LOCUS       NZ_CP148503             3373 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0002 plasmid pZ1323CEC0002-6,
            complete sequence.
ACCESSION   NZ_CP148503
VERSION     NZ_CP148503.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441569
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096985.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:32:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148503.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0002"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0002-6"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-23"
     gene            complement(331..522)
                     /locus_tag="WHU64_RS00005"
                     /old_locus_tag="WHU64_27470"
     CDS             complement(331..522)
                     /locus_tag="WHU64_RS00005"
                     /old_locus_tag="WHU64_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            586..909
                     /locus_tag="WHU64_RS00010"
                     /old_locus_tag="WHU64_27475"
     CDS             586..909
                     /locus_tag="WHU64_RS00010"
                     /old_locus_tag="WHU64_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1118..>1555
                     /locus_tag="WHU64_RS00015"
                     /old_locus_tag="WHU64_27480"
                     /pseudo=""
     CDS             1118..>1555
                     /locus_tag="WHU64_RS00015"
                     /old_locus_tag="WHU64_27480"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1587..2147
                     /locus_tag="WHU64_RS00020"
                     /old_locus_tag="WHU64_27485"
     CDS             1587..2147
                     /locus_tag="WHU64_RS00020"
                     /old_locus_tag="WHU64_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_227316847.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGGWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2154..2387
                     /locus_tag="WHU64_RS00025"
                     /old_locus_tag="WHU64_27490"
     CDS             2154..2387
                     /locus_tag="WHU64_RS00025"
                     /old_locus_tag="WHU64_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2561..2695
                     /locus_tag="WHU64_RS00030"
     CDS             2561..2695
                     /locus_tag="WHU64_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484593.1"
                     /translation="MQHPENAFHAFLGIQPGENMVFLLFERKDVKNLVIALAIFRRWA"
                     /name="hypothetical protein"
     gene            2686..2817
                     /locus_tag="WHU64_RS00035"
     CDS             2686..2817
                     /locus_tag="WHU64_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484592.1"
                     /translation="MGVERKQLARKASCFGFIEVSDLLNVPVETVEIHNRNQFIQFA"
                     /name="hypothetical protein"
     Typing          167..250
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="95.0"
                     /evalue="4.11e-16"
     Typing          900..2470
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgaacgtag tgagcgagtg ggcgaggaag cggaagagtc acagaggcaa gcattgtgca
       61 cttacgcgcc tgtgccagtg tccgcgtgtg tgttgtttat cgggcgcgct gtttttacgc
      121 atctgtgcgg ctttttactc ctgacatggt gcacttccca tccactctgc accgatgcaa
      181 aatgaggaaa gccagtatac acatcgctat cgctctgtga actgggtcag ggctgcgccc
      241 cgacacccgc actgacgcgc cctggcgggc ttgtctgctc ccggcatccg ctcacaggca
      301 agctgtgaac gtctccggga gctgcatgtt tcagttgctt tcaccgtcat cgccgaagcg
      361 tgcgaggcaa ctgcggtaga gttcgtcggc gtggtcgtga agtgattcgc agatgtctgc
      421 ctgttcgtcc gcgtccagat cgttgagttt ttccagcagg gttagcgtct ggtttctgat
