LOCUS       NZ_CP148480             8213 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0005 plasmid pZ1323CEC0005_1-7,
            complete sequence.
ACCESSION   NZ_CP148480
VERSION     NZ_CP148480.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441572
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8213)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096825.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:29:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148480.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0005"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0005_1-7"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-24"
     gene            11..163
                     /locus_tag="WHU53_RS00005"
     CDS             11..163
                     /locus_tag="WHU53_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341484599.1"
                     /translation="MELSRKWNNVSYTDSEKEEIQQSILKQIEKYSDFPYVVNYYRNRL
                     SALFD"
                     /name="hypothetical protein"
     gene            complement(355..516)
                     /locus_tag="WHU53_RS00010"
                     /old_locus_tag="WHU53_28980"
     CDS             complement(355..516)
                     /locus_tag="WHU53_RS00010"
                     /old_locus_tag="WHU53_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(1186..1377)
                     /locus_tag="WHU53_RS00015"
                     /old_locus_tag="WHU53_28985"
     CDS             complement(1186..1377)
                     /locus_tag="WHU53_RS00015"
                     /old_locus_tag="WHU53_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1441..1764
                     /locus_tag="WHU53_RS00020"
                     /old_locus_tag="WHU53_28990"
     CDS             1441..1764
                     /locus_tag="WHU53_RS00020"
                     /old_locus_tag="WHU53_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1973..>2404
                     /locus_tag="WHU53_RS00025"
                     /old_locus_tag="WHU53_28995"
                     /pseudo=""
     CDS             1973..>2404
                     /locus_tag="WHU53_RS00025"
                     /old_locus_tag="WHU53_28995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2442..2960
                     /locus_tag="WHU53_RS00030"
                     /old_locus_tag="WHU53_29000"
     CDS             2442..2960
                     /locus_tag="WHU53_RS00030"
                     /old_locus_tag="WHU53_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2967..3200
                     /locus_tag="WHU53_RS00035"
                     /old_locus_tag="WHU53_29005"
     CDS             2967..3200
                     /locus_tag="WHU53_RS00035"
                     /old_locus_tag="WHU53_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3566..4705)
                     /gene="dcm"
                     /locus_tag="WHU53_RS00040"
                     /old_locus_tag="WHU53_29010"
     CDS             complement(3566..4705)
                     /gene="dcm"
                     /locus_tag="WHU53_RS00040"
