LOCUS       NZ_CP151002            18413 bp    DNA     circular CON 16-APR-2024
DEFINITION  Escherichia coli strain CUVET16-321 plasmid pCUVET16-321.2, complete
            sequence.
ACCESSION   NZ_CP151002
VERSION     NZ_CP151002.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40623351
            Assembly: GCF_038096125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18413)
  AUTHORS   Sroithongkham,P., Leelapsawas,C., Indra,R., Nittayasut,N. and
            Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli,
            complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18413)
  AUTHORS   Sroithongkham,P., Leelapsawas,C., Indra,R., Nittayasut,N. and
            Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2024) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAR-2024
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 67x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096125.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 23:06:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,932
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,572
            CDSs (with protein)               :: 4,572
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 142 of 239
            Pseudo Genes (incomplete)         :: 98 of 239
            Pseudo Genes (internal stop)      :: 28 of 239
            Pseudo Genes (multiple problems)  :: 27 of 239
            Pseudo Genes (short protein)      :: 2 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET16-321"
                     /isolation_source="urine"
                     /host="Dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET16-321.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2016"
     gene            385..468
                     /gene="dqlB"
                     /locus_tag="AACG84_RS24545"
                     /old_locus_tag="AACG84_24545"
     CDS             385..468
                     /gene="dqlB"
                     /locus_tag="AACG84_RS24545"
                     /old_locus_tag="AACG84_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_272056275.1"
                     /translation="MMNLLIIVLRAVLAIANALIAIQKLIE"
                     /name="dqlB"
     gene            1029..1124
                     /gene="dqlB"
                     /locus_tag="AACG84_RS24550"
                     /old_locus_tag="AACG84_24550"
     CDS             1029..1124
                     /gene="dqlB"
                     /locus_tag="AACG84_RS24550"
                     /old_locus_tag="AACG84_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609146.1"
                     /translation="MINLLIIVLRAVAALANALIAVLELIRQFID"
                     /name="dqlB"
     gene            1191..1370
                     /locus_tag="AACG84_RS24555"
                     /old_locus_tag="AACG84_24555"
     CDS             1191..1370
                     /locus_tag="AACG84_RS24555"
                     /old_locus_tag="AACG84_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000347021.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRFTGMFMSFIVGCLS
                     WYITKPKPQKAEVA"
                     /name="hypothetical protein"
     gene            complement(1486..2130)
                     /locus_tag="AACG84_RS24560"
                     /old_locus_tag="AACG84_24560"
     CDS             complement(1486..2130)
                     /locus_tag="AACG84_RS24560"
                     /old_locus_tag="AACG84_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203199.1"
                     /translation="MTSNAPTNFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSAIKRKSLQSSGE"
                     /name="recombinase family protein"
     gene            2425..3045
                     /locus_tag="AACG84_RS24565"
                     /old_locus_tag="AACG84_24565"
     CDS             2425..3045
                     /locus_tag="AACG84_RS24565"
                     /old_locus_tag="AACG84_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864791.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000864791.1"
                     /translation="MKVISFLNPKGGSGKTTAVINIATALSRSGYNIAVVDTDPQMSLT
                     NWSKAGKAAFDVFTAASEKDVYGIRKDLADYDFAIVDGAGSLSVITSAAVMVSDLVIIP
                     VTPSPLDFSAAGSVVTVLEAQAYSRKVEARFLITRKIEMATMLNVLKESIKDTGVKSFR
                     TAITQRQVYVKSILDGDSVFESSDGAAKGEIEILTKEIVSTFE"
                     /name="ParA family protein"
     gene            3097..3327
                     /locus_tag="AACG84_RS24570"
                     /old_locus_tag="AACG84_24570"
     CDS             3097..3327
                     /locus_tag="AACG84_RS24570"
                     /old_locus_tag="AACG84_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="WP_000051063.1"
