LOCUS       NZ_CP148479             8561 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0005 plasmid pZ1323CEC0005_1-6,
            complete sequence.
ACCESSION   NZ_CP148479
VERSION     NZ_CP148479.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441572
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096815.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:32:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 17
            CDSs (total)                      :: 17
            Genes (coding)                    :: 12
            CDSs (with protein)               :: 12
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 4 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148479.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0005"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0005_1-6"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-24"
     gene            <2..238
                     /locus_tag="WHU53_RS00005"
                     /old_locus_tag="WHU53_28895"
                     /pseudo=""
     CDS             <2..238
                     /locus_tag="WHU53_RS00005"
                     /old_locus_tag="WHU53_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /name="hydrolase"
     gene            285..848
                     /locus_tag="WHU53_RS00010"
                     /old_locus_tag="WHU53_28900"
     CDS             285..848
                     /locus_tag="WHU53_RS00010"
                     /old_locus_tag="WHU53_28900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]; GO:1904047 - S-adenosyl-L-methionine
                     binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_010380838.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVTL
                     AEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRDNFNGVRVQCCDF
                     MEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLPFSD
                     VREELPRGTFAYTSVPTMIIRLRA"
                     /name="class I SAM-dependent methyltransferase"
     gene            complement(934..1359)
                     /locus_tag="WHU53_RS00015"
                     /old_locus_tag="WHU53_28905"
                     /pseudo=""
     CDS             complement(934..1359)
                     /locus_tag="WHU53_RS00015"
                     /old_locus_tag="WHU53_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1429..1635
                     /locus_tag="WHU53_RS00020"
                     /old_locus_tag="WHU53_28910"
     CDS             1429..1635
                     /locus_tag="WHU53_RS00020"
                     /old_locus_tag="WHU53_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000547945.1"
                     /translation="MHIPQKFLPKSRQKLLWSAPGQSFPAVAVMPLAAKVLKRVMVNTG
                     GNKRQVPAKRQNRGSRNSTGDGG"
                     /name="hypothetical protein"
     gene            1661..2209
                     /locus_tag="WHU53_RS00025"
                     /old_locus_tag="WHU53_28915"
     CDS             1661..2209
                     /locus_tag="WHU53_RS00025"
                     /old_locus_tag="WHU53_28915"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_069723129.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWHDKQ
                     TGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNNGVTRYITEILV
                     KNTGTMQMLGRAAGAQTQPEETQQFSGQPQQEPQPEPQVEAGTKKGSAKTKGRGRKAAQ
                     PEPQPPSEPEYNFDDDIPF"
                     /name="single-stranded DNA-binding protein"
     gene            2267..2500
                     /locus_tag="WHU53_RS00030"
