LOCUS       NZ_CP148481             4750 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0005 plasmid pZ1323CEC0005_1-8,
            complete sequence.
ACCESSION   NZ_CP148481
VERSION     NZ_CP148481.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441572
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.9
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 110X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038096835.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 11:30:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 9
            CDSs (total)                 :: 9
            Genes (coding)               :: 9
            CDSs (with protein)          :: 9
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148481.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0005"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0005_1-8"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-24"
     gene            11..184
                     /locus_tag="WHU53_RS00005"
     CDS             11..184
                     /locus_tag="WHU53_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484607.1"
                     /translation="MLKEFNFLMNALSIVSNEKKELIEFIGHDRRFYERCTIEYVMGII
                     GRIKCRELKVAG"
                     /name="hypothetical protein"
     gene            complement(714..827)
                     /locus_tag="WHU53_RS00010"
                     /old_locus_tag="WHU53_29035"
     CDS             complement(714..827)
                     /locus_tag="WHU53_RS00010"
                     /old_locus_tag="WHU53_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071790583.1"
                     /translation="MHEPPVQSDYCALSGNYRLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            1013..1168
                     /locus_tag="WHU53_RS00015"
     CDS             1013..1168
                     /locus_tag="WHU53_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318241.1"
                     /translation="MSVDFCDARQGGGAYGKTPAVLASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(1296..1490)
                     /locus_tag="WHU53_RS00020"
                     /old_locus_tag="WHU53_29040"
     CDS             complement(1296..1490)
                     /locus_tag="WHU53_RS00020"
                     /old_locus_tag="WHU53_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041643.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1776..2099
                     /gene="mobA"
                     /locus_tag="WHU53_RS00025"
                     /old_locus_tag="WHU53_29045"
     CDS             1776..2099
                     /gene="mobA"
                     /locus_tag="WHU53_RS00025"
                     /old_locus_tag="WHU53_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2558..3310
                     /locus_tag="WHU53_RS00030"
                     /old_locus_tag="WHU53_29050"
     CDS             2558..3310
                     /locus_tag="WHU53_RS00030"
                     /old_locus_tag="WHU53_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249639.1"
                     /translation="MDTTSLISSISVPRLSFYETFLGCATDQEKVGAYVAYQDLSGDFF
                     CLVQMIEVALRNAINNTIKANHGPAWYDSIPQTKKSQDLVLNAKQKALDECGVRYTDDD
                     VICRLTFGFWVYMLDAPYRDTQRPCYIWTPENKAKTFPHAKNPLGGGDMKVKALFDEFH
                     KVLLFRNRLFHHEPIWKKHHCKSHSQAINNMLKEFNFLMNALSIVSNEKKELIEFIGHD
                     RRFYERCTIEYVMGIIGRIKCRELKVAG"
                     /name="hypothetical protein"
     gene            complement(3840..3953)
                     /locus_tag="WHU53_RS00035"
                     /old_locus_tag="WHU53_29060"
     CDS             complement(3840..3953)
                     /locus_tag="WHU53_RS00035"