                     /translation="MSLEKAHTAVKKMTFGENRDLERVVTAPVSSGKIKRVNVNFDEEK
                     HTRFKAACAKKGTSITDVVNQLVDEWLKENE"
                     /name="plasmid partition protein ParG"
     gene            3343..3654
                     /locus_tag="AACG84_RS24575"
                     /old_locus_tag="AACG84_24575"
     CDS             3343..3654
                     /locus_tag="AACG84_RS24575"
                     /old_locus_tag="AACG84_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001423339.1"
                     /translation="MDKNVVDALKTLLEALREEVVTEVTSKLNPSASHIPEENSKQMTA
                     KARLLNFRLTEAYEEILEAEAIRTGQSKTTVLKAALAAFDSLDENRKNHWLLESAKLG"
                     /name="hypothetical protein"
     gene            3841..4065
                     /locus_tag="AACG84_RS24580"
                     /old_locus_tag="AACG84_24580"
     CDS             3841..4065
                     /locus_tag="AACG84_RS24580"
                     /old_locus_tag="AACG84_24580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341516201.1"
                     /translation="MRIFIVLAGLLLGCWNLFDNYKSYKKGVYKEHRKMAPPVYYYRGD
                     HTFVIRIVIDSLLSLVIIGFVVWFWFKTA"
                     /name="hypothetical protein"
     gene            4089..4685
                     /locus_tag="AACG84_RS24585"
                     /old_locus_tag="AACG84_24585"
     CDS             4089..4685
                     /locus_tag="AACG84_RS24585"
                     /old_locus_tag="AACG84_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="WP_040121203.1"
                     /translation="MASDNRYSINFTLSVLEKTEASKLIKEHEKQEHIEPYGYILLIKK
                     AALLVLSFAALFDFIILFGLIALAPKDPGYVERAKFLSIITFTLIAIFIGLLYIYDYLM
                     KKVMRTNYLSEIRDSELMLRLKINRSYIEQIMKIGSSKVFWNNIKKTYREKGFLFIQTK
                     ENRCIIIPERVFKNGEETEKLYNFVKEQIAKNTIE"
                     /name="YcxB family protein"
     gene            complement(4737..5093)
                     /gene="ddp1"
                     /locus_tag="AACG84_RS24590"
                     /old_locus_tag="AACG84_24590"
     CDS             complement(4737..5093)
                     /gene="ddp1"
                     /locus_tag="AACG84_RS24590"
                     /old_locus_tag="AACG84_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_341516202.1"
                     /translation="MKYLVKEFINEKYTKVVNILKDNLKEHYHVFYCVRLSEILFPASE
                     YGTEQFFSDFEKINSISLPVLVFDLKEGVPVIVISLDDVSYCDVLDEMDIDFMKCDSLA
                     ELLTSDKLDALFKN"
                     /name="ddp1"
     gene            complement(5233..5676)
                     /locus_tag="AACG84_RS24595"
                     /old_locus_tag="AACG84_24595"
     CDS             complement(5233..5676)
                     /locus_tag="AACG84_RS24595"
                     /old_locus_tag="AACG84_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001074380.1"
                     /translation="MAKIYQFPQGDERGKFRKEIARERKKRFAVKTGSIFVKWLGWGWF
                     YLRLLAASVLHFVSVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWILGLLAEPLMELLNKTMPWHKLLVPEPADESTENSGEISQ"
                     /name="hypothetical protein"
     gene            complement(5680..6522)
                     /locus_tag="AACG84_RS24600"
                     /old_locus_tag="AACG84_24600"
     CDS             complement(5680..6522)
                     /locus_tag="AACG84_RS24600"
                     /old_locus_tag="AACG84_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435061.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_341516203.1"
                     /translation="MEQIKLHKQGGAAVIIIPASMIENTGWEDGTLLDVLEINEALHIR
                     RKTESGALATKACPSLFSSTMRHVNYDKIIRYNSGLNLLVNELSCSARRVFFICLYYYF
                     ENKLESDKSLLIRADKYAEFVGVDCSVAYKQMKDAADFFSRNKKLITHCDYISNEGLLR
                     VMFSSKTINYITAIDSRKYQVTELPLKSALSLQGRYSWPLYQLIKFSHLKSFTIGVDEL
                     KKEIGYIQNQKFSFFKRDGINIAIDEITERTDVKNIKCVNAERQGRRVSKVRFEIEMR"
                     /name="replication initiation protein"
     gene            complement(6543..7382)
                     /locus_tag="AACG84_RS24605"
                     /old_locus_tag="AACG84_24605"
     CDS             complement(6543..7382)
                     /locus_tag="AACG84_RS24605"
                     /old_locus_tag="AACG84_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722379.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_021526660.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDTRIPYGFRGA"
                     /name="replication initiation protein"
     gene            complement(8392..8523)
                     /locus_tag="AACG84_RS24610"
                     /old_locus_tag="AACG84_24610"
     CDS             complement(8392..8523)
                     /locus_tag="AACG84_RS24610"