                     /old_locus_tag="WHU53_29010"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_033550328.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAIMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            4776..5681
                     /locus_tag="WHU53_RS00045"
                     /old_locus_tag="WHU53_29015"
     CDS             4776..5681
                     /locus_tag="WHU53_RS00045"
                     /old_locus_tag="WHU53_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_338933686.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAAYSALFDHGLASK
                     SDQRFASIRSTGNLTFSTAF"
                     /name="type II restriction endonuclease"
     gene            complement(5678..6433)
                     /gene="istB"
                     /locus_tag="WHU53_RS00050"
                     /old_locus_tag="WHU53_29020"
     CDS             complement(5678..6433)
                     /gene="istB"
                     /locus_tag="WHU53_RS00050"
                     /old_locus_tag="WHU53_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="WP_001282653.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLVD
                     DELLSRENSRVARLRKNAGLKYQATPEGLRYPASRGLRAEQMRELLNGHYITHRKNLLI
                     TGPTGCGKSWIANALGEQACRQKHSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQKIQV
                     LILDDLGLEQLSNAQCNDLLELIEDRYGQSSTIVVSQFPVDKWHGLMENPTTADAILDR
                     LVHNSHRVVLQGESLRKNPPVVESSEKTR"
                     /name="istB"
     gene            complement(6450..7985)
                     /gene="istA"
                     /locus_tag="WHU53_RS00055"
                     /old_locus_tag="WHU53_29025"
     CDS             complement(6450..7985)
                     /gene="istA"
                     /locus_tag="WHU53_RS00055"
                     /old_locus_tag="WHU53_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_023181050.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSLS
                     WPLPADISHDTLEKLIFPPKDTFASELVMPDMLYLDTEMRKPGVTRQLLWMEYKAQAGN
                     KAMGYSHFCRCYREWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFV
                     AVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVI
                     NDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRILDAPALSPLPLAPYEYTEYKAVKVGPDYHVEYA
                     RHWYSVPHELVGQRLSLKAGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHRQH
                     GTWTPERLIEQGKRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYGPERLEKAC
                     YVALHYNAPDRRFIDNLLRHHRENLELPLSRQGEQHPAYASEHENLRGPGYYH"
                     /name="istA"
     Typing          1014..1101
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(445..990)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.353"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1755..3304
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcagtcagca atggaattat caagaaagtg gaataatgta agttacacag actctgaaaa
       61 agaggaaatt caacagtcaa tattaaaaca aatagaaaaa tatagtgatt tcccttatgt
      121 tgttaattat tatagaaata ggcttagtgc tctttttgac taaaaaacta aaaggatctc
      181 ttgagatcct ttttttctgc gcgtaatctt ggacctgtaa acgaaaaaac caccctggca
      241 ggtggttttt tcgaaggtta gctaatcctg gcagattatc taaccgaggt aatctggctt
      301 cagcagagca cagataccaa atactgtcct tccagtgtag ccgtagttag gccatcactt
