LOCUS       NZ_CP151149            18969 bp    DNA     linear   CON 16-APR-2024
DEFINITION  Staphylococcus aureus strain BSN152 plasmid pSR02.
ACCESSION   NZ_CP151149
VERSION     NZ_CP151149.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637731
            Assembly: GCF_038096285.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18969)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN152 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18969)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN152
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP030326, CP048645, CP053009, KT780704,
            CP053637, CP014405
            Genome Coverage                   :: 174.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096285.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:59:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,988
            CDSs (total)                      :: 2,906
            Genes (coding)                    :: 2,817
            CDSs (with protein)               :: 2,817
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 47 of 89
            Pseudo Genes (incomplete)         :: 41 of 89
            Pseudo Genes (internal stop)      :: 32 of 89
            Pseudo Genes (multiple problems)  :: 29 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151149.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..18969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN152"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSR02"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-11-03"
                     /collected_by="Andrew D. Berti"
     gene            105..713
                     /locus_tag="UXR25_RS14695"
                     /old_locus_tag="UXR25_14700"
     CDS             105..713
                     /locus_tag="UXR25_RS14695"
                     /old_locus_tag="UXR25_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583537.1"
                     /translation="MIIGYARVSSVDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEVINTVNYLKDKEVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIVQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKNGKGLS"
                     /name="recombinase family protein"
     gene            complement(918..1484)
                     /gene="qacR"
                     /locus_tag="UXR25_RS14700"
                     /old_locus_tag="UXR25_14705"
     ARG             complement(922..1484)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1662..3206
                     /locus_tag="UXR25_RS14705"
                     /old_locus_tag="UXR25_14710"
     CDS             1662..3206
                     /locus_tag="UXR25_RS14705"
                     /old_locus_tag="UXR25_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="WP_000622776.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="quaternary ammonium compound efflux MFS transporter
                     QacA"
     gene            3411..3971
                     /locus_tag="UXR25_RS14710"
                     /old_locus_tag="UXR25_14715"
     CDS             3411..3971
                     /locus_tag="UXR25_RS14710"
                     /old_locus_tag="UXR25_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4294..5061)
                     /locus_tag="UXR25_RS14715"
                     /old_locus_tag="UXR25_14720"
     CDS             complement(4294..5061)
                     /locus_tag="UXR25_RS14715"
                     /old_locus_tag="UXR25_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_001829872.1"
                     /translation="MRTIKMVADELGVTKQTIVNNAKSLNITFEKENGINYIDDNDYLK
                     IIEKITKKESTIQNKESVKKELFGENTAKSEKNIYNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLESQLEEERQLN
                     YSFDTATNNRQNINPQEATFTEESQNINQQQTKRQPTEVNYKDIAEEKKNDSTDEGELT
                     KEDKFVEETKNDPDDNREVEPPKKGFWSRFFGN"
                     /name="DUF536 domain-containing protein"
     gene            5501..6436
                     /locus_tag="UXR25_RS14720"
                     /old_locus_tag="UXR25_14725"
     CDS             5501..6436
                     /locus_tag="UXR25_RS14720"
                     /old_locus_tag="UXR25_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001829877.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSIQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELISFNLLTQERVGLN
                     KPNRLYLLKPNYDIKASHSKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTENNDTD
