LOCUS       NZ_CP149584             9294 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 2024CK-00065 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP149584
VERSION     NZ_CP149584.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39595402
            Assembly: GCF_037890715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2024) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037890715.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 22:07:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,563
            CDSs (total)                      :: 5,442
            Genes (coding)                    :: 5,310
            CDSs (with protein)               :: 5,310
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 48 of 132
            Pseudo Genes (incomplete)         :: 80 of 132
            Pseudo Genes (internal stop)      :: 24 of 132
            Pseudo Genes (multiple problems)  :: 17 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP149584.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2024CK-00065"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2023-12-14"
     gene            complement(join(8960..9294,1..1351))
                     /locus_tag="WJ445_RS27765"
                     /old_locus_tag="WJ445_27765"
     CDS             complement(join(8960..9294,1..1351))
                     /locus_tag="WJ445_RS27765"
                     /old_locus_tag="WJ445_27765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1754..2173
                     /locus_tag="WJ445_RS27770"
                     /old_locus_tag="WJ445_27770"
     CDS             1754..2173
                     /locus_tag="WJ445_RS27770"
                     /old_locus_tag="WJ445_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2479..2742
                     /locus_tag="WJ445_RS27775"
                     /old_locus_tag="WJ445_27775"
     CDS             2479..2742
                     /locus_tag="WJ445_RS27775"
                     /old_locus_tag="WJ445_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2732..3031
                     /locus_tag="WJ445_RS27780"
                     /old_locus_tag="WJ445_27780"
     CDS             2732..3031
                     /locus_tag="WJ445_RS27780"
                     /old_locus_tag="WJ445_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3093..3470)
                     /locus_tag="WJ445_RS27785"
                     /old_locus_tag="WJ445_27785"
     CDS             complement(3093..3470)
                     /locus_tag="WJ445_RS27785"
                     /old_locus_tag="WJ445_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            3539..3718
                     /locus_tag="WJ445_RS27790"
                     /old_locus_tag="WJ445_27790"
     CDS             3539..3718
                     /locus_tag="WJ445_RS27790"
                     /old_locus_tag="WJ445_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3746..4276)
                     /locus_tag="WJ445_RS27795"
                     /old_locus_tag="WJ445_27795"
     CDS             complement(3746..4276)
                     /locus_tag="WJ445_RS27795"
                     /old_locus_tag="WJ445_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4283..5014)
                     /locus_tag="WJ445_RS27800"
