LOCUS       NZ_CP148469             8214 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0006 plasmid pZ1323CEC0006-4,
            complete sequence.
ACCESSION   NZ_CP148469
VERSION     NZ_CP148469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441573
            Assembly: GCF_037575275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 122X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037575275.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:25:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,717
            Genes (coding)                    :: 5,392
            CDSs (with protein)               :: 5,392
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 104 of 325
            Pseudo Genes (incomplete)         :: 225 of 325
            Pseudo Genes (internal stop)      :: 49 of 325
            Pseudo Genes (multiple problems)  :: 48 of 325
            Pseudo Genes (short protein)      :: 4 of 325
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148469.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0006"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0006-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-24"
     gene            complement(join(8112..8214,1..803))
                     /locus_tag="WHU46_RS29075"
                     /old_locus_tag="WHU46_29075"
     CDS             complement(join(8112..8214,1..803))
                     /locus_tag="WHU46_RS29075"
                     /old_locus_tag="WHU46_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_338933686.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAAYSALFDHGLASK
                     SDQRFASIRSTGNLTFSTAF"
                     /name="type II restriction endonuclease"
     gene            874..2013
                     /gene="dcm"
                     /locus_tag="WHU46_RS29080"
                     /old_locus_tag="WHU46_29080"
     CDS             874..2013
                     /gene="dcm"
                     /locus_tag="WHU46_RS29080"
                     /old_locus_tag="WHU46_29080"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_033550328.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAIMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            complement(2379..2612)
                     /locus_tag="WHU46_RS29085"
                     /old_locus_tag="WHU46_29085"
     CDS             complement(2379..2612)
                     /locus_tag="WHU46_RS29085"
                     /old_locus_tag="WHU46_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2619..3137)
                     /locus_tag="WHU46_RS29090"
                     /old_locus_tag="WHU46_29090"
     CDS             complement(2619..3137)
                     /locus_tag="WHU46_RS29090"
                     /old_locus_tag="WHU46_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3175..3873)
                     /locus_tag="WHU46_RS29095"
                     /old_locus_tag="WHU46_29095"
                     /pseudo=""
     CDS             complement(<3175..3873)
                     /locus_tag="WHU46_RS29095"
                     /old_locus_tag="WHU46_29095"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3815..4138)
                     /locus_tag="WHU46_RS29100"
                     /old_locus_tag="WHU46_29100"
     CDS             complement(3815..4138)
                     /locus_tag="WHU46_RS29100"