                     /old_locus_tag="AACG84_24610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001378418.1"
                     /translation="MALSDIALKRSVTRSERFSRFVVVGYGLTVFNAAQRFLAALLL"
                     /name="hypothetical protein"
     gene            8615..8866
                     /locus_tag="AACG84_RS24615"
                     /old_locus_tag="AACG84_24615"
     CDS             8615..8866
                     /locus_tag="AACG84_RS24615"
                     /old_locus_tag="AACG84_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            8856..9137
                     /locus_tag="AACG84_RS24620"
                     /old_locus_tag="AACG84_24620"
     CDS             8856..9137
                     /locus_tag="AACG84_RS24620"
                     /old_locus_tag="AACG84_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9193..9897)
                     /locus_tag="AACG84_RS24625"
                     /old_locus_tag="AACG84_24625"
     CDS             complement(9193..9897)
                     /locus_tag="AACG84_RS24625"
                     /old_locus_tag="AACG84_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            10132..10992
                     /locus_tag="AACG84_RS24630"
                     /old_locus_tag="AACG84_24630"
     CDS             10132..10992
                     /locus_tag="AACG84_RS24630"
                     /old_locus_tag="AACG84_24630"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            <11142..13403
                     /locus_tag="AACG84_RS24635"
                     /old_locus_tag="AACG84_24635"
                     /pseudo=""
     CDS             <11142..13403
                     /locus_tag="AACG84_RS24635"
                     /old_locus_tag="AACG84_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241615.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            13458..13721
                     /locus_tag="AACG84_RS24640"
                     /old_locus_tag="AACG84_24640"
     CDS             13458..13721
                     /locus_tag="AACG84_RS24640"
                     /old_locus_tag="AACG84_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072657.1"
                     /translation="MSNTSYKQIIPATDWYFRHDNVSGVAGKSTVYQLAAWALKENGEV
                     VGLVTVRDDNGRPKLVTPPPVPGDYLHKEQLTDDEKEWAKRR"
                     /name="hypothetical protein"
     gene            complement(13891..15216)
                     /locus_tag="AACG84_RS24645"
                     /old_locus_tag="AACG84_24645"
     CDS             complement(13891..15216)
                     /locus_tag="AACG84_RS24645"
                     /old_locus_tag="AACG84_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_000201787.1"
                     /translation="MATKKYELTKEYFFHGEFWHQLDDNKGRFSARIEYSPYHGLILDY
                     CISDSESPRTCEILYGVLNTGERCTLIGKFDFTQGNIHFDKGIIHTGRHGFPIMLFNDF
                     YAPDSKIEYCDLSLHGLQEFIHPHGFFTQLKHLEHPIFIAKGNHWTLQLVNHVSFSVIG
                     DDLLNIINCQNKAALENIIHQLKKTKELYPDAFFSIRKELVFYFRIKSSNDLGIEDHIS
                     KCWDISGLFSILLNKPTLPEEINIKFKGNGSKTPCLLTTGFEQRTIDLALREIKHQLLP
                     INRKHINLGKIFCKWFKIAERYMPLTITYQYETGFRTLHQAHTDIILFATQLEAINKTI
                     GGSKNEKYMKPINEYASLFLIQEIEMFFKKFNNKSIGENIATLRNELAHVDRKKELMNI
                     LTIGDYVKIGNYLKTIVTSYLLSDLGINNIIIEKYQAQTIQE"
                     /name="HEPN domain-containing protein"
     gene            complement(15267..16058)
                     /locus_tag="AACG84_RS24650"
                     /old_locus_tag="AACG84_24650"
     CDS             complement(15267..16058)
                     /locus_tag="AACG84_RS24650"
                     /old_locus_tag="AACG84_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_341516200.1"
                     /translation="MGTTKDWVIQVEESRREEWIRERLSSPNLEEDSEEWQLLEKDYDE
                     YQDFLSDMAMEEYETEKWLKQHPHTEIYKIAINLLEQIKEEGKQSTSEVFIKMKIAYIV
                     TIMENCLSEMIKSVVLSHNRYVENAIRNINELKAKNISLSELINKESNANKYVQEYLSD
                     ILYHRIQLVVEIYKAVLQPKQYPRFPLKNINELMKLRHDIVHRNGKTKTTDEKIHTFNT
                     ATLNDAFKVVEEFLNNMMNLISDAVEHHENEQIARDLEDEF"
                     /name="HEPN domain-containing protein"
     gene            complement(16265..16840)
                     /locus_tag="AACG84_RS24655"
                     /old_locus_tag="AACG84_24655"
     CDS             complement(16265..16840)
                     /locus_tag="AACG84_RS24655"
                     /old_locus_tag="AACG84_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="WP_001505426.1"
                     /translation="MTQSEMLKLIARHGYNVGFGAKKNFATYDLICKASGWISFISLAV
                     GVFALFIPELATNVISAILIIFGVATMYIQFYDSEKQSYESAGIEQTKIFNELEVIYRN
