LOCUS       NZ_CP148497             3675 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0003 plasmid pZ1323CEC0003-5,
            complete sequence.
ACCESSION   NZ_CP148497
VERSION     NZ_CP148497.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441570
            Assembly: GCF_037575825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037575825.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:34:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,770
            CDSs (total)                      :: 5,640
            Genes (coding)                    :: 5,270
            CDSs (with protein)               :: 5,270
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 114 of 370
            Pseudo Genes (incomplete)         :: 285 of 370
            Pseudo Genes (internal stop)      :: 63 of 370
            Pseudo Genes (multiple problems)  :: 76 of 370
            Pseudo Genes (short protein)      :: 4 of 370
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148497.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0003"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0003-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-23"
     gene            join(3433..3675,1..78)
                     /locus_tag="WHU08_RS28825"
                     /old_locus_tag="WHU08_28825"
     CDS             join(3433..3675,1..78)
                     /locus_tag="WHU08_RS28825"
                     /old_locus_tag="WHU08_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_042863636.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            287..>718
                     /locus_tag="WHU08_RS28830"
                     /old_locus_tag="WHU08_28830"
                     /pseudo=""
     CDS             287..>718
                     /locus_tag="WHU08_RS28830"
                     /old_locus_tag="WHU08_28830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            756..1241
                     /locus_tag="WHU08_RS28835"
                     /old_locus_tag="WHU08_28835"
     CDS             756..1241
                     /locus_tag="WHU08_RS28835"
                     /old_locus_tag="WHU08_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_042863634.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYLTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGKEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1245..1457
                     /locus_tag="WHU08_RS28840"
                     /old_locus_tag="WHU08_28840"
     CDS             1245..1457
                     /locus_tag="WHU08_RS28840"
                     /old_locus_tag="WHU08_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1968..2444)
                     /locus_tag="WHU08_RS28845"
                     /old_locus_tag="WHU08_28845"
     CDS             complement(1968..2444)
                     /locus_tag="WHU08_RS28845"
                     /old_locus_tag="WHU08_28845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_108943684.1"
                     /translation="MISRKSSLTGYIQSKKVSLNMTFSITEVRERRIGSLVITKELYDK
                     LEKYSRSNGLITSTYNHGHGVVRYYTVKYCLQMNYHGQNHPQKYLIKRGGRYPSKYAMV
                     KKTVEELEYSTPTFDAICRNIAIKYPHMVKFANYISETPLKGFAATFNNSVGND"
                     /name="hypothetical protein"
     gene            complement(2624..2764)
                     /locus_tag="WHU08_RS28850"
                     /old_locus_tag="WHU08_28850"
     CDS             complement(2624..2764)
                     /locus_tag="WHU08_RS28850"
                     /old_locus_tag="WHU08_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042863642.1"
                     /translation="MDCKHEPPVQSDRCALPGNYRLESNPERHDKTPLAAAIGRGCTRD
                     Y"
                     /name="hypothetical protein"
     Typing          3147..3325
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.946"
                     /coverage="83.0"
                     /evalue="3.5099999999999995e-46"
     Typing          complement(2429..2937)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.528"
                     /coverage="99.0"
                     /evalue="1.7000000000000002e-157"
     Typing          69..1573
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.821"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccctgatgg caatagacgc gggactggag cggctgcggc atgcggtgct ggaaaaaggg
       61 ggggatgatg atcgttaaat ttcatccccg ggggcgcggt ggtggtgccg gtccggtgga
