LOCUS       NZ_CP150079             3392 bp    DNA     circular CON 23-APR-2024
DEFINITION  Escherichia coli strain ECSD228 plasmid pE228-3.1K, complete
            sequence.
ACCESSION   NZ_CP150079
VERSION     NZ_CP150079.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40597464
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Long,X., Li,J. and Tang,B.
  TITLE     The blaNDM-1 and mcr-1 co-exist with Escherichia coli strain
            isolated from environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Long,X., Li,J. and Tang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2024) Hangzhou Institute for Advanced Study,
            University of Chinese Academy of Sciences, No. 1 Xiangshan Branch
            Lane, Hangzhou, Zhejiang 310024, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038397525.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 10:50:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP150079.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECSD228"
                     /db_xref="taxon:562"
                     /plasmid="pE228-3.1K"
                     /geo_loc_name="China:Shandong"
                     /collection_date="2023"
     gene            join(3356..3392,1..170)
                     /locus_tag="WL653_RS00005"
     CDS             join(3356..3392,1..170)
                     /locus_tag="WL653_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341875502.1"
                     /translation="MITDVWKYRGKSILDEELSLIRSGFMRADERTVTTSVYLALIAGT
                     VAEVSALFLIIVKSVFKNNNNDI"
                     /name="hypothetical protein"
     gene            complement(419..532)
                     /locus_tag="WL653_RS00010"
                     /old_locus_tag="WL653_26050"
     CDS             complement(419..532)
                     /locus_tag="WL653_RS00010"
                     /old_locus_tag="WL653_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071940873.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPQAAATGN"
                     /name="replication initiation protein"
     gene            832..1050
                     /locus_tag="WL653_RS00015"
                     /old_locus_tag="WL653_26055"
     CDS             832..1050
                     /locus_tag="WL653_RS00015"
                     /old_locus_tag="WL653_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071940875.1"
                     /translation="MFFPALSPDSVDNCITAFECAGTARRSRTTERSESVSEEAEECRK
                     LHFLLTHLCGISHRLWCTFHTIRTDAA"
                     /name="hypothetical protein"
     gene            complement(1197..1391)
                     /locus_tag="WL653_RS00020"
                     /old_locus_tag="WL653_26060"
     CDS             complement(1197..1391)
                     /locus_tag="WL653_RS00020"
                     /old_locus_tag="WL653_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_052923731.1"
                     /translation="MNKQQQTALNMARFIKSQSLALLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1561..2760)
                     /locus_tag="WL653_RS00025"
                     /old_locus_tag="WL653_26065"
     CDS             complement(1561..2760)
                     /locus_tag="WL653_RS00025"
                     /old_locus_tag="WL653_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000948429.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     gene            complement(2770..2958)
                     /locus_tag="WL653_RS00030"
                     /old_locus_tag="WL653_26070"
     CDS             complement(2770..2958)
                     /locus_tag="WL653_RS00030"
                     /old_locus_tag="WL653_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957857.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            <3073..3381
                     /locus_tag="WL653_RS00035"
                     /old_locus_tag="WL653_26075"
                     /pseudo=""
     CDS             <3073..3381
                     /locus_tag="WL653_RS00035"
                     /old_locus_tag="WL653_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /name="integrase core domain-containing protein"
     Typing          1030..1114
