LOCUS       NZ_CP150008             5207 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2024CK-00033 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP150008
VERSION     NZ_CP150008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39334914
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 5207)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 150.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038098225.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 12:49:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP150008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2024CK-00033"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2023-12-06"
     gene            215..712
                     /locus_tag="WLR76_RS00005"
                     /old_locus_tag="WLR76_28805"
     CDS             215..712
                     /locus_tag="WLR76_RS00005"
                     /old_locus_tag="WLR76_28805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            719..934
                     /locus_tag="WLR76_RS00010"
                     /old_locus_tag="WLR76_28810"
     CDS             719..934
                     /locus_tag="WLR76_RS00010"
                     /old_locus_tag="WLR76_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1386..2636
                     /locus_tag="WLR76_RS00015"
                     /old_locus_tag="WLR76_28815"
     CDS             1386..2636
                     /locus_tag="WLR76_RS00015"
                     /old_locus_tag="WLR76_28815"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            2629..3438
                     /locus_tag="WLR76_RS00020"
                     /old_locus_tag="WLR76_28820"
     CDS             2629..3438
                     /locus_tag="WLR76_RS00020"
                     /old_locus_tag="WLR76_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(3658..3789)
                     /locus_tag="WLR76_RS00025"
                     /old_locus_tag="WLR76_28825"
     CDS             complement(3658..3789)
                     /locus_tag="WLR76_RS00025"
                     /old_locus_tag="WLR76_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            4403..4744
                     /locus_tag="WLR76_RS00030"
                     /old_locus_tag="WLR76_28830"
     CDS             4403..4744
                     /locus_tag="WLR76_RS00030"
                     /old_locus_tag="WLR76_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4144..4319
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          complement(3681..4161)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.786"
                     /coverage="92.0"
                     /evalue="0.0"
     Typing          2..525
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.714"
                     /coverage="86.0"
                     /evalue="1.9100000000000003e-79"
     Typing          4425..4738
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.04e-58"
     Typing          4735..5207
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.08e-97"
ORIGIN
        1 acgccttcag gactgccaga aacgaagcag gaagccgcag agacgattac acggggtttg
       61 ctgatgttag cctcatcagg agagctgaaa acgcgtgatg acgtcacagg ggcgctcaca
