatgggctat tttatcttgc accaccacca ccagggcgag agacggcaga acgaccaccg
     2641 cagcgagatc catcggcagc ggtatcatta cagaaggctt cgcccctcct gacgagcatc
     2701 acaataaagg tgctcaaatc aagagtaggc gaaacccgac acagcttaaa gatcctcacc
     2761 gtttccgata agtagctccc tcgtgcgctc ctgtgttccc accttaacgt ttacagccag
     2821 ctttgccgct ctttctactt tgggggcgct aactctcagc ttcccggtgc tggcactgct
     2881 cgtcttaggt ggttcgctcc aagctggatg atatgcacga actccccgtt tcatccgacc
     2941 gttgcgcctg ctgggtaact attctcatga ttccaacccc agcggacacg gcaaaaagcc
     3001 gctaatggca acagccaata cggtaacgac taatcagggg aaggtaggtc ttgcagattg
     3061 cgataagcgc aagtagactt gaattacagt tttggtgacg tttgttcccc cgaacatcgt
     3121 tacctggtta atcagtcctg agaaactgat taacccgtca aaacccgcgc ctgagaacgc
     3181 gggttttttc gtttctgagc aagagactac gctgagaaac aaaatataac aagagccaca
     3241 ccaccaaact tagccttgcc taaacaaacg cttccaaaaa cctttcccac gttcagcctc
     3301 aagttccgcc tttaccttat ccagctcttg agaaactgaa tcacgttcat tctgcaaccc
     3361 ggtcacctgt tctcgcagcc tttcagtctc aagcttatcc tcaagcaata actgctgctg
     3421 cccttggaga aagtccctta tctgtgacat ttcagccatt aatcgtgtga catactcagg
     3481 ctcttcaacc tgtgatccag tggatacttg agagccttct ggttgtgaca gggcgccaaa
     3541 aactcgcaat agttcactgg tctcaatctg ggttgtgtta ctgtctgtta caactgatga
     3601 gagcttaccc tgcttgatat aacggtatat tgtccttcta gtgacacccg ctaatttagc
     3661 agcctcagtg accgtgtgta ccgccatgtg acaccccttg ttagctaagt gagacagtat
     3721 gtgaccatgt cacaaacaag atcactaaaa accattatta aatagatggc tcaacagtgc
     3781 cattcttacc gttgatagct ttgctgatag tggtgcgagc cgcccgcctc actacgcctt
     3841 tactgttcgg tctgaatctc ttttttaatc cggtagaagc ttgcccggct gactttcacc
     3901 gattcgtata tctctttgtc tgtgaataca cccctggcaa gtttttcagc gatcttcacc
     3961 tttgcggctt ccgttttctt actgacaggt ctgcccttgt atacgttgtt tgatttagcg
     4021 atctctatcc cttcgctctg gcgctggcgg atgaatgagc gctcaagctc ccccacggct
     4081 cccagcatcg taatcatgaa attactggtt ggtgagtcgt cgaatgtcag acggttgtcg
     4141 atgaatctca cctgtgcgcc tttattcttg gcttcatcaa gtaacgctaa caggtctttc
     4201 gtattgcgtg cgagccggtc tacagatttc acgacaaccg tatcaccagc tcttagcttt
     4261 gacatcatga gctgaagctg tggacgatct gcgttttttc ctgagcattg gtctacgaat
     4321 agctcctcgc agtcctcttc ctgcaaggct acccgctggc gttctgggtt ctgctctttt
     4381 gtcgatgttc tcgcgtatcc gtatctcatg attgttgccc tgggtatcaa ttaattgacc
     4441 atcaagatac ggtctcatat gagttatgta cagactatta tgataccgtc tttcacgctc
     4501 atctcctggg tctcacatgc ctgtacccaa aaaacactat attcaatgac cctttaaaaa
     4561 cctaccgcct cgccaagctt gttggtcaac gctgcacgaa accggattaa aaatccgttc
     4621 ccgttcaccg tttcagctag gaaggaaagg tcggtttttg aggggctgag aggcgttttg
     4681 atcctacccg cgaatagtgg acacgcgact aagtgagtaa actcccagac agaggtatct
