LOCUS       NZ_CP148586             2678 bp    DNA     circular CON 06-APR-2024
DEFINITION  Escherichia coli strain Z1322HEC0001 plasmid pZ1322HEC0001-4,
            complete sequence.
ACCESSION   NZ_CP148586
VERSION     NZ_CP148586.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441547
            Assembly: GCF_037920625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037920625.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 22:27:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,674
            Genes (coding)                    :: 4,533
            CDSs (with protein)               :: 4,533
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 56 of 141
            Pseudo Genes (incomplete)         :: 80 of 141
            Pseudo Genes (internal stop)      :: 35 of 141
            Pseudo Genes (multiple problems)  :: 31 of 141
            Pseudo Genes (short protein)      :: 4 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148586.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1322HEC0001"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1322HEC0001-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2022-08-12"
     gene            complement(join(1984..2678,1..31))
                     /locus_tag="WHU38_RS23940"
                     /old_locus_tag="WHU38_23940"
     CDS             complement(join(1984..2678,1..31))
                     /locus_tag="WHU38_RS23940"
                     /old_locus_tag="WHU38_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="WP_032202691.1"
                     /translation="MKIFNLNLIANISSTIRTHLPTLQKNIRPHPSPNLDVTSHHRVFQ
                     KDGIGLSQTGKSTQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPQNLRLDFYTKDNDF
                     TKGLSVLSEVNKRHTEAQTGLTLSLNISKDDLSFLAKARNIPQSKLEEEMKSQAIYRKE
                     CATGNEKIKNYALYYHDQAQNIIQRHQDGSGNQNIDIAFITDKNHKKHLSDIFKVINDS
                     GVQYDVIHFGACRVNRDEQ"
                     /name="putative adhesin"
     gene            complement(311..469)
                     /locus_tag="WHU38_RS23945"
                     /old_locus_tag="WHU38_23945"
     CDS             complement(311..469)
                     /locus_tag="WHU38_RS23945"
                     /old_locus_tag="WHU38_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005093880.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     gene            complement(957..1151)
                     /locus_tag="WHU38_RS23950"
                     /old_locus_tag="WHU38_23950"
     CDS             complement(957..1151)
                     /locus_tag="WHU38_RS23950"
                     /old_locus_tag="WHU38_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041639.1"
                     /translation="MNKQQQTVLNMAGFIKGQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1435..1758
                     /gene="mobA"
                     /locus_tag="WHU38_RS23955"
                     /old_locus_tag="WHU38_23955"
     CDS             1435..1758
                     /gene="mobA"
                     /locus_tag="WHU38_RS23955"
                     /old_locus_tag="WHU38_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          1187..1426
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.347"
                     /coverage="100.0"
                     /evalue="3.58e-116"
     Typing          complement(398..928)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.327"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1436..1755
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="9.47e-63"
ORIGIN
        1 ttgctattaa atttaaatta aatattttca tttattaaca atccattttg cggtgttttt
       61 tttcttttgt ttaagggcgt gtcggtgtaa gtatttattt tatgggttaa agtaggatac
      121 caagtgtgaa aaaaaattaa aggttcttct tgagatcctt tttttctgcg cgtaatctgc
      181 tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt cgaaggttaa gtcagttggg
      241 gactgcttaa ccgggtaact ggcttggtgg agcacagata ccaaatactg tccttctagt
      301 gtagccgcag ttaggccacc acttcaagaa ctcttaatat ctcaatccac cttgtccagt
      361 taccaatggc tgctgccagt ggcgttaagt cgtgtcttac cgggttggac tcaagacgat
      421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      481 tggagcgaac gacctacacc gggccgagat accaacagcg tgagctatga gaaagcgcca
      541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      721 aaaatgccac ctgcgcggcc tttgtccctg ccttcggctt tttgctcaca tgttctttcc
      781 ggcgttatcc cctgattctg tggataaccg cattaccgcc tttgagtgag ctgacaccgc
      841 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aagagcgcct
      901 tatgtgacat tttctcctta cgctctgttg tgccgttcgg catcctgtcc tgagcgttat
      961 ctctctgtgc tattttctac ttcaaagcgt gtctgtatgc tgttctggag ttcttctgcg
     1021 agttcgtgca gcttctcaca catggtggcc tgctcgtcag catcgagtgc gtccagtttt
     1081 tcgagcagcg tcaggctctg gccttttatg aatcccgcca tgttgagtac ggtttgctgc
     1141 tgcttgttca tcttttcgtt ttctccgttc tgtctgtcat ctgcgttatg tgattatatc
     1201 gcgtaccact tttcgactgt tttaccgccg gtattctgcc gtctgacgct ttgacgggta
     1261 tttctgcctg acaatactgt cactgccaaa aaactgccgt gcctttgtcg gtaattcgag
     1321 cttgctgaca ggacaggatg tgcaattgtt ataccgcgca tgcatgcacg ctattacaat
     1381 taccctggtc agggctttgc cccgacaccc atgtcagata cggagccatg ttttatgaca
     1441 aaacgaagtg gaagtaatac gcgcaggcgg gctatcagtc gccctgttcg tctgacggca
     1501 gaagaagacc aggaaatcag aaaaagggct gctgaatgcg gcaagaccgt ttccggtttt
     1561 ttacgggcgg cagctctcgg taagaaagtt aactcactga ctgatgatcg ggtactgaaa
     1621 gaagttatga gactgggggc gttacagaaa aaactcttta tcgacggcaa gcgtgtcggg
     1681 gacagggaat atgcggaggt gctgatcgct attacggagt atcaccgtgc cctgttatcc
     1741 aggcttatgg cagattagct tcccggagag aaaactgtcg aaaacagacg gtatgaacac
     1801 cgtaagctcc caaagtgatc accattcgct ttcatgcata gctatgcagc gagctgaaaa
     1861 cgatcctgac gcatccttcc tgtttttccg gggtaaaaca tctctttttg cggtgtctgc
     1921 gtcagaatcg cgttcagcgc gtttcagtgg tgcgtacaat taagggatta tggtaaatga
     1981 tgattattgc tcatccctat ttactcgaca agcaccaaaa tgaattacat catattgtac
     2041 tcctgaatca tttattactt taaatatatc ggaaaggtgt tttttatgat ttttatcagt
     2101 tataaatgct atgtcaatgt tttgatttcc tgaaccatct tgatgtcttt gtataatgtt
     2161 ttgagcttgg tcatgataat atagagcata attttttatc ttctcattac ctgttgcgca
     2221 ttcttttcta tatatagctt gtgatttcat ttcttcttct aattttgatt gaggtatatt
     2281 gcgcgctttc gccagaaagc ttaggtcatc tttgcttatg tttagtgata gtgttagccc
     2341 tgtttgtgct tcagtatggc gtttgtttac ctctgaaaga acgcttagtc cctttgtgaa
     2401 gtcgttgtct ttagtataaa agtcaagcct taggttttgg ggaactacag tccatccatc
     2461 gcctttttga cgtgtgaata gtgtttgtgg atgggagatc tctttccatc caccgtgcga
     2521 cataataaca agttgtgttg attttcctgt ttggctcaat cctattccgt ctttctggaa
     2581 tacacgatga tgacttgtta cgtcgaggtt tggtgatgga tgtggtctta tatttttttg
     2641 tagtgtcggg aggtgggttc ttattgttga tgatatgt
//
