LOCUS       NZ_CP150007             9294 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2024CK-00033 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP150007
VERSION     NZ_CP150007.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39334914
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 9294)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 150.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038098215.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 12:49:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP150007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2024CK-00033"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2023-12-06"
     gene            complement(706..855)
                     /locus_tag="WLR76_RS00005"
                     /old_locus_tag="WLR76_28750"
     CDS             complement(706..855)
                     /locus_tag="WLR76_RS00005"
                     /old_locus_tag="WLR76_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(940..1197)
                     /locus_tag="WLR76_RS00010"
                     /old_locus_tag="WLR76_28755"
     CDS             complement(940..1197)
                     /locus_tag="WLR76_RS00010"
                     /old_locus_tag="WLR76_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(1207..2892)
                     /locus_tag="WLR76_RS00015"
                     /old_locus_tag="WLR76_28760"
     CDS             complement(1207..2892)
                     /locus_tag="WLR76_RS00015"
                     /old_locus_tag="WLR76_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3295..3714
                     /locus_tag="WLR76_RS00020"
                     /old_locus_tag="WLR76_28765"
     CDS             3295..3714
                     /locus_tag="WLR76_RS00020"
                     /old_locus_tag="WLR76_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4020..4283
                     /locus_tag="WLR76_RS00025"
                     /old_locus_tag="WLR76_28770"
     CDS             4020..4283
                     /locus_tag="WLR76_RS00025"
                     /old_locus_tag="WLR76_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            4273..4572
                     /locus_tag="WLR76_RS00030"
                     /old_locus_tag="WLR76_28775"
     CDS             4273..4572
                     /locus_tag="WLR76_RS00030"
                     /old_locus_tag="WLR76_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4634..5011)
                     /locus_tag="WLR76_RS00035"
                     /old_locus_tag="WLR76_28780"
     CDS             complement(4634..5011)
                     /locus_tag="WLR76_RS00035"
                     /old_locus_tag="WLR76_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            5080..5259
                     /locus_tag="WLR76_RS00040"
                     /old_locus_tag="WLR76_28785"
     CDS             5080..5259
                     /locus_tag="WLR76_RS00040"
                     /old_locus_tag="WLR76_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5287..5817)
                     /locus_tag="WLR76_RS00045"
                     /old_locus_tag="WLR76_28790"
