LOCUS       NZ_CP151126            13364 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus aureus strain BSN154 plasmid pAH5667, complete
            sequence.
ACCESSION   NZ_CP151126
VERSION     NZ_CP151126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637733
            Assembly: GCF_038096265.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN154 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13364)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN154
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP049416, CP092053
            Genome Coverage                   :: 155.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096265.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 22:35:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,786
            CDSs (total)                      :: 2,705
            Genes (coding)                    :: 2,627
            CDSs (with protein)               :: 2,627
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 45 of 78
            Pseudo Genes (incomplete)         :: 34 of 78
            Pseudo Genes (internal stop)      :: 15 of 78
            Pseudo Genes (multiple problems)  :: 15 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151126.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13364
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN154"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pAH5667"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-11-09"
                     /collected_by="Andrew D. Berti"
     gene            join(13344..13364,1..963)
                     /locus_tag="UXQ14_RS13860"
                     /old_locus_tag="UXQ14_13860"
     CDS             join(13344..13364,1..963)
                     /locus_tag="UXQ14_RS13860"
                     /old_locus_tag="UXQ14_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181102.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRFSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            <983..1272
                     /locus_tag="UXQ14_RS13865"
                     /old_locus_tag="UXQ14_13865"
                     /pseudo=""
     CDS             <983..1272
                     /locus_tag="UXQ14_RS13865"
                     /old_locus_tag="UXQ14_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            1817..1966
                     /locus_tag="UXQ14_RS13870"
                     /old_locus_tag="UXQ14_13870"
     CDS             1817..1966
                     /locus_tag="UXQ14_RS13870"
                     /old_locus_tag="UXQ14_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(2128..2820)
                     /locus_tag="UXQ14_RS13875"
                     /old_locus_tag="UXQ14_13875"
     CDS             complement(2128..2820)
                     /locus_tag="UXQ14_RS13875"
                     /old_locus_tag="UXQ14_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            2930..3124
                     /locus_tag="UXQ14_RS13880"
                     /old_locus_tag="UXQ14_13880"
     CDS             2930..3124
                     /locus_tag="UXQ14_RS13880"
                     /old_locus_tag="UXQ14_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            4241..4858
                     /gene="cadD"
                     /locus_tag="UXQ14_RS13885"
                     /old_locus_tag="UXQ14_13885"
