LOCUS       NZ_CP149998             9294 bp    DNA     circular CON 15-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2023CK-01686 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP149998
VERSION     NZ_CP149998.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39334901
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 9294)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Flye v. 2.9.3
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 50.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038098145.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 12:46:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP149998.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023CK-01686"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2023-12-08"
     gene            32..211
                     /locus_tag="WLR01_RS00005"
                     /old_locus_tag="WLR01_00005"
     CDS             32..211
                     /locus_tag="WLR01_RS00005"
                     /old_locus_tag="WLR01_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(239..769)
                     /locus_tag="WLR01_RS00010"
                     /old_locus_tag="WLR01_00010"
     CDS             complement(239..769)
                     /locus_tag="WLR01_RS00010"
                     /old_locus_tag="WLR01_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(776..1507)
                     /locus_tag="WLR01_RS00015"
                     /old_locus_tag="WLR01_00015"
     CDS             complement(776..1507)
                     /locus_tag="WLR01_RS00015"
                     /old_locus_tag="WLR01_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(1507..3471)
                     /locus_tag="WLR01_RS00020"
                     /old_locus_tag="WLR01_00020"
     CDS             complement(1507..3471)
                     /locus_tag="WLR01_RS00020"
                     /old_locus_tag="WLR01_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4952..5101)
                     /locus_tag="WLR01_RS00025"
                     /old_locus_tag="WLR01_00025"
     CDS             complement(4952..5101)
                     /locus_tag="WLR01_RS00025"
                     /old_locus_tag="WLR01_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5186..5443)
                     /locus_tag="WLR01_RS00030"
                     /old_locus_tag="WLR01_00030"
     CDS             complement(5186..5443)
                     /locus_tag="WLR01_RS00030"
                     /old_locus_tag="WLR01_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(5453..7138)
                     /locus_tag="WLR01_RS00035"
                     /old_locus_tag="WLR01_00035"
     CDS             complement(5453..7138)
                     /locus_tag="WLR01_RS00035"
                     /old_locus_tag="WLR01_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7541..7960
                     /locus_tag="WLR01_RS00040"
                     /old_locus_tag="WLR01_00040"
     CDS             7541..7960
                     /locus_tag="WLR01_RS00040"
                     /old_locus_tag="WLR01_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8266..8529
                     /locus_tag="WLR01_RS00045"
