LOCUS       NZ_CP150658            13163 bp    DNA     circular CON 09-APR-2024
DEFINITION  Staphylococcus aureus strain BSN214 plasmid pAH5667, complete
            sequence.
ACCESSION   NZ_CP150658
VERSION     NZ_CP150658.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637794
            Assembly: GCF_038024225.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN214 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN214
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP043386, CP092053, CP014442
            Genome Coverage                   :: 140.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038024225.1-RS_2024_04_09
            Annotation Date                   :: 04/09/2024 00:11:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,971
            CDSs (total)                      :: 2,889
            Genes (coding)                    :: 2,806
            CDSs (with protein)               :: 2,806
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 46 of 83
            Pseudo Genes (incomplete)         :: 42 of 83
            Pseudo Genes (internal stop)      :: 14 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP150658.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN214"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pAH5667"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2018-11-05"
                     /collected_by="Andrew D. Berti"
     gene            join(13143..13163,1..963)
                     /locus_tag="UXR45_RS14770"
                     /old_locus_tag="UXR45_14770"
     CDS             join(13143..13163,1..963)
                     /locus_tag="UXR45_RS14770"
                     /old_locus_tag="UXR45_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181107.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            <983..1272
                     /locus_tag="UXR45_RS14775"
                     /old_locus_tag="UXR45_14775"
                     /pseudo=""
     CDS             <983..1272
                     /locus_tag="UXR45_RS14775"
                     /old_locus_tag="UXR45_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            1819..1968
                     /locus_tag="UXR45_RS14780"
                     /old_locus_tag="UXR45_14780"
     CDS             1819..1968
                     /locus_tag="UXR45_RS14780"
                     /old_locus_tag="UXR45_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(2136..2828)
                     /locus_tag="UXR45_RS14785"
                     /old_locus_tag="UXR45_14785"
     CDS             complement(2136..2828)
                     /locus_tag="UXR45_RS14785"
                     /old_locus_tag="UXR45_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            2938..3132
                     /locus_tag="UXR45_RS14790"
                     /old_locus_tag="UXR45_14790"
     CDS             2938..3132
                     /locus_tag="UXR45_RS14790"
                     /old_locus_tag="UXR45_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            4248..4865
                     /gene="cadD"
                     /locus_tag="UXR45_RS14795"
                     /old_locus_tag="UXR45_14795"
     ARG             4249..4862
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4884..5231