                     YIKTESNDTNDLNDNKLTFPSNHTNHSNHNNSNFNNEALKFQLLEELPQSIQRYLSNFS
                     VNEIKIIKPVLLKAKTSFNNSIDTYYLLEDMEIEILHVLKRFKAMLIQKNETVEAMQGY
                     LMKSLKSEFAEMHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            8283..8819
                     /locus_tag="UXR25_RS14725"
                     /old_locus_tag="UXR25_14730"
     CDS             8283..8819
                     /locus_tag="UXR25_RS14725"
                     /old_locus_tag="UXR25_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001829875.1"
                     /translation="MISNKLKSARLAKELTQSEVAKKLYVTRQTVSRWEQGKTLPNIYV
                     IQELSDLYDISLDELVVNTALENKEAKSMKNVNPFALFGAILFNVFLFSLIVIFFGGFL
                     LLSLFISLAMLFSPLILAIFVLTGVQEFNVIQLVMTVIFVVIGLLSIPLLKKVSVFLFK
                     FFKRYLKFNHKAIFY"
                     /name="helix-turn-helix domain-containing protein"
     gene            8973..9068
                     /locus_tag="UXR25_RS14730"
                     /old_locus_tag="UXR25_14735"
     CDS             8973..9068
                     /locus_tag="UXR25_RS14730"
                     /old_locus_tag="UXR25_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_014287283.1"
                     /translation="MSEIFVNIMTTAASGCLVALFAHWLRKRNDE"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9403..9762
                     /locus_tag="UXR25_RS14735"
                     /old_locus_tag="UXR25_14740"
     CDS             9403..9762
                     /locus_tag="UXR25_RS14735"
                     /old_locus_tag="UXR25_14740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031774055.1"
                     /translation="MKKMLRISIFIIVVSIFIVFKYSYQIEGILEEYPNILKAFVALFT
                     LLTTVISLVIAYKSYDNSVKQQEKDKEEKNKQFEENSDRIINSLKSDLAKYTRKINVFR
                     LGYTNSGSVANLKII"
                     /name="hypothetical protein"
     gene            9905..10750
                     /locus_tag="UXR25_RS14740"
                     /old_locus_tag="UXR25_14745"
     CDS             9905..10750
                     /locus_tag="UXR25_RS14740"
                     /old_locus_tag="UXR25_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109600.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001829879.1"
                     /translation="MDLVENCNTFLSFVTNRRMDKKKLYKSNPCKNRFCPMCAWRKARK
                     DALGLSLMMQHIKQAEDKQFIFLTLTTPNVTSEHLESEIKHYNQSFRRLSNRKHFKSIA
                     KGYVRKLEITYNKKRDDYNPHFHVLIAVNKSYFKDTKAYITQKEWLEMWRDVTGNSEIT
                     QVHIQKVKQNNNKELYEMAKYSGKDNDYLINQKVFDAFYKALKGKQVLVYSGLFKDARK
                     KLKNGELDYLKEVDPTEYIYQIFYTWNKKEYLASEIFDLTESEKQEINHQMIDEIEEEK
                     "
                     /name="protein rep"
     gene            complement(11599..12084)
                     /gene="lnu(A)"
                     /locus_tag="UXR25_RS14745"
                     /old_locus_tag="UXR25_14750"
     ARG             complement(11603..12084)
                     /name="lnu(A)"
                     /gene="lnu(A)"
                     /gene_name="lincosamide nucleotidyltransferase Lnu(A)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            <12546..13085
                     /locus_tag="UXR25_RS14750"
                     /old_locus_tag="UXR25_14755"
                     /pseudo=""
     CDS             <12546..13085
                     /locus_tag="UXR25_RS14750"
                     /old_locus_tag="UXR25_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            13245..14015
                     /gene="aadD1"
                     /locus_tag="UXR25_RS14755"
                     /old_locus_tag="UXR25_14760"
     ARG             13246..14012
                     /name="aadD1"
                     /gene="aadD1"
                     /gene_name="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            14295..14969
                     /locus_tag="UXR25_RS14760"
                     /old_locus_tag="UXR25_14765"
     CDS             14295..14969
                     /locus_tag="UXR25_RS14760"
                     /old_locus_tag="UXR25_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_044124596.1"
                     /translation="MNHFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            15003..15929
                     /locus_tag="UXR25_RS14765"
                     /old_locus_tag="UXR25_14770"
     CDS             15003..15929
                     /locus_tag="UXR25_RS14765"
