LOCUS       NZ_CP148472             3126 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0006 plasmid pZ1323CEC0006-7,
            complete sequence.
ACCESSION   NZ_CP148472
VERSION     NZ_CP148472.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441573
            Assembly: GCF_037575275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 122X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037575275.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:25:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,717
            Genes (coding)                    :: 5,392
            CDSs (with protein)               :: 5,392
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 104 of 325
            Pseudo Genes (incomplete)         :: 225 of 325
            Pseudo Genes (internal stop)      :: 49 of 325
            Pseudo Genes (multiple problems)  :: 48 of 325
            Pseudo Genes (short protein)      :: 4 of 325
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148472.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0006"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0006-7"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-24"
     gene            complement(join(2763..3126,1..389))
                     /locus_tag="WHU46_RS29170"
                     /old_locus_tag="WHU46_29170"
     CDS             complement(join(2763..3126,1..389))
                     /locus_tag="WHU46_RS29170"
                     /old_locus_tag="WHU46_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249639.1"
                     /translation="MDTTSLISSISVPRLSFYETFLGCATDQEKVGAYVAYQDLSGDFF
                     CLVQMIEVALRNAINNTIKANHGPAWYDSIPQTKKSQDLVLNAKQKALDECGVRYTDDD
                     VICRLTFGFWVYMLDAPYRDTQRPCYIWTPENKAKTFPHAKNPLGGGDMKVKALFDEFH
                     KVLLFRNRLFHHEPIWKKHHCKSHSQAINNMLKEFNFLMNALSIVSNEKKELIEFIGHD
                     RRFYERCTIEYVMGIIGRIKCRELKVAG"
                     /name="hypothetical protein"
     gene            complement(848..1171)
                     /gene="mobA"
                     /locus_tag="WHU46_RS29175"
                     /old_locus_tag="WHU46_29175"
     CDS             complement(848..1171)
                     /gene="mobA"
                     /locus_tag="WHU46_RS29175"
                     /old_locus_tag="WHU46_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1457..1651
                     /locus_tag="WHU46_RS29180"
                     /old_locus_tag="WHU46_29180"
     CDS             1457..1651
                     /locus_tag="WHU46_RS29180"
                     /old_locus_tag="WHU46_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041643.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2120..2233
                     /locus_tag="WHU46_RS29185"
                     /old_locus_tag="WHU46_29185"
     CDS             2120..2233
                     /locus_tag="WHU46_RS29185"
                     /old_locus_tag="WHU46_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071790583.1"
                     /translation="MHEPPVQSDYCALSGNYRLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            2595..2738
                     /locus_tag="WHU46_RS29190"
                     /old_locus_tag="WHU46_29190"
     CDS             2595..2738
                     /locus_tag="WHU46_RS29190"
                     /old_locus_tag="WHU46_29190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338933687.1"
                     /translation="MNTKVLFVKKALKSISCATLSLLPALSRAVTELRSLSAARLKAGK
                     SF"
                     /name="hypothetical protein"
     Typing          complement(1181..1422)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.9300000000000005e-124"
     Typing          1681..2189
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.929"
                     /coverage="96.0"
                     /evalue="1.7899999999999998e-152"
     Typing          complement(852..1171)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.3e-62"
