LOCUS       NZ_CP150080             3373 bp    DNA     circular CON 23-APR-2024
DEFINITION  Escherichia coli strain ECSD228 plasmid pE228-3.2K, complete
            sequence.
ACCESSION   NZ_CP150080
VERSION     NZ_CP150080.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40597464
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Long,X., Li,J. and Tang,B.
  TITLE     The blaNDM-1 and mcr-1 co-exist with Escherichia coli strain
            isolated from environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Long,X., Li,J. and Tang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2024) Hangzhou Institute for Advanced Study,
            University of Chinese Academy of Sciences, No. 1 Xiangshan Branch
            Lane, Hangzhou, Zhejiang 310024, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038397535.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 10:53:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP150080.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECSD228"
                     /db_xref="taxon:562"
                     /plasmid="pE228-3.2K"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2023"
     gene            complement(848..1039)
                     /locus_tag="WL653_RS00005"
                     /old_locus_tag="WL653_26080"
     CDS             complement(848..1039)
                     /locus_tag="WL653_RS00005"
                     /old_locus_tag="WL653_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1103..1426
                     /locus_tag="WL653_RS00010"
                     /old_locus_tag="WL653_26085"
     CDS             1103..1426
                     /locus_tag="WL653_RS00010"
                     /old_locus_tag="WL653_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1635..>2072
                     /locus_tag="WL653_RS00015"
                     /old_locus_tag="WL653_26090"
                     /pseudo=""
     CDS             1635..>2072
                     /locus_tag="WL653_RS00015"
                     /old_locus_tag="WL653_26090"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2104..2664
                     /locus_tag="WL653_RS00020"
                     /old_locus_tag="WL653_26095"
     CDS             2104..2664
                     /locus_tag="WL653_RS00020"
                     /old_locus_tag="WL653_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2671..2904
                     /locus_tag="WL653_RS00025"
                     /old_locus_tag="WL653_26100"
     CDS             2671..2904
                     /locus_tag="WL653_RS00025"
                     /old_locus_tag="WL653_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3078..3212
                     /locus_tag="WL653_RS00030"
     CDS             3078..3212
                     /locus_tag="WL653_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484593.1"
                     /translation="MQHPENAFHAFLGIQPGENMVFLLFERKDVKNLVIALAIFRRWA"
                     /name="hypothetical protein"
     gene            3203..3334
                     /locus_tag="WL653_RS00035"
     CDS             3203..3334
                     /locus_tag="WL653_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484592.1"
                     /translation="MGVERKQLARKASCFGFIEVSDLLNVPVETVEIHNRNQFIQFA"
                     /name="hypothetical protein"
     Typing          684..767
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="95.0"
                     /evalue="4.11e-16"
     Typing          1417..2987
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.628"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcccaggaac cccgaaaaaa acctgttttt gcagatactt gcgtatttgc gagaatcgtt
       61 tgcggttggg ggagctgtga cgacggcgac cgcacgcgtg agagttccgc taccggcgca
      121 gagagtttca tcgacagcgg acacctgatg agttgccaca aaaaccgtat gtagcgcggc
      181 tagattggtt gagttagacc ttggcggcag ttcccggtat gtggtggcgt gacgggtgag
      241 atggtgcggc gaagacgcat acgtcacgca ggcgcgggtt cttccactga cgcgccccgt