     ARG             4242..4855
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4877..5224
                     /locus_tag="UXQ14_RS13890"
                     /old_locus_tag="UXQ14_13890"
     CDS             4877..5224
                     /locus_tag="UXQ14_RS13890"
                     /old_locus_tag="UXQ14_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            5381..5473
                     /locus_tag="UXQ14_RS13895"
                     /old_locus_tag="UXQ14_13895"
     CDS             5381..5473
                     /locus_tag="UXQ14_RS13895"
                     /old_locus_tag="UXQ14_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5645..6616)
                     /locus_tag="UXQ14_RS13900"
                     /old_locus_tag="UXQ14_13900"
     CDS             complement(5645..6616)
                     /locus_tag="UXQ14_RS13900"
                     /old_locus_tag="UXQ14_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            6740..7159
                     /locus_tag="UXQ14_RS13905"
                     /old_locus_tag="UXQ14_13905"
     CDS             6740..7159
                     /locus_tag="UXQ14_RS13905"
                     /old_locus_tag="UXQ14_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(7685..8530)
                     /gene="blaZ"
                     /locus_tag="UXQ14_RS13910"
                     /old_locus_tag="UXQ14_13910"
     ARG             complement(7689..8530)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8637..10394
                     /gene="blaR1"
                     /locus_tag="UXQ14_RS13915"
                     /old_locus_tag="UXQ14_13915"
     ARG             8638..10391
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            10384..10764
                     /gene="blaI"
                     /locus_tag="UXQ14_RS13920"
                     /old_locus_tag="UXQ14_13920"
     ARG             10385..10761
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            11028..11606
                     /locus_tag="UXQ14_RS13925"
                     /old_locus_tag="UXQ14_13925"
     CDS             11028..11606
                     /locus_tag="UXQ14_RS13925"
                     /old_locus_tag="UXQ14_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(11967..12710)
                     /locus_tag="UXQ14_RS13930"
                     /old_locus_tag="UXQ14_13930"
     CDS             complement(11967..12710)
                     /locus_tag="UXQ14_RS13930"
                     /old_locus_tag="UXQ14_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             complement(7686..8530)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          1328..1475
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.644"
                     /coverage="100.0"
                     /evalue="1.3e-63"
     Typing          2..963
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.896"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          1095..1264
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
     Typing          complement(11968..12710)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcaagaaa attacaaaga aagattttat caaatcccta aagtattctt taccagtgaa
       61 aattataaaa atctaactaa tgatatgaaa atagcctatg ctatcttacg tgatcgctta
      121 aatttatcta ttaaaaatag ctgggttgat gaagatggta acatttactt tgtttattca