      121 gcggccggtt ttgaggtggt cagaaccaca aaaaacagta tcagcattgc cgacccggac
      181 ggggggcgaa atatccgact gaggggagcc atttatgagc agtcttttaa cggcctcgag
      241 ggacttcgag gagaaatcga aagcacagca gcagcttacc gaagagaggc tgaaagccgc
      301 attcagcaag cacgagaacg ttgtcaaatc ggaactgaac gcaagcgcga agagaatcaa
      361 cgacgccatc agcgcccacg aacaggggat gaacgcggcg atgcagtcga accggctgag
      421 cgtgctgcga atggtcgggc ggacgtggct gaccatcacc atggtgtcag ggctgctgtt
      481 cgccagcctg agcggggtgc tgtggtacca ggggaaccgg atagcggcga acctggcgga
      541 aatcaggcag cagagagata cgctgtcgaa actgcacatg cagacgtggg gcgtgaccta
      601 tctggagaac cgcaacggac ggtttctggt gctgccggag gggatgaaag ccgaaacggg
      661 ccggacggtg gacaacaaga cgcggaacgc ggtgaagctg gtacgggagt agagcagaat
      721 gacggaactg gaaaaacaat tgttgagcgc gttagagcag ctacagcagg actactcgag
      781 aaggctggac gagtgggaga gcgccttcga ggaatggcgg aagatgtctg gtcttatgca
      841 acgggagaac gcggcgctga gcgagcgcgt gtcgggcttg agcaggcagg tgcagagttt
      901 gagcgagcag ctgcgccggt tatcgacagg ctgaatgaga ttgagcaaca gcgatacggg
      961 caaacaacgg aaaaggaacc agagaagaca gcagatctgg taccggaacg gcaacgcaca
     1021 ttatctcatg gtgacaggga gtatggagac tgataaacgc ccgtctcagg tggcctgagc
     1081 gtgacgggga tcatggccat tttctgcgat ttcagcacac agagagtctt ttttaaccga
     1141 aatcaccggc tatgcagtga tgtgaataag tgatgcatat gcgtaaaata aatcaggtgc
     1201 gtttctggtg gttttcggtg tacttgccac caccttttgc ccgtcagcca tcagaaacgc
     1261 cctgagtgcc tccggcgcgg tgcgggtaag agatgttatg ttcaatcgaa atcagaaaaa
     1321 cgatcggtaa agcgatcgtt tttctgatag catcatgttg attttatata acgtgggtgt
     1381 aaagaatgac tagaccgatt gctgttgatt tatttgctgg agcgggtggg ctgagtctcg
     1441 gtttcgagca agctggcttt gatataatgg catcagttga aatagatcca atacattgct
     1501 ctgctcacaa atataacttt cctatgtgca aaacgatatg tagaagtgtt gttgatgtta
     1561 ccggagaata cgttagggaa gtttcaggta ttggtgagaa agagattgat gtagtgatcg
     1621 gaggcgctcc ctgccaaggc ttttcattaa taggaaaacg cgcgcttgat gatgatagaa
     1681 acaaactggt ctatcactat gtacggctgg ttcttgagct aaagcctaaa tactttgttt
     1741 ttgaaaatgt aaaagggttg acggttggta agcataagca gtttttagta gagataatgg
     1801 atgcatttaa agatggcggg tacgacgttg taagcgatta taaagtgcta aacgcagctg
     1861 attatggagt tcctcaagat cgccgtcgtc ttttcttaat ggggggaagg aaaggaattg
     1921 ctctgccatc ataccctgag ccattatcca ttagaacaac ggttggggac gctattgggg
     1981 atattcctga tgcggaaaaa tatcctgagc tatggggtag agattgggtt tatgctaaat
     2041 ttggtgagca atcactatac tcatcctatt tgcatggcgt aggaaaagat cctagtgatt
     2101 ttagctaccc aaggattttt aataaagaca aattaacgtg ttctttactg acagatcact
     2161 ctgacatttc tagagggcgg tttgttgaaa ctttgcctga cacggtagaa ccaatcagca
     2221 ggtttaaaaa gctctcgatg aatgggctat gtaatacttt aagggcagga acagcaagtg
     2281 acagaggtgc ttttacatct cctcgtccta tacatccgat ctatcctagg gtcatcacgg
     2341 tcagagaggc agcaagactg cattcatatc ctgattggtt cagatttcat gtgactaagt
     2401 ggcatggatt tagacaggtc ggtaatagtg ttcctcctat gctagctaga agtgttggct
     2461 tgcagattat gaaggttctt ggtgttacgc caaaaaaacc aacgaagaaa atcaaactcg
     2521 gtgatgaaag tttggttgct atttctatgt ccgatgctgc atctatgttt ggtgtgtcat
     2581 caaatgtaat tgctaagaga ataaaaaata gtaacaagga ggttgtttgt gagtaaggat
