LOCUS       NZ_CP150009             4510 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2024CK-00033 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP150009
VERSION     NZ_CP150009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39334914
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 4510)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 150.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038098235.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 12:49:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP150009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2024CK-00033"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2023-12-06"
     gene            797..988
                     /locus_tag="WLR76_RS00005"
                     /old_locus_tag="WLR76_28835"
     CDS             797..988
                     /locus_tag="WLR76_RS00005"
                     /old_locus_tag="WLR76_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            988..1398
                     /locus_tag="WLR76_RS00010"
                     /old_locus_tag="WLR76_28840"
     CDS             988..1398
                     /locus_tag="WLR76_RS00010"
                     /old_locus_tag="WLR76_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2185..2631
                     /locus_tag="WLR76_RS00015"
                     /old_locus_tag="WLR76_28845"
     CDS             2185..2631
                     /locus_tag="WLR76_RS00015"
                     /old_locus_tag="WLR76_28845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(3173..3466)
                     /locus_tag="WLR76_RS00020"
                     /old_locus_tag="WLR76_28850"
     CDS             complement(3173..3466)
                     /locus_tag="WLR76_RS00020"
                     /old_locus_tag="WLR76_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249640131.1"
                     /translation="MHKSTVLLCKKRIVGGHMALVRSGNKTKIFEADPKFNPVFDTQHK
                     PGDTCTYEGIYKCTGCGKEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="hypothetical protein"
     gene            3598..3918
                     /locus_tag="WLR76_RS00025"
                     /old_locus_tag="WLR76_28855"
     CDS             3598..3918
                     /locus_tag="WLR76_RS00025"
                     /old_locus_tag="WLR76_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4122..4478
                     /locus_tag="WLR76_RS00030"
                     /old_locus_tag="WLR76_28860"
     CDS             4122..4478
                     /locus_tag="WLR76_RS00030"
                     /old_locus_tag="WLR76_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     Typing          complement(269..678)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggcctctgt aatagtgtgc cttctttttt tgtaaccgct aaaggtcatc atcattttct
       61 tttacgagca cttgagatcc ggtttcggta cgcgtaatct cttgctctgt aaacggaaaa
      121 accgccttgc agggcggttt ttccgaaggt tcaaacactt gcaggtgatt gaaccaaggt
      181 aactggcttg gaggagcgca gtcaccaaaa cttgttcttt cagtttagcc ttaacaggcg
      241 catgacttca agactaactc ctctaaatca gttaccagtg gctgctgcca gtggcgcttt
      301 tgcatgtctt accgggttgg actcaagacg atagttaccg gataagacgc agcagtcgga
      361 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttcc cggaactgag
      421 tgtctggcgt ggaatgagat aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
      481 tggcaggaac aggagagcgc acgagggagc catcaggggg aaacgcctga tatctttata
      541 gtcctgtcgg gtttcgccac cactgatttg agcgtcaatt tctatgatgc ttgtcagggg
      601 ggcggagcct atggaaaaac ggccgctaag cggcctttcc ttctgcccat cacgcgtccg
      661 ctcggttttc tcgagcattt atacgcatag tcctaccgct cgccgcagcc gaacggcagg
      721 agcgtagcga ctgagtgagc gaggaagcgg aaaagagact ggttttacac tgagcactga
      781 cgctctgagg cttcttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat
      841 ccagaatcag tctctgctcc tgctggagaa gcttaacgag cttgatcttg atgcagaggc
      901 cgacctttgt gagaaactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag
      961 actggatgaa ttacaggatg gtaactgatg gccagagtgt ctattagtga agcggcacgg
     1021 cttgttaagg ttagtcgccc cacaatttac aagatgatta acagtgggaa gttaagttac
     1081 acctctgttg taaagcacgg gaagatcatt aaagttattg atacttcaga gcttattaga