     CDS             complement(5287..5817)
                     /locus_tag="WLR76_RS00045"
                     /old_locus_tag="WLR76_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5824..6555)
                     /locus_tag="WLR76_RS00050"
                     /old_locus_tag="WLR76_28795"
     CDS             complement(5824..6555)
                     /locus_tag="WLR76_RS00050"
                     /old_locus_tag="WLR76_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(6555..8519)
                     /locus_tag="WLR76_RS00055"
                     /old_locus_tag="WLR76_28800"
     CDS             complement(6555..8519)
                     /locus_tag="WLR76_RS00055"
                     /old_locus_tag="WLR76_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7892
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          25..603
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5828..6555)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gtgaataagc aggggactaa catgtgaggc aaaacggcaa ggccacgcag gtggcgtttt
       61 tccataggct ccgccctcct gactgagttc acataaacag acgctttttc ggtgcatctg
      121 tgggagccgg tggctcaacc atgaatcagt caggagtggc gaaacccgac aggacttaaa
      181 gatcatcacc gtttccgatg aatcgctccc tcttgcgctc tcctgttcca accctgccgt
      241 ttcccggata cctgttccgc ctttctccct tgcgggaagt gtggcgcttt ctcatagctc
      301 acactctggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
      361 ccccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg
      421 gaaaagcacg caaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
      481 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag
      541 gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac
      601 tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
      661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat
      721 ccccgtcagt tcagaggagg acgatggtgc caccgttcca ccctgaacat cccggatata
      781 gtttgcctga catgcgacca gaagaaagcc tgaaaaaatc aatataaaaa gaaaaacaac
      841 ctttaccttt ttcatatacc ttccctgtat ttaaaaaccc cggtattcat ccctgaacac
      901 cggaagtctg tatttcaaat catccttgat aacttttgct taccagttac catcccggta
      961 aacaaaggat acaaagtaat caaatttact aatatcgatt acatttttaa agtgtggctg
     1021 taatatcgaa acccatgatt tttcaacatc aaaccaacca ttattaatat tatcctttaa
     1081 aggcatcccc agactttcaa tgacagaacc atcatcaccg aagtcttttg agtattcacc
     1141 acctttaaac tcttcggttt tcttatcaaa ccaatgaata tgtaatttaa gccccatatt
     1201 tacctcttaa agatattttt taatgtttcg ttttggatcc ggccctttaa cctgcttacc
     1261 cgtttttgga tcgaatgccc cgaggtgttc gccatcactg gcgcggtacc cttcaagctc
     1321 accgtgctgg gaatcccatt cataaatttt acgcttttta tcaccatacc atcgggcccg
     1381 cttaccacca ccaccttgtt taggggtttt aggtttaccc tctttcaact ctcccaaccc
     1441 cttaatatct tcagtcttgg gatcaggaaa ataatcatgg ccgtaatctt tagttccctt
     1501 acgaggtttt ttcttttcct catttaattt attttctgca tccttcgctt ttttctcctt
     1561 ttgtttccgg ctctccatcg ccccggaaag cgcagcctta tccctggata aggattcatt
     1621 catttcgttt aaagatgtct gggcagcttt taatttctca ttctgattct gaatatcctt
     1681 atcaagatag cgaacctgat cctgccaccc ggagccatat tcatgaggcg ggttataagt
     1741 cacaaaatca tctttatttg cctctgcctc ttttaccttt ttatcggctt cctgaacagc