     2641 aaaaaagaaa ggtccaaaaa tagataccat cagttggtag aaaagatatt tttcgataaa
     2701 tatgttgatg gtgacttaga ggttttgttt gaaaggttag atataaacag tgctgcaaaa
     2761 gatttaggta tagaactccc atctaatgtt ggtgatgtta tatattcaat cagatttaga
     2821 acgcctatgc cagaaagtat tctgttgact cagccagaag gtaaggagtg gattattgag
     2881 ctggctggta ggtcaaagta tcgatttaga ttagtaacta ttagtaggat tttgccaaat
     2941 cctaatatgg tggcaataaa aattccagac aataccccgg agataattac aggttattct
     3001 ttagacgacg agcaggcatt gctggcgaag gtgagatata accggctaat agatatattt
     3061 ctaggtataa ccacatattc tttacagaat catttaagaa ctacggtgaa gggaattggt
     3121 caaatagaga tcgatgaact ttatgtaggt atagataaat acggttgcca ttatataata
     3181 ccagttcaag caaaaggtgg gacagatcaa atatcaatgg tgcaaacatc gcaagataca
     3241 gcgtggtgtg aacaaaaata cccaaatgtt agatgtagag ctatttccgc tcagtttgtt
     3301 tcgagcgatt tgattgcact gtttgagcta aagatagacg aaggacaagt gaaattgatt
     3361 gaggaaaggc attacaaact ggttccggta aaagagttag atagctctga catctcaaac
     3421 taccgcatgt ctgtataatt tctgagagga tcttcttgag atcctttcgt tctagccgta
     3481 atctcctaag ccgtaaacaa aaaaaccacc gctaccaacg gtggttcgtt tgccggaatt
     3541 tagagctacc aactctttct tccgaaggta actggctttc acggagcgca gataccaaaa
     3601 ctgtcctttc agtgtagccg tagtcaggcc accacttcaa gaactctgta gataactcta
     3661 ctccgctccg tgaatccgat taccagtggc tgctgccagt ggcgcttttg catgtctttt
     3721 cgggttggac tcaagacgat agttaccgga caaggcgcag cagtcggact gaacgggggg
     3781 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     3841 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     3901 gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt cctgtcgggt
     3961 ttcgccacca ctgatttgag cgtcagattt tgtgatgctt gtcagggggg cggagcctat
     4021 ggaaaaacgg caacgccgca tcctttttcc tgactttcct gacttgagcg cgatctcgtt
     4081 atctcgtcct tcgtgagctg gtaacgcccg ccgcagtctc acggcaggag cgcagcgacc
     4141 gagtgagcga ggaggcgtca tttcatcgtt acctgcattt tgcacttacg ccccgcagcc
     4201 gtattttccc ccttgccaca tgaagcactt ccccgaatct ccatctcgcc gtaacatact
     4261 aagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc ccgaaacccc
     4321 actgacgcgc ctgcggcttg tccaaccccg cgccgaccgg gaaaggcttt cccgtccgtc
     4381 acggcgttat caggaggcga ttctggcaga taaacgcaac aagatgctca cgatgtgggt
     4441 gacggaagac gaacaccggc ggttgcttga gcggtgcgac ggaaaacaac tggcggcgtg
     4501 gatgcgccag acctgccttg atgagaaacc gacacgcgcc ggcaggcttc cctcgatttc
     4561 gcccgcgctg ctgcgtcagc ttgccggcat gggcaataac ctgaaccaga tcgcccggaa
     4621 agtgaacgcc ggtggtgccg gtcatgaccg ggtgcagatc gtggcagcgc tgatggcgat
     4681 cgatgccggt ctggaacggt tgcggcatgc cgtactggaa aaagggccgg acgatgattg
     4741 ttaaattcca tccccggggt cgcggcgggg gcgccggtcc tgtggattat cttctgggta
     4801 aagaccggca gcgtgaaggt gccagcgtcc tgcaggggaa gccggaagaa gtccgggagc
     4861 tgattgatgc gtcgccgtat gtgaaaaaat atacctccgg ggtgctgtca ttcgcggagg
     4921 cggacctgcc gccgggtcag cgtgaaaaac tgatggccag ctttgagcgg gttctgatgc
     4981 ccggactcga taaagaccag tacagcattc tgtgggtgga acacgcagac aagggtcgtc
     5041 ttgagctgaa ttttctgatc cccaataccg aattactgac ggggaaacgc ctccagccgt
     5101 attacgatcg tgccgaccgg ccgcgcatcg atgcgtggca gacagtggtc aacggcaggc
     5161 tggggctaca tgatccgaac gacccggaga accggcgggc gctggtc
//