                     VKSDANFDYQHTQQSIDAIMSRFYGTVISKQIFGSNWYAHYKFYCEFEKTWVEEELNLT
                     LKDKIPLSLCFLAGLAIIFIIAVIWKML"
                     /name="SLATT domain-containing protein"
     gene            complement(16848..18177)
                     /locus_tag="AACG84_RS24660"
                     /old_locus_tag="AACG84_24660"
                     /pseudo=""
     CDS             complement(16848..18177)
                     /locus_tag="AACG84_RS24660"
                     /old_locus_tag="AACG84_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /name="nucleotidyltransferase"
     ARG             10133..10989
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5234..5676)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.973"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttgtgtt ttccagcctt gagttggtgg ctctgactgg aggaagatac tctacaccca
       61 acactcatag tattatggca ctagacacct gttgtctagt ttgacttcag attgtctgtc
      121 aatggtaatt tctttaaatg aattttatgc atgcatctgt ataatctttg tgttggcgct
      181 gtacagtgtg tctcccttgc ttctaacccc tgcgctatct ggggatgaac gaattaaagt
      241 cctggctgaa caaacgggtg gtgctgtgtt cagtgctctg gtagagggaa ggtactctac
      301 atccaacaat gtgagagagc cgttgtgaca gggatgtatg agcaagctac ggtgacttcc
      361 tccagtatca acctagagaa acggatgatg aatctgttaa tcatcgttct cagggctgtg
      421 ctcgccattg caaacgcgct gattgccatt cagaaactaa tcgagtgatt gatggcttaa
      481 ctaagggaga gacgtttacc gcttcctgcc ctttaacaaa ggagtgttta tgagttttga
      541 acaattattt acactaacga cccaacgtcg ttgggtttgc cgcagaaaat ttattcattt
      601 aaaatagatc taaatgaagc gggaaactgc agacgtaaaa aaaccgactg gtgaggtcgg
      661 cttttttaca gttccggtac gagctggtaa ctcgcccgga agagaaactc tctacaactg
      721 acaacagtat gataatccgt gaagcctgat tatgcaatat gacaggcggt gactacgcgt
      781 taacggtttg cttgaaagtt agataaacgt ataatcagag ggttagtgct gtggtgagtc
      841 tttccctgca ttccgctaaa tgcggcatga acagcttcaa agtcctgaca ggtaatacag
      901 gtggtgctgg ttatctgtgc tctggtagga gtgagactct ctacaactga caagacgaag
      961 agaagccgtg gtgacaggca gcgtaagagc aagccacggt gtctttactc catttatgga
     1021 gaaaacggat gattaacctg ttaatcatcg ttctgagggc ggtagctgcg cttgcaaacg
     1081 cgctgattgc agttctggaa ctgatccgtc agttcatcga ttgatgactg cggaaacgta
     1141 actaagggca ggaagtgcac cgcttcctgc cctttaacaa aggaggtgtt atggatttta
     1201 tgagcatttt ggggcgctgt ttacttcttc ctttccctgt tgcgatgttt gtcggttgcc
     1261 tgtttccagg catcgatgat cgttttactg gaatgttcat gagttttatt gtcggttgtc
     1321 tatcgtggta tatcaccaag ccaaaaccgc aaaaggctga ggtcgcgtaa gcggcctttt
     1381 attttgatac tcattagtta caacattcac tttgcttttt aaaaatatcc acttcagcac
     1441 ctcactcata aataacgtca accagtttat tgagctcacc ctaccctact caccggagga
     1501 ttgcagggat ttacgtttta ttgcgctact gattgtgccg cggctgcacc ctagcacctt
     1561 ctgtacctgt gtccaggagc tgccactttc tatcaacctg ataattgcat catgtctggc
     1621 ttgattaggt ttacgccctt tatatttgcc gtctttcctt gctttctcta tcccctgttt
     1681 ctggcgttcc cgccgttgtt catagtccct tctggcaaca gcggccagca tatccagcag
     1741 catatcgtta atggctgcaa acaaccggct gtcaaactca ctcataccag aattaatcca
     1801 ggtcgtcggc acattcacgg ccataacccg gatatctttc tgacggatca ttttcttcag
     1861 cgtattccag tcttcccctg acagtcggga aagtctgtcc acatcctcga taagcaaaat
     1921 gtcattttgc tggcaatctc tcagaagacg gaagagttcc gggcggtcaa gacgggagcc
     1981 ggattcattc tcaatataat agctgcaaat gatcaggcct ctttccctgg caaaagcctc
     2041 gatagtttcc agggcacgcg aagcatcctg ttctgctgtt gaagctctca gataagcgcg
     2101 gacaaagttt gttggtgcat ttgaagtcat attacagaac cagttcgcat aaggtcacac
     2161 cactataccc aaaatgaacc agaaagtgga tcgacaggaa aggcacactc taaaagaacc
     2221 aaaacacaaa taatgagtag atgtgacctt gtcaccatat actcataaac tcaaatactc
     2281 acatacacaa tacaactcaa atgagtaaac actctttaac tcatatacac atagactcat
     2341 attaaccttt actcatacaa agagtatggt aaactcaaaa actcacagga gtaattgagt
     2401 agatacacaa ttgagagttt gagtatgaaa gtaatctcat ttctgaatcc taaagggggt
     2461 tcaggcaaaa caactgccgt aatcaacata gccactgcgt tgagcaggag cggatacaac
     2521 attgctgtgg tagacacaga tccacaaatg agcctgacga actggagcaa agcaggcaag
     2581 gcagcatttg acgtatttac agcagcatct gaaaaagacg tctatggaat ccgaaaagat
     2641 ctggcggact atgattttgc tattgtggac ggagcaggtt cgctctcagt aatcacctcc
     2701 gcagccgtca tggtaagcga tctggtaata atccctgtca cccccagccc cctggatttc
     2761 tccgcagcag gaagcgtcgt tactgttctg gaagcacagg cttacagccg caaagttgaa
     2821 gcccgctttc tgatcacccg taagatagaa atggcaacca tgctcaatgt gctgaaagaa
     2881 agtatcaaag ataccggtgt taaatccttc cgtacggcca ttacacaacg tcaggtttac
     2941 gtgaaatcaa ttctggatgg tgacagcgtg tttgaatcca gtgatggtgc agcaaaaggt
     3001 gaaatagaaa tccttacaaa agagatagtt agcacatttg agtaattaca catttactca
     3061 tataatcaaa taagtataac aaccggagaa agcttaatgt cacttgaaaa agcgcatacg
     3121 gcagtaaaaa aaatgacctt tggtgaaaac agagatctgg aacgagtagt aacagcacca