     1801 agcccttgca ccggggatcg ccttctctgt cgatgcaatg cgactgttta aggtcgcaat
     1861 atttttatct tcagcatcca gctcagcttt cgctttatca tactctcttt tcagcccctc
     1921 ttccgggtga gcagcgtccc atgcctgctg gcgacgcttt tcttcctcca gacgctgttt
     1981 tacctgtgcc ggagtaagaa catccgtcac cgacacatag accggtttct gtccgctctc
     2041 tttcgggaaa cgaataacgg cttcacggga gttaccaccg gccgtaaaac cagccggacg
     2101 tgaatcacct ttttcagcaa taatgccttt tggcggggct ttcgcggccg gaacaccttt
     2161 aggtacgctc acctgcagag acgggagacc cggaatcgac acactgaata cccccggacg
     2221 ttttgtcggt ttcgcatcca ccacaggaac actcattggc cggccggtga caaccgcaat
     2281 atgctgccgc tcgtccttca caatatccgc cacacgttta gtgacgctga ccgtcgcctg
     2341 gtccagcggc agggagctga ccggcgtctc cgttaccgtg tctgccggca atgacgtcac
     2401 gatttttgac atcattttcg gatcatcttt tgctatctca gaaggcagca ctccgtaaat
     2461 cgcaatcccc cacagaccaa acttaaacgg acctttcagg gcagccagca catcagcaac
     2521 agcggccgac aacgcatcgc cggaaacgga taaagccagt ccttcagccc ccggagtggc
     2581 caaggcaggc agaccgaagg ccatcgcggt ggccgaagac tgtccgccac cagagctccc
     2641 gctatgattg ccgctgttac cgccattacc accgggttta ctcccgccgt taccaaactc
     2701 ccccgggtta tagttatgaa tatccccgtt aggtgtatgc gtcgtacccc agcccggccc
     2761 gctgtttgga tccgtaccac cacctgatga agggccacct ttacctgatg taccgttcac
     2821 attcccactg gcctgaccac cattgtgtcc cagacctgaa ttacccggac ctcgtccgtc
     2881 tccgccactc atatactttt cctctttgac ttttaaaaca ataaactaaa ataaaatact
     2941 gtacatataa ccactggttt tatatacagt ataaaaatta cgctgccttg tttttcatgt
     3001 caagtctgta aatttgcgtt tttgtgaaaa tgatcaaaaa aacagccact cacaccatgc
     3061 gcaatcccct ccagatgcaa caaaaaccag cacaaacggc gccgttctcc ggcctcagta
     3121 tgttttactg aaatcattat atgagatacc agaaagcccc ctccgggaca ggaaaagaaa
     3181 cggggggtct ggttcgggtt gttcggtcac tgaacacggt cagaaactga tgataagata
     3241 gcctgcggat atataactca atgagaaaaa agaattaaag gtaaggagac gctcatgaga
     3301 cgttccggca gcaaactgtg tcttatcgcc atgataacag cgacagcaac tctcactgcc
     3361 tgtgctccaa agggcagcgt ggaacagcat acccggcatt acgtatatgc ttcagatgac
     3421 ggttttgatc ccaatttttc cacccagaaa gctgacacaa cacgaatgat ggtgcctttt
     3481 ttccggcagt tctgggatat gggcgcaaag gataaagcga ccggaaaatc acggagtgat
     3541 gtgcagcaac gcattcagca gtttcacagt caggaatttt taaactcact ccaaggcaca
     3601 acacaatttg cgggtaccga ttaccgcagc aaagacctca caccgaaaaa atcaaggcta
     3661 ctggctgaca ccatttcagc tgtttatctc gatggctatg aaggcagaca gtaagtggat
     3721 ttaccataac gcgtcattac gcgcaccgct cagacgggct cagggcgttt ccggcggcta
     3781 cgggataaaa caggcaacaa cccccccgga aaactgccgg aaacgcgccc gataaatttt
     3841 atcctaatgc atgcctatgc acgcaactgc atggcatatg ttttgacgta aaatggatgc
     3901 cgtaagccct tttttaccga ctttctgctt catcagcctg actgacacga ccatattctt
     3961 tcagaccgcc ccctgctaaa cttccttcat attgccgcat taacccggga ggctccgcaa
     4021 tgagcaacac tacacactac gaaaacgcca actttcttcg ggaactggct gaaagcctgc
     4081 cccgcatact gccggaaggt ggcccggaca aagctgcgct gttgcagcga ctggctaacg
     4141 aagaactggc ccaggcagag tatgaagatc aggttcgcgc caaagtgacc gccgcccgtg
     4201 ctgacacgcg tccgggtatg accacggaac aactccgcca gcgcctgcat ggccgttatc
     4261 aggaactgcg cgatgccgta tgacgtggaa tggaagcagg gagccatcga ggatgtaacg
