LOCUS       NZ_CP148457             4936 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0009 plasmid pZ1323CEC0009-5,
            complete sequence.
ACCESSION   NZ_CP148457
VERSION     NZ_CP148457.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441576
            Assembly: GCF_037573545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037573545.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:20:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,781
            CDSs (total)                      :: 5,650
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 330
            CDSs (without protein)            :: 330
            Pseudo Genes (ambiguous residues) :: 0 of 330
            Pseudo Genes (frameshifted)       :: 117 of 330
            Pseudo Genes (incomplete)         :: 210 of 330
            Pseudo Genes (internal stop)      :: 51 of 330
            Pseudo Genes (multiple problems)  :: 46 of 330
            Pseudo Genes (short protein)      :: 4 of 330
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148457.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0009"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0009-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-27"
     gene            273..791
                     /locus_tag="WHU68_RS28825"
                     /old_locus_tag="WHU68_28825"
     CDS             273..791
                     /locus_tag="WHU68_RS28825"
                     /old_locus_tag="WHU68_28825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_112016408.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKDAIALFTTSTEESLKKHREGVKEAMMQHRKDMLKLAGSSGMRLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRVQEETLRKLNAKTWGVEFVQDGRRKFLVLPYGKSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            798..1031
                     /locus_tag="WHU68_RS28830"
                     /old_locus_tag="WHU68_28830"
     CDS             798..1031
                     /locus_tag="WHU68_RS28830"
                     /old_locus_tag="WHU68_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_096987281.1"
                     /translation="MTELETHLLNALKQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSGQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1642..1938)
                     /locus_tag="WHU68_RS28835"
                     /old_locus_tag="WHU68_28835"
     CDS             complement(1642..1938)
                     /locus_tag="WHU68_RS28835"
                     /old_locus_tag="WHU68_28835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_138650211.1"
                     /translation="MERKHKGKCPFCNSEMAPEVIEKNTIRRDKCKCTTCGEIIYKCRN
                     IFCNDYAKGGLLYDDELCPPCGEGLLKAVKEFPDKYRAAIQKVVEEKNREKNN"
                     /name="hypothetical protein"
     gene            complement(2013..2168)
                     /locus_tag="WHU68_RS28840"
                     /old_locus_tag="WHU68_28840"
     CDS             complement(2013..2168)
                     /locus_tag="WHU68_RS28840"
                     /old_locus_tag="WHU68_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="WP_072210546.1"
                     /translation="MNDKWEFYLDNSKEWRWRRTASNGRIVGASSQGYVNKSDCIDNAR
                     RHGYNG"
                     /name="DUF1508 domain-containing protein"
     gene            complement(3135..3293)
                     /locus_tag="WHU68_RS28845"
                     /old_locus_tag="WHU68_28845"
     CDS             complement(3135..3293)
                     /locus_tag="WHU68_RS28845"
                     /old_locus_tag="WHU68_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071777339.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(3952..4143)
                     /locus_tag="WHU68_RS28850"
                     /old_locus_tag="WHU68_28850"
     CDS             complement(3952..4143)
                     /locus_tag="WHU68_RS28850"
                     /old_locus_tag="WHU68_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4208..4531
                     /locus_tag="WHU68_RS28855"
                     /old_locus_tag="WHU68_28855"
     CDS             4208..4531
                     /locus_tag="WHU68_RS28855"
                     /old_locus_tag="WHU68_28855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3790..3874
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(3222..3779)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1129
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.149"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4522..4934
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="9.04e-84"
ORIGIN
        1 taatcaccat tacgggctgc atgaccccaa cgccccggag aatcgccgga cgctgacact
       61 ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcgttacac gaggcctgga
      121 ggtgctttac cattccggag agataaaagg ccgtcaggat gtgattcagg cgctcacaga
      181 ggtggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag acccgaacgg
      241 cgggaggaat atcagactga aaggagcgtt ttatgagcaa tcttttacgg acgggcgcgg
      301 agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg aacgacgaat