                     /old_locus_tag="WHU53_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071790583.1"
                     /translation="MHEPPVQSDYCALSGNYRLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            4139..4294
                     /locus_tag="WHU53_RS00040"
     CDS             4139..4294
                     /locus_tag="WHU53_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318241.1"
                     /translation="MSVDFCDARQGGGAYGKTPAVLASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(4422..4616)
                     /locus_tag="WHU53_RS00045"
                     /old_locus_tag="WHU53_29065"
     CDS             complement(4422..4616)
                     /locus_tag="WHU53_RS00045"
                     /old_locus_tag="WHU53_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041643.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1526..1767
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.9300000000000005e-124"
     Typing          4652..4750
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.85e-44"
     Typing          complement(756..1267)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.821"
                     /coverage="97.0"
                     /evalue="1.7899999999999998e-152"
     Typing          complement(3882..4393)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.821"
                     /coverage="97.0"
                     /evalue="1.7899999999999998e-152"
     Typing          1777..2096
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.36e-62"
ORIGIN
        1 cataaataat atgttgaaag agttcaattt tttaatgaat gccttgagta tcgtctctaa
       61 cgagaaaaag gaacttattg agtttattgg gcacgatagg cgcttttatg aacgctgcac
      121 gatagagtat gttatgggga ttatagggag gattaagtgc agggagctta aggtagccgg
      181 ttgactttta aggaaaaatg tgatacaatc aaaagctctt accggcttta agtcgcgcag
      241 ccgaaaggct acgtaactct gttactgcgc gacttaaagc cggtaagagc gaaagagttg
      301 cgcaactaat tgatttcaaa gcttttttca caaaaagaac tttagtattc attttcataa
      361 tcctctattt cttatgccgt tctaatggca gcgttgactt tttattccat ctatttgaat
      421 ggttaattat accatatcta acatcaaatc ttaagggtaa atagctgatt cacatcagtt
      481 tttccattaa ttcacgaagt tttataattt aattacggat ttccttgagt tcatatcgcg
      541 cccatcgtaa tcttaaaaac gaaaaaacca cccggcaagg tggtttttcg aaggttcagc
      601 aaccggcaag tttctgaacc gcggtaactg gcttaagagg agcgcagtca ccaaaacttg
      661 ttattccagt ttagccttaa ctggcacgta acttcaagac taactcctct aaatcagtta
      721 ccagtggctg ctgccaatgg cgataagtcg tgcctttccg ggttggactc aagacgatag
      781 ttaccggata aggcgcagta gtcggactga acggggggtt cgtgcataca gtccagcttg
      841 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa
      901 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
      961 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     1021 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcctg cggtgctggc
     1081 ttcttccggt gctttgcttt ttgctcacat gttctttccg gctttatccc ctgattctgt
     1141 ggataaccgt attaccgcct ttgagtgagc tgacaccgct cgccgcagtc gaacgaccga
     1201 gcgtagcgag tcagtgagcg aggaagcgga agagcgcctt atgtgacatt ttctccttac
     1261 gctctgttgt gccgttcggc atcctgccct gagcgttata tctctgtgct attttctact
     1321 tcaaagcgtg tctgtatgct gttctggagt tcttccgcga gttcgtgcag cttctcacac
     1381 atggcggcct gttcgtcggc atcgagtgcg tccagctttt cgagcagtgt caggctctgg
     1441 ctttttatga agcccgccat gttgagtaca gtttgctgct gcttgttcat ctttctgttt
     1501 tctccgttct gtctgtcatc tgcgttatgt gattatatcg cgtaccactt ttcgacagtt
     1561 ttgccgccgg tattctgccg tctgactctt tgacgggcat ttctgcctga caatactgtc
     1621 actgccaaaa aaactgccgt gcctttgtcg gtaattcgaa cttgctgaca ggacaggatg
     1681 tgtaattgtt ataccgcgca tacatgcacg ctattacaat taccctggtc agggcttcgc
     1741 cccgacaccc catgtcagat acggagccat gttttatgac aaaacgaagt ggaagtaata