      361 caagaactct gtaagcatct ggataaatct cgctctgcta atccggttac cagtggctgc
      421 tgccagtggc gttaaggcgt gtcttactgg gttggactca agacgatagt taccggataa
      481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      541 ctacaccggg ccgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg
      781 cgcggccttt tgttgccctt tgccttttta ctcacatgtt ctttccggcc ttatcccctg
      841 attctgtgga taaccgtatt tccgcctttg agtgagccgg caccgcttgc cgcagccgaa
      901 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtatgttc
      961 tccttacgca tctgtgcggc atttcacacc cggcatggcg tgcttttcat acaatccgca
     1021 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1081 tgcgccccga cacccgctaa aacctgctga cgcgccctga cgggcttgtc tgctcccggc
     1141 atccgctcac agacaagctg tgaccgtctc tgggagctgc atgtgtcaga ggttttcacc
     1201 gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca tcggcgtggt cgtgaagcga
     1261 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttttcca gcagcgttaa
     1321 tgtctggctt ctgataaagc gcgccatgtt aagggctgtt ttttcctgtt tagtcactga
     1381 tgcctccgtg taagggggat ttctgttcat ggggtaataa taccgatgaa acgcgagagg
     1441 atgctcacga tacgggttac tgatgatgaa catgccaggt tacttgaacg ctgtgagggt
     1501 aaacaactgg cggtatggat gcggcgggtc tgcctggggg agccggttgc ccgttccggg
     1561 aaactgccga cactggcacc gccgttactg cgtcagctgg ccgccatcgg aaataacctg
     1621 aatcagacag cccgtaaggt gaacagcggg cagtggtctt ccggtgaccg ggttcaggtg
     1681 gtggccgcac tgatggccat cggggatgag ctgcgccggc tgcgtctggc cgtcagggaa
     1741 cagggggcgc gggatgatag ttaaatttca tgccagggga aaaggtggtg gcagtggtcc
     1801 ggttgattac ctgctgggga gggagcgtaa ccgcgaaggc gcaacggtgc ttcagggtaa
     1861 tccggaagaa gtccgggaac tcatcgatgc cacgccattt gcgaagaaat acacgtccgg
     1921 tgttctgtcg ttcgcggaga aggagctgcc gccgggagga cgtgaaaaag tgatggcgag
     1981 ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag tacagcattc tgtgggtgga
     2041 gcaccaggac aagggacggc ttgagctgaa ttttgtcatt ccgaacatgg agctacagac
     2101 cggaaaacgt ctccagccgt actacgaccg cgcagacagg ccgagaattg atgcctggca
     2161 gacgctggtg aatcaccatt acggactgca tgaccccaac gccccggaga accgccggac
     2221 gctgactctt cctgataacc tgccggaaac gaaacaggca cttgctgaga gcgttacacg
     2281 cggtatagac gcactttacc atgccggaga gataaaaggc cgtcaggatg tgattcaggc
     2341 gctcacagag gcggggcttg aagtggtcag ggtgacgcga agcagtatca gcattgcaga
     2401 cccgaacggc gggaggaata tcagactgaa aggagcgttt tatgagcaat cttttacaga
     2461 cgggcgcgga gttcgagaaa aagctgaaag agagagccga atctacagag aaaatgctga
     2521 acgacgagtt caggaagctc ggcgagtctg tcagcgaggc tgtgacctca aacgagacga
     2581 aaatcagaga cgctatagcc ctgttcacag cctcgacaga gaaatcgctg gaaaaacacc
     2641 gggaaggggt gaaagaggcg atgatgcagc acaggaagga cgtgttaaag ctggcaggga
     2701 atacgggcat gatgttactg gggatagtct ttctcctgtt taccgcgagt ggcgggacgc
     2761 tctggtatct tggcggacgg atacagacga acctggaaga aatcaggaag caggaagaga
     2821 cattgcagaa actgaacgcg aagacatggg gcgtggagtt tgtgcaggac gggaacagga
     2881 aattccttgt ccttccgtac gggaaatcag cggaggtgat tccctttcag gggaaagagt
     2941 gggtacatct gaaggagtga cacagagtga cagagctgga aatacatttg ctgaacgcct
     3001 tagagcagct gcaacaggac tatatgcagc ggctgagcga atgggagagc gccttcgtgg
     3061 aattgcagaa gatgttttcg cttacgcaac gggacaacgc gatgctgaac gagcgggtca
