LOCUS       NZ_CP151142             3122 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus aureus strain BSN47-2 plasmid pSAP046B, complete
            sequence.
ACCESSION   NZ_CP151142
VERSION     NZ_CP151142.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637637
            Assembly: GCF_038096205.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN47 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN47
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly :: CP043386, CP014445, CP003981, GQ900404
            Genome Coverage                   :: 126.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096205.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:48:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,991
            CDSs (total)                      :: 2,913
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 42 of 83
            Pseudo Genes (incomplete)         :: 43 of 83
            Pseudo Genes (internal stop)      :: 18 of 83
            Pseudo Genes (multiple problems)  :: 19 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151142.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN47-2"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pSAP046B"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2020-10-12"
                     /collected_by="Andrew D. Berti"
     gene            complement(440..1435)
                     /locus_tag="UXQ83_RS14780"
                     /old_locus_tag="UXQ83_14780"
     CDS             complement(440..1435)
                     /locus_tag="UXQ83_RS14780"
                     /old_locus_tag="UXQ83_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_341532640.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1887..2468
                     /locus_tag="UXQ83_RS14785"
                     /old_locus_tag="UXQ83_14785"
     CDS             1887..2468
                     /locus_tag="UXQ83_RS14785"
                     /old_locus_tag="UXQ83_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(2791..2979)
                     /locus_tag="UXQ83_RS14790"
                     /old_locus_tag="UXQ83_14790"
     CDS             complement(2791..2979)
                     /locus_tag="UXQ83_RS14790"
                     /old_locus_tag="UXQ83_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          complement(206..370)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          complement(441..1435)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.699"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacgcaaata tgagccaaat aaatatatgg taatttcaca aacaaaaatt tcagcaaaac
       61 cagtgactaa ttttttaaca ctgcccattt acatgcaaat taaaaattag cataaaatcc
      121 tttaaaactt ccacatgttt ttctaaattc ctgcagggcg ttttttatca aaaatcgaga
      181 atttttaatt tttctctaaa aactccgtaa gagcataagg agaatttgct atcattcaaa
      241 aatgataggt ctggcaaagc cagaaattcc aggggtttta aggtatttta aagtgatatc
      301 atacaaagct gtactgtctt atttttgtga cacaatatat tgcgtttgtc ttatttttgt
      361 gacactacat acagcatttg tcacaaaaat aagacagtac ttttttataa aaaatcaaca
      421 aaaaagacat tagtcaacac taatgtctta aataataatt ttgcttttcg aaattccaaa
      481 ttgcggtaat tgaatgtgct ttttcttctt cgtctgttgt tttatcttca tcacttgcat
      541 taatcaaatt accatcttcg gcatcgtcta agtttaaaat tttatgcttt tgtttaagca
      601 atccaccata acttaacata cgttttcgat acaaaccttt ttctaaatca cttacaattt
      661 cttgattttt ttcatcatca tcagttaaaa aatcagatga tttaaccgaa tattttgagg
      721 tctctttgat tgccgattca atgtctttat cgccttttct attcggtttg atcgctttaa
      781 cattagcaac aggtcgatag tcaacttgta atgctctttg ccataaatta acccattctt
      841 cttgagttat ataattctct ttttttctaa aatatgcatt ttcaacacat aacaaaacat
      901 gcatgtgctg attataacta ccgtcatttt tattaacggt aacttctgtt gaacgcataa
      961 atccaacaag attttgttta acctttttat atctactcaa cctatcaaat gctttagtta
     1021 gatgcttcaa actttgttct aaagtatctc catctatcgc attttttgtt gaaagtgtta
     1081 aaaacaacca acgtgctttt ggcttttcct taattacttt ttcaatcact ttttgagctt
     1141 gataactatt tttcatagca cgtctccaat tacaaaccgg acataatttt gatttacaaa
     1201 accatgtctt atgtaacttc aaatagcctt catcagttgg cttaaattct aaaatattac
     1261 cacattgttt tacattaaaa gcctttttga tttttaaaat ttctagtata tctgcatagc
     1321 ttacattatc tattttcttt tctctccatg ggcgttgttt cccacttttc gtcatatctt
     1381 ttaacgtttt attatcttga ttttcggtta tacttctagt agtattatat tgcatataaa
     1441 aagaccccca gtattattta agattcgaca ccttaataat ataactgggg ttttttattg
     1501 tcaaattttc ctttcacgca caaaaacacg tttaaacact gtaatatcaa cagttacagc
     1561 tatatatata aaatcgagtt atttctatat agtatcaaga caagaagaaa ctcgttcaca
     1621 ctcgtttcaa aaaacaacct attccttctt ctttttatga ccctctaaac ctaaataaat
     1681 taatgtgaaa accaatgaat aaataaaact ttctaaaaag tcaaaaggat tatcaaataa
     1741 aatcgaatta acaaaattaa aaattaaaaa tacagataat aaaataatca aaaagtaaaa
     1801 aaacttattc atattaaact cctttgtttt tatatgttaa tattatattg aattcattta
     1861 aataacaaaa caagggggca tttacaatga atgcaattca aaacagtata tcactaagaa
     1921 agattttagt agtatcttta agtattttaa ccatactttc aatggtcttg gattttaatt
     1981 tcaaagaagc tcaagcccaa aacaaaaata acattgcaga taaaaacgtt gaaactttaa
     2041 acgaaaaaga aattgaaaaa gaactaaaat acatatatgg caaaattatt attttagata
     2101 aagacggaac agctaaagat gttaatctag aaaatgccaa atctcgatat ggatatgttc
     2161 ctgaaggatt ccaaaaacta aaaaacgaca tagagaataa aaagaaaact agttcaatat
     2221 cacctagagc agttggtggg aactacaaaa atagcggtga ctgtttctat tctgaagttt
     2281 taaactcata tggagaactt ttaactggaa acataatcac agcagttttt gaagatgtta
     2341 aagccaaaaa cttaaagggc gtagcaaaaa aattagctaa aattgggata aaaggaaacc
     2401 tagctggaat cgcagctact atggtttcga aggatgtaca atgtaatctt aaatacgggt
     2461 tactttagat actatttatt ccaattgctt tattgatgtt gagaagaacc cttaacaatc
     2521 ccaaaacttg tcgaatggtc ggcttaatag ctcacgctat gccgacattc gtctgcaagt
     2581 ttagttaagg gttcttctca acatcaataa attttctcgg cataaatgcg tcgtctattt
     2641 tttattatct tcatgataat catatttttc ttccatctcg tcaaaagcat catctttata
     2701 gaaaaacaaa aagtaagcaa caacaccaat aggaataaaa aaacagtgtg aaaacaagaa
     2761 gaaactcgtt tcaaaatccc ctcaaatatt ctaattattc tttttgaaat tcataacctt
     2821 aacaaccaaa taaatagcat ttattcccat caaaataaca tacggaatta atatatataa
     2881 attttccaaa tgagatataa aaattaatcc aagtaaaaag aaaacattta aaatagttgc
     2941 tataacgtaa ccttcatcta acactgtgtt tttattcaaa ataaacactc cttttttaaa
     3001 aaactttatc acaagactta ctcaaattat aaataccttt attttgagtt ttaagggggt
     3061 attttaaagg tttagggggt atcatatagg tttttaactt aaaaatatat acaagctctt
     3121 aa
//