                     /old_locus_tag="WJ445_27800"
     CDS             complement(4283..5014)
                     /locus_tag="WJ445_RS27800"
                     /old_locus_tag="WJ445_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(5014..6978)
                     /locus_tag="WJ445_RS27805"
                     /old_locus_tag="WJ445_27805"
     CDS             complement(5014..6978)
                     /locus_tag="WJ445_RS27805"
                     /old_locus_tag="WJ445_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8459..8608)
                     /locus_tag="WJ445_RS27810"
                     /old_locus_tag="WJ445_27810"
     CDS             complement(8459..8608)
                     /locus_tag="WJ445_RS27810"
                     /old_locus_tag="WJ445_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8693..8950)
                     /locus_tag="WJ445_RS27815"
                     /old_locus_tag="WJ445_27815"
     CDS             complement(8693..8950)
                     /locus_tag="WJ445_RS27815"
                     /old_locus_tag="WJ445_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     BGC             1..6351
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7778..8356
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4287..5014)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggaaagc gcagccttat
       61 ccctggataa ggattcattc atttcgttta aagatgtctg ggcagctttt aatttctcat
      121 tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg gagccatatt
      181 catgaggcgg gttataagtc acaaaatcat ctttatttgc ctctgcctct tttacctttt
      241 tatcggcttc ctgaacagca gcccttgcac cggggatcgc cttctctgtc gatgcaatgc
      301 gactgtttaa ggtcgcaata tttttatctt cagcatccag ctcagctttc gctttatcat
      361 actctctttt cagcccctct tccgggtgag cagcgtccca tgcctgctgg cgacgctttt
      421 cttcctccag acgctgtttt acctgtgccg gagtaagaac atccgtcacc gacacataga
      481 ccggtttctg tccgctctct ttcgggaaac gaataacggc ttcacgggag ttaccaccgg
      541 ccgtaaaacc agccggacgt gaatcacctt tttcagcaat aatgcctttt ggcggggctt
      601 tcgcggccgg aacaccttta ggtacgctca cctgcagaga cgggagaccc ggaatcgaca
      661 cactgaatac ccccggacgt tttgtcggtt tcgcatccac cacaggaaca ctcattggcc
      721 ggccggtgac aaccgcaata tgctgccgct cgtccttcac aatatccgcc acacgtttag
      781 tgacgctgac cgtcgcctgg tccagcggca gggagctgac cggcgtctcc gttaccgtgt
      841 ctgccggcaa tgacgtcacg atttttgaca tcattttcgg atcatctttt gctatctcag
      901 aaggcagcac tccgtaaatc gcaatccccc acagaccaaa cttaaacgga cctttcaggg
      961 cagccagcac atcagcaaca gcggccgaca acgcatcgcc ggaaacggat aaagccagtc
     1021 cttcagcccc cggagtggcc aaggcaggca gaccgaaggc catcgcggtg gccgaagact
     1081 gtccgccacc agagctcccg ctatgattgc cgctgttacc gccattacca ccgggtttac
     1141 tcccgccgtt accaaactcc cccgggttat agttatgaat atccccgtta ggtgtatgcg
     1201 tcgtacccca gcccggcccg ctgtttggat ccgtaccacc acctgatgaa gggccacctt
     1261 tacctgatgt accgttcaca ttcccactgg cctgaccacc attgtgtccc agacctgaat
     1321 tacccggacc tcgtccgtct ccgccactca tatacttttc ctctttgact tttaaaacaa
     1381 taaactaaaa taaaatactg tacatataac cactggtttt atatacagta taaaaattac
     1441 gctgccttgt ttttcatgtc aagtctgtaa atttgcgttt ttgtgaaaat gatcaaaaaa
     1501 acagccactc acaccatgcg caatcccctc cagatgcaac aaaaaccagc acaaacggcg