                     /old_locus_tag="WLR01_00045"
     CDS             8266..8529
                     /locus_tag="WLR01_RS00045"
                     /old_locus_tag="WLR01_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8519..8818
                     /locus_tag="WLR01_RS00050"
                     /old_locus_tag="WLR01_00050"
     CDS             8519..8818
                     /locus_tag="WLR01_RS00050"
                     /old_locus_tag="WLR01_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8880..9257)
                     /locus_tag="WLR01_RS00055"
                     /old_locus_tag="WLR01_00055"
     CDS             complement(8880..9257)
                     /locus_tag="WLR01_RS00055"
                     /old_locus_tag="WLR01_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     BGC             453..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4271..4849
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(780..1507)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 aggcgcacac catgcccgga aggaaagaat catgacaaaa caggaaaaaa cagccctcaa
       61 tatggctaaa tttattaagg agcagtcgct gctattgctc gataaactga atgagctgga
      121 tatggatacc ccggccgaca tgtgcgagcg gctacacgag gatgcggaaa cactctggca
      181 ggtgatgcgg gataagctcg gggaggaata acagcaccgg catggccggt gctggcattt
      241 attttttgcc gaacggccac caccggcggg caggtttgtc ctccggcagt tcaggtaccg
      301 gcacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc ttctcgttca
      361 ccgcgctttc cccgtgccgc tcactcacca cgtcaatcat cgcgttaatc tgctccggtg
      421 agcacttccc ggcatcaacc acctgcacac cgttctcgat ggcgaaccgc tccaccttgc
      481 gatagaggta atcacacagc ttcattcctg tgggtttgtt ccgttcccat tgcggatttt
      541 taccagtgaa aaagtacgtg gctttttcgt cgctgttgag ccgctcgtca tacatcccga
      601 tggatttcat catcgccaga tgctgggccc ggtcgacaaa acagaaaaca tacgccccgg
      661 tctgccaggc gagcaccacc atatcttcca gatgctcgct ggtcggcatg atgcagcgct
      721 cgccgttccg gtcctccgcg tcttcaataa ataccatgta cagcgccata atccctcact
      781 ccgttgtcat aaatttaaag cacgcaaaca accgttcacg atccagctct gccgttttgc
      841 gggcctcgcc ggtctgtggg ttcacgattt catccaccga caggattgcc cgctgcaccc
      901 tgacctgcgt cgcccggtcc gggcagatgt accagatttc atcgatgccg ttagctttgc
      961 ggttgaacag atacccggac accacttcct gataccggcg acggctcttg acggtgcgct
     1021 ccacctcaaa cgccaccgtt ttaccgtcca gctccgccag ggcgtccggc actttcatgc
     1081 ctttaagact cagacggtgc agaatgcgcc agccggtggc ccctctggct tccatatcaa
     1141 ggcgcaggga ttgcacggcc agccggtgcg gcagatgtgc tgccgacacc cttgacggct
     1201 gaaacggaat aacatccgcc agcggttcgt cctcgtcgaa cgagaacgcc acgccatgcg
     1261 gcgtcagccc ccacacgctg acctgacggc cgtccaccgg atatttggtc cggaccagca
     1321 gccctttcgc caccaggctg tcgaggaagc ggtagaccgg ctgcaccgcc gcaatcccca
     1381 gccgccgcgc aaccagctcc gccgtggaac acagctcttc ccgcaaccag cgcagggtca
     1441 gccgggtttt ctccgcgttc cgcgcctgtc gtttcgcgtg gctgacgttg tacagctcca
     1501 gcgccatcag taaatccccg caaaatcatc atccgctttc ggcccgccgc cgtcggccgg
     1561 gggccggaac tccgccaggg gatcggtttt cgccagcacc gggaaacgct gcaacgggat
     1621 ttcccggcgc tcaacccgca gcggactgct gaacgccatc cgggccacac ccagcccggt
     1681 catgaccgcg aacccgtccg gcaggtgttg cagggtgttc acgtcaaaca gcggccgcgc