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(461..1004)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcttgatga agaactttcg ctcataagaa gtggctttat gcgggcggat gaaaggacgg
       61 taactactag tgtttacttg gctttgattg caggaacagt agcagaggtg tcagctttat
      121 tcttgattat tgtaaagtca gtttttaaga ataataacaa tgatatatag atttaacttc
      181 ggccacctct ggtgaagtta cgcatgtatt tggtcaatct atcttgagat cactctaatc
      241 aaaatgtaat ctcttgaggt tgaaacgaaa aaaccgcctt ggcgggcggt tttttcgaag
      301 gttctaagag ttggcgctct ttgaaccaag gtcactggct tggaggagca cagtcaccaa
      361 atctgttctt tcagtttagc cttaacaggc gcatgacttc aagacaaact cctctaaatc
      421 agttaccagt ggctgctgcc tgtggcgata agtcgtgtct taccgggttg gactcaagac
      481 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      541 gcttggagcg aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg
      601 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      661 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      721 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      781 ggaaaaacgc cagcggcgcg gcctttcgtt ggcctttgcc tttttgctca catgttcttt
      841 ccggccttat cccctgattc tgtggataac tgtattaccg cctttgagtg tgctggcacc
      901 gctcgccgca gccgaacgac tgagcgtagc gagtcagtga gcgaggaagc ggaagagtgt
      961 cggaagttgc attttctcct tacgcatctg tgcggcattt cacatcggct atggtgcact
     1021 tttcatacaa tccgcactga tgccgcatag ttaagccagt atacactccg ctatcgctac
     1081 gtgactgggt catggctgcg ccccgacacc cgctaaaacc ccctgacgcg ccctgacggg
     1141 cttgtctgct gccgaaaacg ctcgcggaca agccgtgagc gcctccgtca gcttcgttat
     1201 gttcctgttt cactttcggc ctcaaagcga acctggatgc tgttctggag ttcttccgcg
     1261 agttcgtgca gtcgttcgca catggccgcc tgttcgtcgg catccagagc atccagtttt
     1321 tcaagcagtg ccaggctctg gcttttgata aatcgcgcca tattcagcgc agtttgttgc
     1381 tgtttgttca cgtataggaa attgaaaaac cagtcgatac cattgtccac gccttatgcc
     1441 acgtgagcct ttctgctttt cccaccactc cggtggtttc cctctctgta tggtcgattt
     1501 ttataccgta acaggctgtt ttttatactt tcaccgattc ccgggcacac ttagcctgtg
     1561 tcaggccggc atatacctca gtagactgat atcccgcgcg tttttcttca gccctgatac
     1621 attcagcccc gcaatggccc ggcggtatac catccggcag ccacacagca cgcattcgaa
     1681 cgggtcacga ctcaggaact gtttcaccat ttgtgcatag cacactttcg ccactggttc
     1741 cggtttatcc atccccagtg cacggtacac ctgcggcagc ttctctccac acacacggtt
     1801 ggcaaggaac ccgaagtacc tcaccatctt aaaaaacttc tccgggatgt gctgtttcag
     1861 cctcgcgacc agctcacgct gtgtcagcgt ttccgtcgcc gtttctcccg ttttgtggtc
     1921 caggtaacgg aagctcaggc ttgcccctcc gttgtaatga gccagtcggg aagccgctat
     1981 tggtggcttc ttcagataac gacccaggta gcgtgccgta ttccgccctc cggctgtctt
     2041 cttcgacatg tacacatgcc agtattttcc gccggatttc agcaccaggc ttctccactg
     2101 tgattccgtc gtgatatgtg acaacgactc cggcattgcc atcccctctg accacgcttt
     2161 cagaagcagc tgccgcatat tccacatcca ccgtgaacgc atcgcgtctt tcaggaagct
     2221 cagctttttc cactgaccat gcttattcag acctccacag gttacagaca catgtacatg
     2281 cggatgccag ttgagacgac ggccatacgt gtggatggcg cagaagatac cgggttccag
     2341 cccccgtttt cgggcggcat acagcagatt ctccaccgcc agacggcaca cgtcattcag
     2401 cagccagcgg ttgctttcga acaccggcca cagcgtgtcc ggcagggtga agaccagatg
     2461 tacccagtcg cagtcaggaa gacgattcag ctgtgttgct gtccacaggt ctgtggcctt
     2521 ctttccgcag gacgggcagg cacggctgcc gcatgagttg gtcaggtact ttacgtgctg
     2581 acagtccggg ttatcacacc cgaactcttt tacacccagt atccgtgtgc cgcaggccag
     2641 cattttggtg acggcttcaa cctcgatatc gcgcagaccg cccgcatcca ggaaggacgt
     2701 ccagcactgg ttggctgtga acagacgttt cagagggcgg ggagtaaaac cggacaacat
     2761 gaggatggat taaaccccgg tatcctgtag cagggtaagg gctgctgtat cgccacggcg
     2821 cttcaggatg gaggtcgtca ccatcgcagg tggtgggtcc atgggtttga gcattttcat
     2881 ccagtcccat tctcgttgtg agagcttctc agcaccaaca gagagcagaa ctgtatcgat
     2941 acttttgcgg gtcaacataa ggggatgagt ccttaataaa acaggtcctc agagcataca
     3001 tattctgcgg ggcgtgcggc aacaacagga cgaaccgggg cggttttcaa tttgcagccg
     3061 ccaggctgcc gtggttcatg ctaccgggct aatgaaacac gccagttcgc ccggatgttg
     3121 ggacttgaac cgaagaacac ggcggtgcgg agtccggaga gtaacggaat agcagagagc
     3181 ttcgtgaaaa cgataaagcg tgactacatc agtatcatgc ccaaaccaga cgggttaacg
     3241 gcagcaaaga accttgcaga ggcgttcgag cattataacg aatggcatcc gcatagtgcg
     3301 ctgggttatc gctcgccacg ggaatatctg cggcagcggg cttgtaatgg ggtaagtgat
     3361 aacagatgtc tggaaatata ggggcaaatc ca
//