     4321 acgctgtttg actatatcgc ggaaaattca tccctgtggg atgcgcagaa cgttaccgac
     4381 cgggttctcg ctgcggcgga caaactggcc gacttcccgc gactctatga agtggactca
     4441 cgctatggcg aggacgttcg ccggatcagt ctgatgggac agcatgtcct ctatgatgtt
     4501 gatgaccaga ccagaaaagt ccgggttctg gccgtggtcg gccagcgcca gaatccccat
     4561 attgttctct aataaccggt gacggggacg caattcattg cgtggccgtc gccggttgga
     4621 caagcacagc gcgtcagcgt tttccccacg gccaccaccg gcgcggtgtt ccggccgggg
     4681 tctctgctgt ctccggtttg tgttccagca gtctcatgga ctgcatttcc tgccgtagcc
     4741 cggccacctc ctccctgagt gcttttatct ccgcgatcaa cgcgtcggag gccatttttt
     4801 cagtctcacc cgacagtctc aactcgtccc gctcgtgagt ctcatcctgt cttaactcac
     4861 cgtacacccg gataagctcg ctggtatcca ccacccggcc gccatcagct tccgtacggt
     4921 atgatacgcg gcctttcgcc atatcccgat acagggaact tcgggcttta cccgtcagtt
     4981 ccgtcgcctg tctcaccgtc acccacgcca ttgtctcacc ccgctatttt tgagacagta
     5041 tacctgacag gcgcacacca tgcccggaag gaaagaatca tgacaaaaca ggaaaaaaca
     5101 gccctcaata tggctaaatt tattaaggag cagtcgctgc tattgctcga taaactgaat
     5161 gagctggata tggatacccc ggccgacatg tgcgagcggc tacacgagga tgcggaaaca
     5221 ctctggcagg tgatgcggga taagctcggg gaggaataac agcaccggca tggccggtgc
     5281 tggcatttat tttttgccga acggccacca ccggcgggca ggtttgtcct ccggcagttc
     5341 aggtaccggc acgttaaaat agcggctgtt atattcccgc atcagctccg ctttcagctt
     5401 ctcgttcacc gcgctttccc cgtgccgctc actcaccacg tcaatcatcg cgttaatctg
     5461 ctccggtgag cacttcccgg catcaaccac ctgcacaccg ttctcgatgg cgaaccgctc
     5521 caccttgcga tagaggtaat cacacagctt cattcctgtg ggtttgttcc gttcccattg
     5581 cggattttta ccagtgaaaa agtacgtggc tttttcgtcg ctgttgagcc gctcgtcata
     5641 catcccgatg gatttcatca tcgccagatg ctgggcccgg tcgacaaaac agaaaacata
     5701 cgccccggtc tgccaggcga gcaccaccat atcttccaga tgctcgctgg tcggcatgat
     5761 gcagcgctcg ccgttccggt cctccgcgtc ttcaataaat accatgtaca gcgccataat
     5821 ccctcactcc gttgtcataa atttaaagca cgcaaacaac cgttcacgat ccagctctgc
     5881 cgttttgcgg gcctcgccgg tctgtgggtt cacgatttca tccaccgaca ggattgcccg
     5941 ctgcaccctg acctgcgtcg cccggtccgg gcagatgtac cagatttcat cgatgccgtt
     6001 agctttgcgg ttgaacagat acccggacac cacttcctga taccggcgac ggctcttgac
     6061 ggtgcgctcc acctcaaacg ccaccgtttt accgtccagc tccgccaggg cgtccggcac
     6121 tttcatgcct ttaagactca gacggtgcag aatgcgccag ccggtggccc ctctggcttc
     6181 catatcaagg cgcagggatt gcacggccag ccggtgcggc agatgtgctg ccgacaccct
     6241 tgacggctga aacggaataa catccgccag cggttcgtcc tcgtcgaacg agaacgccac
     6301 gccatgcggc gtcagccccc acacgctgac ctgacggccg tccaccggat atttggtccg
     6361 gaccagcagc cctttcgcca ccaggctgtc gaggaagcgg tagaccggct gcaccgccgc
     6421 aatccccagc cgccgcgcaa ccagctccgc cgtggaacac agctcttccc gcaaccagcg
     6481 cagggtcagc cgggttttct ccgcgttccg cgcctgtcgt ttcgcgtggc tgacgttgta
     6541 cagctccagc gccatcagta aatccccgca aaatcatcat ccgctttcgg cccgccgccg
     6601 tcggccgggg gccggaactc cgccagggga tcggttttcg ccagcaccgg gaaacgctgc
     6661 aacgggattt cccggcgctc aacccgcagc ggactgctga acgccatccg ggccacaccc
     6721 agcccggtca tgaccgcgaa cccgtccggc aggtgttgca gggtgttcac gtcaaacagc
     6781 ggccgcgcct ccttctggat gaagctgtcc ccgctgatat gctccacgtt ccccgcctcc