                     /locus_tag="UXR45_RS14800"
                     /old_locus_tag="UXR45_14800"
     CDS             4884..5231
                     /locus_tag="UXR45_RS14800"
                     /old_locus_tag="UXR45_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            5388..5480
                     /locus_tag="UXR45_RS14805"
                     /old_locus_tag="UXR45_14805"
     CDS             5388..5480
                     /locus_tag="UXR45_RS14805"
                     /old_locus_tag="UXR45_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5652..6623)
                     /locus_tag="UXR45_RS14810"
                     /old_locus_tag="UXR45_14810"
     CDS             complement(5652..6623)
                     /locus_tag="UXR45_RS14810"
                     /old_locus_tag="UXR45_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            6747..7166
                     /locus_tag="UXR45_RS14815"
                     /old_locus_tag="UXR45_14815"
     CDS             6747..7166
                     /locus_tag="UXR45_RS14815"
                     /old_locus_tag="UXR45_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(7692..8537)
                     /gene="blaZ"
                     /locus_tag="UXR45_RS14820"
                     /old_locus_tag="UXR45_14820"
     ARG             complement(7696..8537)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8644..10401
                     /gene="blaR1"
                     /locus_tag="UXR45_RS14825"
                     /old_locus_tag="UXR45_14825"
     ARG             8645..10398
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            10391..10771
                     /gene="blaI"
                     /locus_tag="UXR45_RS14830"
                     /old_locus_tag="UXR45_14830"
     ARG             10392..10768
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            11035..11613
                     /locus_tag="UXR45_RS14835"
                     /old_locus_tag="UXR45_14835"
     CDS             11035..11613
                     /locus_tag="UXR45_RS14835"
                     /old_locus_tag="UXR45_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(11974..12717)
                     /locus_tag="UXR45_RS14840"
                     /old_locus_tag="UXR45_14840"
     CDS             complement(11974..12717)
                     /locus_tag="UXR45_RS14840"
                     /old_locus_tag="UXR45_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             complement(7693..8537)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          1329..1476
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          2..963
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          1095..1264
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.605"
                     /coverage="91.0"
                     /evalue="1.04e-65"
     Typing          complement(11975..12717)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcaagaaa attacaaaga aagattttat caaatcccta aagtattctt taccagtgaa
       61 aattataaaa atctaactaa tgatatgaaa atagcctatg ctatcttacg tgatcgctta
      121 aatttatcta ttaaaaatag ctgggttgat gaagatggta acatttactt tgtttattca
      181 aatgaaaagc taatggaaat cttaaattgt aaaaaagaaa agttaacaaa aataaaaaaa