                     /old_locus_tag="WHU53_28920"
     CDS             2267..2500
                     /locus_tag="WHU53_RS00030"
                     /old_locus_tag="WHU53_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="WP_000006001.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLVV
                     RNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
                     /name="DUF905 family protein"
     gene            2564..4522
                     /locus_tag="WHU53_RS00035"
                     /old_locus_tag="WHU53_28925"
     CDS             2564..4522
                     /locus_tag="WHU53_RS00035"
                     /old_locus_tag="WHU53_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="WP_338930351.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLAQTEEVSVSLASLIK
                     SPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLAER
                     GILTADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTGDLL
                     GYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQSGWG
                     GKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDRVALDAA
                     LLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYCSLPDPEAVLTEAEEERLNEL
                     MTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRSGSGVVVSWCYGNVCVQRGVQ
                     LRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALLAW
                     TLCLNVFGSGAYSSPVRIYLECGHYSLTSAAPSGKEGTAFMALMAEHFRLAALLPDEWE
                     RDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAIGFHMRDWWQP
                     TKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPGWMCAP
                     HPQTDTTERTGNLADAA"
                     /name="ParB/RepB/Spo0J family partition protein"
     gene            4577..5011
                     /gene="psiB"
                     /locus_tag="WHU53_RS00040"
                     /old_locus_tag="WHU53_28930"
     CDS             4577..5011
                     /gene="psiB"
                     /locus_tag="WHU53_RS00040"
                     /old_locus_tag="WHU53_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="WP_000845950.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPDN
                     WTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVVQV
                     QRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQV"
                     /name="psiB"
     gene            5008..5727
                     /locus_tag="WHU53_RS00045"
                     /old_locus_tag="WHU53_28935"
     CDS             5008..5727
                     /locus_tag="WHU53_RS00045"
                     /old_locus_tag="WHU53_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="WP_069723131.1"
                     /translation="MSVRSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAFF
                     RCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAVDVLIETQGEVCLLPLPGDAAER
                     LFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPETVG
                     SWHARWSDRVTEHDLETLFWQWGERFPSLSGMERWQWQDMPFWQVIAEASMAAKEAGHA
                     VREMERWMVPNKLREAA"
                     /name="plasmid SOS inhibition protein A"
     gene            5727..6245
                     /locus_tag="WHU53_RS00050"
                     /old_locus_tag="WHU53_28940"
     CDS             5727..6245
                     /locus_tag="WHU53_RS00050"
                     /old_locus_tag="WHU53_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_069723134.1"
                     /translation="MRNVRHPKTHRELCELACRFLRNNGFKVVFSDRFHAWTSCGEHPD