      181 aatgaaaagc taatggaaat cttaaattgt aaaaaagaaa agttaacaaa aataaaaaaa
      241 gggttagaaa acgatggtct actcattcaa aaacgacgtg gtttaaataa accaaatatt
      301 ctatatttaa tgaaacctat tgtcacagaa cgtgatattt acaaaataga aaaagaagaa
      361 aacgatgttg aaccttacgg cgagaaggaa gttcgatttt cgaacgtcca gaagttcgaa
      421 aatcgaacgt ccagaagttc gaaaatcgaa cgtccagaag ttcgaaaatc gaacactaat
      481 gatactgact ttaatgacat tgactttaat gatactgaga ataatgatat gaatgacttg
      541 aatgatataa agtacaaaaa tgaaattagt aatcattcaa atcattcaaa tcaatttaca
      601 cataattttg atgataaaga aatgctattg caagaattcc cagaacaact aactaattat
      661 ttattaaaat atgattatag agatttagaa atcataaagg ctgttattct taaagcaaaa
      721 aaatcattta attcaaggca tgaagatatg cattacatgt tagaggacat tgaggatgaa
      781 atattaactt ctttaaaaag acttaaaaaa gctatacatg ataggggagt taaaggccaa
      841 aaggaaacaa tcaagtcaat gcaagcttat ttaatgcaaa caattctaac tgaacttgaa
      901 gaaacacatg ccctttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa
      961 taattcaaat gattttataa cactcgaaga gacatcaaaa tattccgtta aatcatctga
     1021 ctttttaact gatgacgacg aaaaaaatca agaaattgta agtgatttag aaaaaggttt
     1081 gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa caaaagcata aaattttaaa
     1141 cttagacgat gccgaagatg gtaatttgat taatacaagt gatgaagata aaacgacaga
     1201 cgaagaaaaa aagcacattc aattaccgca atttggaatt tcgaaaagca aaattattat
     1261 ttaagacatt agtgttgact aatgtctttt ttgttgattt tttataaaaa agtactgtct
     1321 tatttttgtg acaaatgctg tatgtagtgt cacaaaagtg tgacactaca gctttgtatg
     1381 atatcacttt aaaatacctt aaaacccctg gaatttctgg ctttgccaga cctatcattt
     1441 ttgaatgata gcaaattctc cttatgctct tacggagttt ttagagaaaa attaaaaatt
     1501 ctcgattttt gataaaaaac gccctgcagg aatttagaaa aacatgtgga agttttaaag
     1561 gattttatgc taatttttaa tttgcatgta aatgggcagt atctaaaaaa ttagtcactg
     1621 attttgctga aatttttgtt tgtgaaatta gcatatattt atttggctca tatttgcgtt
     1681 ttaagagact gtatagattt ttaagtacaa aacctatatg atttgcctct aagtcttcaa
     1741 aatgcctctt aaaatccaaa ataaaagtgt ttaatattgt aataaggatt gctattttta
     1801 taaaaggaga gatatcatga agattaaaac tttcgctact tttactcatc gtcaattaga
     1861 taaacaagtt aataggtttt taaagaatga tattcatgta ttagaaattc aaacacacag
     1921 taattggtta tatatttcag ctattgtttt ttatgaggat aaataaggaa catcgtattt
     1981 atgccgaaaa aatttattga tgttgagaag aacccttaac taaacttgca gacgaatggt
     2041 cggcttaata gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc
     2101 aacatcaata aaacaattgg aataaaatca acctacagag catccagctg caaatgcagt
     2161 tccgcaaact gcttcacata caccgccggc tattggattt acgattgccc atactccaca
     2221 gtagtgagca catgcataaa gtccagccat actacaagcg aatgctttgc cgttccattt
     2281 gaatcctgca tatgttaccg gtctgttttt atcaactttt tcagccttat attcaacgta
     2341 atttttgtta ataagtattt ttccctcttt gttttcagtc atacttcttt tttcggcaat
     2401 gaattttgtg atttttttgt tgtagttatc atatattgtt tcagaaacta caatttcttt
     2461 tgttttttca tttttataag cgttattagc tatgtagcca tatgcatttt tatcatttgc
     2521 ttctgtagct agatatttta atttagaatt tttgtcttct agctttattt ttttaaatcc
     2581 ttcttttttc atttgctttt cgaatttttg catatcttca tttttatttt gcaattgttc
     2641 tagaaaactt atttctttct tggtcatttg tgatacttcg atgtttttgc ttttcaattt
     2701 ttctttttga ttttctagta aagaactttt ttgttcttca tttgcgtaag cattagaaaa
     2761 attcgcgcta aataccagta ggaacaagga aattattgaa aataatttta taattttcat