      481 aaagcgagcc atgttaaggg ctgttttttc ctgtttggtc atggtcttaa ggctccttgt
      541 atgggggatt tgttaggggg aatgataccg atgaaacgcg agaagatgct cacgatacgg
      601 gttaccgatg aggagcacgc ccggttactg gaacgctgcg agggtaaacg actggcggaa
      661 tggatgcggc gggtctgtct gggggagccg gttgcacgca caggaaaact gccgacactg
      721 gcaccgccat tgctgcggca tctggctgcc atcgggaaca acctgaacca gacagcccgg
      781 aaggtgaaca gcggccagtg gtcttcgatt gaccgggttc atgtggtggc cgcactgatg
      841 gccatcgagg gtgaattgcg ccagttgcgg caggctgtca gggaacaggg ggtacgggat
      901 gatagttaaa tttcatgcca gggggaaagg cggtggaagt ggcccggtcg attacctgct
      961 ggggcgggag cgtaaccgcg aaggcgcaac ggtgctccgg ggtaacccgg aagaagtgcg
     1021 ggaactcatt gattccacgc cattttcgaa gaaatacacg tcaggcgttc tgtcgttcgc
     1081 ggagaaggag ctaccaccgg gtgaacgcga aagagtgatg acgagctttg agcgtgttct
     1141 tatgcccggt ctcgaaaaag accagtacag cattctctgg gtggaacacc aggataaggg
     1201 gcggctcgaa ctgaattttg tcatcccgaa catggagcta cagagcggta agcgcctcca
     1261 gccgtactac gaccgtgcgg acaggccgag gataaatgcc tggcagacgc tggttaatca
     1321 tcattacggg ctgcgtgacc cgaacgcacc ggagaacaga cggacgcttg tcacgccaaa
     1381 taatctgcca aaagcgaaac aggaggccgc agaagcgatt acacggggtc ttgaggctct
     1441 ttaccatgcc ggagcaataa aaacgcgtca ggatgtcaca gaggcgctta cagcggcagg
     1501 ttttgaggtg gtgaggacaa ccaaaagcag catcagtatt gccgacccgg agggggggcg
     1561 gaatctcagg ctaaaggggg cgctgtatga gcaatctttt acaaatggga acagacttcg
     1621 agaagaaact gaaagagcga gccgcttcta ccgagaacat gctgaacagc gagttcagga
     1681 agctcgaaga gtctgtagac aaggcattga gcttaaacag gcagaaaatc agagacgcta
     1741 tcagcgaaca tacaacatcc gtgaaaaagc aactggatac gctgagcacc accgtgagca
     1801 tgcagttcag caccacagag gccgaactgt cccggcagca gaaaaaactg ctctggcggg
     1861 tgatcaaagg gcggatactg tttccggcgc tgaccgcgct cagcgtcacg ggcgggattt
     1921 tcctgggggg ctgggggctg atggagtggc aggaaagcaa gattgcgaaa aatatcctga
     1981 ctatcagaga gcaggaaaac acgctggcga aactcgaggc aaaaacctgg ggggtgacgt
     2041 tcgtcaatgg cgagaacggg aaatttctgg tgttgccgga cggggtgaaa ggggaaaaca
     2101 cctggacggt gggcgacaag aacgccgtga ggctggtcag ggagtaaacg agtatgacgg
     2161 aactggaagg gcatttgctg aacgccttag agcatttgca acaggactat atgcggcggc
     2221 tgaacgagtg ggagagcgcc ttcgcggaat tgcagaagat gcacgaggtt acgcaacgga
     2281 acaacgcgat actgaacgag cgcgtcgtga tattgagtca gcaggtgcag agattagccg
     2341 ggcaagtgga tcgcttgagt cggttattta tcgcgaacaa cagctaaggg atgaacagga
     2401 gcgtgcccgg tacagacatg aggtacagga acgcgcggca cgggttcgga gtgggccatc
     2461 aatggagatg tgaccggaga agtgacgaaa tcttgccatt ttgacccgtt cgacggctgg
     2521 ggcgagcgaa acaaatcgtg tgccgtattt gtgcatagct atgcaacatc ctgaaaacgc
     2581 atttcacgcg tttttgggga ttcagccagg ggaaaacatg gtttttttac tttttgagcg
     2641 taaagacgtg aaaaatctcg ttattgcact tgcgatcttt cgtcggtggg cgtagaacga
     2701 aaacagctcg ccaggaaggc gagctgtttc gggttcattg aggtctctga cctcctcaat
     2761 gttcctgtgg aaactgttga gatccacaac agaaaccagt tcattcagtt tgcatgagcc
     2821 ttctgtattc ttcaagagtt tttcaacagc acagtctccc aggaaccccg aaaaaaacct
     2881 gtttttgcag atacttgcgt atttgcgaga atcgtttgcg gttgggggag ctgtgacgac
     2941 ggcgaccgca cgcgtgagag ttccgctacc ggcgcagaga gtttcatcga cagcggacac
     3001 ctgatgagtt gccacaaaaa ccgtatgtag cgcggctaga ttggttgagt tagaccttgg
     3061 cggcagttcc cggtatgtgg tggcgtgacg ggtgagatgg tgcggcgaag acgcatacgt
     3121 cacgcaggcg cgggttcttc cactgacgcg ccccgtgatc gatggccttg gctccgtccg
     3181 gcatatcgag agcggcaaac tacccgtccc ttcactggga ggggtgagtt gttgccgttc
     3241 cctccgacct tccaccatcc agcatatcgc acgatcccat gtgcaccgct actcctgata
     3301 actccagtgc aaaaaaaaaa acggatctgc atcacaacga gagtgatccc accgctcgcc
     3361 gcagccccag cga
//