     1561 ccgttctccg gcctcagtat gttttactga aatcattata tgagatacca gaaagccccc
     1621 tccgggacag gaaaagaaac ggggggtctg gttcgggttg ttcggtcact gaacacggtc
     1681 agaaactgat gataagatag cctgcggata tataactcaa tgagaaaaaa gaattaaagg
     1741 taaggagacg ctcatgagac gttccggcag caaactgtgt cttatcgcca tgataacagc
     1801 gacagcaact ctcactgcct gtgctccaaa gggcagcgtg gaacagcata cccggcatta
     1861 cgtatatgct tcagatgacg gttttgatcc caatttttcc acccagaaag ctgacacaac
     1921 acgaatgatg gtgccttttt tccggcagtt ctgggatatg ggcgcaaagg ataaagcgac
     1981 cggaaaatca cggagtgatg tgcagcaacg cattcagcag tttcacagtc aggaattttt
     2041 aaactcactc caaggcacaa cacaatttgc gggtaccgat taccgcagca aagacctcac
     2101 accgaaaaaa tcaaggctac tggctgacac catttcagct gtttatctcg atggctatga
     2161 aggcagacag taagtggatt taccataacg cgtcattacg cgcaccgctc agacgggctc
     2221 agggcgtttc cggcggctac gggataaaac aggcaacaac ccccccggaa aactgccgga
     2281 aacgcgcccg ataaatttta tcctaatgca tgcctatgca cgcaactgca tggcatatgt
     2341 tttgacgtaa aatggatgcc gtaagccctt ttttaccgac tttctgcttc atcagcctga
     2401 ctgacacgac catattcttt cagaccgccc cctgctaaac ttccttcata ttgccgcatt
     2461 aacccgggag gctccgcaat gagcaacact acacactacg aaaacgccaa ctttcttcgg
     2521 gaactggctg aaagcctgcc ccgcatactg ccggaaggtg gcccggacaa agctgcgctg
     2581 ttgcagcgac tggctaacga agaactggcc caggcagagt atgaagatca ggttcgcgcc
     2641 aaagtgaccg ccgcccgtgc tgacacgcgt ccgggtatga ccacggaaca actccgccag
     2701 cgcctgcatg gccgttatca ggaactgcgc gatgccgtat gacgtggaat ggaagcaggg
     2761 agccatcgag gatgtaacga cgctgtttga ctatatcgcg gaaaattcat ccctgtggga
     2821 tgcgcagaac gttaccgacc gggttctcgc tgcggcggac aaactggccg acttcccgcg
     2881 actctatgaa gtggactcac gctatggcga ggacgttcgc cggatcagtc tgatgggaca
     2941 gcatgtcctc tatgatgttg atgaccagac cagaaaagtc cgggttctgg ccgtggtcgg
     3001 ccagcgccag aatccccata ttgttctcta ataaccggtg acggggacgc aattcattgc
     3061 gtggccgtcg ccggttggac aagcacagcg cgtcagcgtt ttccccacgg ccaccaccgg
     3121 cgcggtgttc cggccggggt ctctgctgtc tccggtttgt gttccagcag tctcatggac
     3181 tgcatttcct gccgtagccc ggccacctcc tccctgagtg cttttatctc cgcgatcaac
     3241 gcgtcggagg ccattttttc agtctcaccc gacagtctca actcgtcccg ctcgtgagtc
     3301 tcatcctgtc ttaactcacc gtacacccgg ataagctcgc tggtatccac cacccggccg
     3361 ccatcagctt ccgtacggta tgatacgcgg cctttcgcca tatcccgata cagggaactt
     3421 cgggctttac ccgtcagttc cgtcgcctgt ctcaccgtca cccacgccat tgtctcaccc
     3481 cgctattttt gagacagtat acctgacagg cgcacaccat gcccggaagg aaagaatcat
     3541 gacaaaacag gaaaaaacag ccctcaatat ggctaaattt attaaggagc agtcgctgct
     3601 attgctcgat aaactgaatg agctggatat ggataccccg gccgacatgt gcgagcggct
     3661 acacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg aggaataaca
     3721 gcaccggcat ggccggtgct ggcatttatt ttttgccgaa cggccaccac cggcgggcag
     3781 gtttgtcctc cggcagttca ggtaccggca cgttaaaata gcggctgtta tattcccgca
     3841 tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctca ctcaccacgt
     3901 caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc tgcacaccgt
     3961 tctcgatggc gaaccgctcc accttgcgat agaggtaatc acacagcttc attcctgtgg
     4021 gtttgttccg ttcccattgc ggatttttac cagtgaaaaa gtacgtggct ttttcgtcgc
     4081 tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc tgggcccggt