     2821 ttgatacctc caagataata agatatttta attgtataag taagcgcttt ataaaatcaa
     2881 tatttatttt aatatattta taaacatatt tttgaaagga gtctatttta tgaatgaaaa
     2941 tttagaaaaa tatataaaaa gtttgccact tttaggtttg ttaattagta ttttgttaat
     3001 tgttttattc ttttttattt tagatgttga tgaagattta ttcgtggttt ttttatactg
     3061 tttattgcct ttgatagtta atttgtctat ttatggtttt tatgtttttg ttaaaagaaa
     3121 gtaagattat gaataatttt gtttttaatt tgtatgcttt gagggggttg gaaaacggta
     3181 ggtgtataga ttagatttct tgtttttttg aaacgagtgt gaacgagttt cttcttgtct
     3241 tgatttttga taaaaacgcc ctacaggaat ttagaaaaac gtggggaagt tttaaagaat
     3301 tttatgctaa tttttaattt gcatgtaaat gggcagtgtc taaaaatagt tattgttttt
     3361 gctcaaattt ttgtttttga aaatagcata tatttatttg gctcatattt gcgttttaaa
     3421 agcatatata agttttagat ataaaactat atgaatcacc cctaaatctt taaaattccc
     3481 tcttaaaatt caaaataagg gcataaaaaa taaaagctac cccagataaa gtgaggtagc
     3541 tttttctttc atacggtcta tttatttttc tttcgacgcc gtatcgtcga tgtaattcaa
     3601 aaaagttatg ggctataaat ctacatcact ttttcaagaa tgtagtagca atattcaacg
     3661 caaattaatt tttataactg ctcggaatat ctcaagccgt ttctttaatt ttgaaataaa
     3721 aaaatcgacg aaggtcgatt acgtttttgc acatgtcgat ttatccgacg tataagtgcg
     3781 cccttacggg atttaactag attataacga cgaattttag acctgtaaag caataacaat
     3841 taggtctacc aggtagataa agtgtacaaa gtagaaccga aaaagttatt tattttgaag
     3901 atttttcgta gatataattt gctaataaac ttcctaaaac tgaataaaga aaaatagtta
     3961 aactcatgta atcactccat aattctgaag tgtctccatg tctacgcata gacgacgcat
     4021 ttatgccgaa aaaatttatt gatgttgaga agaaccctta actaaacttg cagacgaatg
     4081 tcggcatagc gtgagctatt aagccgacca ttcgacaagt tttgggattg ttaagggttc
     4141 cgaggctcaa cgtcaataaa gcaattggaa taaagcacct atgattattt aaaaatataa
     4201 gttataatac attcaaaaat atttttgaat gaggtgtatt atgattcaaa cggttgttgc
     4261 tgctgctgtt ctttatattg ctacagcagt tgatttatta gtaattttat taatattttt
     4321 tgctagagca aagactagaa aagaatatag agatatttat gttggtcaat atttagggtc
     4381 tattatttta atattagtta gtttgttttt agcttttgta ttaaattatg ttccagaaaa
     4441 gtggatatta ggtttattgg gtttaatacc aatttatctt ggaattaaag tggctattta
     4501 tgatgattgt gaaggagaaa agagagctaa aaaagaattg aatgaaaaag gattgtctaa
     4561 attagttggt acggttgcaa ttgttacgat agcaagttgt ggcgccgata atattggttt
     4621 atttgttccg tattttgtga cattaagtgt tactaattta ttacttactc tgtttgtctt
     4681 tttaatttta attttcttct tggtgtttac tgcacaaaaa ttagctaata ttccaggaat
     4741 tggagaaatt gttgagaaat ttagtcgttg gattatggct attatttata tagctttagg
     4801 tttatttatt attattgaaa atgacactat tcaaacaatt ttaggattta tattttaatt
     4861 tagggtgtga ttttatatga gttatgaaaa tacttgtgat gtgatctgtg tacatgagga
     4921 taaagttaac aatgctttaa gttttttaga agatgataaa tctaagaaat tacttaacat
     4981 tttagaaaaa atttgtgatg agaagaaatt gaaaatcata ttatctttga ttaaagaaga
     5041 tgagttgtgt gtttgtgata tttctttgat attgaaaatg agtgttgctt caacttcaca
     5101 tcatttaagg cttttatata aaaatgaggt acttgatttt tataaagatg gaaagatggc
     5161 atattatttt attaaagacg atgaaataag agagtttttc tctaaaaatc atgagggttt
     5221 ttgaaacgag tttcttcttg tcttgatact ataaaaaaca aagttatata acataagacg
     5281 acttatcgga agtagatatg cttgtatatt cacagatggt ataataatag agtcgcctat
     5341 ctctcaggcg tcaattgacg aagagaggag gtgcattact ttgctaatca ttttcgttca
     5401 catcatagca ccagtcatca gtggctgtgc tgttgcgtat tatacttatt ggcttagtaa