     1141 gtttttggtt ctcttgatgg tgtcatcgat actgtaaaaa gtgacgcagg atctacaggt
     1201 gttaacagcg ttggtttaca tgatttacaa catagaattg ctttactaga agctgaaaac
     1261 gatggtctta aaggggctgt taaggctcga gatgagcata ttgattccct tcgtcaggct
     1321 atgcagcttc tggagcacaa gcacgaacct tcttcaccac ccgattcccc gtggtggaaa
     1381 ttctggaaaa aatcctgact ccgtcatgct tccgttttcc tctccggcca tcccggggag
     1441 ttcctgaacc ttcccaaaaa tcgccagatc tctgaacgct cctcaccact aaaaataccc
     1501 acaccccacg caaaattaaa attttgaaga taattcatta atatcagtta gttgtgggtt
     1561 attacattac gcagtattac tttctgtaag gtgaatccaa gtcgtcggcg attcgtgtac
     1621 gacttgccga cgactcgtca tagagtcatc gacgactcac ttaagactat cagtaactta
     1681 tgacgacttg actctagagt cgaatcgtca ttttggtgtt tttttgacct tggcgactct
     1741 agagtcaagt cacgagtcga atcgccggtg aatcgtgtgg tgagtcgtcc ggcgatttgc
     1801 ataacgtgat tgccggccgt cgacgaaccc gattcctgaa cgtgctgaaa accgtttttc
     1861 cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc
     1921 agtctggttt ggtgcgggtt ttgagttgtc tggcctgaat tcaggctgaa atccgccacc
     1981 agtcagtctg gtgcataaca attcatgccc gtaaaacgcc ctggagcgat tttgaggcgt
     2041 tttcaggggg taagacgtag gatttaatgg attacgcccc agaatcgttt ctagagcgtt
     2101 tgagcggtgc gcgtaatgag gcgttatggt aaataattgc tcgtgaaaac gttattaaca
     2161 tttttatctt atgaggtgat gtgagtgaac gataaatcag gctttagttt tatgaagatc
     2221 ggtgcaggtg caaaagtgac tggtttaaga atgaaaagga atgtgatggt tgttgatgat
     2281 ctgtctcacg tagagagctt tgttactgtt ggtgagcgag gcgaagttat taatctcgat
     2341 gcggatggga acgaactaca cacaccagac agttatcgtg aaaagttaaa aatagaacgc
     2401 ggtgaactcc aggcttcctt ggagaaagcc aaggaggaaa tcaactcaat agataacgat
     2461 ataacttcgc aaaagattaa gaaccagttg agtgctgcac tatctcaacc aatggatgca
     2521 gaaggtgagt ggaacactaa gagaatccta caaggggttt tagatttatc taaacaagtg
     2581 ggctgtggtg tactcacggc tgcgatcacg cattatcttg gagttagtta ataaactacc
     2641 tatgccccca tggtgggtat acaaaaacca tggggagcgg agcgaccttc gtctagctct
     2701 tttcttgtgt gtctcatctt gcaacggatt cgatttgtgt ggggttagtg tcagacttct
     2761 tggggtcagt ttggatatag agaattattg tacggtaagc ctgtgttttg aacaggctta
     2821 cctctcgcaa tgatgtactc ccgcgctata cgccgtttcc cgtgaatttt ccttaatcgg
     2881 ataacctaaa tgcgatacag aaagagcggt gacttaaaag cgatcgtagc tgaggtaaaa
     2941 tcaatccctt acgcatcaat attggttaaa taatgcgctt aacgtacaaa agtttccgat
     3001 atccaaactg acccctattt ggcggctttt cttattttct tgattttccc gcatctgaac
     3061 gtttctctcg gactcttcca ttattattac gatctcgtgg ctgagttccg cctcctggtt
     3121 tagggacgac gacatgcggg ttaagtggtc tacggtctgc cattttcata cctcactctg
     3181 cataaacaaa tagttgccag cggatgtccc cctgtccggg actatgttga tgatgatttt
     3241 gtggtggaag tgttttccct cctgcgatag ttacttcttt accgcaacct gtgcatttgt
     3301 aaattccttc atacgtgcac gtatcgccag gcttgtgttg tgtatcaaaa actgggttaa
     3361 atttcggatc tgcttcaaaa atcttggttt tatttcctga gcgaacaagc gccatatgac
     3421 ctccaactat tctttttttg cacagtagca ctgtgctttt gtgcatttta tatcattatg
     3481 gttttttgca caagtgcaca gtgctattgt gcatttctgg cgtttgttgt actatggtgg
     3541 tggtaaaagg tgatctagaa cttgccaagt tgcggcaaca tttgaggtaa aagaaaaatg
     3601 cagcaaaatc atgatagtga tgatgattgc cgcattcagt tccgagacga ttggctatac
     3661 cagttttata aatacggtaa agccaatcga aaaattccag ctacgattag tgtttcatta
     3721 ggtcgcaagc tggatatgct gaatgctgct gataaaattg gagatttacg gtctcccccg
     3781 gggaaccgat ttgaaaatct caaccctcca ttgacagggt attcatctat aagggtgaat
     3841 gataagtacc gcttgatatt taagtggggt gagaacggag tcgaggattt gtatctcgat
     3901 ccacatgatt accgataggt aacagaccgg aactgttatc ttacaaaaca agtaggtgag
     3961 gcatttcctc acctacgtta aaacgtcatc acaaataatg attaataaag taacgcgacg
     4021 taactatatt taagataatt actaatcgat acagctggta acagaagaaa tacttatctt
     4081 cctcatctaa cagagagaag cgaccaattc aggagtaaag catgtcccaa gcaaccagaa
     4141 aaccatcgac tgtaggagat attattcttt atgaatatct cgagccaagt ggtttaaaaa
     4201 tcaatgactt ggctgaaatg ttaaacgtgc atagaaattc tgcaagtgct ttggttaaca
     4261 ataataggaa actgtctacc gatatggcgt ttcggttagc taaagcattc gatactaccg
     4321 ctaagttctg gttaaatata cagcagtctg ttgatttgtg ggaggttacg agcagcccac
     4381 gccttcagga agaaatagaa agagtcactc ccgctcatga aattatagaa aggaagcaca
     4441 atgctgaatc tttggaaaaa aaacatgcgg tcgcatgatt tttttaaaaa aatttaacca
     4501 aactagaaga
//