     1741 ctccttctgg atgaagctgt ccccgctgat atgctccacg ttccccgcct cccggctggc
     1801 acggcggcgc tccacgtcca ccctgatttc cccggtctgc cccgccgccc actgcgcgga
     1861 ctccaggctg gccgcccggt aaaaccagcg gatcggcgtg ttatccagta ccgtggtttt
     1921 cacaaattca gcgggcagat cctgcccgca ctgcccgaaa tcgcccagcg actggtgcgc
     1981 gagcaacaga ttgcagtccc ggctgcgcag cgtccccagt gcgttcagta catacttcga
     2041 cagcagatat ttaatttcat cgagcatcac gctgacgtgt ggccactccc ggaactcgtc
     2101 ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatt ttttgcacac ggatcaccgc
     2161 ctcgtcgtcc atcgagccga taacgtacag gcagccgccg ccgttgataa tccccgccac
     2221 atcgatgcca ctgtccgtct gcaggacgct caggtcagcg acgttttcca gcctagtgat
     2281 caaccccttc acgtcctttt tcagggcctc cggcagcgcg tacgccgccg ccagcacctg
     2341 ctgaatgttt gccccctgcg gaaactgctc cgcaatcagc ttcgccgctt tctgctcgct
     2401 gatgcggtag tggtccgcca cgtcgccggt ctcgcccagg ttaagcccgg caatcagcag
     2461 gtttttcagc gcgtactggt cgagatcgcg gaacagattc agctgcgccg gtttccccgg
     2521 ccgcagatcc agcagggtga agtccggaca gtgcgcccgg aagacgtgcg gcgcccatgc
     2581 gtcccctttg gggtcgaagt acaccaccgc gtccccgaaa tcgcgtacgc agcgaatgag
     2641 cgcgttggtg gccatcacgc tcttgccgct cccgggcagg ccgaggatct gaatgttggt
     2701 cttgcgccag gtatcacgcg gaaccagcac cggcttgccc tgctcatcct tacccagaaa
     2761 caccgcctgc tcctgccggg cctgctccga ggaaaaatcg gccagcgcat taaccggtac
     2821 cggctccccg aaatgcaggt ggcgcacgtc ggtcatctct cctgtccgct ggctgctgcg
     2881 ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca gcagccgcaa aatgaccgtc
     2941 aagcagcagg aacgccagca gccgcgccaa cacaatcccg acagccagcc cgcccgcgca
     3001 ccaggcaatc ccgaacaggt tagccggctg cagcaggtaa taatctgcgt ttgcgtagag
     3061 gtccggacgc aggacggttt ctccccagct ctcgataaac acggcgacaa ccagcccccc
     3121 cagaagggcc agaccggccg cgccggtcag ccagtacgcg tagttccaga catgtcgacg
     3181 tgccctgacc atccgcccgg gcgaatccag cgaggccagc accgaaaatg gcgctggccg
     3241 tttattatcg cgcttatagc gatcggcata ctcccgtcgc tccttatgac tcagccccac
     3301 aaaacgcatc cgtccggccc aaagttcagc cagaccgaaa aacaggccga taactaggaa
     3361 agccccggcc agaccgtaat actcctggta cagccagctg acccacggat aaatctgccg
     3421 ggccagctcc ggaatgtgcc acttccaggc aagcgaatcc gtattaaaca tgttctctct
     3481 ccttcagacg atggtttgcc ggcgtgatgt gcagcccccg ctgcacaagc cgcggacatg
     3541 ccccaaggca tggcggcggc ggtcacgtcc gtggtgattg cgttcagctt tcaccacgga
     3601 ggccagcctc tttccgcccc atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc
     3661 cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc
     3721 cgtcagagtt cgccatagcg agcggctgac cacccgcaaa atcaggagca gctttgctgc
     3781 ttcgccaggt gcgtccagca ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg
     3841 ggccgggtga atttctggcg tggccggcgt ccagccggac ggagccagaa agagggggag
     3901 agaccgtgcg tcggtggcgt ccagccagcg taagcagggg atatccccct gaggtcagcc
     3961 acgccctcgc ggggatgacc tgtatttctc cgtaaggaga aaaaagcaac agagataggt
     4021 taacgtaaaa tgttcagcga aaaaccgata tgattcacct atcctggtaa tttattatca
     4081 gggaatataa agacttaatc aggggacaat atgccacatc agcgcgtccg gtgtccgtct
     4141 tgtaggtgcc ggggaaattg cgcctcctcg ctcactcggt cgctacgctc cggccgtgag
