LOCUS       NZ_CP150081             1906 bp    DNA     circular CON 23-APR-2024
DEFINITION  Escherichia coli strain ECSD228 plasmid pE228-1.9K, complete
            sequence.
ACCESSION   NZ_CP150081
VERSION     NZ_CP150081.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40597464
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Long,X., Li,J. and Tang,B.
  TITLE     The blaNDM-1 and mcr-1 co-exist with Escherichia coli strain
            isolated from environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Long,X., Li,J. and Tang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2024) Hangzhou Institute for Advanced Study,
            University of Chinese Academy of Sciences, No. 1 Xiangshan Branch
            Lane, Hangzhou, Zhejiang 310024, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: SPAdes v. 2023
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 200.0x
            Sequencing Technology        :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038397545.1-RS_2024_04_23
            Annotation Date              :: 04/23/2024 10:51:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP150081.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECSD228"
                     /db_xref="taxon:562"
                     /plasmid="pE228-1.9K"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2023"
     gene            complement(673..960)
                     /locus_tag="WL653_RS00005"
                     /old_locus_tag="WL653_26105"
     CDS             complement(673..960)
                     /locus_tag="WL653_RS00005"
                     /old_locus_tag="WL653_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072646593.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIKYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(967..1539)
                     /locus_tag="WL653_RS00010"
                     /old_locus_tag="WL653_26110"
     CDS             complement(967..1539)
                     /locus_tag="WL653_RS00010"
                     /old_locus_tag="WL653_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="WP_250649659.1"
                     /translation="MRNIIAGKDASMLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLN
                     PPDWSQVTKAGWNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLR
                     TIQYWIGDLESIGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVID
                     RKLRLMLSRREEFESSLKYSNHDSEQG"
                     /name="firmicute plasmid replication protein RepL"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.802"
                     /coverage="100.0"
                     /evalue="3.9e-116"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagggctt
       61 cgccccgaca ccccgcatca gtgcgtacgt gctcaatgtg gtttccggtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgttg tcggtgattc cgctgttgtt ctttcgggga taagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aaacggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggttgcta ggaacagcgt cagccccctg caaagcctat ttgcacggca
      421 caggcagcta ttcggttggt aggtggtcgt agaccattgg tgttacagcg atgggggtct
      481 ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc tatcggaacc gtggcggctc
      541 ggtcgttctg gagagcgggg aacaaaaaca cgcaagggat tcgaggcggc aaaagagcaa
      601 tgcaggatag acaatcatgg tcagaacgtt aacacgtagc ccctgattgt ccgcctgccg
      661 ctggctcaat ggtcattttt ttcgatattt atctagctca attacaggtg ctggagtttt
      721 tttctttgct attttttttg gcttttgtat tttttcatcg ttgctaacga caaacattat
      781 tttatttcca tcttcattga taacatcaac aagattaaat tctttatctt cacaattcat
      841 ataaagattt attcctgttg catatttaat accatattta ttaattaact catctattga
      901 actgcatatt tctaatttta gttcttctaa tctccaacgt gattcatatt taattctcat
      961 agtgcatcac ccctgctctg agtcatgatt agagtacttt aaggaacttt caaactcttc
     1021 acgcctagaa agcataagcc gcaatttacg atctataacc ccatcttttt gagttaatgt
     1081 ttttgcgtta aatgctgcat attttttagt attgttatat ccacgccata cctgataagg
     1141 atctaatgca taggcataat atatgcctac tggtatttta actattgcgc caatactttc
     1201 taaatcccca atccaatatt ggatcgttct taaactaaca ccgaaataat cagctaagaa
     1261 tttttgatca catacaacag ccccacatga tttatctata ttctgggcaa aaaaagaata
     1321 taaccgaaat gctctaggaa ctttttctga tatagcgtcc aacatattcc aaccagcctt
     1381 agtaacctgc gaccagtctg gaggattcaa ccgatctcgt ttttgcttag cgttcttgga
     1441 ttgatcttgt acctgatgat ctcgccgttt ttgctgttca aagtcttcaa attgctgttc
     1501 caacatactt gcatccttac ctgcaatgat atttcgcacc ttacgaaatt tatatcgtac
     1561 agtacgaaac aacacatgca aacatgtttg cgtatacaca tgcaaataac gtttcgtatg
     1621 atggtgtctt aagtcaatga tttttaaaga aatgtcaatt tttgctctaa agatctatca
     1681 aagcgttttt ttactgctca tgatttaaca ctacctaact gcttttgggg tgtcggggcg
     1741 cagccctgac ccagtcacgt agcgatagcg gagtgtatac tggcttactc ctctgcaagc
     1801 cttaccagct gtgatttttg ccagatttca cgctctcaac gttcaacagc tccatcatgg
     1861 ttttgccgtc cgttcaccac taaaacgcct cagaaatcgg gacagg
//
