LOCUS       NZ_CP148514             2309 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0001 plasmid pZ1323CEC0001-8,
            complete sequence.
ACCESSION   NZ_CP148514
VERSION     NZ_CP148514.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441568
            Assembly: GCF_037576255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037576255.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:25:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,818
            CDSs (total)                      :: 5,691
            Genes (coding)                    :: 5,374
            CDSs (with protein)               :: 5,374
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 107 of 317
            Pseudo Genes (incomplete)         :: 205 of 317
            Pseudo Genes (internal stop)      :: 51 of 317
            Pseudo Genes (multiple problems)  :: 44 of 317
            Pseudo Genes (short protein)      :: 4 of 317
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148514.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0001"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0001-8"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-23"
     gene            complement(join(2127..2309,1..348))
                     /locus_tag="WHU28_RS29055"
                     /old_locus_tag="WHU28_29050"
     CDS             complement(join(2127..2309,1..348))
                     /locus_tag="WHU28_RS29055"
                     /old_locus_tag="WHU28_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000012591.1"
                     /translation="MSGFIPFVGSKYENSIYGLKLLVLGLSHYGDADDNYPDFTKDVIA
                     ENAYTTGNRFFTILTNVLRLSRESITDDQRKITWENIAFYNYIQQIVGEKGRISPTKEM
                     WDSAREPFLSVIKQLQPNVILVLGTELWDHLPEVDGVEWCWIRHPSSSNFSYDQAFNNL
                     KKSIGMENIVYLP"
                     /name="hypothetical protein"
     gene            complement(1179..1373)
                     /locus_tag="WHU28_RS29060"
                     /old_locus_tag="WHU28_29055"
     CDS             complement(1179..1373)
                     /locus_tag="WHU28_RS29060"
                     /old_locus_tag="WHU28_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1024..1106
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.81"
                     /coverage="100.0"
                     /evalue="1.76e-34"
     Typing          1410..1508
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1633..1888
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
ORIGIN
        1 aattacactt aggaatggct ctcgggcact atcccacatt tctttagtgg gagatattcg
       61 acctttttct ccaacaattt gttgtatgta gttgtagaac gctatgtttt cccaagttat
      121 cttcctttga tcatcagtta tgctttccct agacaatcga agtacattag tcagtatcgt
      181 gaagaatcgg ttacctgttg tataggcatt ctctgcaata acatccttag taaaatctgg
      241 atagttatca tcagcatcac cataatgcga caaacctaag accagtaatt ttaacccata
      301 gatactgttc tcatacttgc taccgacaaa tggtatgaaa ccactcatgg catactcctt
      361 tcttcggggt gatggttgtt ttttgaacat aaaaacctaa tgcccccgtg gtgggtatat
      421 aaaaaaccac ggggagcgga gcgaccttcg tccagctctt ttcttgtgtg tctcatcttg
      481 caacggattc gatttgtgtg gggttagtgt cagacttctc gcaatcacac tttcccgctt
      541 actgctgcct tattcgctca ttttatcagg tgcttgttat cgtacagcga gacggggtag
      601 agcctaactc tttcgcggtt aagttacgtt cccaccaatt tgcctgttta tcctgccgga
      661 gtccggggcg tagacctgag cgtctaaggt gcagatatga ttttaatgtc gttgaatggt
      721 attaagctgg tagtgacatg gctagccagc cctgctaaga tttagattgc tacgtctgat
      781 cttggcaggt atctgcaaag aattagagta ccgaagccct gtgattctgt cacggggctt
      841 ttgtacattt agcgcatgaa gatcattttg atcatgtcag atcgttttca ccgctcgccg
      901 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagtgc gcctgatgcg
      961 gcattttctc cttacgcatc tgtgcggtat ttcacacccg gcatggggca cttttcatac
     1021 aatctgcatt gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     1081 gtcagggctt cgccccgaca cccgctgacg cgccctggcg ggcttgtctg ctgccgaaaa
     1141 cgctcgcgga caagccgtga gcgcctccgt cagcttcgtt atgttcctgt ttcactttcg
     1201 gtctcaaagc gaacctggat gctgttctgg agttcttccg cgagttcgtg cagtcgttcg
     1261 cacatggccg cctgttcgtc ggcatccaga gcatccagtt tttcaagcag tgtcaggctc
     1321 tggcttttga taaatcgcgc catattcagc gcagtttgtt gctgtttgtt caccttccgt
     1381 tgttctccgt cagtaccggg tgtcgggtgg accctgacca ggtggtaatt gtaagacgtt
     1441 gcgggcgacg gtattacaat tgcacatcct gtcctgtttt tttaggacgt atcatggcac
     1501 agcatatcaa ctgataaata atccctgtgc agtgcagccg ctgttgcata aaatttcgct
     1561 ctgcgtcgag tgtttcttaa tcaataattc cgctgatact ccggaaaaac gcgagtctgt
     1621 tctcatgcct gaaatgccca caccccacgc aaaaacaatt ttttgctgat ttttctttat
     1681 aaatagagag ttatgacaaa ttagttcttc ttgctctctt tgtgatattt aaaaaagcgg
     1741 tgtcggcgcg gtgttgtagc tgcgccaaca ccgcttttta ggggtggtac tgactatttt
     1801 cataaaaaac atcattttat attaggggtg ctgctagcgg cgcggcgtgt ttttttacag
     1861 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
     1921 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac cgtatgcgcc caaaccgatc
     1981 gccattcact ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
     2041 ggttttcgct tggtaaaaca tctctttttg cggtgttcgc ggcagaatcg cgttcagcgc
     2101 gttttagcgg tgcgcgtaat gcgacgttat ggtaaataaa cgatattttc catgcctatt
     2161 gattttttga ggttattaaa tgcttgatca tatgagaaat tactagagct tggatgtcta
     2221 atccaacacc attcaacacc atcaacttca ggaagatgat cccaaagttc agtacctaga
     2281 acgaggatca catttggttg gagttgctt
//