                     /old_locus_tag="WHU46_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4202..4393
                     /locus_tag="WHU46_RS29105"
                     /old_locus_tag="WHU46_29105"
     CDS             4202..4393
                     /locus_tag="WHU46_RS29105"
                     /old_locus_tag="WHU46_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5063..5224
                     /locus_tag="WHU46_RS29110"
                     /old_locus_tag="WHU46_29110"
     CDS             5063..5224
                     /locus_tag="WHU46_RS29110"
                     /old_locus_tag="WHU46_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(5416..5634)
                     /locus_tag="WHU46_RS29115"
                     /old_locus_tag="WHU46_29115"
     CDS             complement(5416..5634)
                     /locus_tag="WHU46_RS29115"
                     /old_locus_tag="WHU46_29115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338933685.1"
                     /translation="MPNHDRFECQTAIDSDAILQSAMELSRKWNNVSYTDSEKEEIQQS
                     ILKQIEKYSDFPYVVNYYRNRLSALFD"
                     /name="hypothetical protein"
     gene            5808..7343
                     /gene="istA"
                     /locus_tag="WHU46_RS29120"
                     /old_locus_tag="WHU46_29120"
     CDS             5808..7343
                     /gene="istA"
                     /locus_tag="WHU46_RS29120"
                     /old_locus_tag="WHU46_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_023181050.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSLS
                     WPLPADISHDTLEKLIFPPKDTFASELVMPDMLYLDTEMRKPGVTRQLLWMEYKAQAGN
                     KAMGYSHFCRCYREWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFV
                     AVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVI
                     NDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRILDAPALSPLPLAPYEYTEYKAVKVGPDYHVEYA
                     RHWYSVPHELVGQRLSLKAGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHRQH
                     GTWTPERLIEQGKRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYGPERLEKAC
                     YVALHYNAPDRRFIDNLLRHHRENLELPLSRQGEQHPAYASEHENLRGPGYYH"
                     /name="istA"
     gene            7360..8115
                     /gene="istB"
                     /locus_tag="WHU46_RS29125"
                     /old_locus_tag="WHU46_29125"
     CDS             7360..8115
                     /gene="istB"
                     /locus_tag="WHU46_RS29125"
                     /old_locus_tag="WHU46_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="WP_001282653.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLVD
                     DELLSRENSRVARLRKNAGLKYQATPEGLRYPASRGLRAEQMRELLNGHYITHRKNLLI
                     TGPTGCGKSWIANALGEQACRQKHSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQKIQV
                     LILDDLGLEQLSNAQCNDLLELIEDRYGQSSTIVVSQFPVDKWHGLMENPTTADAILDR
                     LVHNSHRVVLQGESLRKNPPVVESSEKTR"
                     /name="istB"
     Typing          complement(4479..4566)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          4590..5135
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.353"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2276..3825)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgactgta gcatatcttc aaaggttagc accctattat tatttttata tttgaaattt
