LOCUS       NZ_CP148459             3589 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0009 plasmid pZ1323CEC0009-7,
            complete sequence.
ACCESSION   NZ_CP148459
VERSION     NZ_CP148459.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441576
            Assembly: GCF_037573545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037573545.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:20:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,781
            CDSs (total)                      :: 5,650
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 330
            CDSs (without protein)            :: 330
            Pseudo Genes (ambiguous residues) :: 0 of 330
            Pseudo Genes (frameshifted)       :: 117 of 330
            Pseudo Genes (incomplete)         :: 210 of 330
            Pseudo Genes (internal stop)      :: 51 of 330
            Pseudo Genes (multiple problems)  :: 46 of 330
            Pseudo Genes (short protein)      :: 4 of 330
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148459.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0009"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0009-7"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-27"
     gene            join(3471..3589,1..205)
                     /gene="mobA"
                     /locus_tag="WHU68_RS28880"
                     /old_locus_tag="WHU68_28880"
     CDS             join(3471..3589,1..205)
                     /gene="mobA"
                     /locus_tag="WHU68_RS28880"
                     /old_locus_tag="WHU68_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            638..2155
                     /locus_tag="WHU68_RS28885"
                     /old_locus_tag="WHU68_28885"
     CDS             638..2155
                     /locus_tag="WHU68_RS28885"
                     /old_locus_tag="WHU68_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_072652832.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRST
                     DIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYESVLKNLLSAKILRSVYIDGVGDCVLINNNIANDISIFNM
                     ANCESFFDKPIFELIKAWLRNLGIVAFNQIKTKYDGENNPVVGSFEWDITAPSYVSPLA
                     EYSSDGLIPGFVVCDFALGFNRNEITTDAADTFIRKVQMTKSSRTNQRIMFVLFARRFE
                     KNAFNKLRSEGVLAITIANAFGNKVDESLARLSKVIQGTLSIERHPDELLQMVKDLESI
                     SGENGNLRGYIFELFVSSQICNFYGYGNVRINKEYKINGKHAEADVVLETNDDIYIVEC
                     KNVKTLPSMEVSLWMKTRVPIINSYYKSNNPDNKNIHHRLWVTGNIYPKDINRLDEFKC
                     NNKKIDVDYLFGQSLDDFFYQKKQVFNLYRKVISPDYKKGQIPDFDL"
                     /name="NERD domain-containing protein"
     gene            complement(2360..2518)
                     /locus_tag="WHU68_RS28890"
                     /old_locus_tag="WHU68_28890"
     CDS             complement(2360..2518)
                     /locus_tag="WHU68_RS28890"
                     /old_locus_tag="WHU68_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532985.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPKRHDLSPLAAATGNWNSRAMIM
                     RSVLQSS"
                     /name="replication initiation protein"
     gene            complement(3005..3199)
                     /locus_tag="WHU68_RS28895"
                     /old_locus_tag="WHU68_28895"
     CDS             complement(3005..3199)
                     /locus_tag="WHU68_RS28895"
                     /old_locus_tag="WHU68_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041642.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3237..3462
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="99.0"
                     /evalue="4.9e-75"
     Typing          complement(2451..2962)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.143"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          3..202
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.507"
                     /coverage="99.0"
                     /evalue="2.49e-34"
ORIGIN
        1 tggtttttta cgggcggcag ctctcggtaa gaaagttaac tcactgactg atgaccgggt
       61 gctgaaagaa gtcatgcgac tgggggcgtt gcagaaaaaa ctctttatcg acggcaagcg
      121 tgtcggggac agggagtatg cggaggtgct gatcgctatt acggagtatc accgtgccct
      181 gttatccagg cttatggcag attagcttcc cggggagaaa actgtcgaaa acagacggta