      241 gggttagaaa acgatggtct actcattcaa aaacgacgtg gtttaaataa accaaatatt
      301 ctatatttaa tgaaacctat tgtcacagaa cgtgatattt acaaaataga aaaagaagaa
      361 aacgatgttg aaccttacgg cgagaaggaa gttcgaaaat cgaacgtcca gaagttcgaa
      421 aatcgaacgt ccagaagttc gaaaatcgaa cgtccagaag ttcgaaaatc gaacactaat
      481 gatactgact ttaatgacat tgactttaat gatactgaga ataatgatat gaatgacttg
      541 aatgatataa agtacaaaaa tgaaattagt aatcattcaa atcattcaaa tcaatttaca
      601 cataattttg atgataaaga aatgctattg caagaattcc cagaacaact aactaattat
      661 ttattaaaat atgattatag agatttagaa atcataaagg ctgttattct taaagcaaaa
      721 aaatcattta attcaaggca tgaagatatg cattacatgt tagaggacat tgaggatgaa
      781 atattaactt ctttaaaaag acttaaaaaa gctatacatg ataggggagt taaaggccaa
      841 aaggaaacaa tcaagtcaat gcaagcttat ttaatgcaaa caattctaac tgaacttgaa
      901 gaaacacatg ccctttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa
      961 taattcaaat gattttataa cactcgaaga gacatcaaaa tattccgtta aatcatctga
     1021 ctttttaact gatgacgacg aaaaaaatca agaaattgta agtgatttag aaaaaggttt
     1081 gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa caaaagcata aaattttaaa
     1141 cttagacgat gccgaagatg gtaatttgat taatacaagt gatgaagata aaacgacaga
     1201 cgaagaaaaa aagcacattc aattaccgca atttggaatt tcgaaaagca aaattattat
     1261 ttaagacatt agtgataact gatgtctttt tttattgttt ttctgtaaaa aagtgctgtc
     1321 ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt gtgacactac agctttgtat
     1381 gatatcactt taaaataact taaaaccctt ggaatgtctg gccttgccag atctatcatt
     1441 tttgaatgat agcaaatttc ccttatgctc ttacggagat tttagagtta aattaaaatt
     1501 tctaagtttt tgataagaaa agccctgcag gaatttagaa aaacatgggg aagttttaaa
     1561 gtattttatg ctaattttta aatttgcatg taaatgggca gtatctaaaa aattagtcac
     1621 tgattttgct gaaatttttg tttgtgaaat tagcatatat ttatttggct catatttgcg
     1681 ttttaagaga ctgtatagat ttttaagtac aaaacctata tgatttgcct ctaagtcttc
     1741 aaaatgcctc ttaaaatcca aaataaaagt gtttaatatt gtaataagga ttgctatttt
     1801 tataaaagga gagatatcat gaagattaaa actttcgcta cttttactca tcgtcaatta
     1861 gataaacaag ttaataggtt tttaaagaat gatattcatg tattagaaat tcaaacacac
     1921 agtaattggt tatatatttc agctattgtt ttttatgagg ataaataagg aacatcgtat
     1981 ttatgccgaa aaaatttatt gatgttgaga agaaccctta actaaacttg cagacgaatg
     2041 tcggcatagc gtgagctatt aagccgacca ttcgacaagt tttgggattg ttaagggttc
     2101 cgaggctcaa cgtcaataaa acaattggaa taaaatcaac ctacagagca tccagctgca
     2161 aatgcagttc cgcaaactgc ttcacataca ccgccggcta ttggatttac gattgcccat
     2221 actccacagt agtgagcaca tgcataaagt ccagccatac tacaagcgaa tgctttgccg
     2281 ttccatttga atcctgcata tgttaccggt ctgtttttat caactttttc agccttatat
     2341 tcaacgtaat ttttgttaat aagtattttt ccctctttgt tttcagtcat acttcttttt
     2401 tcggcaatga attttgtgat ttttttgttg tagttatcat atattgtttc agaaactaca
     2461 atttcttttg ttttttcatt tttataagcg ttattagcta tgtagccata tgcattttta
     2521 tcatttgctt ctgtagctag atattttaat ttagaatttt tgtcttctag ctttattttt
     2581 ttaaatcctt cttttttcat ttgcttttcg aatttttgca tatcttcatt tttattttgc
     2641 aattgttcta gaaaacttat ttctttcttg gtcatttgtg atacttcgat gtttttgctt
     2701 ttcaattttt ctttttgatt ttctagtaaa gaactttttt gttcttcatt tgcgtaagca
     2761 ttagaaaaat tcgcgctaaa taccagtagg aacaaggaaa ttattgaaaa taattttata
