LOCUS       NZ_CP148501             5650 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0002 plasmid pZ1323CEC0002-4,
            complete sequence.
ACCESSION   NZ_CP148501
VERSION     NZ_CP148501.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441569
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096965.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:35:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148501.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0002"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0002-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-23"
     gene            complement(442..633)
                     /locus_tag="WHU64_RS00005"
                     /old_locus_tag="WHU64_27415"
     CDS             complement(442..633)
                     /locus_tag="WHU64_RS00005"
                     /old_locus_tag="WHU64_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_059339183.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            698..1021
                     /locus_tag="WHU64_RS00010"
                     /old_locus_tag="WHU64_27420"
     CDS             698..1021
                     /locus_tag="WHU64_RS00010"
                     /old_locus_tag="WHU64_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1230..>1661
                     /locus_tag="WHU64_RS00015"
                     /old_locus_tag="WHU64_27425"
                     /pseudo=""
     CDS             1230..>1661
                     /locus_tag="WHU64_RS00015"
                     /old_locus_tag="WHU64_27425"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1699..2217
                     /locus_tag="WHU64_RS00020"
                     /old_locus_tag="WHU64_27430"
     CDS             1699..2217
                     /locus_tag="WHU64_RS00020"
                     /old_locus_tag="WHU64_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_089697312.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2224..2457
                     /locus_tag="WHU64_RS00025"
                     /old_locus_tag="WHU64_27435"
     CDS             2224..2457
                     /locus_tag="WHU64_RS00025"
                     /old_locus_tag="WHU64_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137438.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2820..4067)
                     /locus_tag="WHU64_RS00030"
                     /old_locus_tag="WHU64_27440"
     CDS             complement(2820..4067)
                     /locus_tag="WHU64_RS00030"
                     /old_locus_tag="WHU64_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_000589506.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHNLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLEKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDESYDCSDWFKQR
                     LEDIFEGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIIFMNLLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4064..4861)
                     /locus_tag="WHU64_RS00035"
                     /old_locus_tag="WHU64_27445"
     CDS             complement(4064..4861)
                     /locus_tag="WHU64_RS00035"
                     /old_locus_tag="WHU64_27445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484606.1"
                     /translation="MVLSDIGGERLHRFFKDLDKIYPDRIMEFNIGNSSYSYQFNSNRA
                     WTTKNLNVEKSRLLQPVSDFTREMLDVCMLILWGGNTINNTNLPTEIENNCVLGNLIGN
                     KEAIEQFVKERYIMVSRQTGGATANDLGHICEIFIKEKLYKLIDNNISLDGHHIDGVTH
                     NDKDLTTFDIVAKNTTTKKCVGIEISFQVTTNSTIERKAGLAKSRRDLLNSRGHKIAYV
                     IDGSGNFQRSNAIKTIINHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            4881..5090
                     /locus_tag="WHU64_RS00040"
     CDS             4881..5090
                     /locus_tag="WHU64_RS00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252257226.1"