     4741 cacatgacta aatcagcaac cgtaagtaag aaaccacgta aacagcattc gcctgaattc
     4801 cgcagcgaag ccctgaagct tgcggagcgc atcggcgttg ccgctgccgc ccgggaactc
     4861 agcctttacg agtctcagct atatacctgg cgcagcaaat tacagcagca gaaaacgtct
     4921 tccgaacggg aaaatgagct ggccgctgaa aatgcccgcc tgaagcgcca gctggcagag
     4981 cgtgacgagg agctggctat cctccaaaag gccgcgacat acttcgcaaa gcgcctgaaa
     5041 tgaagtatgt cttcatcgaa aaacatcagg ctgagttcag cattaaagcc atgtgtcggg
     5101 tgctgcgcgt ggcccgcagc ggctggtatg cctggcgtat ccgtcgcctg cagctaagcc
     5161 cgcgtcaaca atttcgccgg caatgtgaca aagcggtcag tcaggccttt catcaggcga
     5221 aacagcgcta cggcgcaccg cgcattactg atgagctgcg cgaacagggg gtgaaataca
     5281 acatcaaaac ggtagcggcc agcctgaaac ggcaggggct aagggccaaa gccgcgcgta
     5341 agttcagtcc ggtgagttat aagccccata atctgccggt attcgataat ctgctgaatc
     5401 aggatttcac cgccagcgca ccgaatcaga agtgggtgca ggacatcacc tacattcgca
     5461 ccgatgaggg ctggctgtat cttgccgtgg tgattgacct gtggtcccgc gcggttattg
     5521 gctggtcgat gtcatcccga atgacggccc ggctggtatg cgatgccctg caaatggcgc
     5581 tgtggcggcg taagcggcca aaaggggtta tagcccacag cgaccggggt ggtcagtact
     5641 gctcagcaga ttatcaggcc ctgttgaagc agcacgacct tcggggcagc atgagtgcaa
     5701 aaggctgctg ctatgacaat gcctgcgcgg aaagcttttt ccacacgctg aaggtagagt
     5761 gcatccacgg tgagcgtcta atcagtcgcg atgatatgcg tacgatggta tttaattaca
     5821 ttgagtgcga ttacaatcgc tggcgtcgtc acagtgcctg tggcggcctc agcccggaac
     5881 aatttgaaaa tcagaatctc gcttaggcgc gtgtccatat tacgaggaga ggatcatttt
     5941 tgcctgaaat ctgttatcaa tcagtccagt tcaacgattc attcccgtaa actcaccagg
     6001 gcgattctga ggcgttttga gagggtaagg cgtggagtta ttgagggtta gcgcttagaa
     6061 acacgcacag cgcgtctgag cggtgcgcgt aagaagggat tatggtaaat gtattcttcc
     6121 tagaagataa ggtgtttcca catacttaat gaagtgagtc agccatgatt atctttcaag
     6181 ttgtaaaacc tggtgtttgg ttggacggta tagccgatag acagactagt tttaatattg
     6241 aaaatttaat tagaaatcta attgatgttt ttggggtcgg ttccggctga gggcgaaatg
     6301 acaccctaag cgttagctct gtgtcgttgc acgatgtcag cgacggtatt cttgctgata
     6361 ccgagctcgc gtgcgatcca gcgatagctg cgtccctcgg ccctcatcgc aaccacctta
     6421 ggcaaaagtc ggtctgattt tggtcgcact ccggcctgac gaccaagcct cttaccacgt
     6481 gccttcgcaa cagcaaggcc tgacttgacc cgctcgctga tgagatcccg ctcaaactcc
     6541 gcaatgccgg aaagaaacgt cgccagcatt cgtccatacg gcgacgaaag atcgaacgcc
     6601 attccattca tggctatcac ggaaaccttc cagttctcca gttcacgtag cgtattgagc
     6661 agatcgagcg tcgagcgccc ccaccgggaa agctcagtga ccaggattgc atcaatttgt
     6721 ctggactggg caagcgccag gactttcttt cgctcggccc ggtcgagttt agttcctgaa
     6781 cctgtttcct taaatattcc caccacgtcg tagccggcac ggccggcgaa ggctcgcaga
     6841 tcaaattcct ggcgttcaca agactgatcc gctgttgaaa cccggcagta aatggcggca
     6901 cgatgtccca attgaaccct cctggatttt tgtatcggaa cgccctgatt tatatgggct