      301 gatcgatggc cttggctccg tccggcatat cgagagcggc aaactacccg tcccttcact
      361 gggaggggtg agttgttgcc gttccctccg accttccacc atccagcata tcgcacgatc
      421 ccatgtgcac cgctactcct gataactcca gtgcaaaaaa aaaaacggat ctgcatcaca
      481 acgagagtga tcccaccgct cgccgcagcc ccagcgaccg aacgtagtga gcgagtgggc
      541 gaggaagcgg aagagtcaca gaggcaagca ttgtgcactt acgcgcctgt gccagtgtcc
      601 gcgtgtgtgt tgtttatcgg gcgcgctgtt tttacgcatc tgtgcggctt tttactcctg
      661 acatggtgca cttcccatcc actctgcacc gatgcaaaat gaggaaagcc agtatacaca
      721 tcgctatcgc tctgtgaact gggtcagggc tgcgccccga cacccgcact gacgcgccct
      781 ggcgggcttg tctgctcccg gcatccgctc acaggcaagc tgtgaacgtc tccgggagct
      841 gcatgtttca gttgctttca ccgtcatcgc cgaagcgtgc gaggcaactg cggtagagtt
      901 cgtcggcgtg gtcgtgaagt gattcgcaga tgtctgcctg ttcgtccgcg tccagatcgt
      961 tgagtttttc cagcagggtt agcgtctggt ttctgataaa gcgagccatg ttaagggctg
     1021 ttttttcctg tttggtcatg gtcttaaggc tccttgtatg ggggatttgt tagggggaat
     1081 gataccgatg aaacgcgaga agatgctcac gatacgggtt accgatgagg agcacgcccg
     1141 gttactggaa cgctgcgagg gtaaacgact ggcggaatgg atgcggcggg tctgtctggg
     1201 ggagccggtt gcacgcacag gaaaactgcc gacactggca ccgccattgc tgcggcatct
     1261 ggctgccatc gggaacaacc tgaaccagac agcccggaag gtgaacagcg gccagtggtc
     1321 ttcgattgac cgggttcatg tggtggccgc actgatggcc atcgagggtg aattgcgcca
     1381 gttgcggcag gctgtcaggg aacagggggt acgggatgat agttaaattt catgccaggg
     1441 ggaaaggcgg tggaagtggc ccggtcgatt acctgctggg gcgggagcgt aaccgcgaag
     1501 gcgcaacggt gctccggggt aacccggaag aagtgcggga actcattgat tccacgccat
     1561 tttcgaagaa atacacgtca ggcgttctgt cgttcgcgga gaaggagcta ccaccgggtg
     1621 aacgcgaaag agtgatgacg agctttgagc gtgttcttat gcccggtctc gaaaaagacc
     1681 agtacagcat tctctgggtg gaacaccagg ataaggggcg gctcgaactg aattttgtca
     1741 tcccgaacat ggagctacag agcggtaagc gcctccagcc gtactacgac cgtgcggaca
     1801 ggccgaggat aaatgcctgg cagacgctgg ttaatcatca ttacgggctg cgtgacccga
     1861 acgcaccgga gaacagacgg acgcttgtca cgccaaataa tctgccaaaa gcgaaacagg
     1921 aggccgcaga agcgattaca cggggtcttg aggctcttta ccatgccgga gcaataaaaa
     1981 cgcgtcagga tgtcacagag gcgcttacag cggcaggttt tgaggtggtg aggacaacca
     2041 aaagcagcat cagtattgcc gacccggagg gggggcggaa tctcaggcta aagggggcgc
     2101 tgtatgagca atcttttaca aatgggaaca gacttcgaga agaaactgaa agagcgagcc
     2161 gcttctaccg agaacatgct gaacagcgag ttcaggaagc tcgaagagtc tgtagacaag
     2221 gcattgagct taaacaggca gaaaatcaga gacgctatca gcgaacatac aacatccgtg
     2281 aaaaagcaac tggatacgct gagcaccacc gtgagcatgc agttcagcac cacagaggcc
     2341 gaactgtccc ggcagcagaa aaaactgctc tggcgggtga tcaaagggcg gatactgttt
     2401 ccggcgctga ccgcgctcag cgtcacgggc gggattttcc tggggtgctg ggggctgatg
     2461 gagtggcagg aaagcaagat tgcgaaaaat atcctgacta tcagagagca ggaaaacacg
     2521 ctggcgaaac tcgaggcaaa aacctggggg gtgacgttcg tcaatggcga gaacgggaaa
     2581 tttctggtgt tgccggacgg ggtgaaaggg gaaaacacct ggacggtggg cgacaagaac
     2641 gccgtgaggc tggtcaggga gtaaacgagt atgacggaac tggaagggca tttgctgaac
     2701 gccttagagc atttgcaaca ggactatatg cggcggctga acgagtggga gagcgccttc
     2761 gcggaattgc agaagatgca cgaggttacg caacggaaca acgcgatact gaacgagcgc
     2821 gtcgtgatat tgagtcagca ggtgcagaga ttagccgggc aagtggatcg cttgagtcgg
     2881 ttatttatcg cgaacaacag ctaagggatg aacaggagcg tgcccggtac agacatgagg
     2941 tacaggaacg cgcggcacgg gttcggagtg ggccatcaat ggagatgtga ccggagaagt
     3001 gacgaaatct tgccattttg acccgttcga cggctggggc gagcgaaaca aatcgtgtgc
     3061 cgtatttgtg catagctatg caacatcctg aaaacgcatt tcacgcgttt ttggggattc
     3121 agccagggga aaacatggtt tttttacttt ttgagcgtaa agacgtgaaa aatctcgtta
     3181 ttgcacttgc gatctttcgt cggtgggcgt agaacgaaaa cagctcgcca ggaaggcgag
     3241 ctgtttcggg ttcattgagg tctctgacct cctcaatgtt cctgtggaaa ctgttgagat
     3301 ccacaacaga aaccagttca ttcagtttgc atgagccttc tgtattcttc aagagttttt
     3361 caacagcaca gtc
//