                     /translation="MGDILLHNRTASKQDVGLFVDIVSIDKNSSWVSIRGLMALIDAAL
                     SASSLGQYKHALFSNSWNSMIIPW"
                     /name="hypothetical protein"
     Typing          280..364
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          complement(3..269)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.37"
                     /coverage="99.0"
                     /evalue="4.24e-94"
     Typing          complement(5303..5645)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.812"
                     /coverage="99.0"
                     /evalue="1.76e-161"
     Typing          666..1015
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.35e-67"
     Typing          1012..2561
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.84"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcggcgt ggccttttcc
       61 gggttcttgt tttttgctca catgtttcct ttggcgttat cccctgattc tgtggataac
      121 cgcattaccg tctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgtagc
      181 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
      241 tgcggcattt cacacccggc atggggcact tttcatacaa tctgcactga tgccgcatag
      301 ttaagccagt atacactccg ctagcgctac gtgactggtt cagggcttcg ccccgaaacc
      361 ccctgacgcg ccctgacggg cttgtctgct cccggcatcc gctcacagac aagctgtgac
      421 cgtctccggg agctgtatgt gtcagaggtt ttcaccgtca tccccgaagc gtgcgaggca
      481 gctgcggtaa agctcgtcgg cgtggtcgtg aagtgattca caaatatcgg cctgttcatc
      541 tgcgtccagt tcgttgagtt tctccagcag cgttaatgtc tggcttctga taaagcgggc
      601 catgttaatg gcggtttttt cctgtttagt cactgatgcc tccgtgtaag ggggatttct
      661 gttttatggg gtaatgatac cgatgaaacg cgagaggatg ctcacaatac gggttactga
      721 tgatgaacat gcccggttac ttgaacgctg tgagggtaaa cagctggcgg tatggatgcg
      781 gcgggtctgc ctgggggagc cggttgcccg ttccgggaaa ctgccgacac tggcaccgcc
      841 gttactgcgt cagctggccg ccatcgggaa taacctgaat cagacagcga ggaaggtgaa
      901 cagcgggcag tggtcttccg gtgatcgggt tcaggtgttg gctgcgctga tggccatcgg
      961 ggaggagctg cgtcggctgc gtctggccgt cagggaacag ggcgcgcggg atgattgtta
     1021 aatttcatgc caggggaaaa ggcggtggca gtggtccggt tgattacctg ctgggaaggg
     1081 agcgaaaccg ggaaggggca acggtgcttc agggtaatcc ggaagaagtc cgggaactca
     1141 tcgatgccac gccatttgcg aagaaataca cgtcaggtgt tctgtcgttc gcggagaagg
     1201 agctgccgcc gggaggacgt gaaaaggtga tggcgagctt tgagcgtgta ctgatgcccg
     1261 gtctcgaaaa gaatcagtac agcatcctgt gggtggagca ccaggacaag ggacggcttg
     1321 agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc cagccgtact
     1381 acgaccgggc agacaggccg agaattgatg cctggcagac gctggtgaat caccattacg
     1441 ggctgcatga ccccaacgcc ccggagaacc accggatact gactctccct gataacctgc
     1501 cggaaacgaa acaggcgctt gcagagagcg tcacacgcgg gatagacgct ctttatcatg
     1561 tcggggagat aaaaggccgt caggatgtga ttcaggcgct cacagaggca gggcttgaag
     1621 tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaacggtggg aagaatatca
     1681 ggctgaaagg agcattctat gagcaatctt ttacagacgg gcgcggagtt cgagaaaaag
     1741 ctgaaagaga gagccgaatc tacagagaca atgctgaacg acgagttcag caagctcgaa
     1801 aaatctgtaa gcaaggctgt gacctcaaac gagacgaaaa tcaaagacgc tatagccctg
     1861 ttcacagctt cgacagagga atcactgaaa aaacaccggg aaggggtgaa agaggcgatg
     1921 atgcagcaca ggaaggacgt gttaaagctg gcagggaata cgggcatgat gttactgggg
     1981 atggtcattt tcctgtttac cgtgagtggc gggacgctct ggtatcttgg cgggcggata
     2041 cagacgaacc tggaagaaat caggaagcag gaagagacat tgcagaaact gaacgcgaag
     2101 acatggggcg tggagtttgt gcaggacggg agaaggaaat ttctggtcat cccgcagggg
     2161 aaatcggcaa cggtgattcc ctatcagggg aaggactggg tacaactgac ggagtgacac
     2221 agagtgacgg agctggaaag acatttgctg aacgcattag agcaactgca acaggactat
     2281 atgcagcggc tgaacgaatg ggagagcgcc ttcgtggaat tgcagaaaat gttttcgctt
     2341 acgcaacggg acaacgcgat gctgaacgag caggtcatgc agttgagtca gcaggtgcag
     2401 cacttgagcg agcggacaga acgcttgagc cagttataca gcgagaacag gagataaggg