     5461 acgcaacaaa taattaatag gcgactttat aagccagcat cgaaaaaatc cccacactac
     5521 tgcaatagtg tggggattgg tgcattaatt gttaatcatt ttcgttatat aaattataac
     5581 actggttagt agagaaatgc aaaatgattg aattaatagt aaatgtatta atgaatactc
     5641 acacttatac ttttaaaata gttcttccta aatgttgtcc ttttaaaagc tgatctatcg
     5701 ttttatagaa ttcttcaaat ccaatttcat tgtatagaag tgaatttcct atattccatt
     5761 catttgctaa cttactccat atatcttcac ggatattatg atcaacatat acagaatcaa
     5821 tgccaattaa tttaatacct ctaagaataa acggtaatat attagcgtta agtgaatttc
     5881 ctgctacatt tccacataca gtagcaacac cttgataatt tagacgtttt aaaccgtata
     5941 atgttacatt actccccact gtatctaaga aataatcaaa aatacctttt ttaagcggtt
     6001 tattatcatc ttcaattatt attacttgtt ttgcacctaa tttttttgcg atttctattt
     6061 gcttttcttt tcttacgata gcagtgatat tttcatatcc aattttatgt aaaatttgta
     6121 tagcaacact gcccacacca cctgtagccc cagacactaa aatttcaggt tgtgatttga
     6181 tattcattcc atatttttct agttcaaaaa ttgataatgc tgccgtaaaa ccggctgtgc
     6241 catataccat agactcttta atagacattt cttggggaat aggaacaatc cactctccag
     6301 gcacttgtgc atattctgaa aatccacctg tatgtgtcac gccaacatca taaccagtaa
     6361 ctaatacatt atcgccttct ttaaacttat cattcgaact tgattcaaca atacctgcaa
     6421 aatctatacc aggaatcata ggataatctc taattacacc gcctttatat tgtaaagcta
     6481 gcatatcctt ataatttatt gaagagtata ccacttttac taatacatca cccttcgata
     6541 aatgagattc atcaactttt tctactttat aactaacatt tgatgcactt tttctcacta
     6601 ctaccgcatt gaacatttat tagccttctt tcacaattgt tttgtagaag tatgtttttg
     6661 ttgcaaaact attttcctac aaaacttatc atgatatgat aagcgagtca atactaatga
     6721 tttaagagag gttttagttg tggaattaga taattgtata aattatttgt tgagtacttc
     6781 tcagaataga gtttttaaac attttaaaag tttattaagt ccctataata ttactccagc
     6841 agaatatggt gttttgaatt gcttgattaa tagtaatttg aatacaccta aagcgattgg
     6901 taatacttta aatttagaac cttcaacaat atctggaata ttagataaga tgtccaaaaa
     6961 ggatctaata acacgtcaaa ctgataatga aaatagaaga actgtgctaa taaacagtac
     7021 aaataaagca aaagacttat ggccaacatt agaaaagaaa gcctatcaac ttaataatca
     7081 ttacttcgct aatttaacta atgaagaaac tgtagtgttt aaaaagatat tactaaagat
     7141 aaataaaact acgttttaat ttttacgtta taaaaaagtt gaagtgcgaa ttataatcga
     7201 gtaaatataa atgatagctt agcttaatta tatattaaag ccaactatac ttttattccc
     7261 gaaaaaccta ctatccaaat ttctaagata ctttagcctc tataataaac atattgtaat
     7321 ataatttatg tattatttag gtttacccta atcgaacaat aaaaaatact tgtaaaatgc
     7381 tattgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga aaaaagcaac
     7441 gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt aaactattca
     7501 tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga gaattaagaa
     7561 aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt atacatgtca
     7621 acgataatac aaaatataat acaaactata agatgttatc agtatttatt atgcatttag
     7681 aatattaaaa ttccttcatt acactcttgg cggtttcact tatcaactta tcatttggct
     7741 tatcactttt attgtcttta ttcgtaaaaa tgactaaaac aataggttca gattggccct
     7801 taggataaac aaaagcaaca tcatttctag aagcatatgt tattgcttga ccacttttat
     7861 cagcaacctt atagtctttt ggaacaccat ctttaattaa agtgtctccg tttttattat
     7921 taaacattaa atcaagtaag aaatttttat tttttttgct taattttcca tttgcgataa
     7981 gtttatttaa agtcttgccg aaagcagcag gcgttgaagt atcttttttg ctctttggtg
     8041 aatagtaatt taattctatc tcatatctaa ctggatttgt tactttatct cctagttctt