     6961 ggctgttgtc caaaacagac tatacttcaa aagggacgaa tttgtatgtc acgacgccat
     7021 attttcaccg aacggcagcg agcagcgctg ttcgatctgc ccacggacga actgtcgcta
     7081 ctgaagttct acacgctggg cgatgatgac ctggaaaaca ttaggcagcg ccgcagaccg
     7141 gaaaacagga ttggctttgc cctgcaactt tgtgccttac gatatccggg ccgtgcactg
     7201 gctcctggtg agatgatccc gcgtgaagtc ctttccttcg tcggtgctca gcttggagtt
     7261 ccggctgatg cgcttctcac ttatgccaca cggcgccaaa cccgtcagca gcacatggac
     7321 acgctgcgcg aaatttacgg ctacaagacc ttcacgggcc gtggtgcccg tgatctgcgg
     7381 gagtggactt tcggccaggc cgaagatgcc agatcaaacg aggatcttgc tcatcgtttt
     7441 attgtgcggt gtcgggaaac ttccaccatt ctgcccgcag tatcgacaat cgagcgcttg
     7501 tgcgcggatg ctctggtcgc cgctgagcgg cggattgaaa cgcggattgc ggaaaattta
     7561 acagcggatg ttcgcgatca cctggacaaa cttctgagtg aaatgctcgc cggcaatatc
     7621 agtcgtttca tctggcttcg caacttcgag gttggtaaca actcggctgc tgctaaccgt
     7681 ttgctcgaca ggctcgaatt tctgcgtacc ctgaatatca atcatagtgc tttggccagc
     7741 atacctgccc atcgcattgc ccggctgcgt cggcagggtg aacgctactt caccgacggt
     7801 ttgcgtgaca tcacttcgga ccgccgctgg gcgatccttg ccgtctgtgt tgtggagtgg
     7861 gaagcggcga ttgctgatgc catagtcgaa acccatgaca ggatcgtagg aaaaacctgg
     7921 cgggaagcga agcgccagca tgacgaaaca atttccggct ctaaagccac actcacggat
     7981 acgatccgta ccttcaccgc gctgggagct tcgttgcttg aggcccgcag tgacggaacc
     8041 ccgctggaga tggctgtcgc cagttcggtt gcatgggacc ggctcgctca actggtagcg
     8101 acagggactc aactcagcaa cacgctagcc gatgagcctc ttgcatatgt cgggcaggga
     8161 taccatcgct ttcgtcgtta tgcgccccgc atgttgcgct gtctgaagct cgaagccgcg
     8221 ccggtcgccg gaccattggt agcagcagct ttgtcgatcg gagagatgaa aggtgttgca
     8281 tcgccagaaa ggcgtttcct gcggcccagc tccaaatgga accgtcattt acgagctcag
     8341 gaaaaaggag atacccgtct ttgggaagtg gcggtactct ttcacctccg ggatgctttt
     8401 cgttccggag atgtctggct cgctcattcg cgccgctatg gtgacctcaa gcaggtactg
     8461 gtgccgatga tcgcggcgca ggaaaatgca aaactggccg tgccttccaa cccacaggat
     8521 tggctggcag acagaaaggc gcgactcacg atcgctctta agcggctggc ccgggctgcc
     8581 cgtaacggca ctattccgca cggtagcata gaagatggaa cgttgcggat cgacaggttg
     8641 acagcagacg tgccggatgg tgccgaggca ctcatactgg atctgtatcg ccgaatgccg
     8701 tccgttcgga ttaccgacat gctgcttgaa gttgatgcag cccttggttt cacagatgcg
     8761 tttacccatc tgagaaccgg ggctccatgt cgcgaccgga tcggtctgct caacgtcctg
     8821 ctcgctgaag ggctcaatct gggcctgcgt aagatggcgg aagctacaaa cacgcatgat
     8881 tactggcagc tctcacgcct tgcccgctgg catgttgaaa gcgaagccat gaaccaggca
     8941 ttggcaattg tggtggccgc gcagggtaaa ctgccgatgt cacgcgtctg ggggatgggc
     9001 acgtcagcat cgagcgatgg tcagtttttc ccgacagcgc ggcatggcga agtcatgaac
     9061 atggtcaatg ccaaatatgg ttctgttccc ggcctcaaag cgtatactca cgtaagcgac
