LOCUS       NZ_CP148489             3373 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0004 plasmid pZ1323CEC0004-7,
            complete sequence.
ACCESSION   NZ_CP148489
VERSION     NZ_CP148489.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441571
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096905.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:33:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148489.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0004"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0004-7"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-23"
     gene            complement(442..573)
                     /locus_tag="WHU49_RS00005"
     CDS             complement(442..573)
                     /locus_tag="WHU49_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484592.1"
                     /translation="MGVERKQLARKASCFGFIEVSDLLNVPVETVEIHNRNQFIQFA"
                     /name="hypothetical protein"
     gene            complement(564..698)
                     /locus_tag="WHU49_RS00010"
     CDS             complement(564..698)
                     /locus_tag="WHU49_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484593.1"
                     /translation="MQHPENAFHAFLGIQPGENMVFLLFERKDVKNLVIALAIFRRWA"
                     /name="hypothetical protein"
     gene            complement(872..1105)
                     /locus_tag="WHU49_RS00015"
                     /old_locus_tag="WHU49_27875"
     CDS             complement(872..1105)
                     /locus_tag="WHU49_RS00015"
                     /old_locus_tag="WHU49_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1112..1672)
                     /locus_tag="WHU49_RS00020"
                     /old_locus_tag="WHU49_27880"
     CDS             complement(1112..1672)
                     /locus_tag="WHU49_RS00020"
                     /old_locus_tag="WHU49_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_227316847.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGGWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1704..2360)
                     /locus_tag="WHU49_RS00025"
                     /old_locus_tag="WHU49_27885"
                     /pseudo=""
     CDS             complement(<1704..2360)
                     /locus_tag="WHU49_RS00025"
                     /old_locus_tag="WHU49_27885"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2350..2673)
                     /locus_tag="WHU49_RS00030"
                     /old_locus_tag="WHU49_27890"
     CDS             complement(2350..2673)
                     /locus_tag="WHU49_RS00030"
                     /old_locus_tag="WHU49_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2737..2928
                     /locus_tag="WHU49_RS00035"
                     /old_locus_tag="WHU49_27895"
     CDS             2737..2928
                     /locus_tag="WHU49_RS00035"
                     /old_locus_tag="WHU49_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3010..3090)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(790..2360)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggtggaag gtcggaggga acggcaacaa ctcacccctc ccagtgaagg gacgggtagt
       61 ttgccgctct cgatatgccg gacggagcca aggccatcga tcacggggcg cgtcagtgga
      121 agaacccgcg cctgcgtgac gtatgcgtct tcgccgcacc atctcacccg tcacgccacc
      181 acataccggg aactgccgcc aaggtctaac tcaaccaatc tagccgcgct acatacggtt
      241 tttgtggcaa ctcatcaggt gtccgctgtc gatgaaactc tctgcgccgg tagcggaact
      301 ctcacgcgtg cggtcgccgt cgtcacagct cccccaaccg caaacgattc tcgcaaatac
      361 gcaagtatct gcaaaaacag gtttttttcg gggttcctgg gagactgtgc tgttgaaaaa
      421 ctcttgaaga atacagaagg ctcatgcaaa ctgaatgaac tggtttctgt tgtggatctc