     2461 acgaacggct gatacaggaa cgcgaacatg agttatccct ggagcgggaa cgccagccgg
     2521 aaatacagga acgcacactg gatggtcctt cactgggatg gtgaaactat gaaaaatggc
     2581 aggttaagtg gattaagacg ggctaatgtg gtctgtacca tctggttgca taggtattca
     2641 tgcggttaaa atttatcagg cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt
     2701 tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgcgta atgcgacgtt
     2761 atggtaaatc tgttaaattt aattaaataa ccatagagca gttttgctag taatatggtt
     2821 tatataaagt tattcttcat tatatcaaga aaaatatttt taatgactgg tacgcttaca
     2881 gcattcccta attgtttata tgccatgcta tcttttgggt gtaagatata tgagtctggg
     2941 taaccttgta gtcttgcgca ttctcttggc gtaaggcgtc gcagtctatt gttgtgatag
     3001 actccaagtc tattggcatc agatgccgta agtgtaattg acactttatt tgggtcaagg
     3061 aatttgaata cttcaaaaga catatttccg caaactgggt tgtatagtcc gtttttctct
     3121 ttaaggtaac cctttttaat taggtttttt attatgttgg tggtttcgct atgtttaaaa
     3181 aatgtttcaa tttgtgcttt tgacaaagcc ttgccgtctt ggtgtgtgcc aaaaatatgc
     3241 tttctacggt ttgcaattaa taaattcatg aaaattattt catcatttga gcactcgcct
     3301 tttagcccaa gatcccaaga gtgcaaggag tttccgttcc ttgtgtcaat tagcctgatt
     3361 ccatgaactt tatttaggtc accttcaaaa atgtcttcaa ggcgttgctt gaaccaatcg
     3421 ctgcaatcat atgactcgtc tggactatcc tctagtatgt cagaaacttt tgattctgta
     3481 taaaaatcat gaccaccttt ggatggtttg taattgtgcg tatctttggc tccaagatca
     3541 ttaggaatag taagtgttac ctctttttct aatgaggcta ttatatatat tctaacccta
     3601 ttttgaggga caccaaaatt acttgtgttt aatagtctgt attcaacatg ataccctaaa
     3661 ttatgaagag attttaatat tgttgaaaat gttcttcctt tatcatgtga agttaatcca
     3721 cgcacattct ccaaaagaca taacttaggc ttgtttttct ttatgattct ttctacttca
     3781 aaaaataatg tgcctcgggt gtctgcaaaa ccaagttgtt ttccagcata agagaacgcc
     3841 tgacaaggaa atcctgccaa taaaacatcg aagctttcat ctatatcaac atcatgtata
     3901 tcacaatatg attcttcgtc gaagttaagt ttgtatgttt cgcatgcttt tttgtcaatt
     3961 tctgagcttt ttacgcattt tgttgacaat ccaatttctt tcatggcttg ctcaaaacca
     4021 agtctaatac cacctgtgcc agaaaaaagg tcaacaaatt taatcatttc aatgtctcgc
     4081 agatgaatgt tgctagtgaa tttataccat tatctgaaaa gttaacggtc atgtcactat
     4141 ggttaattat tgttttgatg gcatttgaac gctgaaaatt accagaccca tcaatcacat
     4201 atgctatttt atgtcctctt gaattgagca aatctcttct gcttttggct aatcctgctt
     4261 ttctttctat tgttgagttt gttgtaactt gaaaacttat ttcaatacca acacattttt
     4321 ttgttgttgt gttttttgct acaatatcaa aagtcgtaag atctttatcg ttatgagtta
     4381 caccatcgat atggtgtcca tctagagata tattgttatc aataagcttg taaagttttt
     4441 cttttataaa gatctcgcat atgtgtccaa gatcatttgc cgttgcacct cctgtttgcc
     4501 tgctaaccat tatatatctt tcttttacaa actgttctat tgcttctttg tttccaatta
     4561 aattccccaa aacacagtta ttttctattt cggttggaag atttgtattg tttatagtat
     4621 tacctcccca taggatcagc atgcaaacat caagcatttc acgtgtaaaa tcacttacag
     4681 gttgcaagag ccgtgatttt tccacgttta aattcttggt tgtccatgct ctattgctat
     4741 tgaattggta agagtaagag ctatttccta tattgaactc cattattctg tcggggtaga
     4801 tcttatctaa atctttaaag aagcgatgaa gtctttcgcc tccaatatct gacaaaacca
     4861 ttaagtgctt gacaaatagg ttgggtgata tcttattgca taaccgtacg gcatcaaaac
     4921 aagatgtagg cttatttgtt gatattgtta gtattgataa aaattcatct tgggtctcta
     4981 taagagggct tatggcgcta atagatgctg ctctttctgc aagttctttg ggccaatata
     5041 agcatgcgct attttctaat tcttggaact ccatgattat tccttggtaa aactatccaa
     5101 atagaccttg agttacaccc ctatgatggg taatctctac gggcgtaaac gaaaaaaccc
     5161 acgttctcag gcgtgggttt ttcgaaggtt agaagtttct caggctttct aacccaaggt
     5221 aacagggcgt acaagaccgc tgccaccaaa tctgtccttt cagtgtagcc gcagtcgggc
     5281 cttcacttca agaactccgt atcagcaatt tcttgtacac cctctaccaa tggctgctga
     5341 cagtggcgtt aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacggccta
     5461 caccgagccg agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5641 gcgtcgattt
//
