LOCUS       NZ_CP149460             3126 bp    DNA     circular CON 15-APR-2024
DEFINITION  Staphylococcus aureus strain BSN90 plasmid pUSA01, complete
            sequence.
ACCESSION   NZ_CP149460
VERSION     NZ_CP149460.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637678
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN90 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: FEB-2024
            Assembly Method              :: SPAdes v. FEB-2024
            Assembly Name                :: BSN90
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Reference-guided Assembly    :: CP022720, CP014442, CP080006
            Genome Coverage              :: 160.0x
            Sequencing Technology        :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038097895.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 12:43:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP149460.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN90"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pUSA01"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-07-30"
                     /collected_by="Andrew D. Berti"
     gene            1..996
                     /locus_tag="UXQ40_RS00005"
                     /old_locus_tag="UXQ40_13700"
     CDS             1..996
                     /locus_tag="UXQ40_RS00005"
                     /old_locus_tag="UXQ40_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1580..1768
                     /locus_tag="UXQ40_RS00010"
                     /old_locus_tag="UXQ40_13705"
     CDS             1580..1768
                     /locus_tag="UXQ40_RS00010"
                     /old_locus_tag="UXQ40_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341485400.1"
                     /translation="MNKNTLLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            1802..1924
                     /locus_tag="UXQ40_RS00015"
                     /old_locus_tag="UXQ40_13710"
     CDS             1802..1924
                     /locus_tag="UXQ40_RS00015"
                     /old_locus_tag="UXQ40_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000491229.1"
                     /translation="MFSHCFFIPIGVVAYFLFFYKDDAFDEMEEKYDYHEDNKK"
                     /name="hypothetical protein"
     gene            complement(2094..2675)
                     /locus_tag="UXQ40_RS00020"
                     /old_locus_tag="UXQ40_13715"
     CDS             complement(2094..2675)
                     /locus_tag="UXQ40_RS00020"
                     /old_locus_tag="UXQ40_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1067..1231
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          2..996
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatat
     1141 cactttaaaa taccttaaaa cccctggaat ttctggcttt gccagaccta tcatttttga
     1201 atgatagcaa attctcctta tgctcttacg gagtttttag agaaaaatta aaaattctcg
     1261 atttttgata aaaaacgccc tgcaggaatt tagaaaaaca tgtggaagtt ttaaaggatt
     1321 ttatgctaat ttttaatttg catgtaaatg ggcagtgtta aaaaattagt cactggtttt
     1381 gctgaaattt ttgtttgtga aattaccata tatttatttg gctcatattt gcgttttaag
     1441 agcttgtata tatttttaag ttaaaaacct atatgatacc cccctaaacc tttaaaatac
     1501 ccccttaaaa ctcaaaataa aggtatttat aatttgagta agtcttgtga taaagttttt
     1561 taaaaaagga gtgtttattt tgaataaaaa cacattgtta gatgaaggtt acgttatagc
     1621 aactatttta aatgttttct ttttacttgg attaattttt atatctcatt tggaaaattt
     1681 atatatatta attccgtatg ttattttgat gggaataaat gctatttatt tggttgttaa
     1741 ggttatgaat ttcaaaaaga ataattagaa tatttgaggg gattttgaaa cgagtttctt
     1801 cttgttttca cactgttttt ttattcctat tggtgttgtt gcttactttt tgtttttcta
     1861 taaagatgat gcttttgacg agatggaaga aaaatatgat tatcatgaag ataataaaaa
     1921 atagacgacg catttatgcc gagaaaattt attgatgttg agaagaaccc ttaactaaac
     1981 ttgcagacga atgtcggcat agcgtgagct attaagccga ccattcgaca agttttggga
     2041 ttgttaaggg ttccgaggct caacgtcaat aaagcaattg gaataaatag tatctaaagt
     2101 aacccgtatt taagattaca ttgtacatcc ttcgaaacca tagtagctgc gattccagct
     2161 aggtttcctt ttatcccaat tttagctaat ttttttgcta cgccctttaa gtttttggct
     2221 ttaacatctt caaaaactgc tgtgattatg tttccagtta aaagttctcc atatgagttt
     2281 aaaacttcag aatagaaaca gtcaccgcta tttttgtagt tcccaccaac tgctctaggt
     2341 gatattgaac tagttttctt tttattctct atgtcgtttt ttagtttttg gaatccttca
     2401 ggaacatatc catatcgaga tttggcattt tctagattaa catctttagc tgttccgtct
     2461 ttatctaaaa taataatttt gccatatatg tattttagtt ctttttcaat ttctttttcg
     2521 tttaaagttt caacgttttt atctgcaatg ttatttttgt tttgggcttg agcttctttg
     2581 aaattaaaat ccaagaccat tgaaagtatg gttaaaatac ttaaagatac tactaaaatc
     2641 tttcttagtg atatactgtt ttgaattgca ttcattgtaa atgccccctt gttttgttat
     2701 ttaaatgaat tcaatataat attaacatat aaaaacaaag gagtttaata tgaataagtt
     2761 tttttacttt ttgattattt tattatctgt atttttaatt tttaattttg ttaattcgat
     2821 tttatttgat aatccttttg actttttaga aagttttatt tattcattgg ttttcacatt
     2881 aatttattta ggtttagagg gtcataaaaa gaagaaggaa taggttgttt tttgaaacga
     2941 gtgtgaacga gtttcttctt gtcttgatac tatatagaaa taactcgatt ttatatatat
     3001 agctgtaact gttgatatta cagtgtttaa acgtgttttt gtgcgtgaaa ggaaaatttg
     3061 acaataaaaa accccagtta tattattaag gtgtcgaatc ttaaataata ctgggggtct
     3121 ttttat
//