       61 ttattttcta tcgtcgttac aacgaccaca ttagcctcgt acaatatatt tattgtctcc
      121 tcgctaaaag aatcatctag tgtagctaga tacatttctc taattcctgt tctattaacc
      181 tcttcaggta cttcttgcca tcgctccctc agcgttgttt tagcggaaat caacattgta
      241 tttcttttgt tagatgtgta ctggactaca cctggcataa caagatctac aagtttacct
      301 atttggtttt tttgaaaaaa tgacttccca atagccccct gcacatcaac aggaattcct
      361 gcccccatca ttaatagctc aagaatagac tcaaactctt ttcctgccct gctacgtctg
      421 gattgagtgt tagacagtgc caaatcatat atgtgtgtag ggtattccat cgtaaaatca
      481 cgaatggcat cataaggact catatctttt attctttctt catcaataag atatgataac
      541 attcttgttg tgaattcttt ttcaaatggt tcaaattctt tccaacagtt ttttctcatt
      601 gcaattatat attcacttgg attattcaaa aaataatcac tatttttttg atcataccct
      661 aaatcacaaa atgttttata aaccaaagca aatggcgtaa taaaatgcga ctttctctct
      721 ttagaaatta gagttttaaa ttcaccagga ctcaatcttt gcataaaaaa taacctttta
      781 tatctacttg aaagtttttt catgcacgtt cataattgga atcaaaacag gacaaattgt
      841 cctaaaacca acacttaatt ctggtagaca tgcatgactg ataatatcgc agcaacaatc
      901 aaaggaaaaa gagaaagact tcatatgact caaaaagagt ttgcagacgc ccttggtctt
      961 tcaaaatatg gagacagaac cataagaaga tgggagcgcg gggaaacaaa acctactggg
     1021 gcggaactaa aagcaattat ggactttcca gacacaccac cttacccaaa caacgagaat
     1081 ggcagatatc gcatgatcga tttatttgct ggaatcggtg gaaccagact tggatttcat
     1141 caaactaacg ctgtaaatgt tgttttttcc agcgaatggg ataagtttgc tcaaaagaca
     1201 tatcatgcaa attatggtga tttccctgat ggggacataa caaaaatcga tgaaaaagat
     1261 attcctgatc atgaaattct tgtcggtggg tttccttgcg tagcattctc tcaagcaggg
     1321 ctcaaaaaag gatttaatga tacaagagga actttgtttt ttgatatagc aaggataata
     1381 aaggaaaaaa aacctcatgc ttttttactt gaaaatgtaa aaaacttgct aggacatgat
     1441 aaaggtagaa ctttttcaat aataaaaaac acacttgaag aattgaatta cactgtgtat
     1501 tacaatatct tcgctgcaaa agactttggt gttccacaaa atagagaacg catatacatt
     1561 gtaggcttta ataaagaaaa agtaagaaat catgaacatt ttacttttcc aaccccgtta
     1621 aaaacaaaaa caagagttgg agatattctt gaaaagagcg tagacaataa atatacgcta
     1681 tcagatgcgc tctggaatgg acaccaacgc aggaaacttg taaatgcagc agccggtaaa
     1741 ggctttgggt atggtttatt taatgaaaat agcccatata ctaataccat aagtgcgagg
     1801 tactataaag atggtagtga aatattaata gagcaaaaag gaagtaaccc aagaaaaata
     1861 actccgcggg aagcgtcaag acttcaaggg tttcctagtg attttattat ccctgtatcg
     1921 gatacacagg cttataagca atttgggaat tcagtcgcag tccctgttat caatgcaata
     1981 gcagaaaaaa tcatatcgac cttagattca taaactaagt cataaggaca caatgcggcc
     2041 cagaatccaa ttaccccccc atatggattt accataacgt cgcattacgc gcaccgctga
     2101 aacgcgttca gcgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa
     2161 actgccgcga tcgcgcctga taaattttaa ccgcatgaat acctatgcaa ccagatggta
     2221 caggccacat taacccctct taatccactg aagctgccat ttttcatggt ttcaccatcc
     2281 cagcgaagga ccatccagcg tgcgttcctg tatttccggc tgacgctccc gttccaggga
     2341 taacgcatgt tcgcgctcct gtatcagccg ttcctctctt atctccagtt ctcgctgtat
     2401 aactggctca agcgttctgt ctgctcgctc aagtgctgca cctgctgact caactgcatg
     2461 acccgctcgt tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatctt ctgcaattcc
     2521 acgaaggcgc tctcccattc gctcagccgc tgcatatagt cctgttgcag ctgctctaag
     2581 gcgttcagca aatgtatttc cagctctgtc actctgtgtc actccttcag atgtacccac
     2641 tctttcccct gaaagggaat cacctccgct gatttcccgt acggaaggac aaggaatttc
     2701 ctgttcccgt cctgcacaaa ctccacgccc catgtcttcg cgttcagttt ctgcaatgtc