                     ALGFRNGASCLIEVKCSRSDFLADRKKVFRAEPEKGMGDWRFYLCEPGVITVDDLPAGW
                     GLLYAINGRVRKVHGWPGNHQWVVTVAKPFRANKQAECDYLFSALRRLEIRGVTENGAD
                     ADRVQIYGL"
                     /name="hypothetical protein"
     gene            6363..>6512
                     /gene="mok"
                     /locus_tag="WHU53_RS00055"
                     /old_locus_tag="WHU53_28945"
                     /pseudo=""
     CDS             6363..>6512
                     /gene="mok"
                     /locus_tag="WHU53_RS00055"
                     /old_locus_tag="WHU53_28945"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
                     /name="mok"
     gene            6454..6579
                     /locus_tag="WHU53_RS00060"
                     /old_locus_tag="WHU53_28950"
     CDS             6454..6579
                     /locus_tag="WHU53_RS00060"
                     /old_locus_tag="WHU53_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="WP_000909453.1"
                     /translation="MLIVCLTLLMFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
                     /name="type I toxin-antitoxin system Hok family toxin"
     gene            complement(<6882..7177)
                     /locus_tag="WHU53_RS00065"
                     /old_locus_tag="WHU53_28955"
                     /pseudo=""
     CDS             complement(<6882..7177)
                     /locus_tag="WHU53_RS00065"
                     /old_locus_tag="WHU53_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            7247..7519
                     /locus_tag="WHU53_RS00070"
                     /old_locus_tag="WHU53_28960"
     CDS             7247..7519
                     /locus_tag="WHU53_RS00070"
                     /old_locus_tag="WHU53_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_244567340.1"
                     /translation="MHIPQNFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKQVMVNTG
                     GNKRQVPAKRQNRGSRNSTDDGGKKQRCIKDGQKYVSSTYRWLYS"
                     /name="single-stranded DNA-binding protein"
     gene            7473..7796
                     /locus_tag="WHU53_RS00075"
                     /old_locus_tag="WHU53_28965"
     CDS             7473..7796
                     /locus_tag="WHU53_RS00075"
                     /old_locus_tag="WHU53_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001712315.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCGL
                     HYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAWEAKDALMAELA
                     LEY"
                     /name="hypothetical protein"
     gene            7855..8097
                     /locus_tag="WHU53_RS00080"
                     /old_locus_tag="WHU53_28970"
     CDS             7855..8097
                     /locus_tag="WHU53_RS00080"
                     /old_locus_tag="WHU53_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000540591.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEIRDSDGHLLLKVSAPEAG
                     WTHESLNLVQPQEVQEGNDAFDAYLNGIWIGSTEV"
                     /name="hypothetical protein"
     gene            complement(8297..>8557)
                     /locus_tag="WHU53_RS00085"
                     /old_locus_tag="WHU53_28975"
                     /pseudo=""
     CDS             complement(8297..>8557)
                     /locus_tag="WHU53_RS00085"
                     /old_locus_tag="WHU53_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
ORIGIN
        1 cgccgtcgcc agagagaccg cgaagcgtcc gccggtagtc aactatccgg gggaaggttt