                     /old_locus_tag="UXR25_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_049883636.1"
                     /translation="MFFTLQQNHHDLDNFSKTGYSNSRLDAHTVCISDPKLSFDAMTIV
                     GNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWDRRNMRI
                     EFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVKKTIFYG
                     RNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMVDYWNDC
                     FSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISPVDLTDL
                     MKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            16091..17470
                     /gene="tet(K)"
                     /locus_tag="UXR25_RS14770"
                     /old_locus_tag="UXR25_14775"
     ARG             16092..17467
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            17656..18856
                     /gene="mobV"
                     /locus_tag="UXR25_RS14775"
                     /old_locus_tag="UXR25_14780"
                     /pseudo=""
     CDS             17656..18856
                     /gene="mobV"
                     /locus_tag="UXR25_RS14775"
                     /old_locus_tag="UXR25_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829561.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            18886..>18969
                     /locus_tag="UXR25_RS14780"
                     /old_locus_tag="UXR25_14785"
                     /pseudo=""
     CDS             18886..>18969
                     /locus_tag="UXR25_RS14780"
                     /old_locus_tag="UXR25_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     ARG             1663..3203
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(11600..12084)
                     /name="lnuA"
                     /gene="lnuA"
                     /gene_name="lincosamide nucleotidyltransferase (LNU)"
                     /tool="rgi"
                     /drug_class="lincosamide antibiotic"
                     /identity="97.52"
                     /coverage="100.0"
     ARG             13246..14015
                     /name="ANT(4')-Ia"
                     /gene="ANT(4')-Ia"
                     /gene_name="ANT(4')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.21"
                     /coverage="101.19"
     Typing          5502..6436
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.145"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9903..10746
                     /name="rep_cluster_1281"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.321"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          15029..15929
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          17657..18252
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          18249..18835
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.98"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 atatgtacac tttgagtaca ccctaatcat acaaagagag ttattgaatt tataataact
       61 taaacgtatg attagggtgt atattaattg tggagtgaaa atgaatgatt ataggttatg
      121 caagagtatc ttcggtagat cagaatttag agagacagct agataatttg aaaacatttg
      181 gcgtagagaa aatatttaca gagaaacaat cgggaaagtc agtagaaaat agacccgtat
      241 ttcaagaagc acttaacttt gtgagaatgg gcgatagatt tgtagtagaa tcgattgatc
      301 gcttaggtcg taattatgat gaagtgatta acacagttaa ttatttaaag gataaagaag
      361 ttcaattgat gattactagt ttacctatga tgaatgaagt gattggtaat ccactattag
      421 ataaatttat gaaagattta atcgttcaaa tcttagcaat ggtttcagaa caagaacgaa
      481 atgaaagtaa acgtagacaa gcacaaggta ttcaagttgc gaaagaaaaa ggtgtgtata
      541 aaggacgccc tttactatat tcacctaatg ctaaagatcc tcaaaaacgt attatctacc
      601 atcgtgttgt tgaaatgtta gaagaaggca aagcaattag taagattgcg aaagaggtta
      661 atatcacaag acagacagtt tatagaatta aaaatggtaa gggattatct tgataaatgg
      721 attgttaatg caaatgagat cttctcacag cgtcctaaat tatatgttat tcatctatta
      781 tgagattcta ataataagac aagaaaagag agtgcgaatt cgcactctcc ttttgtgtag
      841 tactattgaa attttgtctt aaaaacgaac gtaatttaga aataatattt attggtattt
      901 caagaatata tatataatta tttactaagt ccatttaaaa aaatttgaga gaacttgttc
      961 ataagtttaa ttctttcatt aatattttgt tcatgtgtaa aggtaacaat tccattaaca
     1021 gcgtttgctg ctattttact aacagcatta acatcattaa tacaccattc gccatttaaa
     1081 ttaccctcct taaaaattac atgataagca tctatatatt tgttttctaa tttattcatt
     1141 ttttcattaa tgctattagt tttataatat tcagtgtaaa attcaattat tgcgttttga
     1201 agtggataat agtattcggt tgttaaagat agttcattat ataaataaaa tttttctcta