     3121 tgcagttgag tcagcaggtg cagcacttga gcgagcagac agaacgcttg agccagttat
     3181 acagcgagaa ctggagataa gagaggaacg gctgatacag gagcgcgaac atgcgttatc
     3241 cctggaacgg gagcgtcagc cggaaataca ggaacgcacg ctggatggtc cttcgctggg
     3301 atggtgaaac catgaaaaat ggcagcttca gtggattaag aggggttaat gtggcctgta
     3361 ccatctggtt gcataggtat tcatgcggtt aaaatttatc aggcgcgatc gcggcagttt
     3421 ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttc
     3481 agcggtgcgc gtaatgcgac gttatggtaa atccatatgg gggggtaatt ggattctggg
     3541 ccgcattgtg tccttatgac ttagtttatg aatctaaggt cgatatgatt ttttctgcta
     3601 ttgcattgat aacagggact gcgactgaat tcccaaattg cttataagcc tgtgtatccg
     3661 atacagggat aataaaatca ctaggaaacc cttgaagtct tgacgcttcc cgcggagtta
     3721 tttttcttgg gttacttcct ttttgctcta ttaatatttc actaccatct ttatagtacc
     3781 tcgcacttat ggtattagta tatgggctat tttcattaaa taaaccatac ccaaagcctt
     3841 taccggctgc tgcatttaca agtttcctgc gttggtgtcc attccagagc gcatctgata
     3901 gcgtatattt attgtctacg ctcttttcaa gaatatctcc aactcttgtt tttgttttta
     3961 acggggttgg aaaagtaaaa tgttcatgat ttcttacttt ttctttatta aagcctacaa
     4021 tgtatatgcg ttctctattt tgtggaacac caaagtcttt tgcagcgaag atattgtaat
     4081 acacagtgta attcaattct tcaagtgtgt tttttattat tgaaaaagtt ctacctttat
     4141 catgtcctag caagtttttt acattttcaa gtaaaaaagc atgaggtttt ttttccttta
     4201 ttatccttgc tatatcaaaa aacaaagttc ctcttgtatc attaaatcct tttttgagcc
     4261 ctgcttgaga gaatgctacg caaggaaacc caccgacaag aatttcatga tcaggaatat
     4321 ctttttcatc gatttttgtt atgtccccat cagggaaatc accataattt gcatgatatg
     4381 tcttttgagc aaacttatcc cattcgctgg aaaaaacaac atttacagcg ttagtttgat
     4441 gaaatccaag tctggttcca ccgattccag caaataaatc gatcatgcga tatctgccat
     4501 tctcgttgtt tgggtaaggt ggtgtgtctg gaaagtccat aattgctttt agttccgccc
     4561 cagtaggttt tgtttccccg cgctcccatc ttcttatggt tctgtctcca tattttgaaa
     4621 gaccaagggc gtctgcaaac tctttttgag tcatatgaag tctttctctt tttcctttga
     4681 ttgttgctgc gatattatca gtcatgcatg tctaccagaa ttaagtgttg gttttaggac
     4741 aatttgtcct gttttgattc caattatgaa cgtgcatgaa aaaactttca agtagatata
     4801 aaaggttatt ttttatgcaa agattgagtc ctggtgaatt taaaactcta atttctaaag
     4861 agagaaagtc gcattttatt acgccatttg ctttggttta taaaacattt tgtgatttag
     4921 ggtatgatca aaaaaatagt gattattttt tgaataatcc aagtgaatat ataattgcaa
     4981 tgagaaaaaa ctgttggaaa gaatttgaac catttgaaaa agaattcaca acaagaatgt
     5041 tatcatatct tattgatgaa gaaagaataa aagatatgag tccttatgat gccattcgtg
     5101 attttacgat ggaataccct acacacatat atgatttggc actgtctaac actcaatcca
     5161 gacgtagcag ggcaggaaaa gagtttgagt ctattcttga gctattaatg atgggggcag
     5221 gaattcctgt tgatgtgcag ggggctattg ggaagtcatt ttttcaaaaa aaccaaatag
     5281 gtaaacttgt agatcttgtt atgccaggtg tagtccagta cacatctaac aaaagaaata
     5341 caatgttgat ttccgctaaa acaacgctga gggagcgatg gcaagaagta cctgaagagg
     5401 ttaatagaac aggaattaga gaaatgtatc tagctacact agatgattct tttagcgagg
     5461 agacaataaa tatattgtac gaggctaatg tggtcgttgt aacgacgata gaaaataaaa
     5521 atttcaaata taaaaataat aatagggtgc taacctttga agatatgcta cagtcagctg
     5581 cgtattccgc tctattcgat cacggattgg catcgaagtc cgatcagcga ttcgcatcca