     3181 gtatcatcag gaaaaatcaa acgtgttaac gtcaattttg acgaagaaaa acacacccgg
     3241 tttaaggctg catgtgccaa aaaaggtaca tcgatcactg atgtagtgaa ccagcttgta
     3301 gatgaatggc tcaaagagaa cgaataatat ctgaggaata tcatggataa aaacgtcgtt
     3361 gatgcgctga aaacgctatt agaagcgtta cgggaagaag tggtaacaga agtcacatca
     3421 aaactaaatc cttcggcaag ccatattcct gaagaaaaca gtaaacaaat gacagcaaaa
     3481 gcaagactac tgaatttccg gctaaccgaa gcctatgaag aaatcctgga agctgaagct
     3541 atcaggactg gtcagagcaa aacaaccgtt ctcaaagcgg cgctggccgc tttcgatagc
     3601 ctggacgaga accggaaaaa ccactggctg cttgaatctg caaagttagg ttagcattat
     3661 ggctaagaat gatagatatg ttgttatggt tgggaataaa accatataca gcggtaatca
     3721 gcgattttta gcctggttag tatggttagc tcacaggtac aataaagcca ttgcctgtga
     3781 caacgggata tggattgtcg agccatctta ctggttgagg actggaaagg aaaaatgata
     3841 atgagaatat tcattgtatt agctggtctt ctcttgggat gctggaactt atttgataac
     3901 tacaaatcct ataaaaaagg agtttataaa gagcatcgca agatggctcc tcctgtttat
     3961 tattatcggg gagatcatac ttttgtcata cggatagtaa ttgattcact tttaagtctg
     4021 gtgattattg gctttgttgt ctggttctgg tttaaaactg cttaacatca taacatcgag
     4081 gaagcaaaat ggcatcagat aatagataca gtattaactt cacgctatct gttttagaaa
     4141 aaacagaagc aagcaagctg ataaaagaac atgaaaaaca agagcatata gaaccatatg
     4201 gctatatatt gcttataaaa aaagcagcgt tgttagttct ttcatttgct gcattatttg
     4261 attttataat actttttggt ttgattgctc tcgccccaaa agaccctggt tatgtagaaa
     4321 gagcaaaatt tctttccatt attacattta cactaatagc tatatttatc gggttattat
     4381 atatatatga ttatttgatg aaaaaagtaa tgaggactaa ctatttatca gaaataagag
     4441 attctgaact aatgttacgt ctcaaaataa atcgttcata cattgagcaa atcatgaaaa
     4501 ttggtagttc gaaggttttc tggaataaca taaaaaaaac ataccgtgag aaaggatttc
     4561 tatttatcca gacaaaagaa aacagatgca taatcattcc agaaagagtt tttaaaaatg
     4621 gagaagaaac cgaaaaatta tataattttg taaaggagca aatagcaaaa aacacaatag
     4681 aatgaaaaag gaaaaacaaa aaggaagttt aagtcgcaga gaaggggaca taagactcaa
     4741 ttcttaaata acgcatccag cttatcgctg gtaagcaatt ctgccagcga atcacatttc
     4801 ataaaatcaa tatccatttc gtccagaaca tcacagtagc tgacatcatc aagactaatg
     4861 acaatcacag gaacaccctc tttgagatca aacactaaga caggtaatga aatggagttt
     4921 atcttttcaa aatcactaaa aaactgttca gttccatatt cactggcagg aaaaagaatc
     4981 tcacttaatc tcacacaata aaaaacatga tagtgttctt taaggttatc ctttaaaata
     5041 ttaacaacct tagtatattt ttcgttaata aactctttga ccaaatattt cataaaactc
     5101 tccaacaaga accgactgta ggtcatcggg caaacgttgc ggaatggcgt cagagacgtc
     5161 attttacggc gtttgcccta tcctgcatcg cagtgaaaaa gcaggaactg gcataacggc
     5221 tgagtagcat cattactggc ttatctcgcc actgttctcg gtgctttcat ccgcaggttc
     5281 gggaacaagt aacttgtgcc agggcatagt tttgttgagc agctccatca gaggctcagc
     5341 cagcagtccc aaaatccagg ttgccgtaat caccggtatg gtcagatttt gtggcgtcca
     5401 taactggtga tcgagatgat accaggtgat gacacacaaa atccccccca gccagaacac
     5461 agcccattta aacgcaccaa gaatggctaa cacaaaaaca gagacaaaat gtaaaacact
     5521 ggcggccaga agacgaagat aaaaccatcc ccagccaagc cacttaacaa aaatgctccc
     5581 tgttttcacc gcaaaacgtt tcttacgttc tctggcgatt tctttcctga atttaccgcg
     5641 ttcgtccccc tgggggaatt gatagatttt agccatgagt tacctcattt caatctcaaa
     5701 acggactttg ctgactcttc taccctgcct ttcagcatta acacatttaa tgtttttaac
     5761 atctgtcctt tctgttattt catcaatagc tatattgatt ccatctcttt taaagaaaga
     5821 aaacttttga ttttgaatat aacctatttc tttcttaagt tcatctacac ctatagtgaa
     5881 agatttaaga tggctaaatt ttatcagttg atataaaggc catgagtatc ttccttgaag
     5941 agacaaagca gattttagag gaagctcggt aacctgatat ttacgggagt ctatcgctgt
     6001 aatataattt atcgtcttag acgaaaacat aacacgaagc aatccctcat tcgaaatata
     6061 gtcacaatgt gtaattaatt ttttatttct tgaaaagaaa tctgctgcat ccttcatttg
     6121 cttatatgcg acagaacaat caacaccaac aaattctgca tacttatcag ccctgatcaa
     6181 tagcgactta tccgattcaa gtttattttc aaagtaataa tataaacaaa taaaaaagac
     6241 ccttcttgcc gaacatgaca gttcattaac taacagattt aatccgctat tatatctaat
     6301 gattttatcg taattaacat gcctcatagt agatgaaaaa agagaaggac aagctttagt
     6361 agctaaagct cctgattctg tctttcttct tatatgaaga gcctcattta tttctagtac
     6421 atccaataag gttccatctt cccatcctgt attttctatc atactggcag gaatgataat
     6481 tactgccgca ccaccttgtt tatgtaattt tatttgctcc atagtgtatt tacctttata
     6541 tattaagcac ctctgaaacc atacggtatt ctggtatcaa gtccttcgct ttctttatct
     6601 attttatacg aaaaccttag ctttgatgct cttcttgcga ctctctcaac gattttaact
     6661 tcgagatgat taatctctgt atattgattt attttttcaa tatttttttg cagaaaaacc
     6721 ttattaaaat ccttaaaatt ctgataaagg taaacttttt cgccattatc tattctgaat
     6781 aattcaagtt catcctttaa tacatctact tcaatgtcga aaaatgtctt tttaccactg
     6841 ctgtattgtt tcctgataaa ctgataaagc atatttgaat tttgatcagc gagtcttaca
