LOCUS       NZ_CP149504             1919 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain FDA strain Seattle 1946 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP149504
VERSION     NZ_CP149504.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38321313
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Hall,M.B. and Wick,R.
  TITLE     Benchmarking reveals superiority of deep learning variant callers on
            bacterial nanopore sequence data
  JOURNAL   bioRxiv (2024) In press
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Hall,M.B. and Wick,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2024) Department of Microbiology and Immunology,
            The University of Melbourne, 792 Elizabeth Street, Melbourne, VIC
            3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: 19-DEC-2023
            Assembly Method              :: Trycycler v. 0.5.1
            Assembly Name                :: ATCC_25922__202309
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 1702x
            Sequencing Technology        :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038098015.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 12:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP149504.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDA strain Seattle 1946"
                     /isolation_source="clinical isolate"
                     /culture_collection="DSM:1103"
                     /culture_collection="NCIB:12210"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
     gene            928..1164
                     /locus_tag="WJ042_RS00005"
                     /old_locus_tag="WJ042_25320"
     CDS             928..1164
                     /locus_tag="WJ042_RS00005"
                     /old_locus_tag="WJ042_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1366..1572
                     /locus_tag="WJ042_RS00010"
                     /old_locus_tag="WJ042_25325"
     CDS             1366..1572
                     /locus_tag="WJ042_RS00010"
                     /old_locus_tag="WJ042_25325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064408406.1"
                     /translation="MLITGVRVMLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYM
                     ENPTSANYKIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          591..764
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(103..624)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.308"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aactgcttaa ccaggtaact gggtatacga gaccgcagat accgaaactg ttctttcagt
       61 gtagccgcag tcaggcccat acttcaagac ctctgaatct ctctctcgta tatcccagtt
      121 accagtggct gctgccagtg gcgctttgtc gtgctttctc gggttggact caagatgata
      181 gttaccgggt aaggcgcagc agtcggactg aacggggggt ttgtgcatac agtccagctt
      241 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagataaa cgcggccata
      301 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
      361 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
      421 gtcaggtttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgcgg
      481 ccctatcgct tccctgttaa gtctcttcct ggcatcctcc aggaaatctc cgccccgttc
      541 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
      601 agcggaatat atcctgtatc acatattctg ctgacgcgcc ggtgccgcct tttttctcct
      661 gccacatgaa gcacttcact gacaccctca tccgtgccaa catagtaagc cagtatacac
      721 tccgctagcg ctgcgtgact ggttcagggc tgcgccccga aacccgctaa aaccactgcc
      781 gcgcctgcgg cttgcccaac gtctcacgtc accggattaa aatccgctcc cgttcaccgt
      841 ttctggctgt ctttcagcac cgccagcgct tcatcggttc tgctgtgtct ggcagactgt
      901 gcggagttat tctgacggga gaatgctatg aacagactga caaacctgac acctgcagag
      961 aaaaaatttc ttgatgatgc tattgccgca gctgagagag cgtcaggcaa aaagctaaat
     1021 cagccgaacc ggcatatcgt cctgaacaga gcgcgtgcgc agattgagtc gcagcgttac
     1081 gccgaccgcc agcgtgcatt acgtgaggat gagcggcagc agtccgagtt tgcctggtca
     1141 cgtccgcgcg cgccgcgccg ctgattgcgc cttttggcgg tttagtggtg ctgacactcc
     1201 gaaatcgtct gccattcacc cgcgtgcata gccatgcagg tacgcagaaa tcatcagggg
     1261 cgttttttct cgatatgggc acagaaaggt gtatgtttca ggccggaggc ctcagaaacg
     1321 cgctgagggc gtttgaggcg gtgcggccaa cggatgttat gtttaatgtt gataacagga
     1381 gtaagagtga tgcttacatt tattggtgta gtggtagtcg ccgttattgc atttaaatta
     1441 tttatgcttt tttttagttc atccgaatct gccgtcgaac gtgctacccg taaatatatg
     1501 gagaacccta cgagtgctaa ctataaaata atggtggcta cacgcatgag aaatgagaag
     1561 aaaagagggt gatatatgtt tgagtttatt ggttttttat ttttgctttt ggtgtgttat
     1621 gtctttatac aaatggttgc tgtcagggtg tttcctgaat atggagtacg gaaagcggag
     1681 gaacgatacc gtcgaaatcc atcagcagaa aatgaggatc tgttatggag agctagaagt
     1741 cgtataacta aacgcaagta atgtttccta agtgtgttga taggcaaaca tggtgggggt
     1801 tactactctc ggagttaatg atcttcttgt gatcctgtcg tttcagtcgt aatctcttgc
     1861 tctgaaaacg aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggg
//