       61 ccgggaaatg acaaaggcac agtgggccgc cctgccccgg gactgtaagg ccgtgcgcag
      121 tatggcggag acagaagatc acggggcata ccgttaccgt cgcacaatgg acaacaattt
      181 ccgtctggtg aatgtgtata tcaccgacat gaaaatcacg gaaatcccgc agaaataaga
      241 tttacccccc gggaaaatcc ccgggggatg acggagataa tgacatgcac agtcagttaa
      301 gagaacgtat ccggctgatg cgcgcaaggc tggataacgc cgcgccggtt gctgaaatcc
      361 gggctgaatc tcagcttttt gtgactcctg ccccggtctg cgatcgcctg gtgacgctgg
      421 cggaaatcag caaccgtgat cacattctgg aaccctctgc cggcaccgga gccatactgc
      481 gggcgattcg ggataccgcg ccgggggcca tgtgtgatgc ggtggagatt aacagcgggc
      541 tggtccggta tctgcgggac aattttaacg gtgtcagggt acagtgttgc gacttcatgg
      601 aatggcagcc ggtgcagtat tacagccgga ttatcatgaa tccgccgttc agtcacgggc
      661 aggatatccg gcatatcctg cgggcttttt ccctgttgcg tccggggggc gtgctggttg
      721 ccgtctgtct gaacgggccg cgccagcagg agaagctgtt accgttttct gacgtccgcg
      781 aggagctgcc acgcggcacg tttgcttata ccagtgttcc gacgatgatt attcgtctgc
      841 gtgcctgatg acgccgggca ggcgggagga taaatgcccg tctgcccgcg ccgggaaaag
      901 accgcgcgcc ggtggcgctg aaaggggccg cgcttacgcg ccgccccttt cattacgcgg
      961 atgttctgca gtgtgcttct gtcttttcat gcctgtcatt tgtctgttac tgctgatgtt
     1021 gttgttcctg ctactgctac tgttctgctt tttcctgttg ctctgattac tgtctgtttt
     1081 gttgttctcc tgactgtgcc ttttccgggc gtccccctgg cggggcgggc tgtcgtgaac
     1141 ggagccagcg aagctgtctc cgccctggcg ggcttccatc cctgacgcga accccctggc
     1201 ttgcagcgac cgttccggtc gcgccgcctc tgtcgcgccg tgacctgcgc tcccggcatc
     1261 ccggcaaaag gccgccggga tgtaaggggc aacgccgttt cgcgcacgcg ctcttcgtcg
     1321 ttgcggcggg tccctgcccg ttgttctgtc cggtgccatt cacctgttta agcccctgtc
     1381 gccgggggca aagacagcca caaccgtctc cgccagtcca ggggtgaaat gcacatccca
     1441 caaaagtttt tgcccaaaag ccggcaaaaa cttttgtggt ctgcccccgg acagtcgttt
     1501 ccggccgtgg cagtgatgcc ccttgcggcg aaggttctca aacgggtgat ggttaatacc
     1561 ggagggaaca aacggcaggt ccccgcaaaa cgtcaaaacc gcggctcccg aaactcaacg
     1621 ggtgatgggg gctgaaaaca tcaacagaag gagacacatt atggcagttc gtggcattaa
     1681 caaggtcatt cttgtcggtc gtctgggaaa agatccggaa gtccgttaca tccccaacgg
     1741 gggcgcagtg gcaaacctgc aggtggccac gtcagaaagc tggcatgaca aacagacggg
     1801 ggagatgcgg gagcagacgg aatggcaccg cgtggtgttg ttcggcaagc tcgcggaagt
     1861 ggctggtgaa tatctgcgca agggcgcgca ggtctacatc gaggggcaac tccgtacccg
     1921 tagctgggac gacaacaacg gcgtcacccg ttatatcact gaaatccttg ttaaaaacac
     1981 gggcaccatg cagatgctgg gacgtgccgc aggtgctcag actcagccgg aagagacaca
     2041 acagttcagc ggtcagcctc agcaggaacc acagccggaa cctcaggtgg aggccggtac
     2101 gaaaaaagga agcgcaaaaa cgaaaggccg tggacgtaag gccgcgcagc cggagcctca
     2161 gccgccgtct gaacccgagt ataacttcga tgacgacatt ccgttctgat cgggctgatc
     2221 gtgataaccg ccccgttcag tgcggggcat caccggagaa atgaggatga gcgaatattt
     2281 cagaatactt caggggctgc cagacggtcc ttttacccgc aaacaggccg aagccgttgc
     2341 cgcacagtac cggaacgtct ttatcgagga tgaccacggc gaacagtttc gcctggttgt
     2401 ccgtaataac ggtgcaatgg tctggcgtac ctggaatttt gaggacggtg ccgggtactg
     2461 gatgaaccat gtcattcgtg atttcggcat tcttaagtaa gagaggtgcc ggacgggaac
     2521 gcgtccggca gacatacaac catcccggtc aaaggagaaa tatatgtcag ttacagagtc
     2581 taagacaaaa acggagcgta aatccagccg taaacctgca aaaacgcagg aaacagtcct
     2641 gtctgccctg ctggcgcaga cggaggaagt gagcgtgtcg ctggcctcac tgattaagtc
     2701 accgttgaat gtgcgcacgg tgccgtattc tgcggagtcc gtcagcgaac tggcggactc
     2761 cattaaaggt gtcggcctgt tacagaatct ggttgttcat gccctgcctg gtgaccgtta
     2821 cggtgtcgcc gcaggtggtc gccgactggc tgcactcaac atgctggcag agcgtggcat