     4141 cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccata tcttccagat
     4201 gctcgctggt cggcatgatg cagcgctcgc cgttccggtc ctccgcgtct tcaataaata
     4261 ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac gcaaacaacc
     4321 gttcacgatc cagctctgcc gttttgcggg cctcgccggt ctgtgggttc acgatttcat
     4381 ccaccgacag gattgcccgc tgcaccctga cctgcgtcgc ccggtccggg cagatgtacc
     4441 agatttcatc gatgccgtta gctttgcggt tgaacagata cccggacacc acttcctgat
     4501 accggcgacg gctcttgacg gtgcgctcca cctcaaacgc caccgtttta ccgtccagct
     4561 ccgccagggc gtccggcact ttcatgcctt taagactcag acggtgcaga atgcgccagc
     4621 cggtggcccc tctggcttcc atatcaaggc gcagggattg cacggccagc cggtgcggca
     4681 gatgtgctgc cgacaccctt gacggctgaa acggaataac atccgccagc ggttcgtcct
     4741 cgtcgaacga gaacgccacg ccatgcggcg tcagccccca cacgctgacc tgacggccgt
     4801 ccaccggata tttggtccgg accagcagcc ctttcgccac caggctgtcg aggaagcggt
     4861 agaccggctg caccgccgca atccccagcc gccgcgcaac cagctccgcc gtggaacaca
     4921 gctcttcccg caaccagcgc agggtcagcc gggttttctc cgcgttccgc gcctgtcgtt
     4981 tcgcgtggct gacgttgtac agctccagcg ccatcagtaa atccccgcaa aatcatcatc
     5041 cgctttcggc ccgccgccgt cggccggggg ccggaactcc gccaggggat cggttttcgc
     5101 cagcaccggg aaacgctgca acgggatttc ccggcgctca acccgcagcg gactgctgaa
     5161 cgccatccgg gccacaccca gcccggtcat gaccgcgaac ccgtccggca ggtgttgcag
     5221 ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtccc cgctgatatg
     5281 ctccacgttc cccgcctccc ggctggcacg gcggcgctcc acgtccaccc tgatttcccc
     5341 ggtctgcccc gccgcccact gcgcggactc caggctggcc gcccggtaaa accagcggat
     5401 cggcgtgtta tccagtaccg tggttttcac aaattcagcg ggcagatcct gcccgcactg
     5461 cccgaaatcg cccagcgact ggtgcgcgag caacagattg cagtcccggc tgcgcagcgt
     5521 ccccagtgcg ttcagtacat acttcgacag cagatattta atttcatcga gcatcacgct
     5581 gacgtgtggc cactcccgga actcgtcccg cgcaatgatg atttgcgcgc agcgggcaaa
     5641 cagcattttt tgcacacgga tcaccgcctc gtcgtccatc gagccgataa cgtacaggca
     5701 gccgccgccg ttgataatcc ccgccacatc gatgccactg tccgtctgca ggacgctcag
     5761 gtcagcgacg ttttccagcc tagtgatcaa ccccttcacg tcctttttca gggcctccgg
     5821 cagcgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggaa actgctccgc
     5881 aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt cgccggtctc
     5941 gcccaggtta agcccggcaa tcagcaggtt tttcagcgcg tactggtcga gatcgcggaa
     6001 cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt ccggacagtg
     6061 cgcccggaag acgtgcggcg cccatgcgtc ccctttgggg tcgaagtaca ccaccgcgtc
     6121 cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcacgctct tgccgctccc
     6181 gggcaggccg aggatctgaa tgttggtctt gcgccaggta tcacgcggaa ccagcaccgg
     6241 cttgccctgc tcatccttac ccagaaacac cgcctgctcc tgccgggcct gctccgagga
     6301 aaaatcggcc agcgcattaa ccggtaccgg ctccccgaaa tgcaggtggc gcacgtcggt
     6361 catctctcct gtccgctggc tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc
     6421 ctcgtcagca gccgcaaaat gaccgtcaag cagcaggaac gccagcagcc gcgccaacac
     6481 aatcccgaca gccagcccgc ccgcgcacca ggcaatcccg aacaggttag ccggctgcag
     6541 caggtaataa tctgcgtttg cgtagaggtc cggacgcagg acggtttctc cccagctctc
     6601 gataaacacg gcgacaacca gcccccccag aagggccaga ccggccgcgc cggtcagcca
     6661 gtacgcgtag ttccagacat gtcgacgtgc cctgaccatc cgcccgggcg aatccagcga