     1801 cgcgcaggcg ggctatcagt cgccctgttc gtctgacggc agaagaagac caggaaatca
     1861 gaaaaagggc tgctgaatgc ggcaagaccg tttccggttt tttacgggcg gcagctctcg
     1921 gtaagaaagt taactcactg actgatgatc gggtactgaa agaagttatg agactggggg
     1981 cgttacagaa aaaactcttt atcgacggca agcgtgtcgg ggacagggag tatgcggagg
     2041 tgctgatcgc tattacggag tatcaccgtg ccctgttatc caggcttatg gcagattagc
     2101 ttcccggaga gaaactgtcg aaaacagacg gtatgaacgc cgtaagcccc caaaccgatc
     2161 gccattcact tttatgcata gctatgcagt gagctgaaaa cgatcctgac gcatttttcc
     2221 agttttcact gggtaaaaca tctctttttg cggtgtttgc ggctgaatcg cgttcagcgc
     2281 gttttagcgg tgcgcgtaag gggagttatg gtaaattggc agcaaagtat tttctgatgt
     2341 tatggattgt gaagagtatt caatccttat tatgaaacaa taactataac tggttgtttc
     2401 atatagataa atataacaaa aaagcccact acagagtggg cttttttgtt atattttacg
     2461 ctctagaggt acacagaaaa acggtagttc gcctgctcct cgaatgttgt aatggtagtc
     2521 tgaaaaatat tcatagtaaa gagataagtg agttttcatg gatacaacaa gtttaattag
     2581 ctcaatttct gtaccaagat tatctttcta tgaaacattt cttgggtgcg ctactgatca
     2641 agaaaaggtt ggggcatacg ttgcctacca agatctgtct ggcgattttt tttgtcttgt
     2701 ccagatgatt gaggttgctc tgcgtaatgc gataaacaat acaatcaaag ctaatcatgg
     2761 gcctgcttgg tacgactcta tacctcaaac aaagaaatct caggatcttg ttttaaatgc
     2821 gaaacaaaaa gcactagatg agtgtggcgt aaggtatacg gatgatgatg ttatttgccg
     2881 tctaaccttt ggcttttggg tttatatgtt agatgcccca tatagggata cgcaaaggcc
     2941 gtgctacatc tggactccag agaacaaggc aaaaacattt cctcatgcga agaatccttt
     3001 gggtgggggg gatatgaagg ttaaagcgct gtttgacgag tttcataaag ttcttttgtt
     3061 taggaatcga ctttttcacc atgagcctat ttggaaaaaa caccactgca agtcgcatag
     3121 tcaagccata aataatatgt tgaaagagtt caatttttta atgaatgcct tgagtatcgt
     3181 ctctaacgag aaaaaggaac ttattgagtt tattgggcac gataggcgct tttatgaacg
     3241 ctgcacgata gagtatgtta tggggattat agggaggatt aagtgcaggg agcttaaggt
     3301 agccggttga cttttaagga aaaatgtgat acaatcaaaa gctcttaccg gctttaagtc
     3361 gcgcagccga aaggctacgt aactctgtta ctgcgcgact taaagccggt aagagcgaaa
     3421 gagttgcgca actaattgat ttcaaagctt ttttcacaaa aagaacttta gtattcattt
     3481 tcataatcct ctatttctta tgccgttcta atggcagcgt tgacttttta ttccatctat
     3541 ttgaatggtt aattatacca tatctaacat caaatcttaa gggtaaatag ctgattcaca
     3601 tcagtttttc cattaattca cgaagtttta taatttaatt acggatttcc ttgagttcat
     3661 atcgcgccca tcgtaatctt aaaaacgaaa aaaccacccg gcaaggtggt ttttcgaagg
     3721 ttcagcaacc ggcaagtttc tgaaccgcgg taactggctt aagaggagcg cagtcaccaa
     3781 aacttgttat tccagtttag ccttaactgg cacgtaactt caagactaac tcctctaaat
     3841 cagttaccag tggctgctgc caatggcgat aagtcgtgcc tttccgggtt ggactcaaga
     3901 cgatagttac cggataaggc gcagtagtcg gactgaacgg ggggttcgtg catacagtcc
     3961 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg
     4021 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     4081 gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg ggtttcgcca cctctgactt
     4141 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgcctgcggt
     4201 gctggcttct tccggtgctt tgctttttgc tcacatgttc tttccggctt tatcccctga
     4261 ttctgtggat aaccgtatta ccgcctttga gtgagctgac accgctcgcc gcagtcgaac
     4321 gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgccttatgt gacattttct
     4381 ccttacgctc tgttgtgccg ttcggcatcc tgccctgagc gttatatctc tgtgctattt
     4441 tctacttcaa agcgtgtctg tatgctgttc tggagttctt ccgcgagttc gtgcagcttc
     4501 tcacacatgg cggcctgttc gtcggcatcg agtgcgtcca gcttttcgag cagtgtcagg
     4561 ctctggcttt ttatgaagcc cgccatgttg agtacagttt gctgctgctt gttcatcttt
     4621 ctgttttctc cgttctgtct gtcatctgcg ttatgtgatt atatcgcgta ccacttttcg
     4681 acagttttgc cgccggtatt ctgccgtctg actctttgac gggcatttct gcctgacaat
     4741 actgtcactg
//