     2821 attttcattt gatacctcca agataataag atattttaat tgtataagta agcgctttat
     2881 aaaatcaata tttattttaa tatatttata aacatatttt tgaaaggagt ctattttatg
     2941 aatgaaaatt tagaaaaata tataaaaagt ttgccacttt taggtttgtt aattagtatt
     3001 ttgttaattg ttttattctt ttttatttta gatgttgatg aagatttatt cgtggttttt
     3061 ttatactgtt tattgccttt gatagttaat ttgtctattt atggttttta tgtttttgtt
     3121 aaaagaaagt aagattatga ataattttgt ttttaatttg tatgctttga gggggttgga
     3181 aaacggtagg tgtatagatt agatttcttg tttttttgaa acgagtgtga acgagtttct
     3241 tcttgtcttg atttttgata aaaacgccct acaggaattt agaaaaacgt ggggaagttt
     3301 taaagaattt tatgctaatt tttaatttgc atgtaaatgg gcagtgtcta aaaatagtta
     3361 ttgtttttgc tcaaattttt gtttttgaaa atagcatata tttatttggc tcatatttgc
     3421 gttttaaaag catatataag ttttagatat aaaactatat gaatcacccc taaatcttta
     3481 aaatgccctc ttaaaattca aaataagggc ataaaaaata aaagctaccc cagataaagt
     3541 gaggtagctt tttctttcat acggtctatt tatttttctt tcgacgccgt atcgtcgatg
     3601 taattcaaaa aagttatggg ctataaatct acatcacttt ttcaagaatg tagtagcaat
     3661 attcaacgca aattaatttt tataactgct cggaatatct caagccgttt ctttaatttt
     3721 gaaataaaaa aatcgacgaa ggtcgattac gtttttgcac atgtcgattt atccgacgta
     3781 taagtgcgcc cttacgggat ttaactagat tataacgacg aattttagac ctgtaaagca
     3841 ataacaatta ggtctaccag gtagataaag tgtacaaagt agaaccgaaa aagttattta
     3901 ttttgaagat ttttcgtaga tataatttgc taataaactt cctaaaactg aataaagaaa
     3961 aatagttaaa ctcatgtaat cactccataa ttctgaagtg tctccatgtc tacgcataga
     4021 cacgcattta tgccgaaaaa atttattgat gttgagaaga acccttaact aaacttgcag
     4081 acgaatgtcg gcatagcgtg agctattaag ccgaccattc gacaagtttt gggattgtta
     4141 agggttccga ggctcaacgt caataaagca attggaataa agcacctatg attatttaaa
     4201 aatataagtt ataatacatt caaaaatatt tttgaatgag gtgtattatg attcaaacgg
     4261 ttgttgctgc tgctgttctt tatattgcta cagcagttga tttattagta attttattaa
     4321 tattttttgc tagagcaaag actagaaaag aatatagaga tatttatgtt ggtcaatatt
     4381 tagggtctat tattttaata ttagttagtt tgtttttagc ttttgtatta aattatgttc
     4441 cagaaaagtg gatattaggt ttattgggtt taataccaat ttatcttgga attaaagtgg
     4501 ctatttatga tgattgtgaa ggagaaaaga gagctaaaaa agaattgaat gaaaaaggat
     4561 tgtctaaatt agttggtacg gttgcaattg ttacgatagc aagttgtggc gccgataata
     4621 ttggtttatt tgttccgtat tttgtgacat taagtgttac taatttatta cttactctgt
     4681 ttgtcttttt aattttaatt ttcttcttgg tgtttactgc acaaaaatta gctaatattc
     4741 caggaattgg agaaattgtt gagaaattta gtcgttggat tatggctatt atttatatag
     4801 ctttaggttt atttattatt attgaaaatg acactattca aacaatttta ggatttatat
     4861 tttaatttag ggtgtgattt tatatgagtt atgaaaatac ttgtgatgtg atctgtgtac
     4921 atgaggataa agttaacaat gctttaagtt ttttagaaga tgataaatct aagaaattac
     4981 ttaacatttt agaaaaaatt tgtgatgaga agaaattgaa aatcatatta tctttgatta
     5041 aagaagatga gttgtgtgtt tgtgatattt ctttgatatt gaaaatgagt gttgcttcaa
     5101 cttcacatca tttaaggctt ttatataaaa atgaggtact tgatttttat aaagatggaa
     5161 agatggcata ttattttatt aaagacgatg aaataagaga gtttttctct aaaaatcatg
     5221 agggtttttg aaacgagttt cttcttgtct tgatactata aaaaacaaag ttatataaca
     5281 taagacgact tatcggaagt agatatgctt gtatattcac agatggtata ataatagagt
     5341 cgcctatctc tcaggcgtca attgacgaag agaggaggtg cattactttg ctaatcattt