     6721 ggccagcacc gaaaatggcg ctggccgttt attatcgcgc ttatagcgat cggcatactc
     6781 ccgtcgctcc ttatgactca gccccacaaa acgcatccgt ccggcccaaa gttcagccag
     6841 accgaaaaac aggccgataa ctaggaaagc cccggccaga ccgtaatact cctggtacag
     6901 ccagctgacc cacggataaa tctgccgggc cagctccgga atgtgccact tccaggcaag
     6961 cgaatccgta ttaaacatgt tctctctcct tcagacgatg gtttgccggc gtgatgtgca
     7021 gcccccgctg cacaagccgc ggacatgccc caaggcatgg cggcggcggt cacgtccgtg
     7081 gtgattgcgt tcagctttca ccacggaggc cagcctcttt ccgccccatg cggaaaaggc
     7141 gcagccccgg aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag
     7201 tgcggtcttt tccggtgttc cggcccccgt cagagttcgc catagcgagc ggctgaccac
     7261 ccgcaaaatc aggagcagct ttgctgcttc gccaggtgcg tccagcaccg tggatttttg
     7321 tgggtggtca ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca
     7381 gccggacgga gccagaaaga gggggagaga ccgtgcgtcg gtggcgtcca gccagcgtaa
     7441 gcaggggata tccccctgag gtcagccacg ccctcgcggg gatgacctgt atttctccgt
     7501 aaggagaaaa aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgatatga
     7561 ttcacctatc ctggtaattt attatcaggg aatataaaga cttaatcagg ggacaatatg
     7621 ccacatcagc gcgtccggtg tccgtcttgt aggtgccggg gaaattgcgc ctcctcgctc
     7681 actcggtcgc tacgctccgg ccgtgagact gcggcgggcg ctgcggacac atgcaaagtt
     7741 acccacagat tccgtgaata agcaggggac taacatgtga ggcaaaacgg caaggccacg
     7801 caggtggcgt ttttccatag gctccgccct cctgactgag ttcacataaa cagacgcttt
     7861 ttcggtgcat ctgtgggagc cggtggctca accatgaatc agtcaggagt ggcgaaaccc
     7921 gacaggactt aaagatcatc accgtttccg atgaatcgct ccctcttgcg ctctcctgtt
     7981 ccaaccctgc cgtttcccgg atacctgttc cgcctttctc ccttgcggga agtgtggcgc
     8041 tttctcatag ctcacactct ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg
     8101 ctgtaagcaa gaaccccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact
     8161 tgagtccaac ccggaaaagc acgcaaaacg ccactggcag cagccattgg taactgggag
     8221 ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg
     8281 gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta ccacggttaa
     8341 gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt tttttcgttt
     8401 acagggcaaa agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8461 actgaaccgc gatccccgtc agttcagagg aggacgatgg tgccaccgtt ccaccctgaa
     8521 catcccggat atagtttgcc tgacatgcga ccagaagaaa gcctgaaaaa atcaatataa
     8581 aaagaaaaac aacctttacc tttttcatat accttccctg tatttaaaaa ccccggtatt
     8641 catccctgaa caccggaagt ctgtatttca aatcatcctt gataactttt gcttaccagt
     8701 taccatcccg gtaaacaaag gatacaaagt aatcaaattt actaatatcg attacatttt
     8761 taaagtgtgg ctgtaatatc gaaacccatg atttttcaac atcaaaccaa ccattattaa
     8821 tattatcctt taaaggcatc cccagacttt caatgacaga accatcatca ccgaagtctt
     8881 ttgagtattc accaccttta aactcttcgg ttttcttatc aaaccaatga atatgtaatt
     8941 taagccccat atttacctct taaagatatt ttttaatgtt tcgttttgga tccggccctt
     9001 taacctgctt acccgttttt ggatcgaatg ccccgaggtg ttcgccatca ctggcgcggt
     9061 acccttcaag ctcaccgtgc tgggaatccc attcataaat tttacgcttt ttatcaccat
     9121 accatcgggc ccgcttacca ccaccacctt gtttaggggt tttaggttta ccctctttca
     9181 actctcccaa ccccttaata tcttcagtct tgggatcagg aaaataatca tggccgtaat
     9241 ctttagttcc cttacgaggt tttttctttt cctcatttaa tttattttct gcat
//