     4201 actgcggcgg gcgctgcgga cacatgcaaa gttacccaca gattccgtga ataagcaggg
     4261 gactaacatg tgaggcaaaa cggcaaggcc acgcaggtgg cgtttttcca taggctccgc
     4321 cctcctgact gagttcacat aaacagacgc tttttcggtg catctgtggg agccggtggc
     4381 tcaaccatga atcagtcagg agtggcgaaa cccgacagga cttaaagatc atcaccgttt
     4441 ccgatgaatc gctccctctt gcgctctcct gttccaaccc tgccgtttcc cggatacctg
     4501 ttccgccttt ctcccttgcg ggaagtgtgg cgctttctca tagctcacac tctggtatct
     4561 cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaacccc ccgttcagcc
     4621 cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccggaaa agcacgcaaa
     4681 acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca
     4741 cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg
     4801 ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg
     4861 atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa
     4921 aaaggatctc aagaagatcc tttgatcttt tctactgaac cgcgatcccc gtcagttcag
     4981 aggaggacga tggtgccacc gttccaccct gaacatcccg gatatagttt gcctgacatg
     5041 cgaccagaag aaagcctgaa aaaatcaata taaaaagaaa aacaaccttt acctttttca
     5101 tataccttcc ctgtatttaa aaaccccggt attcatccct gaacaccgga agtctgtatt
     5161 tcaaatcatc cttgataact tttgcttacc agttaccatc ccggtaaaca aaggatacaa
     5221 agtaatcaaa tttactaata tcgattacat ttttaaagtg tggctgtaat atcgaaaccc
     5281 atgatttttc aacatcaaac caaccattat taatattatc ctttaaaggc atccccagac
     5341 tttcaatgac agaaccatca tcaccgaagt cttttgagta ttcaccacct ttaaactctt
     5401 cggttttctt atcaaaccaa tgaatatgta atttaagccc catatttacc tcttaaagat
     5461 attttttaat gtttcgtttt ggatccggcc ctttaacctg cttacccgtt tttggatcga
     5521 atgccccgag gtgttcgcca tcactggcgc ggtacccttc aagctcaccg tgctgggaat
     5581 cccattcata aattttacgc tttttatcac cataccatcg ggcccgctta ccaccaccac
     5641 cttgtttagg ggttttaggt ttaccctctt tcaactctcc caacccctta atatcttcag
     5701 tcttgggatc aggaaaataa tcatggccgt aatctttagt tcccttacga ggttttttct
     5761 tttcctcatt taatttattt tctgcatcct tcgctttttt ctccttttgt ttccggctct
     5821 ccatcgcccc ggaaagcgca gccttatccc tggataagga ttcattcatt tcgtttaaag
     5881 atgtctgggc agcttttaat ttctcattct gattctgaat atccttatca agatagcgaa
     5941 cctgatcctg ccacccggag ccatattcat gaggcgggtt ataagtcaca aaatcatctt
     6001 tatttgcctc tgcctctttt acctttttat cggcttcctg aacagcagcc cttgcaccgg
     6061 ggatcgcctt ctctgtcgat gcaatgcgac tgtttaaggt cgcaatattt ttatcttcag
     6121 catccagctc agctttcgct ttatcatact ctcttttcag cccctcttcc gggtgagcag
     6181 cgtcccatgc ctgctggcga cgcttttctt cctccagacg ctgttttacc tgtgccggag
     6241 taagaacatc cgtcaccgac acatagaccg gtttctgtcc gctctctttc gggaaacgaa
     6301 taacggcttc acgggagtta ccaccggccg taaaaccagc cggacgtgaa tcaccttttt
     6361 cagcaataat gccttttggc ggggctttcg cggccggaac acctttaggt acgctcacct
     6421 gcagagacgg gagacccgga atcgacacac tgaatacccc cggacgtttt gtcggtttcg
     6481 catccaccac aggaacactc attggccggc cggtgacaac cgcaatatgc tgccgctcgt
     6541 ccttcacaat atccgccaca cgtttagtga cgctgaccgt cgcctggtcc agcggcaggg
     6601 agctgaccgg cgtctccgtt accgtgtctg ccggcaatga cgtcacgatt tttgacatca
     6661 ttttcggatc atcttttgct atctcagaag gcagcactcc gtaaatcgca atcccccaca
     6721 gaccaaactt aaacggacct ttcagggcag ccagcacatc agcaacagcg gccgacaacg