     8101 ttagacgttg tttaactttt ttgattccac cgatttcttt tataatttta ttgtttgctg
     8161 tattatcact atatgtcatt gaagcctcaa taagttcttt taaagtgata tcttttccta
     8221 catatttttc taaaatagga gaataagcaa ctatatcatc tttgttaata tgtacttttt
     8281 tatttaactt attataaggt acttgttcta acaaaatagc actatttatc gcttttgaag
     8341 tcgaagcata ggcaaatctc ttatctgaat taaattttac ttccttacca cttttagtat
     8401 ttaaagcata aacaccaata tgagcattat attttttttc taaatcattt aactctttgg
     8461 catgtgaact gtttgaatta catgcactta aaactaaagc aattacaatt aaaaatatta
     8521 actttttcaa aataaaccct ccgatattac agttgtaatt tttataaatc aataatatta
     8581 caattgtaat attgatgtca attgtttatt ataaatattt gaaagaaggt gtcaaaatgg
     8641 ctaaattact aataatgagc gtagtaagct tttgttttat atttctattg ttagtatttt
     8701 ttaggtacat attaaaacgc tattttaatt atagtttaaa ttataaagtt tggtatctaa
     8761 ctatgcttgc tggattaatt ccttttatcc caattaaatt ctcttttata aaatttaata
     8821 atgtgaataa tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac cataacataa
     8881 ataccaccaa acctattcaa gagttcacaa cagatatcca taagtttaat tgggattcaa
     8941 ttgataatat ctgcacagtt atttggatag ttttagttat tattttaagt tttaaatttt
     9001 tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta aacgaaaatg
     9061 aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaaat attgtgattc
     9121 gaaaagcaga ggctattcaa tctccaataa ctttttggta tgggaaatat attattttga
     9181 ttcctagttc atattttaaa agtgtaattg ataaaagact aaaatatata atactgcatg
     9241 aatatgctca tgctaaaaat agagatactt tacatttaat tatctttaat atcttctcta
     9301 ttattatgag ttacaatcca ttagtacata ttgtaaaaag aaagataatt catgacaatg
     9361 aagtagaagc cgatagattt gtccttaata atattaacaa aaatgaattt aaaacttatg
     9421 cggaatctat tatggactcc gtattaaaaa ccccattttc caacaaaaat atattaagcc
     9481 attcatttaa tggtaaaaaa tcattactca aaagtagatt aattaatata aaagaagccg
     9541 atctgaaaaa gcagtcaaaa ttaattctaa tttttatttg tatttttact ttttttatca
     9601 tgataattca aagccagttt ttaatggggc aatccttaac tgattataat tataaaaagc
     9661 ccttacaaag cgattaccaa attttagatg aaagtaaaaa ttttggttca aatagtggat
     9721 ctttcgttat gtacagcatg aagaaagaca aatactatat ttataatgaa aaagaaagta
     9781 gaaaaagata ttctcctgat tcaacttaca aaatttattt agcactgttt ggacttgacc
     9841 gtcatattat aagtgataaa aattcacgta tgtcatggaa tcataaacat tatcttttcg
     9901 agtcttggaa taaggaacaa gatttaaata cagcaatgca aaattcagtt aattggtact
     9961 tcgaacgtat tagtaatcaa ataccaaaga actatactgc ggctcaactc aagcaattaa
    10021 attatggtaa tgaaaatttg ggaagttata aaagctattg gatggaagat agtttgaaaa
    10081 tatctaatct tgaacaagta atagttttta aaaatatgat ggaacaaaat aaccatttta
    10141 gtaaaaaagc aaagaatcaa ttatcttctt cattattgat taagaaaaat gaaaagtatg
    10201 aactgtatgg gaaaacaggt acaggtatag taaacgggaa gtataataat gggtggtttg
    10261 taggttacgt aattacaaat catgataagt attattttgc tacacattta tcagatggaa
    10321 acccgtctgg gaaaaatgct gaattaataa gtgaaaaaat attaaaagga atgggtgttt
    10381 taaatgacca ataagcaagt tgaaatatct atggctgaat gggatgttat gaatataata
    10441 tgggataaaa aatcagtatc agctaatgaa attgtagttg aaattcaaaa atataaagaa
    10501 gttagtgata aaacgattag aacattaatc acaagactat ataaaaaaga gattataaaa
    10561 cgatacaaat cagaaaatat ttatttttac tcatcaaata ttaaagaaga cgatattaaa
    10621 atgaaaactg ctaaaacctt tcttaataaa ctgtatggag gggatatgaa aagtttagtg
    10681 ctgaattttg cgaaaaatga agaattaaat aacaaagaaa ttgaagaatt gcgagacatt