     6901 gacgatatca aaacctgagt agtgtaagaa tcctttaaca tcgtgatata tggctcaatg
     6961 acatgtgaaa aagcaatatc aatatatcct tcactttcaa cataatcaca aaacgcagtc
     7021 aggttcataa gcctgactgc atcagaaggt aatttacctc tcttcttcgt atgtccagag
     7081 ctttgattgc cctgaggtgt gagaagttga tttcgaggaa tgcgaataac actggcctga
     7141 agttcttctg ccccctcttt caactgccgg taagcacctg ttacatcaat atcagcgata
     7201 aatgcatatt cccgagccgt aatacgaaag gttgccccct cagataactc ttcacgtgaa
     7261 tcaatctgcg ccatagccat aaacaaaatt cttctggcag acaaaggcaa cgatgagaat
     7321 gtgctgttaa tttcatttct gtggcgaatt tttgttttct ttgtaacgct cagtaagttc
     7381 atacattacc tgtggttttt aagttctgat ggtgaaatca taaccacaaa ggcggaaata
     7441 tgtaaagtgg tttatccacc ttatgtattt ccaccaaaac acggatcaca tgaaagaatt
     7501 tttatcattt atattcaata tgatagaaat gcgcaggtat ccacctttta acgtcatacg
     7561 ttaaattaga gcactataaa gaactaaaga aatgaagata caaagatacg aatatctgaa
     7621 taaaaacaga aaaatgcgta cccatccacc tttcagtgcg tacccatcca cctttcagtg
     7681 cgtacccatc cacctttcat gaaaattaca gtgcgtacct atccaccttt cagtgcgtac
     7741 ccatccacct ttcattttga ggcgacaaaa aacaacacct atcttaatga aattattgat
     7801 aaaaaagctg gcacaatgtt tgcagaataa gttaaagtaa gttaaagatc ttaaagtaga
     7861 tctaccgatc tacatttaag atcttaaaaa caagaagttt aataagcaaa aaaacacaaa
     7921 aaatctgtgg ataactctgc tcaaaacaaa aaattcacag cgcctgtgga taactttgcg
     7981 taaacccgga ggacatatca ctctgaacca agacaaccac acataaaaac aatatgctca
     8041 ttttttaacc actgtgcgta agtgcctaat gtaatccctg tgctattccg cttcctcgct
     8101 cactaactcg ctacgctcgg tcgttcggct gcggcgagcg gtgtctcctt tctcaaaacc
     8161 agaagacttc agatcatacg atccgttgat tgatcctttt tggatctttc actgagaagc
     8221 cctgaatcgc tttctgccgc ttcgttcgat acaaccatgg tgaaaccatg cgaaatcgaa
     8281 aaaatcgctc agaatgcgat acagcgcgtt ttagagggta tttacaaaac agtcgttgcc
     8341 ttgaggaaat tttgatcaag attgaactcc tatccggatc atggcaacag atcacagcaa
     8401 caaggccgct aaaaagcgtt gagcggcgtt aaacaccgtc aaaccgtagc cgactacaac
     8461 gaaacgggaa aaacgctcag aacgcgttac agagcgtttt aaggcgatgt cggaaagagc
     8521 catatctcac ctattctgat gtttttctca aaatgagcac ttttaagagt gcttttaaag
     8581 atgctattat gccaatgaga caagcggagg gcttatgagc tatcaaattc tgacaaccac
     8641 agcggccagt attactgacc tgaaaaaaaa tcctatggga accgtagctg aaggtgaagg
     8701 ggacgctgtt gcgatcctga accgaaacga acccgcgttc tattgcgttc caccaaaact
     8761 ttacgcctac tatcgggaac tcgctgaaga tgctgagtta aacgctgttg ctgatgagcg
     8821 catgaaaaac ccggaaattg tgaaggttaa cctggatgac ctatgaactg gcttttgacc
     8881 gcagagcact gaaggaatgg cagaaactcg gccacaccat ccgtgaacaa ttcaaaaaga
     8941 aactggcaga acggctggaa aatccacgcg tacccgcagc ccggttacat ggtcatgctg
     9001 atcgctataa aatcaaactt cgtgcatctg gctacagact tgtatatcaa gtcattgatg
     9061 agaaagtcgt tttactcgtt attgccgttg gaagaaggga aagcagcgaa gtctatcaga
     9121 tcgcagattt gcgctaagat ggcactgttg caaagttagc gatgaggcag ccttttgtct
     9181 tattcaaagg ccttacattt caaaaactct gcttaccagg cgcatttcgc ccaggggatc
     9241 accataataa aatgctgagg cctggccttt gcgtagtgca cgcatcacct caataccttt
     9301 gatggtggcg taagccgtct tcatggattt aaatcccagc gtggcgttga ttatccgttt
     9361 cagtttgcca tgatcgcatt caatcacgtt gttccggtac ttaatctgtc ggtgttcaac
     9421 gtcagacggg caccggcctt cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc
     9481 tttatccgtg ttgatgaatc gtgggatctg ccacttcttc acgttgttga ggattttacc
     9541 cagaaaccgg tatgcagctt tgctgttacg acgggaggag agataaaaat cgacagtgcg
     9601 gccccggctg tcgacggccc ggtacagata cgcccagcgg ccattgacct tcacgtaggt
     9661 ttcatccatg tgccacgggc aaagatcgga agggttacgc cagtaccagc gcagccgttt
     9721 ttccatttca ggcgcataac gctgaaccca gcggtaaatc gtggagtgat cgacattcac
     9781 tccgcgttca gccagcatct cctgcagctc acggtaactg atgccgtatt tgcagtacca
     9841 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     9901 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     9961 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
    10021 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    10081 atgagacaat aaccctggta aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    10141 caacattttc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    10201 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    10261 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    10321 tttccaatga tgagcacttt taaagttctg ctatgtggtg cggtattatc ccgtgttgac
    10381 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10441 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    10501 gccataacca tgagtgataa cactgctgcc aacttacttc tgacaacgat cggaggaccg
    10561 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10621 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