     5401 tcgttcacat catagcacca gtcatcagtg gctgtgctgt tgcgtattat acttattggc
     5461 ttagtaaacg caacaaataa ttaataggcg actttataag ccagcatcga aaaaatcccc
     5521 acactactgc aatagtgtgg ggattggtgc attaattgtt aatcattttc gttatataaa
     5581 ttataacact ggttagtaga gaaatgcaaa atgattgaat taatagtaaa tgtattaatg
     5641 aatactcaca cttatacttt taaaatagtt cttcctaaat gttgtccttt taaaagctga
     5701 tctatcgttt tatagaattc ttcaaatcca atttcattgt atagaagtga atttcctata
     5761 ttccattcat ttgctaactt actccatata tcttcacgga tattatgatc aacatataca
     5821 gaatcaatgc caattaattt aatacctcta agaataaacg gtaatatatt agcgttaagt
     5881 gaatttcctg ctacatttcc acatacagta gcaacacctt gataatttag acgttttaaa
     5941 ccgtataatg ttacattact ccccactgta tctaagaaat aatcaaaaat acctttttta
     6001 agcggtttat tatcatcttc aattattatt acttgttttg cacctaattt ttttgcgatt
     6061 tctatttgct tttcttttct tacgatagca gtgatatttt catatccaat tttatgtaaa
     6121 atttgtatag caacactgcc cacaccacct gtagccccag acactaaaat ttcaggttgt
     6181 gatttgatat tcattccata tttttctagt tcaaaaattg ataatgctgc cgtaaaaccg
     6241 gctgtgccat ataccataga ctctttaata gacatttctt ggggaatagg aacaatccac
     6301 tctccaggca cttgtgcata ttctgaaaat ccacctgtat gtgtcacgcc aacatcataa
     6361 ccagtaacta atacattatc gccttcttta aacttatcat tcgaacttga ttcaacaata
     6421 cctgcaaaat ctataccagg aatcatagga taatctctaa ttacaccgcc tttatattgt
     6481 aaagctagca tatccttata atttattgaa gagtatacca cttttactaa tacatcaccc
     6541 ttcgataaat gagattcatc aactttttct actttataac taacatttga tgcacttttt
     6601 ctcactacta ccgcattgaa catttattag ccttctttca caattgtttt gtagaagtat
     6661 gtttttgttg caaaactatt ttcctacaaa acttatcatg atatgataag cgagtcaata
     6721 ctaatgattt aagagaggtt ttagttgtgg aattagataa ttgtataaat tatttgttga
     6781 gtacttctca gaatagagtt tttaaacatt ttaaaagttt attaagtccc tataatatta
     6841 ctccagcaga atatggtgtt ttgaattgct tgattaatag taatttgaat acacctaaag
     6901 cgattggtaa tactttaaat ttagaacctt caacaatatc tggaatatta gataagatgt
     6961 ccaaaaagga tctaataaca cgtcaaactg ataatgaaaa tagaagaact gtgctaataa
     7021 acagtacaaa taaagcaaaa gacttatggc caacattaga aaagaaagcc tatcaactta
     7081 ataatcatta cttcgctaat ttaactaatg aagaaactgt agtgtttaaa aagatattac
     7141 taaagataaa taaaactacg ttttaatttt tacgttataa aaaagttgaa gtgcgaatta
     7201 taatcgagta aatataaatg atagcttagc ttaattatat attaaagcca actatacttt
     7261 tattcccgaa aaacctacta tccaaatttc taagatactt tagcctctat aataaacata
     7321 ttgtaatata atttatgtat tatttaggtt taccctaatc gaacaataaa aaatacttgt
     7381 aaaatgctat tgtcactttg cttgatatat gagaattatt taaccttata aatgagaaaa
     7441 aagcaacgca ctttaaataa gatacgttgc tttttcgatt gatgaacacc tataattaaa
     7501 ctattcatct attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa
     7561 ttaagaaaat aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata
     7621 catgtcaacg ataatacaaa atataataca aactataaga tgttatcagt atttattatg
     7681 catttagaat attaaaattc cttcattaca ctcttggcgg tttcacttat caacttatca
     7741 tttggcttat cacttttatt gtctttattc gtaaaaatga ctaaaacaat aggttcagat
     7801 tggcccttag gataaacaaa agcaacatca tttctagaag catatgttat tgcttgacca
     7861 cttttatcag caaccttata gtcttttgga acaccatctt taattaaagt gtctccgttt
     7921 ttattattaa acattaaatc aagtaagaaa tttttatttt ttttgcttaa ttttccattt