      481 aacagtttcc acaggaacat tgaggaggtc agagacctca atgaacccga aacagctcgc
      541 cttcctggcg agctgttttc gttctacgcc caccgacgaa agatcgcaag tgcaataacg
      601 agatttttca cgtctttacg ctcaaaaagt aaaaaaacca tgttttcccc tggctgaatc
      661 cccaaaaacg cgtgaaatgc gttttcagga tgttgcatag ctatgcacaa atacggcaca
      721 cgatttgttt cgctcgcccc agccgtcgaa cgggtcaaaa tggcaagatt tcgtcacttc
      781 tccggtcaca tctccattga tggcccactc cgaacccgtg ccgcgcgttc ctgtacctca
      841 tgtctgtacc gggcacgctc ctgttcatcc cttagctgtt gttcgcgata aataaccgac
      901 tcaagcgatc cacttgcccg gctaatctct gcacctgctg actcaatatc acgacgcgct
      961 cgttcagtat cgcgttgttc cgttgcgtaa cctcgtgcat cttctgcaat tccgcgaagg
     1021 cgctctccca ctcgttcagc cgccgcatat agtcctgttg caaatgctct aaggcgttca
     1081 gcaaatgccc ttccagttcc gtcatactcg tttactccct gaccagcctc acggcgttct
     1141 tgtcgcccac cgtccaggtg ttttcccctt tcaccccgtc cggcaacacc agaaatttcc
     1201 cgttctcgcc attgacgaac gtcacccccc aggtttttgc ctcgagtttc gccagcgtgt
     1261 tttcctgctc tctgatagtc aggatatttt tcgcaatctt gctttcctgc cactccatca
     1321 gcccccagcc ccccaggaaa atcccgcccg tgacgctgag cgcggtcagc gccggaaaca
     1381 gtatccgccc tttgatcacc cgccagagca gttttttctg ctgccgggac agttcggcct
     1441 ctgtggtgct gaactgcatg ctcacggtgg tgctcagcgt atccagttgc tttttcacgg
     1501 atgttgtatg ttcgctgata gcgtctctga ttttctgcct gtttaagctc aatgccttgt
     1561 ctacagactc ttcgagcttc ctgaactcgc tgttcagcat gttctcggta gaagcggctc
     1621 gctctttcag tttcttctcg aagtctgttc ccatttgtaa aagattgctc atacagcgcc
     1681 ccctttagcc tgagattccg ccccccctcc gggtcggcaa tactgatgct gcttttggtt
     1741 gtcctcacca cctcaaaacc tgccgctgta agcgcctctg tgacatcctg acgcgttttt
     1801 attgctccgg catggtaaag agcctcaaga ccccgtgtaa tcgcttctgc ggcctcctgt
     1861 ttcgcttttg gcagattatt tggcgtgaca agcgtccgtc tgttctccgg tgcgttcggg
     1921 tcacgcagcc cgtaatgatg attaaccagc gtctgccagg catttatcct cggcctgtcc
     1981 gcacggtcgt agtacggctg gaggcgctta ccgctctgta gctccatgtt cgggatgaca
     2041 aaattcagtt cgagccgccc cttatcctgg tgttccaccc agagaatgct gtactggtct
     2101 ttttcgagac cgggcataag aacacgctca aagctcgtca tcactctttc gcgttcaccc
     2161 ggtggtagct ccttctccgc gaacgacaga acgcctgacg tgtatttctt cgaaaatggc
     2221 gtggaatcaa tgagttcccg cacttcttcc gggttacccc ggagcaccgt tgcgccttcg
     2281 cggttacgct cccgccccag caggtaatcg accgggccac ttccaccgcc tttccccctg
     2341 gcatgaaatt taactatcat cccgtacccc ctgttccctg acagcctgcc gcaactggcg
     2401 caattcaccc tcgatggcca tcagtgcggc caccacatga acccggtcaa tcgaagacca
     2461 ctggccgctg ttcaccttcc gggctgtctg gttcaggttg ttcccgatgg cagccagatg
     2521 ccgcagcaat ggcggtgcca gtgtcggcag ttttcctgtg cgtgcaaccg gctcccccag
     2581 acagacccgc cgcatccatt ccgccagtcg tttaccctcg cagcgttcca gtaaccgggc
     2641 gtgctcctca tcggtaaccc gtatcgtgag catcttctcg cgtttcatcg gtatcattcc
     2701 ccctaacaaa tcccccatac aaggagcctt aagaccatga ccaaacagga aaaaacagcc
     2761 cttaacatgg ctcgctttat cagaaaccag acgctaaccc tgctggaaaa actcaacgat
     2821 ctggacgcgg acgaacaggc agacatctgc gaatcacttc acgaccacgc cgacgaactc
     2881 taccgcagtt gcctcgcacg cttcggcgat gacggtgaaa gcaactgaaa catgcagctc
     2941 ccggagacgt tcacagcttg cctgtgagcg gatgccggga gcagacaagc ccgccagggc
     3001 gcgtcagtgc gggtgtcggg gcgcagccct gacccagttc acagagcgat agcgatgtgt
     3061 atactggctt tcctcatttt gcatcggtgc agagtggatg ggaagtgcac catgtcagga
     3121 gtaaaaagcc gcacagatgc gtaaaaacag cgcgcccgat aaacaacaca cacgcggaca
     3181 ctggcacagg cgcgtaagtg cacaatgctt gcctctgtga ctcttccgct tcctcgccca
     3241 ctcgctcact acgttcggtc gctggggctg cggcgagcgg tgggatcact ctcgttgtga
     3301 tgcagatccg tttttttttt tgcactggag ttatcaggag tagcggtgca catgggatcg
     3361 tgcgatatgc tgg
//