     1261 ttagttttac atttgatttg ttcctttttc cactgttctt gccatttaga ttcttctata
     1321 tttaaaattt ctaaaaatag attttctttt gttttaaagt gataataaag attcccttta
     1381 ctactttctg ataatttaac aatttctcca gtagtagtgg cattatatcc attttttata
     1441 aataattcct ttgcgacacc tagtatttta tctttcaagt tcatcacgcc tttaatgagt
     1501 attaagtact atcaatgata gcacaaaaaa ttcaaagaat aattgtataa cacaatacag
     1561 ccatttaaat ttcgcaagat tttttgttgt aatatgtaaa aaaatagatt ataatcctta
     1621 tagaccgatc gcacggtcta taaggattgg agggaactta aatgatttca ttttttacaa
     1681 aaactactga tatgatgaca tcaaaaaaaa gatggactgc actagtagta ttagctgtta
     1741 gtttgtttgt tgttacaatg gatatgacaa tattaattat ggctttaccg gaattagtaa
     1801 gagagttaga gccttctggt acccaacagt tatggatagt tgatatatac tctcttgttt
     1861 tagctggctt tataattcca ttgagtgcct ttgctgataa atggggaaga aaaaaagcat
     1921 tattaactgg atttgcttta tttggcctcg tttcattagc tatatttttc gcagaaagtg
     1981 cagagttcgt aatagctatt cgatttttac ttggtattgc aggtgcttta ataatgccaa
     2041 ctactctttc aatgataaga gtaatttttg aaaaccctaa agaaagggcc actgcattag
     2101 ctgtatggtc aatcgcttca tcgataggtg ctgtttttgg accaattatc ggaggagctt
     2161 tacttgagca attttcatgg cactcggcat ttttaattaa tgtaccgttt gcgataatag
     2221 cagttgtagc aggtttattt ttattaccag agtctaagtt atcaaaagaa aagtctcact
     2281 cgtgggatat tccttctaca attttatcaa ttgcaggcat gattggactg gtatggagta
     2341 tcaaagaatt ttcaaaagaa ggactagcag atattattcc atgggttgta atagtattag
     2401 caattaccat gatagtgata tttgttaaac gtaatttatc aagttctgat ccaatgttag
     2461 acgtaagact ttttaaaaag agatcatttt cagctggtac aattgctgca tttatgacaa
     2521 tgtttgcaat ggcatctgtt ttgttattag cttcacaatg gttacaggtt gtggaagaac
     2581 tttctccttt taaagctggc ttatacctat tacctatggc aataggagat atggtgtttg
     2641 caccaattgc acccggatta gcggcgcgat ttggaccgaa aatagtgtta ccttccggaa
     2701 ttggaattgc agccattggc atgtttatta tgtatttctt tggtcatcca ttatcatatt
     2761 ctacaatggc tttagcatta attttagttg gagctggtat ggcttcacta gcagttgcat
     2821 ctgctctaat aatgttagaa acacctacat caaaagcagg taatgcagct gctgttgaag
     2881 agtctatgta tgaccttgga aatgtttttg gtgtagcagt acttggtagc ctatcttcta
     2941 tgctttatcg tgtattttta gatatttcat ctttttcatc aaaaggtata gttggagatt
     3001 tagctcatgt agctgaagaa tctgtagtgg gcgctgtcga agtagctaaa gctacgggga
     3061 taaaacagct tgcaaacgag gctgtaacat catttaatga tgcttttgta gcaactgctt
     3121 tagtaggtgg gattatcatg attatcattt caatagttgt ctatttgtta attcccaaat
     3181 cacttgatat aactaaacaa aaatagtact aaacatatat ttttagatat tgtagattag
     3241 tttattttta aaattagtaa tagttaaaaa gtagaaccat taattttaaa tggttctact
     3301 tttttattat taagtaaaat tataaaagac aatttcttaa attaataata tgatatacct
     3361 atatctaata atttattagt attaataaac cttgaaagga tgattaactg atgaatagag
     3421 cagatatttt taaaatattt gatgtagaac ttaatataac tatattttta ggagcactag
     3481 tagcaacttt catctatttt ataagttaca tgaacaatgt aaatgaatta attacattat
     3541 gcttaacatt agcttttcct gcatatttat taaaaattag cagatcgttc gaggttattt
     3601 tattagagga aaaaataaaa aatagtaaaa tcaaaatctg tgacaaagat agaattgaac
     3661 gagagatact ggtgaaaaag aacctaatag atgaactatt gtttcctaat agagttatta
     3721 aaaaatatac actgacaatt ttaatttaca gtataggtat tattattctt ttactacttt
     3781 gtaatatgtt aagttatgga attggtgaaa atgtcaattt tatctggaga ttactagctt
     3841 gttatgtaac gagtgtcaca gtgattatag ttgccttgat tataatgata atgatatttg
     3901 atcttataat tggattacta ttaaattttc aaatattttt aaaaaataaa aagtatgatg
     3961 actttttata attattcgat ttatataatg aaaaattaaa tgattaaaca tagaaacctc
     4021 aaaacaaacc atatttaata gagtttatgg cgccttatac aaataaaggc tatgctgtta
     4081 aagaattatg tagctatctt cagattaata tggatgatgt ttatactatt ggcgatagaa
     4141 aattgattta cctatgttta ataccctaga taattcttct gataatttag tttttgttag
     4201 agtttaacat aactgatgat tatcagaagt ttcatcacta taaaaggtgt atcaaagatt
     4261 tatactccct tgatacacct tttacattca tttttaatta ccgaagaatc gactccaaaa
     4321 tcccttttta ggtggttcta cctccctatt atcatcagga tcattcttcg tttcttcaac
     4381 gaacttatcc tcttttgtta attcaccttc atctgtactg tcattctttt tctcttcggc
     4441 tatatccttg taatttactt ctgttggttg acgctttgtt tgttgttggt tgatattttg
     4501 cgattcttca gtaaaagttg cttcttgcgg attaatattt tgtctattat tcgtcgctgt
     4561 atcaaaagaa taattaagct gtctttcttc ttctaattga ctttctagtt tttgaatttt
     4621 tttattgctt tctaaagcta atatttgttg attctccaac aatttattta acgtattaac
     4681 attgctattt tgttgatcta attgcttagt taaattctca atatattttt catcattttt
     4741 agctctagtt tcaaagattt ctatttgctt ttctaattcg tttacttttg tttttaatgt