     6781 catcgccgga aacggataaa gccagtcctt cagcccccgg agtggccaag gcaggcagac
     6841 cgaaggccat cgcggtggcc gaagactgtc cgccaccaga gctcccgcta tgattgccgc
     6901 tgttaccgcc attaccaccg ggtttactcc cgccgttacc aaactccccc gggttatagt
     6961 tatgaatatc cccgttaggt gtatgcgtcg taccccagcc cggcccgctg tttggatccg
     7021 taccaccacc tgatgaaggg ccacctttac ctgatgtacc gttcacattc ccactggcct
     7081 gaccaccatt gtgtcccaga cctgaattac ccggacctcg tccgtctccg ccactcatat
     7141 acttttcctc tttgactttt aaaacaataa actaaaataa aatactgtac atataaccac
     7201 tggttttata tacagtataa aaattacgct gccttgtttt tcatgtcaag tctgtaaatt
     7261 tgcgtttttg tgaaaatgat caaaaaaaca gccactcaca ccatgcgcaa tcccctccag
     7321 atgcaacaaa aaccagcaca aacggcgccg ttctccggcc tcagtatgtt ttactgaaat
     7381 cattatatga gataccagaa agccccctcc gggacaggaa aagaaacggg gggtctggtt
     7441 cgggttgttc ggtcactgaa cacggtcaga aactgatgat aagatagcct gcggatatat
     7501 aactcaatga gaaaaaagaa ttaaaggtaa ggagacgctc atgagacgtt ccggcagcaa
     7561 actgtgtctt atcgccatga taacagcgac agcaactctc actgcctgtg ctccaaaggg
     7621 cagcgtggaa cagcataccc ggcattacgt atatgcttca gatgacggtt ttgatcccaa
     7681 tttttccacc cagaaagctg acacaacacg aatgatggtg ccttttttcc ggcagttctg
     7741 ggatatgggc gcaaaggata aagcgaccgg aaaatcacgg agtgatgtgc agcaacgcat
     7801 tcagcagttt cacagtcagg aatttttaaa ctcactccaa ggcacaacac aatttgcggg
     7861 taccgattac cgcagcaaag acctcacacc gaaaaaatca aggctactgg ctgacaccat
     7921 ttcagctgtt tatctcgatg gctatgaagg cagacagtaa gtggatttac cataacgcgt
     7981 cattacgcgc accgctcaga cgggctcagg gcgtttccgg cggctacggg ataaaacagg
     8041 caacaacccc cccggaaaac tgccggaaac gcgcccgata aattttatcc taatgcatgc
     8101 ctatgcacgc aactgcatgg catatgtttt gacgtaaaat ggatgccgta agcccttttt
     8161 taccgacttt ctgcttcatc agcctgactg acacgaccat attctttcag accgccccct
     8221 gctaaacttc cttcatattg ccgcattaac ccgggaggct ccgcaatgag caacactaca
     8281 cactacgaaa acgccaactt tcttcgggaa ctggctgaaa gcctgccccg catactgccg
     8341 gaaggtggcc cggacaaagc tgcgctgttg cagcgactgg ctaacgaaga actggcccag
     8401 gcagagtatg aagatcaggt tcgcgccaaa gtgaccgccg cccgtgctga cacgcgtccg
     8461 ggtatgacca cggaacaact ccgccagcgc ctgcatggcc gttatcagga actgcgcgat
     8521 gccgtatgac gtggaatgga agcagggagc catcgaggat gtaacgacgc tgtttgacta
     8581 tatcgcggaa aattcatccc tgtgggatgc gcagaacgtt accgaccggg ttctcgctgc
     8641 ggcggacaaa ctggccgact tcccgcgact ctatgaagtg gactcacgct atggcgagga
     8701 cgttcgccgg atcagtctga tgggacagca tgtcctctat gatgttgatg accagaccag
     8761 aaaagtccgg gttctggccg tggtcggcca gcgccagaat ccccatattg ttctctaata
     8821 accggtgacg gggacgcaat tcattgcgtg gccgtcgccg gttggacaag cacagcgcgt
     8881 cagcgttttc cccacggcca ccaccggcgc ggtgttccgg ccggggtctc tgctgtctcc
     8941 ggtttgtgtt ccagcagtct catggactgc atttcctgcc gtagcccggc cacctcctcc
     9001 ctgagtgctt ttatctccgc gatcaacgcg tcggaggcca ttttttcagt ctcacccgac
     9061 agtctcaact cgtcccgctc gtgagtctca tcctgtctta actcaccgta cacccggata
     9121 agctcgctgg tatccaccac ccggccgcca tcagcttccg tacggtatga tacgcggcct
     9181 ttcgccatat cccgatacag ggaacttcgg gctttacccg tcagttccgt cgcctgtctc
     9241 accgtcaccc acgccattgt ctcaccccgc tatttttgag acagtatacc tgac
//