    10741 ttaaatgata ttagtaaaaa gtaattagag gtatgtttca aacataatac atatgtaaac
    10801 catacttgac tcttattaaa ttagttttgt taatgacatt taaaatatat tattaaagat
    10861 gagagtgtcc agaaaataac cattttctgt acactcttgt tttttgtcta aataagtaag
    10921 tattacatag aattatgtgt tcaaaaaccc aacaaaaaat ctatgttcac aaaagacctc
    10981 gtaatcaagt tatggtacac taaaggtgta tataggaggt ctgtattttg aaaataggtt
    11041 atgcaagagt ttcaactgga ttacaaaatt tgaatttaca ggaggatcga ttaaatacat
    11101 atggttgtga aaagatattt aatgaccata tgagtggttc aaaaagtaag cgacctggtt
    11161 tagataaagc aattgaattt gcgaggtcag gagatacgat tgttgtttgg agattagata
    11221 gactaggacg gaacatggag gatttaatca cgttagttaa tgaacttaac gaacgaggtg
    11281 tgagttttca tagtttagaa gaaaatataa cgatggataa atcaagttct acaggacaat
    11341 tattatttca tttattcgca gcatttgcag agtttgaacg taatttaatt ttagagcgat
    11401 catcagccgg tcgaattgca gcacgtgcta gaggccgata tggaggtaga cctgaaaaat
    11461 taaataaaca agatttaaac ttacttaaga cactttatga taatggtacg ccaattaaaa
    11521 caattgcaga gcagtggcaa gtttcacgta caactattta tcgttatctc aataaattag
    11581 aggcaaaaga gaataaaaac aagtagagca actaatctag cttactataa tgaatctact
    11641 aaataatttt atatatcatt aacatacaaa gactttgatt caatgtcact cgatgtaaaa
    11701 gagacgttct gacagcgtct aaaatctcat aatattttcc tgttatgaga ttttaataat
    11761 aacacaagaa aaaagcgtac aaattcgtac gcttttttaa tataatacct gtttcactaa
    11821 tgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt tgaaatactt
    11881 tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac taaaatttta
    11941 taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca aaaccccttt
    12001 ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt tagattagct
    12061 ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg acttccagat
    12121 atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt aatatttaca
    12181 ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt agtagatgta
    12241 tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt ttgaatcttt
    12301 ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct ttcttgcaac
    12361 tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc aatttgatta
    12421 tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct tttcgattca
    12481 aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc aaaaccatat
    12541 ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc ttctttaata
    12601 aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt attaaaaatc
    12661 gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat atcatcaacc
    12721 ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg cttaataata
    12781 aagtgattaa taaataaaaa taaacatcca tcaacctttt tatcaagggt ttatgaaagg
    12841 tttatgatat ttatcaaggg tttataaacc cttgataaag ctatattttc ccttttataa
    12901 gtttatttaa agtgctataa taaacttaat ataacaaaaa ccccaactac tggtaatagt
    12961 cggggttttt gttatattaa gtttattata gcactttaaa taaacttata aaagggaaaa
    13021 tatagcttta tcaagggttt ataaaccctt gataaatatc ataaaccttt cataaaccct
    13081 tgataaagct atattttccc ttttataagt ttatttaaag tgctataata aacttaatat
    13141 aacaaaaacc ccaactactg gtaatagtcg gggtttggta ataaaacgtt tagaaactgt
    13201 ttatttatat aattttcgat acttgtatta tataatgaat aatatcttag tgcaagcacc
    13261 taaatcatta aataaatgta aagctaaata aaaacgtttt attacctctt accaaactaa
    13321 aatatggaaa gaggtttttt gtcatgcaaa atcaatattt taca
//