     4801 ctcagaacta tttgaattat tatatatatt cttttctgat tttgcagtat tttcaccaaa
     4861 caattctttt tttactgatt ctttattttg gattgtactc tctttcttag taattttttc
     4921 tataattttc aaataatcat tatcatcaat ataattaatc ccattttctt tttcaaaagt
     4981 tatatttaaa cttttagcat tattaacaat agtttgttta gttacaccta attcatcagc
     5041 aaccatttta atagttctca taacatccac ccttaggtac ctaatttagt acctaaattt
     5101 taccacctaa tgaattttat ttcatcaatt accactaaat ttactaccta tttactacat
     5161 atgcacctaa ttagctacct ataatatgtt tttggagctt aattaggtac taaaacaatg
     5221 aactacacat tttgccatgg caatgtgctt ttaactacat ttaaaactct ttatatactt
     5281 tcttaaaagt gttaatattg ctttataaat actaaaagag tcaatgctat tggcgtagca
     5341 ttgactctcg gtaataaaac gattcgcact cgtttgttta tatatttttt gatacttgta
     5401 ttatatctat ctaatcatct aagtgcaagc acaaaacata taacttacgt aaaaattgtt
     5461 ttattacctc aatcctaaaa atggaaatga ggtttttatt atgcccaatt ttgaaaaata
     5521 caatttatca caagtaaaaa ctgaaaggtt ttatcaacta cctaaatatt tattcgaaga
     5581 tgcatatttt aaaaaaatgt cggcggaagc caaaattatg tatgcgttat taaaagatcg
     5641 ctttgaatta tccatccaaa atgaatgggt agataaaaat aataacattt actttatttt
     5701 cagtaataaa catttgtgcg aatacttagg ttatgcagaa caaaaaatta taaaattaaa
     5761 aaaagagtta ataagtttta atttattaac tcaagaacgt gttggcctta ataaaccaaa
     5821 tagattatat ttattaaaac ctaattatga cattaaagcc agtcatagca aggaacttcc
     5881 aaattcacag ttccagaaca atgaatttgg aagttctaga actgtgaatt taagtggtca
     5941 agaacttcca aattcacagt ctaatgatac tgagaataat gatactgatt atattaaaac
     6001 tgagagtaat gatacgaatg atttgaatga taataagcta acttttccta gtaatcatac
     6061 aaatcattca aatcacaaca attcaaactt taataatgaa gctttaaaat tccaattact
     6121 cgaagaacta cctcaaagta ttcaaaggta tttaagtaac ttttctgtaa atgaaattaa
     6181 aatcatcaaa cctgtattat taaaagccaa gacgtccttt aataattcta ttgatacata
     6241 ttacttatta gaagatatgg aaatagaaat acttcatgtt cttaaacgtt tcaaagctat
     6301 gcttattcaa aaaaatgaaa ccgttgaagc catgcaaggg tacttaatga aatcccttaa
     6361 gtctgaattc gctgaaatgc atacgcttaa taaacgacgt gatcatttac caatcacttc
     6421 tttatttaat caataatgat tcaattagag agcatcttta aatagatgtt ctcttttttt
     6481 atttacaagt ggttgctaag gcaaccacct agatcaaacg aaaggtaagg gaggggataa
     6541 caaaataaga tcaacacaat atcacaatat agcatgctgc tattaaagaa aggaggaaga
     6601 attatgagta ctaagaaaaa gattaaagtt actttggcaa tgattaatgt tcttttaact
     6661 gcagtcgaac tttatcttca atggcaattg agtaaatctg aaaatgaact gtagttaaac
     6721 aagtaggtag gactaaaacg tttctgtttt agtcctattt tttctataaa tggttattat
     6781 tcatttgaaa atggctcaaa catgtcgcat aagatgaaaa ataaaatgat catttaacgt
     6841 tgttttaact ttaaaaatta taaaaatact aaccgttttt atattcttta gcaatctttc
     6901 ttagttataa aagtaactta ataaaatcgt ttttgtcatt tactttgtta atgatcggaa
     6961 agaaaagatt gattttatca ttccaaaaaa tcaaaattca gattcaattg atatacatca
     7021 taaacaaaaa gtaagtcgtt aatgttcaag acataagtcg aatgtaattt taaatttgat
     7081 tttttctatc ccaaactgag taaagatatt tacttagttt tctttttttt ttatctccaa
     7141 ttattttact aacaatgctt attttgaaaa taaaaataag cacttaaagt aaaaagtgaa
     7201 caagagaata gccacttttg tcttaaagca cgttttgttt ttggaaatat tacaaatata
     7261 ataggatttt caaatagtat ttgacatgag cataaggaaa tccagtacca cctttggtgg
     7321 tattcagcat agctgaagga ttgtcttacc gttcgctttg cttacttgct ctttgaaatt
     7381 cactacaagt gttgatataa caacatttat agttaatatt aaataaaaat taatcgacat
     7441 tataaacctt aatattgcat actatttaca attgttacaa gaccacttta aattgtctga
     7501 atactattgt ttttattgat ttcataggct tcagtcgtcg tcaatcctat aaataggatt
     7561 gattttttta tccaaatcct attaatccta ttacttttag tattaatatc aaaaataaaa
     7621 tcactcaaaa gtaatctaag taattcattc atttgtttta ctttttgaaa ttgactaaat
     7681 actttttgct caatatcgta ttcaataatg atcttattat ttatttttaa gttcaaaaaa
     7741 gaagggaaag aataaaacaa tttctatact tttctatgct cgattttatt aaaaagtgac
     7801 ccgaaataaa gatcacttta tctttttatt aaatagaaaa ttaaaaaaac ggaaaaatat
     7861 ataataagat tcatcgaatg attactcaaa tttttgtttg tgaaatacat ttatatttat
     7921 ttgattctca tttgcgtttt aagagctaac ttaaaatttc agtagctctt actcactttt
     7981 tggatgataa aagtgaaaat aataccaaaa aatcaagaaa agatatttta attataacgt
     8041 agcatttatg acgaaaaaat ttattgttcg ttgagaagaa ctcttaacta aacttataga
     8101 cgaatgtcgg tatagcgtga gctattaagc cgaccattcg acaattttag ggagcaattg
     8161 tgataaagta ttaatgtttt aaacctgtta catcattgat ttcgttggga tgtaacaggt
     8221 ttgtttcttt taatttggat ttgttgtgag attataaatt tataaaaaac aaggagaagc
     8281 ttatgatatc gaataaatta aaatctgcga gattagcaaa ggaattaacg caatctgagg