    10681 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    10741 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10801 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10861 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10921 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10981 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    11041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    11101 ccttaacgtg agttttcgtt ccactgagcg tcagacccct agatcgtacc attcaccaga
    11161 accggttgct gaaactggcg cgggaaggcc gaaagatgag tagccgggat ttgacccgat
    11221 tttcagctgc ccggcgtcat gcgatcctgg tgtgcgtgct ggaagaagcc agagccacac
    11281 tgacagacga agtgattgag ctgcatgagc gaatgctgaa tagtcttttc agcaaagcca
    11341 aacgaacaca ggctgagcgc cttcagcaga ccggaaagct gatccagtca aaactgaggc
    11401 agtacatcga tgtcggtcag gcgctgtctg atgcccgtga ttccggtggc gatccctggc
    11461 tcgcaataga aaacatactc ccatgggctg aatttgtcgc cagtctggat gaaacacgcc
    11521 atctcgccag aaaaaataat tttgacccgc tgcatattat taccgagaaa tacagcacat
    11581 tacggaaata tgccccacgc atgttgtccg ctttacagtt agtcgcaacc ccggcagcgc
    11641 aacctctggc tgatgcactg gttgtgatca aggatatgta ccggaagcag tcacgtaaag
    11701 ttccggcggc agcgccgctt gagtttgtcc ctgaaagctg gcgcaaggtg gtgattacac
    11761 ctgtgggtat tgaccggcag tactatgagt tttgtgcact cagcgagctt aagggggcgt
    11821 tgcgctccgg ggatatctgg gttaaaggct cgcgccgcta caaaaatttt gatgattacc
    11881 tcatacccga gaaggacttt gacaaactca caccggcact gccactgccc gtatctgctg
    11941 attatcatga gtacattacc agccgtatga ccctgcttca gtccagactt gaagaggtca
    12001 atgccatggc tgcccttggt gaactgcccg atgtggaaat atccgacagg ggcgtaaaag
    12061 tctctccgct ggataactgc gttccggcgc aggtttcacc gctggcagag ctggtttaca
    12121 gcatgctacc gcgacctaaa atcacggaaa ttctcgatga ggtaaacagc tggacgacgt
    12181 ttacccgaca tttttcgcat ataaaaaatg acattacccg tcccgatacc cgcttgctcc
    12241 tcaccaccat tcttgcggat ggcatcaatc ttggcctgac caaaatggca gaagcctgcc
    12301 ccgggagcac caaatcatca ctcgaagata ttcaggcatg gtacatccgt gacgaaacct
    12361 attcagccgc tcttgcagag ctggtaaatg cgcaggggaa aagaccgttg gcggcatttt
    12421 ggggagacgg aacaacgtca tcatcagatg gacaaaattt cagaaccggc agctcaggcc
    12481 gctacgcagg gcaggttaac ccgaaatatg ggcaagagcc tggacgtcag ttttataccc
    12541 atatttcgga tcaatacagc ccgttttaca cctgcataat cagtcgggtc agggattcaa
    12601 cccatgttct cgatggcttg ctgtaccacg aaagcgacct ggaaatcagg gaacattaca
    12661 ccgataccgc tggtttcacc gatcatgtct ttgccctgat gcatctgttg ggatttgcgt
    12721 tctgtccgcg aatcagggat ctccacgaca aaaaactgtt tatcaaaggg aaagcagaga
    12781 aatacccggc gcttcagtca ctgatatcaa cgaccagcct gaatctgaaa gagatcgaaa
    12841 tccactggcg tgaagtgctg cgtctggcaa cctcgataaa gcagggaacc gtgacggcat
    12901 ccctgatgct gaaaaagctt gccagctatc ccaagcaaaa cggacttgcc aaagcactga
    12961 gagaaattgg ccgcattgag cgaaccctgt ttatgctcga ctggttccgc gacccggctc
    13021 tgcgtcggcg ggtacaggcg gggctgaata aaggcgaagc ccgtaacgcg ctggcgcgtg
    13081 cggtatttat gcaccgactg ggagaaatca gggacagaaa accggaaaat cagagctatc
    13141 gcgccagtgg actgacgctg ctgacggctg ccatatccct gtggaatacc gtctatatgg
    13201 aaagagcggt cgatgccctg aagcgtaaag gggtgaagat caacgagcaa ctgctgtcgc
    13261 atttgtcgcc gttgggctgg gaacacatca atctgacagg tgattatatc tggaaaagca
    13321 accggatacc ggcttctggt aagtttcgtc ggctaagacc agctaaagtc gaaaggtcaa
    13381 aaaacagcct taacgtacaa taattttcga tatccaaact gacccctata ttatcatttt
    13441 gttaaggaag aaaatccatg agtaatacat cctacaaaca aattatccct gcgacagact
    13501 ggtatttccg tcacgataat gtctccggtg tggcaggaaa gtcaacagta taccaactgg
    13561 ctgcatgggc gcttaaagaa aatggtgagg tagttggtct ggtgacggtt cgtgatgata
    13621 atgggcgccc taaactggtt actcctcccc ctgtccctgg tgattatttg cataaagaac
    13681 aactcaccga tgatgaaaaa gagtgggcga agagacgcta aactatattc atataaagcc
    13741 tctgttctag aggctttatt agcatgttta gttgcactgg agttgataat ggcctcaatg
    13801 cgcaaattga ggtaaattga tattgatttt acttttccgt tgctcgtttc tcgtcttatt
    13861 atatttaaat gataattgta taatatttta ttactcttga attgtctgag cctgatattt
    13921 ttcaataata atgttattga ttcctaaatc tgatagcaga taagatgtta caattgtttt
    13981 taggtagttt ccaattttaa cataatcacc aatcgtcaat atgttcatta actccttttt
    14041 gcggtcaaca tgtgctaatt catttctgag tgtggcaata ttttcaccaa tagatttatt
    14101 gttgaatttc ttaaaaaaca tctctatctc ttgaataaga aacaaacttg catattcatt
    14161 aatgggtttc atgtattttt cattttttga gccacctatt gttttgttta ttgcttctaa
    14221 ttgagtcgca aacaatataa tatcagtatg agcttgatgc agtgttcgaa aaccggtttc
    14281 atactgatat gttattgtca atggcatata tctttctgct attttgaacc atttgcagaa
    14341 tatcttccca agattaatat gcttcctgtt tattggaagg agttgatgtt ttatttctct
    14401 cagcgctagg tcaatagttc tttgttcaaa ccccgttgtt aagagacatg gtgtcttcga
    14461 accatttccc ttgaacttta tgtttatttc ttcaggaaga gttggtttgt tcagaagaat
    14521 tgaaaataac ccggaaatat cccagcactt tgatatatgg tcttctattc ctaaatcatt
    14581 agatgatttt atcctaaagt agaaaacaag ttcttttcta atggagaaaa aagcatcagg