     6841 cggctggcac ggcggcgctc cacgtccacc ctgatttccc cggtctgccc cgccgcccac
     6901 tgcgcggact ccaggctggc cgcccggtaa aaccagcgga tcggcgtgtt atccagtacc
     6961 gtggttttca caaattcagc gggcagatcc tgcccgcact gcccgaaatc gcccagcgac
     7021 tggtgcgcga gcaacagatt gcagtcccgg ctgcgcagcg tccccagtgc gttcagtaca
     7081 tacttcgaca gcagatattt aatttcatcg agcatcacgc tgacgtgtgg ccactcccgg
     7141 aactcgtccc gcgcaatgat gatttgcgcg cagcgggcaa acagcatttt ttgcacacgg
     7201 atcaccgcct cgtcgtccat cgagccgata acgtacaggc agccgccgcc gttgataatc
     7261 cccgccacat cgatgccact gtccgtctgc aggacgctca ggtcagcgac gttttccagc
     7321 ctagtgatca accccttcac gtcctttttc agggcctccg gcagcgcgta cgccgccgcc
     7381 agcacctgct gaatgtttgc cccctgcgga aactgctccg caatcagctt cgccgctttc
     7441 tgctcgctga tgcggtagtg gtccgccacg tcgccggtct cgcccaggtt aagcccggca
     7501 atcagcaggt ttttcagcgc gtactggtcg agatcgcgga acagattcag ctgcgccggt
     7561 ttccccggcc gcagatccag cagggtgaag tccggacagt gcgcccggaa gacgtgcggc
     7621 gcccatgcgt cccctttggg gtcgaagtac accaccgcgt ccccgaaatc gcgtacgcag
     7681 cgaatgagcg cgttggtggc catcacgctc ttgccgctcc cgggcaggcc gaggatctga
     7741 atgttggtct tgcgccaggt atcacgcgga accagcaccg gcttgccctg ctcatcctta
     7801 cccagaaaca ccgcctgctc ctgccgggcc tgctccgagg aaaaatcggc cagcgcatta
     7861 accggtaccg gctccccgaa atgcaggtgg cgcacgtcgg tcatctctcc tgtccgctgg
     7921 ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg cctcgtcagc agccgcaaaa
     7981 tgaccgtcaa gcagcaggaa cgccagcagc cgcgccaaca caatcccgac agccagcccg
     8041 cccgcgcacc aggcaatccc gaacaggtta gccggctgca gcaggtaata atctgcgttt
     8101 gcgtagaggt ccggacgcag gacggtttct ccccagctct cgataaacac ggcgacaacc
     8161 agccccccca gaagggccag accggccgcg ccggtcagcc agtacgcgta gttccagaca
     8221 tgtcgacgtg ccctgaccat ccgcccgggc gaatccagcg aggccagcac cgaaaatggc
     8281 gctggccgtt tattatcgcg cttatagcga tcggcatact cccgtcgctc cttatgactc
     8341 agccccacaa aacgcatccg tccggcccaa agttcagcca gaccgaaaaa caggccgata
     8401 actaggaaag ccccggccag accgtaatac tcctggtaca gccagctgac ccacggataa
     8461 atctgccggg ccagctccgg aatgtgccac ttccaggcaa gcgaatccgt attaaacatg
     8521 ttctctctcc ttcagacgat ggtttgccgg cgtgatgtgc agcccccgct gcacaagccg
     8581 cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg ttcagctttc
     8641 accacggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg gaggaacaac
     8701 gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt ttccggtgtt
     8761 ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat caggagcagc
     8821 tttgctgctt cgccaggtgc gtccagcacc gtggattttt gtgggtggtc aggcgtgagc
     8881 ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc agccggacgg agccagaaag
     8941 agggggagag accgtgcgtc ggtggcgtcc agccagcgta agcaggggat atccccctga
     9001 ggtcagccac gccctcgcgg ggatgacctg tatttctccg taaggagaaa aaagcaacag
     9061 agataggtta acgtaaaatg ttcagcgaaa aaccgatatg attcacctat cctggtaatt
     9121 tattatcagg gaatataaag acttaatcag gggacaatat gccacatcag cgcgtccggt
     9181 gtccgtcttg taggtgccgg ggaaattgcg cctcctcgct cactcggtcg ctacgctccg
     9241 gccgtgagac tgcggcgggc gctgcggaca catgcaaagt tacccacaga ttcc
//