     8341 tagctaaaaa attatacgtt actagacaaa ctgtttctcg atgggagcaa ggtaaaacgt
     8401 tgcctaatat ttatgtaatt caagaattaa gcgatcttta tgatatttct ttagatgaat
     8461 tagtagtaaa tacagcatta gaaaataagg aggctaaaag tatgaaaaat gtaaatcctt
     8521 ttgctttatt tggagcgata ttatttaatg tgtttttatt ttctttaata gttatatttt
     8581 ttggtggctt tttgttatta tctttattca tttctttagc tatgttattt agtcctttga
     8641 ttttagcaat ctttgtttta acgggtgtac aagagtttaa tgtaattcaa ctagttatga
     8701 ctgttatttt cgttgttatt ggtttgttat caattccttt attgaaaaaa gtcagtgtat
     8761 ttttatttaa atttttcaaa cgatatttga aatttaatca taaagcaatt ttttattgaa
     8821 tttagaaacg agtttcttta taaaagtaag caaagatata atataaaatg acttatcgga
     8881 agtgaatatg cttatgtatt tacaaatgat ataataatag agtcgcctat ctctcaggcg
     8941 tcaatttgac gtagagagga ggtgcatacg cgatgtctga aatctttgtg aacatcatga
     9001 ccacagcagc aagtggttgt ctcgttgcgc tatttgcgca ttggctacgc aagcgtaatg
     9061 acgaataggc gactagtcac accaaaaaat ccccttacta cggccatagt aaggggattt
     9121 ggtgcataca tgcctgataa tctttgttaa ttacattgta acactataat tggtaggaat
     9181 gcaaagttca taaagtttga gcgtaaatat tataattacg gagttatttt atttatatct
     9241 gtagtatata taaataggaa tagggttaat gatatttata aaacatgcat ttttaattga
     9301 tgttttaata actatactga tatatgttat atattataag aatcatttga taataatgtg
     9361 agtcagtaac atttttatat ggcttagaga ggaagtttta gaatgaagaa gatgttacgt
     9421 atatcgattt tcataatagt ggtctccata tttattgttt ttaaatactc ttatcaaatt
     9481 gagggaattc tagaagaata tccaaatatt ttaaaagcat ttgtagcatt attcactctc
     9541 cttacaactg ttatcagtct tgttatcgca tataaatcat atgataatag cgttaagcaa
     9601 caagaaaaag ataaagaaga aaaaaacaaa cagttcgaag aaaatagcga taggattatt
     9661 aatagtttga aatctgattt agcaaaatat actaggaaaa taaatgtttt tagattagga
     9721 tatacgaatt caggttctgt tgcaaacttg aaaatcatat aatatagata caaagaaatg
     9781 aaaaataata aaatccaacc gaataatctg ttgatataat agtatataat ttactgcttt
     9841 tgcataactt tgtatattaa tttaactttg caacagaacc aaacgacata ttggggaaaa
     9901 tcaaatggat ttagttgaga attgtaatac atttttgtct tttgtgacga atagaagaat
     9961 ggacaaaaag aaattatata aatctaatcc atgtaaaaat cgtttttgtc cgatgtgtgc
    10021 atggcgaaag gcgagaaaag atgcattagg tttgtcattg atgatgcagc atattaagca
    10081 agcagaagat aagcaattca tctttttaac acttacaacg ccaaatgtaa caagtgaaca
    10141 tttggaaagt gaaataaaac attataatca atcattcaga cgattaagta atcgtaaaca
    10201 ttttaagtct atagctaaag gttatgttag aaaattagaa attacttata acaaaaagcg
    10261 tgatgattat aacccacatt ttcatgtatt gattgccgtt aataaatcat attttaaaga
    10321 tactaaagct tatattactc aaaaagaatg gctagaaatg tggcgtgatg tcacaggaaa
    10381 ttctgaaatt acacaagtac atatacaaaa agttaagcaa aataataata aagaacttta
    10441 cgaaatggct aaatattctg gtaaggataa tgactattta attaatcaaa aagtgttcga
    10501 cgcattttat aaagcgttaa aaggaaaaca agttcttgtt tattctggac tgtttaaaga
    10561 cgcaagaaag aaattaaaaa atggagaatt agattactta aaagaagttg atccgaccga
    10621 atatatttat caaatttttt atacttggaa taaaaaagag tatttagcga gtgaaatttt
    10681 tgatttaaca gaaagtgaaa aacaagaaat taatcatcaa atgattgatg aaattgaaga
    10741 agaaaaataa aaataaattt tcttaaacgt tagcaaaaag ctagcgtttt tttgttgaaa
    10801 atacgttaaa aatggctggg tacaaactgg gtacagacac aatatatagg ggtacaaccg
    10861 gggtacaatc gcaatttgta ggggtacaat cggggtacaa aataaatttc taggcaccta
    10921 tgaaaaatct ctgtatccct tgaaatgtct ggcttcgcca gacccacctt tttaaaaggt
    10981 ggcaaatttc ccttatgctc ttaactcttg atttgggttc aattcaaaat ttggtatttt
    11041 ttgaaagaaa ttgcccctgc aggaactaaa aaagttgatg aatttttgag ccattttatg
    11101 ccaattttta atttgcatat aactgggcac agtgtctaaa aaaacggcac tggttttgct
    11161 caaatttttg tttgtgaaat taaaatatat ttatttggct ctcatttgcg ttttaagagc
    11221 caatataagt ttttgggtat aaaactatat aaataactct aatttttcaa aataccgcct
    11281 taaaattaaa aataaaggta ttctgatttg taataaatta aaatataaaa aaagagccac
    11341 catttaaaat ttaggcagct cttactcact ttttggatga taaaagtgga aaattaattc
    11401 gagaaaaaac agaaaggata ttttaattat aacacggcat ttatgccgag aaaatttatt
    11461 gatgttgaga agaaccctta actaaacttg tagacgaatg tcggtatagc gtgagctatt
    11521 aaaccgacca ttcgacaagt tttgggattg ttaagggttc cgaggctcaa cgtcaataaa
    11581 gcaattggaa taaagcaatt atgttattga ttttaaattt tttatatcaa aatggtcttt
    11641 ttctgttaaa tcataaccag aatgaaaaag aagttgagct tcttttgaaa tacatggtat
    11701 ttttcgacct ttgtaattag tttctgaaaa ccagtcattt tggaaaacat aattaccacc
    11761 ttctgggttt gcttgggtaa ttgatccatc atcatttaga tttataggat gaatatctaa
    11821 atacccatat tcttcatgct taagttccat acgtgaaggc atccaatcaa cttctatttt
