LOCUS       NZ_CP149844             2622 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain 2024CK-00066 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP149844
VERSION     NZ_CP149844.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39595401
            Assembly: GCF_036080935.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 2622)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036080935.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 15:31:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,664
            CDSs (total)                      :: 4,548
            Genes (coding)                    :: 4,463
            CDSs (with protein)               :: 4,463
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 20 of 85
            Pseudo Genes (incomplete)         :: 62 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 14 of 85
            Pseudo Genes (short protein)      :: 1 of 85
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP149844.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2024CK-00066"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2023-12-26"
     gene            join(2593..2622,1..402)
                     /locus_tag="WKU57_RS23310"
                     /old_locus_tag="WKU57_23310"
     CDS             join(2593..2622,1..402)
                     /locus_tag="WKU57_RS23310"
                     /old_locus_tag="WKU57_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660103.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_069970468.1"
                     /translation="MKAKTEPLEKKWGERSMLMGWTAIPTSLLFLQAELSISPLGFNLL
                     MNLVSHWWETTEHPYPSQESMAKRMGVSKRSIQREITTLVELGLLDKQPNKAGSKKFKG
                     RNTYDLSKLVQVLNEKTPDKISSFKRYKKNGESLDSELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            412..864
                     /locus_tag="WKU57_RS23315"
                     /old_locus_tag="WKU57_23315"
     CDS             412..864
                     /locus_tag="WKU57_RS23315"
                     /old_locus_tag="WKU57_23315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142353804.1"
                     /translation="MPTFTQSGTGKFDYWLIDGIKSFSKIPANTLPSITVDMPIRLQVG
                     NGYFGSTHITGRHGKWLQRYQPDGCVATFIHKKLSTSGKILLLEEQGKIGLALRLNPDS
                     ALILKNIGDFFSVTTIYYKRSGLQGEEIGRYTGSSWATSPFIDRKR"
                     /name="hypothetical protein"
     gene            1909..2079
                     /locus_tag="WKU57_RS23320"
                     /old_locus_tag="WKU57_23320"
     CDS             1909..2079
                     /locus_tag="WKU57_RS23320"
                     /old_locus_tag="WKU57_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077269784.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHEKTPLAAAIGKKVYKKITS
                     AEFLKSEPNYG"
                     /name="replication initiation protein"
     Typing          1730..2200
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.712"
                     /coverage="91.0"
                     /evalue="2.78e-170"
ORIGIN
        1 aagtggggag aacgaagcat gcttatgggc tggacagcga tccctacatc actgcttttt
       61 ctacaggcgg aactatcaat tagtcctttg ggtttcaatt tgctgatgaa ccttgtttct
      121 cactggtggg aaactacaga gcacccttac ccttcgcaag aatcgatggc aaaaagaatg
      181 ggcgtttcga aacgttctat ccagagagaa atcacaactt tggtagagct tggcttactc
      241 gataaacagc cgaataaggc cggaagcaag aaatttaaag gtcgaaacac ctatgactta
      301 agcaagcttg ttcaagtcct taatgaaaaa acacccgata aaataagcag tttcaaacgt
      361 tataaaaaaa atggagaatc actagattca gagctggagt aaccttagaa aatgccgaca
      421 ttcacgcaat ctggaactgg gaaatttgat tattggctta tagatggaat taagtctttt
      481 tcaaaaatac cggctaacac actgccatca atcaccgtag acatgccgat ccgcttacaa
      541 gtcggaaatg gttactttgg ctcaacacac ataaccggca ggcatggtaa gtggcttcaa
      601 cgctatcaac cagatggttg tgtagcaaca ttcatccata agaagctcag caccagtggg
      661 aagatcttac tgttggaaga gcaaggtaaa ataggtttag ctctcagact aaacccagac
      721 tcagctctga tactgaaaaa cattggcgat tttttctctg taacaacaat ctattacaaa
      781 cgaagtgggc ttcagggaga ggaaatagga aggtataccg gttcatcgtg ggcgacatct
      841 ccctttatcg atagaaagcg ataaccccac caagatttac cataacatct cttgggcgca
      901 ccgcctgaga gcctctcagc gcgtttctgc ggccccctgc cgattttcgg cacctttccc
      961 cgcccatacc cctgaaaaac gcccctgatg atttctgacc ggctgcatgg ctatgcacag
     1021 aagtgaatgg ctggcagttt cacgcgtatg ttgtcactaa ggccgctttt tcgcaggaaa
     1081 cggtcatttc ctataacggt gaacgggcgc gcagtttact gtgcgatcgt gaggcgttga
     1141 gcaacgagct ttgcgaggcg gtaggggttc agagagccag ctgcaggctc aggagaaaac
     1201 attctgtttt cggggcaaac gccgcaaatg acgtcatttt gcggcgtttg ccctatcctg
     1261 catcgcagca tacggtgcca tccgccaccc tcttgcgaat cctgccctag atctcaacag
     1321 cagagaaatt tcgtacgtac gaaattcagc gtatcgggcg gcgcctgatg aaacattctg
     1381 gagtgtcggc ggtactccaa aaacacatct tgtcccgctg attttgtcac tggcgcaaag
     1441 cggcggtaat gacaagacat ttcctgtccg catcaatacc aatacgtgca gaaaacgtaa
     1501 gtggtgaaag atgatagtac ggagcgacag gagcctaagc agccaccgga gaaattacgc
     1561 ttcctcggtc attcgctcac tgcgttcggt catgactctg cggcgagcgt tacggggtca
     1621 atcaaagggc gtggaggtat gaagtgtgag aagagactta agggctgccg gttttccata
     1681 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagcgg tggcgaaacc
     1741 cgacaggact ataaagatcc caggcgtttc cccctggtag ctccctcgtg cgctctcctg
     1801 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca
     1861 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1921 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1981 agacacgaaa aaacgccact ggcagcagcc attggtaaga aggtgtacaa gaagattact
     2041 agtgcagagt tcttgaagtc tgagccaaac tacggctaaa ctggaaggac gaatttggtg
     2101 gcagcggtct tgtacaccct gttaccacgg ttcataaact tgccggttta atgaaccttc
     2161 gaaaaaccac cttgccgggt ggttttttcg tttccgagac tacatttttg cttaccttcc
     2221 tgtcaatcaa gaagataaca aacaaaaaaa gctgcctagg gcagcttttt ttatgagtcc
     2281 gagtggcgta cgtgatgtct gttatcagat tcatcatccc tctctgtgag ggagcccact
     2341 caagtggctg cgaaatgcag ggcgatccac tatcagcgca tgttgcctcg ccattatatc
     2401 gccatgacgg acaaatcgtc ttctgtaaga ctcatagcaa gtatggtgac aaaaccatat
     2461 caaataaagg cgacaaaatg tctatacata tgaatataca tatataatta taaccaagaa
     2521 cactctaaca tcattaaata atgatgttag agtgttcttg gtgtcgctac aaattaaaaa
     2581 acgaggttcg taatgaaagc gaagacagag ccacttgaaa aa
//