    14641 gtataattct tttgtctttt ttagttgatg aattatgttc tccagtgcgg ctttgttttg
    14701 gcaatttatt atgtttaata aatcatcacc aattacgcta aaagatacat ggttgacaag
    14761 ttgaagagtc caatggtttc cttttgcgat aaatattgga tgttctaaat gtttcaattg
    14821 agtgaaaaaa ccgtgagggt ggataaattc ttgcaatccg tgcaaggata aatcacaata
    14881 ttctatcttt gagtcgggag cataaaaatc attgaatagc attatgggaa atccatgcct
    14941 tccagtatgg ataatccctt tatcaaaatg aatattccct tgtgtgaaat caaatttccc
    15001 aattagagta catctttctc cagtatttaa gactccatat aatatttcac atgttctggg
    15061 gctttcactg tcagaaatac aataatctag aatcagacca tggtacggtg aatattcaat
    15121 tcgtgctgaa aatcttcctt tgttatcatc aagttggtgc caaaattcac catgaaaaaa
    15181 gtattctttt gttagttcgt atttttttgt tgccatgtac tgctccattt gtttctgaga
    15241 atccttcaga atagtaaaaa tactactcaa aactcgtctt caagatctct ggctatctgc
    15301 tcattctcat gatgctcaac agcatcactg atcaaattca tcatgttatt aagaaactcc
    15361 tctacaactt taaacgcatc attgagcgta gctgtattga atgtatgtat cttttcatcc
    15421 gttgttttcg ttttcccgtt acgatgaact atgtcatgcc tcagtttcat caattcattg
    15481 atatttttga gtggaaaacg tggatattgc ttaggttgca ggactgcttt atatatttca
    15541 actaccagtt ggattctatg ataaagaata tctgaaaggt actcctgtac atatttgttt
    15601 gcattagact ctttatttat caattcagac agagagatat tctttgcttt cagttcattt
    15661 atgtttctta tggcattttc tacatatctg ttatgtgaca atacaacgct tttaatcatt
    15721 tcgctaagac agttttccat tattgtcaca atgtatgcga ttttcatttt tatgaaaact
    15781 tcacttgtag attgttttcc ctcttctttt atttgttcta aaaggtttat tgctatttta
    15841 taaatttctg tatgtggatg ctgttttaac catttttcgg tttcatattc ttccatagcc
    15901 atatcagaaa gaaaatcctg atactcgtca tagtcctttt caagtaactg ccattcttca
    15961 gaatcttcct caagatttgg gctggaaagc cgctctcgta tccattcctc acggcgactt
    16021 tcctcgactt gtataaccca atcttttgtt gttcccacag taccttcctt atgttcattg
    16081 ctaaatatca atagtcactt tatcggagac tacgtgaaaa atcctatctt ttccccctgc
    16141 ctttttatta gatttatagc tcatatccaa actcacccct tatagaacgt acactggata
    16201 atgataccgg ttatacatcg catatttgcc agtcagcttg cttaattttt catcggctgc
    16261 agcatcagag cattttccag atgacagcta taatgaaaat gattgccaga ccggcgagaa
    16321 agcagagcga aagcgggatt ttgtccttca gtgtcaagtt tagttcttct tcaacccatg
    16381 ttttctcaaa ttcacagtaa aatttataat gtgcgtacca gttacttccg aagatctgtt
    16441 tgctgatgac cgtgccgtaa aaccgggaca tgatagcgtc gatggattgt tgtgtatgct
    16501 gatagtcaaa gtttgcatcg ctttttacgt tgcggtaaat aacttcaagt tcattaaaaa
    16561 tttttgtctg ctcaatcccc gcactttcat aactctgctt ttccgagtca taaaactgaa
    16621 tgtacattgt agcaacacca aatataatta atatcgcgga aattacgttt gtggctagct
    16681 caggaataaa aagagcgaac acaccgacag caagagaaat aaagctaatc caaccggatg
    16741 ccttacaaat tagatcatag gtggcaaagt ttttcttagc gccaaatcca acgttataac
    16801 catgtctggc aattaatttc agcatctctg attgcgtcat aaaaacctca gtttatggga
    16861 acttcaatct gggcccgggc aaccacaacc tcatttttta tcacatagca ttctaccacg
    16921 tgatctccgc ggaagtcggt ggtctctttc tttgtacagt agccgccgtc cgaaacgatt
    16981 tgtccccgga tcatatttct tctgcgtgct tcgtcaccca cgttcagtac cttccacata
    17041 acggtgtagg gttcctctgc ctccatttct gtcaagtctg cccggaaaag gagagatttc
    17101 cgagcggaga ggcgaaatcc tctagtaagc atctctctca ggcttctggg agggaaacca
    17161 tcttgggtta cggtacagtc aaggttgagt gagtgcctga tatccaccgg atatttatcc
    17221 tctataaatt cttcggtatc gatccaggga gctgcatctg ccgcatgcga ttccagacca
    17281 aggctggctt ccataatacc caccttacgt ctggggaagg ctcgtccgaa cacttcgcgc
    17341 cagaggtcat tttcactggc agacccctca gcgtctaagg catcacaaca taattcatac
    17401 gcatgtttcg ctgcccggcc aaaccaggga gatttaacac gtacatactg attactgcct
    17461 aacgcgagat acctttccct tttctcctca tttgacagat attcaaaaaa gtctctggcg
    17521 agggctccca ggctgccatt tccggtattg tcataatctg atgttgatga aagaaatcga
    17581 tacgccagtg tatctatcag taagcccccc atattgacgc catgcaggtt cttccaggct
    17641 ctgatcattt tacacagctt gcgcagatta ttggatttat ctcgcgaaaa tgcggtcata
    17701 gccgcctttt cgtgaagagg ttttgtaatg cgccatgctc ccccattata gctttcaggg
    17761 aatttaaagc tgccatcatc ctgtctgaat acaggttgaa cctcgacata aaagttttta
    17821 aagatgatct gaacaaccag tctgtccttt ttcaccgtct ggtcaggata ttcttccgct
    17881 aaaatgtttc tgaacatctg taagcaatgc agcctgacca ttatccttac ggttataata
    17941 ttcatactga ttggggggca taatataaag catgtccaga tcggatatgc cttttattgc
    18001 agtatgtctg cctactgacc ccactttcaa gcggtttgct acccgggaac tcgttcctct
    18061 gaacgcaaga tttaatgact tggttatttt tcgatagtga tagctgatct tttttgcctg
    18121 tttaaggtca agattcaaat tttcaagaaa ttcgctgaac aattccctgc ggctcatggt
    18181 atttcctttg gtatgtgaaa cattagctta cattatcatt acaccttata aaaaccaaga
    18241 tcttgggagg tgagcgttct gcatggcttt atgacttaaa cttttacagg acggtctata
    18301 aaaataattc atgaagtcat ttctgctctg cctactactt cttcaatgaa gggggaaaaa
    18361 acggaagtac agctgaacga caagcacaaa aaaacccgac tggtaatttg gct
//