     2881 ccttacggct gactggcctg tccgcgtgaa ggttattccg caggaactgg cgactgccgc
     2941 atcaatgact gaaaacggtc atcgtcggga tatgcaccct gccgaacaga ttgccggatt
     3001 ccgcgcaatg gcgcaggaag gcaaaacacc tgcgcagact ggcgacctgc tgggctattc
     3061 accccgccac gtccagcgaa tgctgaaact ggcagacctt gcacctgtca tcctcgatgc
     3121 gctggcagaa gaccgtatca ccacagagca ctgtcaggcg ctggcactgg agaacgacac
     3181 cgcgcgtcag gtgcaggtgt ttgaagctgc ctgccagtca ggatggggcg gtaaaccgga
     3241 tgtgcgggtt atccgcaatc tgattacgga aagtgaagtg gcggtggcgg ggaacagcaa
     3301 attccgcttc gtgggggctg atgccttctc acctgacgaa ctgcgcaccg atttgttcag
     3361 cgacgacgag ggtggctatg tggaccgtgt ggcgctcgat gccgccctgc tggaaaaact
     3421 ccaggctgtc gctgaacacc ttcgggaagc cgaaggctgg gaatggtgcg ccggacgcat
     3481 ggagcctgtc ggtgagtgcc gtgaggatgc cggaacatac tgcagtctgc cggatccgga
     3541 agcggtgctg acggaggcag aagaagaacg cctgaacgaa ctgatgacgc gttacgacgc
     3601 gctggaaaat cagtgtgagg aatccgacct gctggaagca gaaatgaagc tgatgcgctg
     3661 catggcgaag gtcagagcgt ggacgccgga gatgcgttcc ggaagcggtg tggtggtgtc
     3721 ctggtgttat ggcaacgtat gtgtccagcg tggtgtgcag ttgcgcagtg aggatgacgt
     3781 ggctgacgac gctgaccgca cggaacaggt gcaggagaaa gcgtcagtgg aggaaatcag
     3841 tctgccactg ctgacgaaaa tgtcttccga acgcacgctg gcagtccagg cggcactcat
     3901 gcagcaaccg gacaaatctc tggcactgct ggcatggacg ctctgcctga atgtgtttgg
     3961 cagcggggcg tacagtagtc ccgtcagaat ttacctggaa tgtgggcatt actcactgac
     4021 cagcgctgcg ccgtcaggga aggaaggtac cgcattcatg gcgctgatgg cagagcactt
     4081 tcgccttgct gccctgctgc cggatgaatg ggaacgggac atgacgacgt tcctgtcact
     4141 cagccaggag gtgctgttat ccctgctcag tttctgcacc gcatgcagcc ttaacggtgt
     4201 ccagacccgt gagtgtggtc acacgtcacg cagtccgctt gactcgctgg aaaccgctat
     4261 cggattccac atgcgtgact ggtggcagcc gacaaaagca aacttcttcg gacacctgaa
     4321 aaagccgcag attatcgcag ccctgaatga ggcaggactg tctggtgccg cacgggacgc
     4381 ggagaagatg aagaaaggcg atgcggctga acatgcagag ttccacatga aagacaaccg
     4441 ctgggtgcct ggctggatgt gtgcaccgca tccacagacg gataccactg aacgcaccgg
     4501 taacctggct gatgccgcct gatgaacaac cacaccgccc cgccggagac ggggcggcag
     4561 caagggagat accgtgatga aaactgaact gaccctgaat gtattacaga ccatgagcgc
     4621 acaggaatat gaagacatcc gggctgccgg aagtgatgaa cgtcgtgaac tcacacatgc
     4681 cgtgatgcgg gagctggatg cgccggataa ctggacgatg aacggcgagt atggcagcga
     4741 gtttggcggc tttttccccg tccaggtccg tttcacgcct gcccacgaac gttttcacct
     4801 ggcgttatgt tcaccgggcg atgtctctca ggtctgggtg ctggttctgg tgaatgccgg
     4861 tggtgagccg tttgcggtgg ttcaggtgca gcggcggttt gcaccggaag ccgtcagcca
     4921 ttcactggca ctggcggcat cactggacgc gcaggggtac agcgttaacg acatcatcca
     4981 tatcctgatg gcggaaggag gtcaggtatg agcgtacgtt cacaggcact ggttcccctc
     5041 agcacagagc aacaggccgc atggcgggcg gtggcggaga cagaaaaacg ccgccatcag
     5101 ggtaacacgc tggcggaata tccgtatgcg ggggcgtttt tccgctgtct gaacggaagc
     5161 cgcaggattt cgctgtctga cctgcgattt ttcatgccat ccctgaccgc agaagaactg
     5221 cacggcagcc gtcttcagtg gctgtatgcc gtcgatgtac tgattgaaac acagggcgag
     5281 gtctgcctcc ttccccttcc cggtgatgca gcagagcggc tgttcccgtc ggtacggttc
     5341 cgcgtcaggg agcgcagtcg ccataaatcc gcgctggtca tgcagaagta cagccgtcag
     5401 caggcacggg aggcagagca gaaagcccgt gcgtaccagg cgctggtcgc ccaggctgaa
     5461 atcgaactgg cgttccattc accggaaaca gtcggaagct ggcatgcacg ctggagtgac
     5521 cgggtgacag agcatgacct ggaaaccctg ttctggcagt ggggtgagcg ttttccgtca
     5581 ctgtccggga tggaacgctg gcagtggcag gatatgccgt tctggcaggt tatcgcggaa
     5641 gccagtatgg cagcaaagga ggccggtcat gctgtccgtg agatggagcg gtggatggtg
     5701 ccgaacaaac tccgggaggc ggcatgatgc ggaatgtcag acacccgaaa acacacaggg