     7981 gcgataagtt tatttaaagt cttgccgaaa gcagcaggcg ttgaagtatc ttttttgctc
     8041 tttggtgaat agtaatttaa ttctatctca tatctaactg gatttgttac tttatctcct
     8101 agttctttta gacgttgttt aacttttttg attccaccga tttcttttat aattttattg
     8161 tttgctgtat tatcactata tgtcattgaa gcctcaataa gttcttttaa agtgatatct
     8221 tttcctacat atttttctaa aataggagaa taagcaacta tatcatcttt gttaatatgt
     8281 acttttttat ttaacttatt ataaggtact tgttctaaca aaatagcact atttatcgct
     8341 tttgaagtcg aagcataggc aaatctctta tctgaattaa attttacttc cttaccactt
     8401 ttagtattta aagcataaac accaatatga gcattatatt ttttttctaa atcatttaac
     8461 tctttggcat gtgaactgtt tgaattacat gcacttaaaa ctaaagcaat tacaattaaa
     8521 aatattaact ttttcaaaat aaaccctccg atattacagt tgtaattttt ataaatcaat
     8581 aatattacaa ttgtaatatt gatgtcaatt gtttattata aatatttgaa agaaggtgtc
     8641 aaaatggcta aattactaat aatgagcgta gtaagctttt gttttatatt tctattgtta
     8701 gtatttttta ggtacatatt aaaacgctat tttaattata gtttaaatta taaagtttgg
     8761 tatctaacta tgcttgctgg attaattcct tttatcccaa ttaaattctc ttttataaaa
     8821 tttaataatg tgaataatca agcgcccaca gttgaaagta agtcacacga tttgaaccat
     8881 aacataaata ccaccaaacc tattcaagag ttcacaacag atatccataa gtttaattgg
     8941 gattcaattg ataatatctg cacagttatt tggatagttt tagttattat tttaagtttt
     9001 aaatttttga aagccttatt atatcttaaa tatttaaaga aacagtcact ttatctaaac
     9061 gaaaatgaaa aaaataaaat agatacgata cttttcaacc atcaatataa aaaaaatatt
     9121 gtgattcgaa aagcagaggc tattcaatct ccaataactt tttggtatgg gaaatatatt
     9181 attttgattc ctagttcata ttttaaaagt gtaattgata aaagactaaa atatataata
     9241 ctgcatgaat atgctcatgc taaaaataga gatactttac atttaattat ctttaatatc
     9301 ttctctatta ttatgagtta caatccatta gtacatattg taaaaagaaa gataattcat
     9361 gacaatgaag tagaagccga tagatttgtc cttaataata ttaacaaaaa tgaatttaaa
     9421 acttatgcgg aatctattat ggactccgta ttaaaaaccc cattttccaa caaaaatata
     9481 ttaagccatt catttaatgg taaaaaatca ttactcaaaa gtagattaat taatataaaa
     9541 gaagccgatc tgaaaaagca gtcaaaatta attctaattt ttatttgtat ttttactttt
     9601 tttatcatga taattcaaag ccagttttta atggggcaat ccttaactga ttataattat
     9661 aaaaagccct tacaaagcga ttaccaaatt ttagatgaaa gtaaaaattt tggttcaaat
     9721 agtggatctt tcgttatgta cagcatgaag aaagacaaat actatattta taatgaaaaa
     9781 gaaagtagaa aaagatattc tcctgattca acttacaaaa tttatttagc actgtttgga
     9841 cttgaccgtc atattataag tgataaaaat tcacgtatgt catggaatca taaacattat
     9901 cttttcgagt cttggaataa ggaacaagat ttaaatacag caatgcaaaa ttcagttaat
     9961 tggtacttcg aacgtattag taatcaaata ccaaagaact atactgcggc tcaactcaag
    10021 caattaaatt atggtaatga aaatttggga agttataaaa gctattggat ggaagatagt
    10081 ttgaaaatat ctaatcttga acaagtaata gtttttaaaa atatgatgga acaaaataac
    10141 cattttagta aaaaagcaaa gaatcaatta tcttcttcat tattgattaa gaaaaatgaa
    10201 aagtatgaac tgtatgggaa aacaggtaca ggtatagtaa acgggaagta taataatggg
    10261 tggtttgtag gttacgtaat tacaaatcat gataagtatt attttgctac acatttatca
    10321 gatggaaacc cgtctgggaa aaatgctgaa ttaataagtg aaaaaatatt aaaaggaatg
    10381 ggtgttttaa atgaccaata agcaagttga aatatctatg gctgaatggg atgttatgaa
    10441 tataatatgg gataaaaaat cagtatcagc taatgaaatt gtagttgaaa ttcaaaaata
    10501 taaagaagtt agtgataaaa cgattagaac attaatcaca agactatata aaaaagagat