      241 tgaacaccgt aagctcccaa agtgatcacc attcgctttc atgcataact atgcagcgag
      301 ctgaaaacga tcctgacgca tccttcctat ttttccgggg taaaacatct ctttttgcgg
      361 tgtctgcgtc agaatcgcgt tcagcgcgtt tcagtggtgc gtacaattaa gggattatgg
      421 taaatagggc ggttgatata tgatgatgaa atgtcacaaa cgtaacatct tgatttttaa
      481 ggataaatct cgcaggtgtg ttttttgtga tatgttgctt ttgttaaata tttgattttt
      541 atgatttttt tctttgtgtt ttttttttgg gatctataga tattacacgt agtgtaattt
      601 tataaatagc gtagtgtgac aaaaataaga tgaatttatg aaaaaaaaca ttgttagaca
      661 agtgcttgag agttatggtc cgtgcatatc ttctgaactt gctgagagga ttaagcgtca
      721 gaatccttct atgtcatctg atgcaatacg taaaatgatc tctcgaagca ctgatattgg
      781 taagttgcca tttttaagat ttagtcataa caggagattc atttatctaa aagatgactt
      841 tggtagcttc aaattttggc gggcgctcaa aaaatgtatg cgtgaggcaa attcggcgta
      901 ttctcacgct atactttccg taattaataa tggcggatac ttgaaggtaa aggatttcgg
      961 gattgtgtct ggctctccga taaagcaggc caagcattta agttatgaat ctgttctaaa
     1021 aaatctgctt tcagctaaaa tacttagatc tgtgtatatt gatggtgttg gtgattgtgt
     1081 tttgataaat aataatatag ctaatgatat cagcatcttt aatatggcaa attgcgagtc
     1141 tttttttgat aagccaatat ttgaactgat taaggcatgg ttgcgaaatt taggtattgt
     1201 ggcttttaat caaataaaaa caaaatatga tggagagaac aatcctgtgg ttggttcttt
     1261 cgagtgggat ataacagcgc caagttatgt gtcccctctt gctgaatatt ccagcgatgg
     1321 actcatccca ggttttgttg tctgtgattt tgctctaggg tttaatcgaa atgaaattac
     1381 caccgatgct gctgatacct tcataagaaa agttcagatg accaagtcct ctaggacaaa
     1441 ccagaggatt atgtttgttc tttttgctag acgttttgag aagaatgcct tcaacaagct
     1501 aaggagtgaa ggggttctgg ctataactat agctaatgcg tttggtaata aggtcgatga
     1561 gagcttggcc agactatcga aggtgattca gggaacttta tctattgaaa gacatcctga
     1621 tgagttactt caaatggtaa aagatctgga gtctatctct ggagagaatg gaaatttaag
     1681 ggggtatatt tttgagcttt ttgtttcctc tcaaatttgt aatttctatg gttatggaaa
     1741 tgttcgtatt aataaagaat ataaaataaa cggtaaacat gccgaagctg atgttgtatt
     1801 ggaaactaac gatgatattt atatcgttga gtgcaaaaat gttaagactt taccttcgat
     1861 ggaggttagt ctttggatga aaacaagagt gccaattata aattcctact acaaatcaaa
     1921 taaccctgat aacaaaaata tacatcatcg tttgtgggtt acggggaata tatatcctaa
     1981 agatataaac cgattggacg agtttaaatg taataataag aagattgatg ttgattattt
     2041 atttggtcag tctttggatg attttttcta ccaaaaaaaa caagtgttta atttatatag
     2101 gaaagtcatt tcgccagatt ataaaaaagg gcaaataccc gattttgact tatagaatga
     2161 cgttttctag tctctgcatt tcttgacgat gacggcttga aaagcgtatc ttcctccatg
     2221 aaacgaaaaa accacccggc aaggtggttt ttcgaaggtt agttaatcct ggcagattac
     2281 ctaacctagg taactggctt cagcagagcg cagataccaa atcctgtcct ttaagtgtag
     2341 ccgtagttgg ggcatcactt caagaactct gtagtacgga ccgcattatc atcgctctgc
     2401 tattccagtt accagtggct gctgccagtg gcgataagtc gtgtcttttc gggttggact
     2461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2521 agcccagctt ggagcgaacg acctacaccg ggccgagata ccaacagcgt gagctatgag
     2581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaaac ggcagggtcg
     2641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2701 tcgggtttcg ccacctctgg cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2761 gcctatggaa aaacgccacc ggtttcgggt tcttgttttt tgctcacatg tttcttttgg
     2821 ctttatcccc tgattctgtg gataaccgca ttaccgtctt tgagtgagct gataccgctc
     2881 gccgcagccg aacgaccgag cgtagcgagt tagtgagcga ggaagcggaa gagcgcctta
     2941 tgtggcattt tctccttacg caccggtata ccgttctgca tcctgtctgg agcaggatac
     3001 actgttatac cccggtgcca ctttccgctt caaagcgtgt ctgtatgctg ttctggagtt
     3061 cttccgcgag ttcgtgcagc ttctcacaca tggcggcctg ttcgtcggca tcgagtgcgt
     3121 ccagcttttc gagcagcgtc aggctctggc tttttatgaa tcccgccatg ttgagtacgg
     3181 tttgctgctg cttgttcatc tttctgtttt ctccgttctg tctgtcctct gcgtcgtgtg
     3241 attatatcgc gtaccacttt tcgaccgtct taccgccggt attctgccga cggacatttc
     3301 agtcagacaa cactgtcact gccaaaaaac agcagtgctt tgttggtaat tcgaacttgc
     3361 agacaggaca ggatgtgcaa ttgttatacc gcgcatgcat gcacgctatt acaattaccc
     3421 tggtcagggc ttcgccccga caccccgtgt cagatacgga gccatgtttt atgacaaaac
     3481 gaagtggaag taatacgcgc aggcgggcta tcagtcgccc tgttcgtctg acggcagaag
     3541 aagaccagga aatcagaaaa agggctgctg aatgcggcaa gaccgtttc
//