ORIGIN
        1 tagcacggcc tttgcgtatc cctatatggg gcatctaaca tataaaccca aaagccaaag
       61 gttagacggc aaataacatc atcatccgta taccttacgc cacactcatc tagtgctttt
      121 tgtttcgcat ttaaaacaag atcctgagat ttctttgttt gaggtataga gtcgtaccaa
      181 gcaggcccat gattagcttt gattgtattg tttatcgcat tacgcagagc aacctcaatc
      241 atctggacaa gacaaaaaaa atcgccagac agatcttggt aggcaacgta tgccccaacc
      301 ttttcttgat cagtagcgca cccaagaaat gtttcataga aagataatct tggtacagaa
      361 attgagctaa ttaaacttgt tgtatccatg aaaactcact tatctcttta ctatgaatat
      421 ttttcagact accattacaa cattcgagga gcaggcgaac taccgttttt ctgtgtacct
      481 ctagagcgta aaatataaca aaaaagccca ctctgtagtg ggcttttttg ttatatttat
      541 ctatatgaaa caaccagtta tagttattgt ttcataataa ggattgaata ctcttcacaa
      601 tccataacat cagaaaatac tttgctgcca atttaccata actcccctta cgcgcaccgc
      661 taaaacgcgc tgaacgcgat tcagccgcaa acaccgcaaa aagagatgtt ttacccagtg
      721 aaaactggaa aaatgcgtca ggatcgtttt cagctcactg catagctatg cataaaagtg
      781 aatggcgatc ggtttggggg cttacggcgt tcataccgtc tgttttcgac agtttctctc
      841 cgggaagcta atctgccata agcctggata acagggcacg gtgatactcc gtaatagcga
      901 tcagcacctc cgcatactcc ctgtccccga cacgcttgcc gtcgataaag agttttttct
      961 gtaacgcccc cagtctcata acttctttca gtacccgatc atcagtcagt gagttaactt
     1021 tcttaccgag agctgccgcc cgtaaaaaac cggaaacggt cttgccgcat tcagcagccc
     1081 tttttctgat ttcctggtct tcttctgccg tcagacgaac agggcgactg atagcccgcc
     1141 tgcgcgtatt acttccactt cgttttgtca taaaacatgg ctccgtatct gacatggggt
     1201 gtcggggcga agccctgacc agggtaattg taatagcgtg catgtatgcg cggtataaca
     1261 attacacatc ctgtcctgtc agcaagttcg aattaccgac aaaggcacgg cagttttttt
     1321 ggcagtgaca gtattgtcag gcagaaatgc ccgtcaaaga gtcagacggc agaataccgg
     1381 cggcaaaact gtcgaaaagt ggtacgcgat ataatcacat aacgcagatg acagacagaa
     1441 cggagaaaac agaaagatga acaagcagca gcaaactgta ctcaacatgg cgggcttcat
     1501 aaaaagccag agcctgacac tgctcgaaaa gctggacgca ctcgatgccg acgaacaggc
     1561 cgccatgtgt gagaagctgc acgaactcgc ggaagaactc cagaacagca tacagacacg
     1621 ctttgaagta gaaaatagca cagagatata acgctcaggg caggatgccg aacggcacaa
     1681 cagagcgtaa ggagaaaatg tcacataagg cgctcttccg cttcctcgct cactgactcg
     1741 ctacgctcgg tcgttcgact gcggcgagcg gtgtcagctc actcaaaggc ggtaatacgg
     1801 ttatccacag aatcagggga taaagccgga aagaacatgt gagcaaaaag caaagcaccg
     1861 gaagaagcca gcaccgcagg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1921 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga cttaaagata ccaggcgttt
     1981 ccccctggtg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     2041 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     2101 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactact gcgccttatc
     2161 cggtaactat cgtcttgagt ccaacccgga aaggcacgac ttatcgccat tggcagcagc
     2221 cactggtaac tgatttagag gagttagtct tgaagttacg tgccagttaa ggctaaactg
     2281 gaataacaag ttttggtgac tgcgctcctc ttaagccagt taccgcggtt cagaaacttg
     2341 ccggttgctg aaccttcgaa aaaccacctt gccgggtggt tttttcgttt ttaagattac
     2401 gatgggcgcg atatgaactc aaggaaatcc gtaattaaat tataaaactt cgtgaattaa
     2461 tggaaaaact gatgtgaatc agctatttac ccttaagatt tgatgttaga tatggtataa
     2521 ttaaccattc aaatagatgg aataaaaagt caacgctgcc attagaacgg cataagaaat
     2581 agaggattat gaaaatgaat actaaagttc tttttgtgaa aaaagctttg aaatcaatta
     2641 gttgcgcaac tctttcgctc ttaccggctt taagtcgcgc agtaacagag ttacgtagcc
     2701 tttcggctgc gcgacttaaa gccggtaaga gcttttgatt gtatcacatt tttccttaaa
     2761 agtcaaccgg ctaccttaag ctccctgcac ttaatcctcc ctataatccc cataacatac
     2821 tctatcgtgc agcgttcata aaagcgccta tcgtgcccaa taaactcaat aagttccttt
     2881 ttctcgttag agacgatact caaggcattc attaaaaaat tgaactcttt caacatatta
     2941 tttatggctt gactatgcga cttgcagtgg tgttttttcc aaataggctc atggtgaaaa
     3001 agtcgattcc taaacaaaag aactttatga aactcgtcaa acagcgcttt aaccttcata
     3061 tcccccccac ccaaaggatt cttcgcatga ggaaatgttt ttgccttgtt ctctggagtc
     3121 cagatg
//