     5761 aactgtgtga gctggcctgc cgttttctcc gcaacaacgg ctttaaggtg gtgttcagtg
     5821 accgttttca cgcatggacg tcctgcggtg aacatcctga tgccctgggc ttccgtaacg
     5881 gagcgtcatg tctcattgag gtgaaatgct cacggtctga tttcctggct gaccggaaaa
     5941 aggtgttccg ggcagagccg gaaaagggca tgggagactg gcgtttttat ctctgcgagc
     6001 cgggggtgat taccgtggac gatttaccgg cgggctgggg gttgctgtat gcgattaacg
     6061 ggcgggtcag gaaagtgcat ggctggcccg gaaatcatca gtgggtagtt acggtcgcga
     6121 aaccgttcag ggcaaacaaa caggctgaat gcgattatct gttcagtgct ctgcgtcggc
     6181 tggaaatccg gggcgtgacg gagaacggtg cagatgctga cagggtgcag atatatgggc
     6241 tatgatgtgt ccggcgcttg aggctttctg cctcatgacg tgaaggtggt ttgttaccgt
     6301 gttgtgtggc agaaggacaa aagccccgta gttaattttt cgttaaccca cgaggcccct
     6361 gcatgcttag acaacatcag gatagcctct taccgcgctt tgcgcaagga gaagaaggcc
     6421 atgaaactgc cacgaagttc ccttgtctgg tgtgtgttga tcgtgtgtct cacactgttg
     6481 atgttcactt atctgacacg aaaatcgctg tgcgagattc gttacagaga cggatacagg
     6541 gaggtggcgg ctttcatggc ttacgaatcc ggtaagtagc aacctggagg cgggcgcagg
     6601 cccgcctttt cagggctgat gctggcctga ctgcactgaa gcgcctttat gaagggactg
     6661 ctggtttatc cgttttccgg cagtcccttt ttccttactg gcattgtgcg ggcatgaaca
     6721 accaggcagc caggaaggcc gtttcccttc gggaacgggg atgaacgcgc gcctgcggcg
     6781 cccgttcttc tcccccgcct tctctggtta tggccagtca gaaatcacat cagcttccct
     6841 gtgattattc tcttttccct cctgttctga ttactgctgc cctgattact gtctgttttg
     6901 ttgttctcct gactgtgctt tttccgggcg tccccctggc ggggcgggct gtcgtgaacg
     6961 gagccagcgg agctgtctcc gccctggcgg gcttccatcc ctgacgcgaa acccgggctt
     7021 gcagcgaccg gaacggtcgc gccacccttg tcgcgccgtg gcctgcgctc ctggcatccc
     7081 ggcaaaaggc cgccgggatg taaggggcaa cgccgtttcg cgcacgcgct cttcgtcgtt
     7141 gcggcgggtc cctgcccgtt gttctgtccg gtgccattca cctgtttaag cccctgtcgc
     7201 cgggggcaaa gacagccaca accgtctccg ccagtccagg ggtgaaatgc acatcccgca
     7261 aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac agtcgtttcc
     7321 ggccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa caggtgatgg tgaataccgg
     7381 agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa actcaacaga
     7441 tgatgggggc aaaaaacaga ggtgtattaa agatgggcag aaatatgtca gcagcacgta
     7501 ccgatggctt tattcgtaat attcattccc gcaatccctt tgatgttatc cgggctgatg
     7561 tggtgatcag caggctggaa aagcaggctc attggggatg tggtctgcac tatgaaattt
     7621 atgaagcgaa tttgtttgat atggcgatga atcatctttc ccggcttcct ctgaaggatc
     7681 gtccggtatt cattggtgct gctgcacgaa aggggctttt tctgacgatg gaagaaacca
     7741 ggctggcatg ggaagcgaaa gatgccctga tggctgaact tgctctggaa tactgaaaat
     7801 cgggccgcac cgtcgtcccg gatagtgctg aaatatttaa tgtggagaga atggatggga
     7861 tatatcacat atgttacaga tcaacgaccc ggtgaaccag atatcctgac cgggaacacg
     7921 tttgctgatc tggagattcg tgattctgat gggcatcttc tgctgaaagt gtctgcacct
     7981 gaagccggct ggacacacga aagcctgaac cttgttcagc cgcaggaagt gcaggagggg
     8041 aatgatgcat ttgatgctta tctgaatggt atatggatcg gttcgacaga ggtctgaaca
     8101 aatcacgcca gcagaagacg ttgctggcgt cttctgcatt cccggggagt tgtcgcgcag
     8161 catgtttgct tatacctgtg ccccgagatg attattcgtc tgcgtgcctg atgacgccgg
     8221 gcaggcggga ggataaatgc ccgtctgccc gcgccgggaa aagaccgcgc gccggtggcg
     8281 ctgaaagggg ccgcgcttac gcgccgcccc tttcattacg cggatgttct gcagtgtgct
     8341 tctgtctttt catgcctgtc atttgtctgt tactgctgat gttgttgttc ctgctactgc
     8401 tactgttctg ctttttcctg ttgctctgat tactgtctgt tttgttgttc tcctgactgt
     8461 gccttttccg ggcgtccccc tggcggggcg ggctgtcgtg aacggagcca gcgaagctgt
     8521 ctccgccctg gcgggcttcc atccctgacg cgaaccccct g
//