     9121 cagttcgcgc cattcgcttg tcagtcgatc ccggcgaccg tgagcgaggc accgtatatt
     9181 ctcgatggac tactgatgaa cgaggtcggt cgccatgttc gcgaacagta tgccgataca
     9241 gcaggattca ccgaccattt gttcggagcc agtagcctgc tcggctacaa tctcgttctg
     9301 cgaatcaggg atctgccatc gaagcggttg tacgtattta atcccgatac gacccccagg
     9361 gagttacgca agttggtagg tggaaaagcc cgggaggatc ttatcgttgc gaactggcct
     9421 gatattttcc gttgtgccgc gacgatgacc gctggcaaaa tcaggcccag ccaactcctg
     9481 cgcaagctcg cttcttaccc acgacaaaac aaccttgcag ttgcgcttcg tgaagttggt
     9541 cgtattgaac ggaccctttt cattattgag tggatcctgg atacggacat gcagcggcgt
     9601 gctcagatcg gtcttaacaa gggagaggcc caccatgcgc tcaaaaatgc gctccgtatc
     9661 gggaggcagg gggaaattcg cgatcgcacg acagaggggc agcactaccg aatcgctggg
     9721 ctcaatttat tgactgcggt gatcatttac tggaataccg tccatcttgg tcatgccgtc
     9781 acggagcggc ggaacgaagg gttggatgtt ccccctgaat ttcttcccca catatcccca
     9841 ttgggctggg cgcacattct actgactggc gaatatcttt ggcccaagga accgaaagct
     9901 tagggtgtca tttcgccctc agccggaacc gacccctttt ttacgaggca aatttaagcc
     9961 ttaatctctt caatgaagaa atggattctt ttcgacaaag gatggagctt cgtacaaccc
    10021 ttagtcatga actaatgcac tctaagtttg aacaagaaag ggcagaagat gagttgattt
    10081 atggtgaact agagaaggag tttgcattag atgggaaaga gttcaaccgc tacagcaatg
    10141 atgtgcagac tctgttcaaa ctacggaaag atatcaaagc ttttgatagg gggaatattc
    10201 cacgagagtt tagcgataag aagaagtttc tgtatgccaa atcgttcctt ttcgctctag
    10261 attcttttga ccgttttctg ggggtcctat ccaaggaaaa cggtgcgcca aattcattaa
    10321 cctctattca taaccgaata aatcaagaat ttccggggtt aagaggagtt agaaatagca
    10381 ctcaccatct agaagaccga tctagagggc taggagactt tgaccgtaag actaagaaaa
    10441 tgaaagtact gcaactaaag ccgatcacga atagtgcaat taacgcacca cttggtaatg
    10501 ctttgatttt gaatagcctt tttggaacta agtatagctg cactatggct gatggacatt
    10561 taggggaagt tgatgtttct gctgaatccc tatcaaaagt acataaaata ctaagcgata
    10621 ctctttcctc ctttagctgg aaaggaagcc ccgaaatcta ccctaatcct gattttaggt
    10681 acaattaacc taatgccccc gtggtgggta tatgtaaaaa ccacggggag cgtagcgacc
    10741 atcgtcatgc tcgtttcttg tgtgtctcac cctgcaaaag gtttgattgt gtgggcgcag
    10801 tgttcgacgt tccaaatcac actgttccgc ttgttgctgc cttggatgct cgttttatca
    10861 ggtgccagtt atcgtactgc gagacggtgt agagcctaac tctttcgcgg ttaagttacg
    10921 tttccaccgt ttgcctgttt gtcctgccgg agtccggggc gtagacctga gtgtcaaagc
    10981 aaaacaccag cggcgatttt tacagtaagc gtgcagcgag aaccgtattg acggggatgt
    11041 gttattcagt tggcagtgct acgcgccagg ggagcagttc gctgacccgg tttatcggcc
    11101 agtcagctat aacatcaagc acatggcgaa ggtagctttc tggctccact ccgttcagtt
    11161 tgcatgtccc gatcaggctg tacagcagcg ctccccgctc tcctccatga tccgaaccga
    11221 agaacaggta gtttttgcgg cccaggctga ccatccgtag cgcattctca gctatgttgt