    11881 gtatcctata tcttctaatt tttgtataac tttttgagtg tgttgagcgt caaaatctat
    11941 atctatatct ctgtgttctc tttgttgttt tccagttaat acatctaccc cccagccacc
    12001 atctaaccaa taagttactt tcatgtgttc aaataaatct aaaatataaa ataaatcttt
    12061 ttctgttaca ttattatttt tcattttatc cctcctaact tgggaatttg aaacaagttg
    12121 aaaacgagtt tcttcttgtc ttgataataa gggtaactat tacaagctgt gatagttacc
    12181 cttattccat tgatatgact ggttttaagc acaaaaaaag ttgccttttc cagcatacta
    12241 ttgtatcgtt ttaggtgtct aagctaaacg aaaaatagaa aggagaaaaa gcaacgtctt
    12301 ttcgtatttc aaacaaagtt gttttatgtg aattatatga aaaagtatac tgagaaaaaa
    12361 caaagaaatc aagtatttca aaactttatt aaacgacagg ttctgttgca aacttgaaaa
    12421 tcatataata tagatacaaa gaaatgaaaa ataataaaat ccaaccgaat aatctgttga
    12481 tataatagta tataatttac tgcttttgca taactttgta tattaattta actttgcaac
    12541 agaaccaaaa aattaataaa aatcttgttg gttttatgcg tgcaacggaa gtgacaataa
    12601 ataataaaga taattcttat aatcagcaca tgcatgtatt ggtatgtgtg gaaccaactt
    12661 attttaagaa tacagaaaac tacgtgaatc aaaaacaatg gattcaattt tggaaaaagg
    12721 caatgaaatt agactatgat ccaaatgtaa aagttcaaat gattcgaccg aaaaataaat
    12781 ataaatcgga tatacaatcg gcaattgacg aaactgcaaa atatcctgta aaggatacgg
    12841 attttatgac cgatgatgaa gaaaagaatt tgaaacgttt gtctgatttg gaggaaggtt
    12901 tacaccgtaa aaggttaatc tcctatggtg gtttgttaaa agaaatacat aaaaaattaa
    12961 accttgatga cacagaagaa ggcgatttga ttcatacaga tgatgacgaa aaagccgatg
    13021 aagatggatt ttctattatt gcaatgtgga attgggaacg gaaaaattat tttattaaag
    13081 agtagttcaa caaacgggcc agtttgttga agattagatg ctataattgt tattaaaagg
    13141 attgaaggat gcttaggaag acgagttatt aatagctgaa taagaacggt gctctccaaa
    13201 tattcttatt tagaaaagca aatctaaaat tatctgaaaa gggaatgaga atagtgaatg
    13261 gaccaataat aatgactaga gaagaaagaa tgaagattgt tcatgaaatt aaggaacgaa
    13321 tattggataa atatggggat gatgttaagg ctattggtgt ttatggctct cttggtcgtc
    13381 agactgatgg gccctattcg gatattgaga tgatgtgtgt catgtcaaca gaggaagcag
    13441 agttcagcca tgaatggaca accggtgagt ggaaggtgga agtgaatttt gatagcgaag
    13501 agattctact agattatgca tctcaggtgg aatcagattg gccgcttaca catggtcaat
    13561 ttttctctat tttgccgatt tatgattcag gtggatactt agagaaagtg tatcaaactg
    13621 ctaaatcggt agaagcccaa acgttccacg atgcgatttg tgcccttatc gtagaagagc
    13681 tgtttgaata tgcaggcaaa tggcgtaata ttcgtgtgca aggaccgaca acatttctac
    13741 catccttgac tgtacaggta gcaatggcag gtgccatgtt gattggtctg catcatcgca
    13801 tctgttatac gacgagcgct tcggtcttaa ctgaagcagt taagcaatca gatcttcctt
    13861 caggttatga ccatctgtgc cagttcgtaa tgtctggtca actttccgac tctgagaaac
    13921 ttctggaatc gctagagaat ttctggaatg ggattcagga gtggacagaa cgacacggat
    13981 atatagtgga tgtgtcaaaa cgcataccat tttgaacgat gacctctaat aattgttaat
    14041 catgttggtt acgtatttat taacttctcc tagtattagt aattatcatg gctgtcatgg
    14101 cgcattaacg gaataaaggg tgtgcttaaa tcgggccatt ttgcgtaata agaaaaagga
    14161 ttaattatga gcgaattgaa ttaataataa ggtaatagat ttacattaga aaatgaaagg
    14221 ggattttatg cgtgagaagg ttctgttgca aagttgaatt tataatataa ttttaacaaa
    14281 aaggagtctt ctgtatgaac catttcagat ataaacaatt taacaaggat gttatcactg
    14341 tagccgttgg ctactatcta agatatgcat tgagctatcg tgatatatct gaaatattaa
    14401 gagaacgtgg tgtaaacgtt catcattcaa cgatctaccg ttgggttcaa gaatatgccc
    14461 caattttata tcaaatttgg aagaaaaagc ataaaaaagc ttattacaaa tggcgtattg
    14521 atgagacgta tatcaaaata aaaggaaaat ggagctattt atatcgtgcc attgatacag
    14581 agggacatac attagatatt tggttgcgta agcaacgaga taatcattca gcatatgcgt
    14641 ttatcaaacg tctcattaaa caatttggta agcctcaaaa ggtagttaca gatcaggcac
    14701 cttcaacgaa ggcagcaatg gctaaaataa ttaaagcttt taaacttaaa cctgactgtc
    14761 attgtacatc gaaatatctg aataacctca ttgagcaaga tcaccgtcat attaaagtaa
    14821 gaaagacaag atatcaaagt atcaatacgg caaagaatac tttaaaaggt attgaatgta
    14881 tttacgctct atataaaaag aaccgtaggt ctcttcagat ctacggattt tccccatgcc
    14941 acgaaattag catcatgcta gcaagttaaa cgaacactga catgatatat tagtgattag
    15001 ctatattttt tactttgcaa cagaaccatc acgatttaga caatttttct aaaaccggct
    15061 actctaatag ccggttggac gcacatactg tgtgcatatc tgatccaaaa ttaagttttg
    15121 atgcaatgac gatcgttgga aatctcaacc gagacaacgc tcaagccctt tctaaattta
    15181 tgagtgtaga gccccaaata agactttggg atattcttca aacaaagttt aaagctaaag
    15241 cacttcaaga aaaagtttat attgaatatg acaaagtgaa agcagatagt tgggatagac
    15301 gtaatatgcg tattgaattt aatccaaaca aacttacacg agatgaaatg atttggttaa
    15361 aacaaaatat aataagctac atggaagatg acggttttac aagattagat ttagcctttg