     5641 ttcgatcaac cgggaactta actttttcta ccgcttttta acgcgttttt tcgctgcttt
     5701 ccactaccgg gggatttttc ctcagtgact ctccctgaag gaccacccgg tgtgagttat
     5761 gtaccagtct gtccaggatg gcatctgctg ttgttgggtt ctccatcaga ccatgccatt
     5821 tatctaccgg gaactggctt accactatcg tgctgctttg tccgtaacga tcctctatga
     5881 gctccagaag gtcattacac tgggcgttac ttaactgttc cagccccaga tcgtccagga
     5941 ttagaacctg tattttttgc aactgtttca gatatttcag ccagctgcca tcaacacgtc
     6001 cctgagccag ttgttccagt aatcgcccgg ttctacagta ccggacgctg tgtttctgtc
     6061 gacatgcctg ttcgcccagc gcattggcta tccagctctt gccacatcct gtcggaccgg
     6121 ttatcagcag gtttttcctg tgggttatgt agtggccgtt cagcagttct ctcatctgtt
     6181 cagcccgcag acctcttgag gccggatagc gcagcccttc cggtgttgcc tggtatttca
     6241 ggccggcatt ttttctcaac cgtgccacac ggctgttttc cctgctcaga agctcgtcat
     6301 caaccagcag cgtcagcctt tcctcgaacg acagttcgtc gtaggtctga ggcgtttccc
     6361 gctgccgtat caggctcccg gccatcgccg ggagtttcag ttgggtcagt ttgtttaaca
     6421 ggttatcact catactgttg ttccttctgt cagtggtagt agcccggacc acgcaggttt
     6481 tcatgctctg aagcatatgc cggatgttgt tcgccctggc gggataatgg aagctccagg
     6541 ttctcccgat ggtgacgcag cagattgtcg ataaagcgtc tgtcaggggc gttatagtgc
     6601 agggcaacgt aacaggcctt ttccagccgt tccgggccgt attttttctg aagtgccagc
     6661 agccccagaa ctgcacgata agccagttcc ggatggggtt ttgccttcag catggattca
     6721 accacccggc ctgtggacgg gcccgtacgt tttccctgct cgatcaggcg ttccggtgtc
     6781 catgtcccgt gctgccggtg gcgctctggc atgtgaagcg gattggttgt gtgtttatat
     6841 tcatgtgtgc tgcgcggatg ttgcgccaca cactgcccct tgtgccagag ctgtaccacc
     6901 gactgccccg ccttcagtga cagacgctga ccgaccagtt cgtgtggaac ggagtaccag
     6961 tgacgggcat attcaacgtg gtagtcaggg ccaaccttca cagctttgta ttccgtgtat
     7021 tcataaggtg ccagaggcag cggagagagt gctggcgcat ccagtatgcg gaagcgttct
     7081 gcacgggttt gattgccgta ccccttcatt ggccggttat tcatgtctgt cagtaactcc
     7141 ctaaggcgcg cattcagcgc cctgagcgtg tggaaggttt cattacggat gcgggcaaga
     7201 agccatcgtt cgacgatgag gacgccgttt tctgcttttg gtttatcttt aggtttgcgt
     7261 ggtcgggccg ggatgataac cgtgccatag tgctcagcca gtgcctgata actgtcgttg
     7321 atgacaggct catatcgatc ggcctttttc actgcgcttc tgaggttatc ggggatgagc
     7381 agcttcggta cgccgcccag gaaggtcagg caacggctgt gggcatttaa ccatgacatc
     7441 atgtcctgac cttcgcacgc ttcaacgtag gtatagttgg acgcccccat tacggccacg
     7501 aagattgcca cgcggcgtat ctcaccggtg tccggattaa taacaggaac cgtaggacca
     7561 cagaagtcga taaaaagctt ttcgccggca cgatgctcct ggcgcatgga gagacgtcgc
     7621 gtttttttcc actcccggta gcaacggcag aagtgcgagt atcccatcgc tttgttaccc
     7681 gcctgtgcct tgtattccat ccacaacagt tgccgcgtca cacctggttt tctcatttcc
     7741 gtatcgaggt acaacatgtc aggcattaca agctctgatg caaaggtgtc tttgggcgga
     7801 aaaatgagtt tctccagcgt gtcatgggat atatcagcag gtaatggcca tgacagagaa
     7861 gaggctttga agcgggcaag aacctcaaaa acggttgagg cgctgatgtg taacgtcagt
     7921 ccgatacaac ggttagtcag cttcttgtcg aacttgagct gaagaatctt aaaacaggtt
     7981 tccatagtgc tcctggattt tctctttctg gacattgacg tctccactgg tttaaacacg
     8041 gcaatggaga ctgtggtgaa aagagttaat tcccggagtg actggcagga tgccaatcaa
     8101 tgatcggaag catgccaaat cgtgatcgat tgtgatgcca aactgtgatc ggagatggat
     8161 gccaaatcat gatcgattcg aatgccaaac tgcgatcgat tcagatgcca ttt
//