    11281 tatctgcctc ggcccagccg tcgtcagtat agtaagccag cgccggccac tggttcagta
    11341 cgtacgtgaa cgctttcgcc agttctgagt gtcgcgacag cgttttcacc ttttcacgca
    11401 gccagctttc cagggatttc agccgcggtt tagctttttg ctgacgttct gcaaggcgtc
    11461 gcttcgccgg catccccctt atttccgcct cgatggcata taactcaccg atccgtttca
    11521 gggcttcctc cgtcagtgct gacggggtgc gaacgtgcac atcgtggatc ttgcggcggg
    11581 catgagccca gcaggcagct tccgttatct gtccattgcg gtacagttcg ttgaacccgg
    11641 cgtacgcatc cgcctgcaga acaccgctga agccagcgag atggctctgc gggtggatac
    11701 cttttctgtc cgggctgtaa gcgaaccaca ctgccggcgc cagctctgac ccggcgttac
    11761 ggtcgtcacg aacgtacgtc cacaaccgcc cggtcttcgt tttcttatta cctggcaaca
    11821 gcaccgggac aggcgtgtca tcagcatgga gcttaccgtc agtcagcaca tagtcctgaa
    11881 gcgccccttc cagcggtgac agtagccggc aacacgcatc cacccagccc gacagcagtg
    11941 agcggctcag ctccacaccc tggcggccgt atatttcaga ctggcggtac agcggggtgt
    12001 gctctgcata ctttgaactc agcacgcggg ccagcagccc cggtcctgcg ataccccgct
    12061 cgatgggccg tgaaggcgcg ggggcctgca cgatggcatc gcactgagta caggcatgct
    12121 tttcacgtac tgtccggata acccggaagg cgctgcgcat cagctccagc tgttcggcgg
    12181 catcttcacc caggtaactc agcgcaccac cacattccgg gcagcatgac gctgccggca
    12241 gcagccgttt ttcgtcacgg ggaagtgatt cagggaacgg tttgcgggta cgggtctgcc
    12301 gcagcgggcg ctgcacggcc gggtcatcca cccggccggt aagggtatcg ctttctttct
    12361 gcaactgctt aaggtcggct tccatcttcg cgatacggcg ggagactttt tcggagcggc
    12421 tgccgaagtt catccggcgc agcttatcca gttgcgcctg caggtgatct atttcgcgtt
    12481 cacgctcgtt cagcttttcc agaagggcac ggttcagcgc ctcctgttcg gcaaggagac
    12541 gtttcagttc atcgatatcg tcaggaagta agctgttcat accgggtata ttaccaggct
    12601 cattcagcgt cgaccaggat aaagaggcct acaacatagt cagtgacgta agcagtcttt
    12661 taggctgccg ccagtcgata ccttcaagga gcatcgccag ctgtgccggt gtgaggaaca
    12721 ctttgccatc ccgggctgac ggccaggcga agcggccgcg ctccagccgt ttggtcagca
    12781 gacacagtcc atcgccggta gaccagagga gctttacctg actgccatta cgcccacgga
    12841 agataaaaac gtgacctgac atcggatcgt ctttcagcgt cgtctgcacc tttgccgcca
    12901 ggccgttgaa gccgtttctc atatcggtga tgccagcgac cagccagatc tttgtccctg
    12961 atggtaatgg gatcaacgtt tgagctcccg tatcagcagg ttcaggatat tttcgctgat
    13021 ggcaccattc agacggagtg atccgtgccg gaacgtcact tcacagctga tattgagtga
    13081 ttcaggtgcc gcagcagcta caggttgtga ccggggggag gttgcaggga caacatcttc
    13141 ggcatcaagt gttatcggga gcagctcagg cacgttgttt tctgttgttg aaggaggacg
    13201 tagttttcct tcgcgccagt actggcgcca cttgaacagc aaattatcgt tgatcccatg
    13261 ctcacgagcg agttgcgcta cggagatctc tggtcgatgc gagagttcaa ccattttgat
    13321 tttgaactca acgggataat tagggctttt tttacgcact gcggttaatg atttcatgga
    13381 tagcgtccac catatttggt gtccattatc ctctcaggaa tttcaggatc tgccagacgg
    13441 tgctgagacg acgcttac
//