    15421 attttgaaga tgatttgagt gactactatg caatgtctga taaagcagtt aagaaaacta
    15481 ttttttatgg tcgtaatggt aagccagaaa caaaatattt tggcgtgaga gatagtaata
    15541 gatttattag aatttataat aaaaagcaag aacgtaaaga taatgcagat gctgaagtta
    15601 tgtctgaaca tttatggcgt gtagaaatcg aacttaaaag agatatggtg gattactgga
    15661 atgattgctt tagtgattta catatcttgc aaccagattg gaaaactatc caacgcactg
    15721 cggatagagc aatagttttt atgttattga gtgatgaaga agaatgggga aagcttcaca
    15781 gaaattctag aacaaaatat aagaatttga taaaagaaat ttcgccagtc gatttaacgg
    15841 acttaatgaa atcgacttta aaagcgaacg aaaaacaatt gcaaaaacaa atcgattttt
    15901 ggcaacatga atttaaattt tggaaatagt gtacatatta atattactga acaaaaatga
    15961 tatatttaaa ctattctaat ttaggaggat ttttttatga agtgtctatt taaaaatttg
    16021 gggaatttat atgaggtgaa agaataattt acccctataa actttagtca cctcaagtaa
    16081 agaggtaaaa ttgtttagtt tatataaaaa atttaaaggt ttgttttata gcgttttatt
    16141 ttggctttgt attctttcat tttttagtgt attaaatgaa atggttttaa atgtttcttt
    16201 acctgatatt gcaaatcatt ttaatactac tcctggaatt acaaactggg taaacactgc
    16261 atatatgtta actttttcga taggaacagc agtatatgga aaattatctg attatataaa
    16321 tataaaaaaa ttgttaatta ttggtattag tttgagctgt cttggttcat tgattgcttt
    16381 tattggtcac aatcactttt ttattttgat ttttggtagg ttagtacaag gagtaggatc
    16441 tgctgcattc ccttcactga ttatggtggt tgtagctaga aatattacaa gaaaaaaaca
    16501 aggcaaagcc tttggtttta taggatcaat tgtagcttta ggtgaagggt taggtccttc
    16561 aataggggga ataatagcac attatattca ttggtcttac ctacttatac ttcctatgat
    16621 tacaatagta actatacctt ttcttattaa agtaatggta cctggtaaat caacaaaaaa
    16681 tacattagat atcgtaggta ttgttttaat gtctataagt attatatgtt ttatgttatt
    16741 tacgacaaat tataattgga cttttttaat actcttcaca atcttttttg tgatttttat
    16801 taaacatatt tcaagagttt ctaacccttt tattaatcct aaactaggga aaaacattcc
    16861 gtttatgctt ggtttgtttt ctggtgggct aatattttct atagtagctg gttttatatc
    16921 aatggtgcct tatatgatga aaactattta tcatgtaaat gtagcgacaa taggtaatag
    16981 tgttattttt cctggaacca tgagtgttat tgtttttggt tattttggtg gttttttagt
    17041 ggatagaaaa ggatcattat ttgtttttat tttaggatca ttgtctatct ctataagttt
    17101 tttaactatt gcattttttg ttgagtttag tatgtggttg actactttta tgtttatatt
    17161 tgttatgggc ggattatctt ttactaaaac agttatatca aaaatagtat caagtagtct
    17221 ttctgaagaa gaagttgctt ctggaatgag tttgctaaat ttcacaagtt ttttatcaga
    17281 gggaacaggt atagcaattg taggaggttt attgtcacta caattgatta atcgtaaact
    17341 agttctggaa tttataaatt attcttctgg agtgtatagt aatattcttg tagccatggc
    17401 tatccttatt attttatgtt gtcttttgac gattattgta tttaaacgtt ctgaaaagca
    17461 gtttgaatag ttatattata ttttggttta gaactatgag tggctagcat tttgccactc
    17521 attttttgcg ttagcaaaaa caggtttaag cctcgcagag cacacgtatt aacgacttat
    17581 taaaaataag tctagtgtgt tagacttaaa ctattaaata cacatgaaac ctttgtgctt
    17641 aggagtgatt tttatatgtc ttattccatt gttagagttt caaaagttaa atctggaaca
    17701 aatacaacgg gcatacaaaa acatgttcaa agagaaaata ataattatga aaatgaagat
    17761 atagaccata gtaaaactta cttaaattat gatttggtaa atgctaataa acagaatttt
    17821 aataacttga ttgatgaaaa aatcgaacag aattatacag gcaaaagaaa aattagaaca
    17881 gacgcgatta aacacattga tggtttaatt acatcagaca atgatttctt tgataatcaa
    17941 acgccagaag atacaaagca gttttttgaa tatgctaaag agtttttaga acaagaatac
    18001 ggtaaagata atttattata tgcaacagtt cacatggacg aaaaaacacc acatatgcat
    18061 tatggcgttg ttccaataac tgatgatggt cgtttaagtg ctaaagaagt tgtaggtaat
    18121 aaaaaagctt taacagcgtt tcaagataga tttaatgagc atgttaaaca acgaggatat
    18181 gatttagaac gtgggcaatc aagacaagta acaaatgcta aacatgagca aataagtcag
    18241 tataaacaaa aaaacagaat atcataagca agaatatgaa cgtgagagcc aaaaaacaga
    18301 ccatataaag caaaagaacg ataaattaat gcaagagtac caaaaatcgt taaatacgct
    18361 taaaaagcct ataaatgttc cgtatgagca agaaactgaa aaagtaggtg gtttatttag
    18421 caaagaaata caagaaactg gaaatgttgt aataagccaa aaagatttca atgaatttca
    18481 gaaacagata aaagctgctc aagatatttc ggaagattac gagtatataa agtctggtag
    18541 agccttagat gataaagata aggaaatacg agagaaagat gatttattaa ataaagcagt
    18601 tgagcgtatt gaaaacgcag acgataattt taaccaactt tacgaaaatg caaagccact
    18661 taaagagaat atagaaatag cgttaaagct tttaaaaatc ttactaaaag agttagaacg
    18721 agttttagga agaaatacct ttgcggaaag agttaataag ttaacagaag atgaaccaaa
    18781 actaaatggt ttagcaggaa acttagataa aaaaatgaat ccagaattag gttctgttgc
    18841 aaagttgaat ttataatata attttaacaa aaaggagtct tctgtatgaa ctatttcaga
    18901 tataaacaat ttaacaagga tgttatcact gtagccgttg gctactatct aagatatgca
    18961 ttgagctat
//