    10561 tataaaacga tacaaatcag aaaatattta tttttactca tcaaatatta aagaagacga
    10621 tattaaaatg aaaactgcta aaacctttct taataaactg tatggagggg atatgaaaag
    10681 tttagtgctg aattttgcga aaaatgaaga attaaataac aaagaaattg aagaattgcg
    10741 agacatttta aatgatatta gtaaaaagta attagaggta tgtttcaaac ataatacata
    10801 tgtaaaccat acttgactct tattaaatta gttttgttaa tgacatttaa aatatattat
    10861 taaagatgag agtgtacaga aaataaccat tttctgtaca ctcttgtttt ttgtctaaat
    10921 aagtaagtat tacatagaat tatgtgttca aaaacccaac aaaaaatcta tgttcacaaa
    10981 agacctcgta atcaagttat ggtacactaa aggtgtatat aggaggtctg tattttgaaa
    11041 ataggttatg caagagtttc aactggatta caaaatttga atttacagga ggatcgatta
    11101 aatacatatg gttgtgaaaa gatatttaat gaccatatga gtggttcaaa aagtaagcga
    11161 cctggtttag ataaagcaat tgaatttgcg aggtcaggag atacgattgt tgtttggaga
    11221 ttagatagac taggacggaa catggaggat ttaatcacgt tagttaatga acttaacgaa
    11281 cgaggtgtga gttttcatag tttagaagaa aatataacga tggataaatc aagttctaca
    11341 ggacaattat tatttcattt attcgcagca tttgcagagt ttgaacgtaa tttaatttta
    11401 gagcgatcat cagccggtcg aattgcagca cgtgctagag gccgatatgg aggtagacct
    11461 gaaaaattaa ataaacaaga tttaaactta cttaagacac tttatgataa tggtacgcca
    11521 attaaaacaa ttgcagagca gtggcaagtt tcacgtacaa ctatttatcg ttatctcaat
    11581 aaattagagg caaaagagaa taaaaacaag tagagcaact aatctagctt actataatga
    11641 atctactaaa taattttata tatcattaac atacaaagac tttgattcaa tgtcactcga
    11701 tgtaaaagag acgttctgac agcgtctaaa atctcataat attttcctgt tatgagattt
    11761 taataataac acaagaaaaa agcgtacaaa ttcgtacgct tttttaatat aatacctgtt
    11821 tcactaatga acgtttataa cgcaatcatg atgagagtat taatacagtt aattatttga
    11881 aatactttag tttacataat cgatgattac cagaagttct cactatataa aagacactaa
    11941 aattttataa cttttagtgc ctaatcaatt attttagcta ccaaacaaac gactccaaaa
    12001 ccccttttta ggttccgttt cttcactctt ttcttctata tcactatcat tgtcatttag
    12061 attagctgga acgtcttctt tttttgcttc ttcaccttgt gtactttcgt cgctttgact
    12121 tccagatata tccttatatt gaatctctgt ttctttagtt tcttcctgat ctctgttaat
    12181 atttacagga ttagtagtat atgttttttc ttgtgcatca acgttttgtc tagtgttagt
    12241 agatgtatca aagggataat taaggtgttt ttctttttct aattgatttt ctaatttttg
    12301 aatcttttta ttactctcta atgccagaac ttgctgattt tcaagcaatt ttgatctttc
    12361 ttgcaactct tttctgtaaa attcaattgt tgtttcatga cgactttctt gtttttcaat
    12421 ttgattattt aaagtatcaa tctgattttt aaggatttct actatttgag catcgctttt
    12481 cgattcaaaa ttaatattat ccgacccttt tttttgtttg tcttcagtag tactttcaaa
    12541 accatatttc tttttatttt catttactct ttgaatgatt ttttctatat cggtatcttc
    12601 tttaataaac gaagtgtttt cctgttttat tgtctctata ttcaatcgct taatattatt
    12661 aaaaatcgtt tgcttactaa cccctaattc ttcactcaac tttttcacac ttttcatatc
    12721 atcaaccctt tataaaatgt ttataaaccc ttgataaaat attataagct tataatgctt
    12781 aataataaag tgattaataa ataaaaataa acatccatca acctttttat caagggttta
    12841 tgaaaggttt atgatattta tcaagggttt ataaaccctt gataaagcta tattttccct
    12901 tttataagtt tatttaaagt gctataataa acttaatata acaaaaaccc caactactgg
    12961 taatagtcgg ggtttggtaa taaaacgttt agaaactgtt tatttatata attttcgata
    13021 cttgtattat ataatgaata atatcttagt gcaagcacct aaatcattaa ataaatgtaa
    13081 agctaaataa aaacgtttta ttacctctta ccaaactaaa atatggaaag aggttttttg
    13141 tcatgcaaaa tcaatatttt aca
//