     2761 tcttcctgct tcctgatttc ttccaggttc gtctgtatcc gtccgccaag ataccagagc
     2821 gtcccgccac tcgcggtaaa caggagaaag actatcccca gtaacatcat gcccgtattc
     2881 cctgccagct ttaacacgtc cttcctgtgc tgcatcatcg cctctttcac cccttcccgg
     2941 tgtttttcca gcgatttctc tgtcgaggct gtgaacaggg ctatagcgtc tctgattttc
     3001 gtctcgtttg aggtcacagc ctcgctgaca gactcgccga gcttcctgaa ctcgtcgttc
     3061 agcattttct ctgtagattc ggctctctct ttcagctttt tctcgaactc cgcgcccgtc
     3121 tgtaaaagat tgctcataaa acgctccttt cagtctgata ttcctcccgc cgttcgggtc
     3181 tgcaatgctg atactgcttc gcgtcaccct gaccacttca agccccgcct ctgtgagcgc
     3241 ctgaatcaca tcctgacggc cttttatctc tccggcatgg taaagtgcgt ctataccgcg
     3301 tgtaacgctc tcagcaagtg cctgtttcgt ttccggcagg ttatcaggaa gagtcagcgt
     3361 ccggcggttc tccggggcgt tggggtcatg cagtccgtaa tggtgattca ccagcgtctg
     3421 ccaggcatca attctcggcc tgtctgcgcg gtcgtagtac ggctggagac gttttccggt
     3481 ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc
     3541 cacccacaga atgctgtact gatttttttc gagaccgggc atcagtacac gctcaaagct
     3601 cgccatcact ttttcacgtc ctcccggcgg cagctccttc tccgcgaacg acagaacacc
     3661 ggacgtgtat ttcttcgcaa atggcgtggc atcgatgagt tcccggactt cttccggatt
     3721 accctgaagc accgttgcgc cttcgcggtt acgctccctc cccagcaggt aatcaaccgg
     3781 accactgcca ccaccttttc ccctggcatg aaatttaact atcatcccgc gccccctgtt
     3841 ccctgacggc cagacgcagc cggcgcagct catccccgat ggccatcagt gcggccacca
     3901 cctgaacccg gtcaccggaa gaccactgcc cgctgttcac cttacgggct gtctgattca
     3961 ggttatttcc gatggcggcc agctgacgca gtaacggcgg tgccagtgtc ggcagtttcc
     4021 cggaacgggc aaccggctcc cccaggcaga cccgccgcat ccataccgcc agttgtttac
     4081 cctcacagcg ttcaagtaac ctggcatgtt catcatcagt aacccgtatc gtgagcatcc
     4141 tctcgcgttt catcggtatt attaccccat gaacagaaat cccccttaca cggaggcatc
     4201 agtgactaaa caggaaaaaa cagcccttaa catggcgcgc tttatcagaa gccagacatt
     4261 aacgctgctg gaaaagctca acgaactgga cgcggatgaa caggcagaca tctgtgaatc
     4321 gcttcacgac cacgccgatg agctttaccg cagctgcctc gcacgtttcg gggatgacgg
     4381 tgaaaacctc tgacacatgc agctcccaga gacggtcaca gcttgtctgt gagcggatgc
     4441 cgggagcaga caagcccgtc agggcgcgtc agcaggtttt agcgggtgtc ggggcgcagc
     4501 cctgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagtg
     4561 cggattgtat gaaaagcacg ccatgccggg tgtgaaatgc cgcacagatg cgtaaggaga
     4621 acataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4681 cggctgcggc aagcggtgcc ggctcactca aaggcggaaa tacggttatc cacagaatca
     4741 ggggataagg ccggaaagaa catgtgagta aaaaggcaaa gggcaacaaa aggccgcgcc
     4801 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4861 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4921 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4981 ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg tatctcggcc cggtgtaggt
     5041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5101 atccggtaac tatcgtcttg agtccaaccc agtaagacac gccttaacgc cactggcagc
     5161 agccactggt aaccggatta gcagagcgag atttatccag atgcttacag agttcttgaa
     5221 gtgatggcct aactacggct acactggaag gacagtattt ggtatctgtg ctctgctgaa
     5281 gccagattac ctcggttaga taatctgcca ggattagcta accttcgaaa aaaccacctg
     5341 ccagggtggt tttttcgttt acaggtccaa gattacgcgc agaaaaaaag gatctcaaga
     5401 gatcctttta gttttttagt caaaaagagc actaagccta tttctataat aattaacaac
     5461 ataagggaaa tcactatatt tttctatttg ttttaatatt gactgttgaa tttcctcttt