      121 ttacctgctg ggaaaagacc ggcagcgcga gggggcgacc gtgctgcagg gaaaaccgga
      181 ggaagtccgg gagcttatcg atgcctcgcc gtatgcgaaa aagtacacct ccggcgtgtt
      241 gtcgttcgcg gaggctgaac tgccgcccgg gcagcgggag cagatcatgg ccagctttga
      301 gcgggttctg atgcccgggc ttgataaaga ccagtacagc attctgtggg tggagcacac
      361 cgacaagggg cggctggagc tgaatttcct gatccccaac actgaactgc tgaccgggaa
      421 acgcctgcag ccgtattacg atcgcgccga ccggccgcgc atcgatgcct ggcagaccgt
      481 ggtgaacggc agactggggt tgcatgatcc aaacgccccg gagaaccgac ggttactgtt
      541 cacgccgtcc gcactgccgg aaacgaagct ggaagccgct caggcgatta cacggggttt
      601 actggcctta gcctcatccg gggagctcaa aacgcgcgag gacgtcacag gggcgctcac
      661 agcggcaggt tttgaggtgg tccgcaccac gaaaaacagt atcagcattg ccgacccgga
      721 cggggggcga aacatccgac tgaagggagc catttatgag cagtctttta gcgctggcga
      781 aggacttgga gcagcaatcg aaagcgcagc agcagagtac cggcgagatg ctgaaagccg
      841 cattcagcga gcacgagcag tctgtaaaag cggaactgag cgcaagcgcg aagagaatca
      901 gcgacgccat cagcgcccac gagcagggta tgaccgcggc gatgcagtcg aaccggctga
      961 gcgtgctgcg tatggtcggc cggacgtggc tgaccatcac cctggtctcg gtactgctga
     1021 tcgccacgag cgggagcatt ctgtggtggc aggggcagca gatcaccggg aattacctga
     1081 ctatccgggc gcaggagcgg acgcaggcca tgctgtcaga gaagaaccac ggcgtgcagc
     1141 tgtcgctctg cggggagcag aaactgtcct gcgtcaaagt gaacccgaaa gcgggagctt
     1201 acgggaagga agggaactgg atggtactgg agaggaaata gcccatgacg gaactggaaa
     1261 aacagttgct gaccgcatta gagcagctac atcaggacta ctcgcaaagg ctggacgagt
     1321 gggagagcgc cttcgcggaa tggcagaaga tgtctggtct tatgcaacgg gagaacgcgg
     1381 cgctgaacga gcgcgtcacg cgcttgagtc agcaggtggc gaacttgagc aggcagttac
     1441 agcgtttgag ccagtagtgc agcggcatga aatggcggtg gccgccgaac gggcatacga
     1501 gcagcgacag cacgaaaaag agctggcgga agcccgttcc cgtcagcgga gctataacgg
     1561 tccgacgctg ggctgaacag tatcatccgg tgacaggccg cacgaagaag ggctcttatt
     1621 tctcccgcta aataggttcg tgatcaaaaa gagcacggcc tatacctggg gggaattatg
     1681 aaactgctta acgtcattga agctgtactt aactgtctgt atctggccgc cgggctgtgg
     1741 gactggctgg agaggcattc gccggttttt aaggcattgg cgcggatttt agtgtgactt
     1801 taacatccat gcatctggct gcatgactat gcaccagtgt aatattcagc ggctgcgttt
     1861 ccggtgtttt ttcgttatgt ctgtcgccac tttctcgcgt ctggcaccgg aaacgcagcc
     1921 aaccccgttt cagtggtgcg ggcaagggat gttatggtaa ataacactta atcattacca
     1981 acggagttat taaaggttgc ggcgaagcct tttaatgggg tttctgatat gtaattagca
     2041 aacttgacca tgtgtgggta ttttattgct atgttcctac atattgcgtc aaatgtcggt
     2101 gttgaatact ctaactcttc aacggttttt ttgaccattg catacttgct gggatatctc
     2161 ccccctcttt taataagata tttttgagga tgattttgtc cgtgatagtt catctggagg
     2221 caatatttca cggtgtagta tctaacaacg ccatgaccat ggttgtatgt ggatgttatt
     2281 aatccatttg agcgagagta tttttctaat ttatcgtaaa gctcttttgt aataactaaa
     2341 gaaccgatac gcctctcacg cacttctgtt attgaaaaag tcatatttaa gctcactttt
     2401 ttactctgaa tataacctgt tagagaggat tttcttgaga tcatccgtct gtcgtcgtag
     2461 tctcttatcc agaaacgaaa aaaccacctg gggaggtggt ttttcgaagg ttcagtaagt
     2521 tggggaactt ctgaaccgtg gtaacagggt gtacaagacc gctgccacca aattcgtcct
     2581 tccagtttag ccgtagtttg gctctaactt caagaactct gcatcagtaa tctcttgtac
     2641 accctctacc aatggctgct gccagtggcg ttttgtcgtg tctttccggg ttggactcaa
     2701 gacgatagtt accgggtaag gcgcagcggt cggactgaac ggggggttcg tgcttacagt
     2761 ccagcttgga gcgaactgcc tacccggaac cgagtgtcag gcgtggaatg agacaaacgc
     2821 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     2881 gagccaccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     2941 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaagcgccgc
     3001 tggcgcggcc ttctccgacc ttctcttttt tcgcgcacat gttgtatggc gccttatccc
     3061 ctgactctgt ggatacatca tgagcccgtt gtgtgcgcgc aggcaacgcc cgccgcagcc
     3121 gaacggccgg agcgtcagcg accgagtgag cgaggaggcg tcatatctcc ggaacctcca
     3181 ttttctcccg tacgccccgc ggctgcattt cccctgccac atgaagcact ttcattaaat
     3241 gttactccgc cgtaacatat tgagccagta tacactccgc tagcgctacg tgactggttc
     3301 agggctgcgc cccgaaaccc gctaaaagcg actgacgcgc ctgcggcttg tccaacaccg
     3361 tgccgaccgg gaaacatttc ccgcccgtcc cggtgttatc aggaggctac tctggcagac
     3421 aaacgcaaca agatgctcac gatgtgggtg acggaagacg aacaccggcg gctgctcgag
     3481 cggtgcgacg gcagacagct ggcggcgtgg atgcgtcaga cctgcctcga tgagaagccg
     3541 gcgcgtgccg gcagactgcc gtcaatatcg ccggcgctgc tgcgccagct tgccggtatg
     3601 ggtaacaacc tgaaccagat cgcccggaaa gtgaatgccg gtggtgccgg tcatgatcgg
     3661 gtgcagattg tggcc
//