     5521 ttcagagtct gtgtaactta cattattcca ctttcttgat aattccattg ctgactgcaa
     5581 aatggcatct gaatcgatcg cagtttggca ttcgaatcga tcatgatttg gcatccatct
     5641 ccgatcacag tttggcatca caatcgatca cgatttggca tgcttccgat cattgattgg
     5701 catcctgcca gtcactccgg gaattaactc ttttcaccac agtctccatt gccgtgttta
     5761 aaccagtgga gacgtcaatg tccagaaaga gaaaatccag gagcactatg gaaacctgtt
     5821 ttaagattct tcagctcaag ttcgacaaga agctgactaa ccgttgtatc ggactgacgt
     5881 tacacatcag cgcctcaacc gtttttgagg ttcttgcccg cttcaaagcc tcttctctgt
     5941 catggccatt acctgctgat atatcccatg acacgctgga gaaactcatt tttccgccca
     6001 aagacacctt tgcatcagag cttgtaatgc ctgacatgtt gtacctcgat acggaaatga
     6061 gaaaaccagg tgtgacgcgg caactgttgt ggatggaata caaggcacag gcgggtaaca
     6121 aagcgatggg atactcgcac ttctgccgtt gctaccggga gtggaaaaaa acgcgacgtc
     6181 tctccatgcg ccaggagcat cgtgccggcg aaaagctttt tatcgacttc tgtggtccta
     6241 cggttcctgt tattaatccg gacaccggtg agatacgccg cgtggcaatc ttcgtggccg
     6301 taatgggggc gtccaactat acctacgttg aagcgtgcga aggtcaggac atgatgtcat
     6361 ggttaaatgc ccacagccgt tgcctgacct tcctgggcgg cgtaccgaag ctgctcatcc
     6421 ccgataacct cagaagcgca gtgaaaaagg ccgatcgata tgagcctgtc atcaacgaca
     6481 gttatcaggc actggctgag cactatggca cggttatcat cccggcccga ccacgcaaac
     6541 ctaaagataa accaaaagca gaaaacggcg tcctcatcgt cgaacgatgg cttcttgccc
     6601 gcatccgtaa tgaaaccttc cacacgctca gggcgctgaa tgcgcgcctt agggagttac
     6661 tgacagacat gaataaccgg ccaatgaagg ggtacggcaa tcaaacccgt gcagaacgct
     6721 tccgcatact ggatgcgcca gcactctctc cgctgcctct ggcaccttat gaatacacgg
     6781 aatacaaagc tgtgaaggtt ggccctgact accacgttga atatgcccgt cactggtact
     6841 ccgttccaca cgaactggtc ggtcagcgtc tgtcactgaa ggcggggcag tcggtggtac
     6901 agctctggca caaggggcag tgtgtggcgc aacatccgcg cagcacacat gaatataaac
     6961 acacaaccaa tccgcttcac atgccagagc gccaccggca gcacgggaca tggacaccgg
     7021 aacgcctgat cgagcaggga aaacgtacgg gcccgtccac aggccgggtg gttgaatcca
     7081 tgctgaaggc aaaaccccat ccggaactgg cttatcgtgc agttctgggg ctgctggcac
     7141 ttcagaaaaa atacggcccg gaacggctgg aaaaggcctg ttacgttgcc ctgcactata
     7201 acgcccctga cagacgcttt atcgacaatc tgctgcgtca ccatcgggag aacctggagc
     7261 ttccattatc ccgccagggc gaacaacatc cggcatatgc ttcagagcat gaaaacctgc
     7321 gtggtccggg ctactaccac tgacagaagg aacaacagta tgagtgataa cctgttaaac
     7381 aaactgaccc aactgaaact cccggcgatg gccgggagcc tgatacggca gcgggaaacg
     7441 cctcagacct acgacgaact gtcgttcgag gaaaggctga cgctgctggt tgatgacgag
     7501 cttctgagca gggaaaacag ccgtgtggca cggttgagaa aaaatgccgg cctgaaatac
     7561 caggcaacac cggaagggct gcgctatccg gcctcaagag gtctgcgggc tgaacagatg
     7621 agagaactgc tgaacggcca ctacataacc cacaggaaaa acctgctgat aaccggtccg
     7681 acaggatgtg gcaagagctg gatagccaat gcgctgggcg aacaggcatg tcgacagaaa
     7741 cacagcgtcc ggtactgtag aaccgggcga ttactggaac aactggctca gggacgtgtt
     7801 gatggcagct ggctgaaata tctgaaacag ttgcaaaaaa tacaggttct aatcctggac
     7861 gatctggggc tggaacagtt aagtaacgcc cagtgtaatg accttctgga gctcatagag
     7921 gatcgttacg gacaaagcag cacgatagtg gtaagccagt tcccggtaga taaatggcat
     7981 ggtctgatgg agaacccaac aacagcagat gccatcctgg acagactggt acataactca
     8041 caccgggtgg tccttcaggg agagtcactg aggaaaaatc ccccggtagt ggaaagcagc
     8101 gaaaaaacgc gttaaaaagc ggtagaaaaa gttaagttcc cggttgatcg aatggatgcg
     8161 aatcgctgat cggacttcga tgccaatccg tgatcgaata gagcggaata cgca
//