      361 tcagcaggct cggcgaatct gtaagcgagg ctgtgacctc aaacgagacg aaaatcaaag
      421 acgctatagc cctgttcaca acgtcgacag aggaatcgct gaaaaaacac cgggaagggg
      481 tgaaagaggc gatgatgcag caccggaagg acatgttaaa gctggcaggg agttcgggca
      541 tgaggttgct ggggatagtc tttctcctgt ttaccgcgag tggcgggacg ctctggtatc
      601 tcggcgggag gatacagacg aacctggaag aaatcagggt acaggaagag acattgcgga
      661 aactgaacgc gaagacatgg ggtgtggagt ttgttcagga cgggagaagg aaatttctgg
      721 tccttccgta cgggaaatca gcagaggtga ctcactatca ggggaaggac tggatacaac
      781 tgacggagtg atacggaatg acagagctgg aaacacattt gctgaacgcc ttaaagcagc
      841 tgcaacagga ctatatgcag cggctgaacg aatgggagag cgccttcgtg gaattgcaga
      901 agatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc atgcagttga
      961 gtcagcaggt gcaacacttg agcgggcaga cagaacgctt gagccagtta tacagcgaga
     1021 acaggagata agggatgaac ggctgatgca ggagcgcgaa catgcattat ccctggagcg
     1081 ggaacgccag ctggaaatac aggaacgcac actggatggt ccttcgttgt cgctgtgatg
     1141 gcgaaactat gaaaaatggc aggttcggtg gattttgacg ggctaatgtg gtctgcacca
     1201 tctggttgca taggtattca tacggttaaa atttatcagg cgcgatcgcg gcagtttttc
     1261 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgatgaac gcgttttagc
     1321 ggtgcgtaca attacagggt tatgttaaat tgatggtttg ataagtgttg gaaataatta
     1381 attccactca taaatataca agatatgaaa aaaatattgt tcatatggta gaagagctat
     1441 agggatattt ggtttcaaaa tattcctttt aggaaatgga ttaactaaaa caggtactaa
     1501 aatacctgtt aaaaataatg agagagataa ctctctgtta gggcgtgtgt tggggatata
     1561 gaaatatttg gtattgctta cgaaatcgat tggttttcat gataattaac taatatagtt
     1621 atatggtttt gtgcgctacc attaattatt cttttctcta ttcttctctt ctacaacttt
     1681 ttgaattgct gctcgatact tatcagggaa ctcttttacc gcctttaaaa gaccttcacc
     1741 acatggaggg cacagttcgt catcgtaaag taatccgcct tttgcgtagt cattacagaa
     1801 tatatttcgg catttataaa taatctctcc acaagttgta catttacatt tatctctgcg
     1861 gatagtgttt ttttctataa cttctggagc catttcgcta ttgcaaaaag ggcacttacc
     1921 tttgtgtttt ctttccatgg tatttttcta tttgtttatc tctttgcaat tgggattcct
     1981 gcttggaatc ccaacatgag aatcaattta tattagccgt tgtagccatg acgacgtgcg
     2041 ttatcaatac aatcagattt attgacatat ccttgagatg atgctccaac aattcgcccg
     2101 ttgcttgcag ttctgcgcca gcgccactct tttgaattat cgagataaaa ctcccactta
     2161 tcgttcattt aggtttcctt cttccagtta gatatttagt gatgatttgt accagcaggt
     2221 aggtatcatt tcattttttt ttctaataag cgtgttacaa ctgtactgac gacgatactt
     2281 aaggctgttg tccctaatgc gccgatactt ctacctatgt tcgacatgca tgacgggcat
     2341 aattcatcat catataaatg accgccctta gcgtaattct cacatcctgg agtacgacat
     2401 acgtaaatta ttgatttaca atcagaacac tcacacttat ctcgccgtat cttgttttct
     2461 tgaattacac gggcattgat ttccttttta caaaaaggac acactccttt aggttttaag
     2521 tccattaggg tttacctaat agcactacaa cttattaatt ttgaacgaca tttagggcaa
     2581 taatcaggtg tcccaaatcc agctctactg gtccattcgt ggcggcagtt taagcattta
     2641 gcccgataga tgacgatacc tgccataaaa atcccctttt tatcatacac taatgttgat
     2701 ttattatatt ctgaacataa taaatgtaaa gtatactatg tgataaacgt aatgcattga
     2761 ttttattgat attaaaaata tttattagag tgctaataat ggtatttttt ttgatttttt
     2821 tattaaaaac aatatgttgt atatgtgttg gtgtcgtttg attacgttca ataaatgcgt
     2881 gtatcaatat tagatataca aaaatgaagt tagaaggtgg ctgggagttt taggagattt
     2941 gccctccttc agtaaaaaga tcaaaggatc ctcttgagat cctttttttc tgcgcgtaat
     3001 ctgctgcctg taaacgaaaa aaccgcctgg ggaggcggtt tagtcgaagg ttaagtcagt
     3061 tggggactgc ttaaccgggt aactggcttg gtggagcaca gataccaaat actgtccttc
     3121 tagtgtagcc gcagttaggc caccacttca agaactctta atatctcaat ccaccttgtc
     3181 cagttaccag tggctgctgc cagtggcgtt aaggcgtgtc ttaccgggtt ggactcaaga
     3241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3301 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
     3361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3481 tttcgccacc tctgacttga gcgtcgattt ctgtgatgct cgtcaggggg gcggagccta
     3541 tggaaaaacg ccagcggcgt ggccttttcc gggttcttgt tttttgctca catgtttcct
     3601 ttggcgttat cccctgattc tgtggataac cgcattaccg tctttgagtg agctgatacc
     3661 gctcgccgca gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc
     3721 ctggacgcgc attttctcct tacgcatctg tgcggcattt cacacccggc atggggcact
     3781 tttcatacaa tctgcactga tgccacatag ttaagccagt atacactccg ctagcgctac
     3841 gtgactggtt cagggcttcg ccccgaaacc ccctgacgcg ccctgacggg cttgtctgct
     3901 cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgtatgt gtcagaggtt
     3961 ttcaccgtca tccccgaagc gtgcgaggca gctgcggtaa agctcgtcgg cgtggtcgtg
     4021 aagtgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagtt tctccagcag
     4081 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
     4141 cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg
     4201 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgctg
     4261 tgagggtaaa caactggcgg tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg
     4321 ttccgggaag ctgccaactt tggctccgcc gttactgcgt cagctggccg ccatcgggaa
     4381 taacctgaat cagacagcga ggaaggtgaa cagcgggcag tggtcttccg gcgaccgggt
     4441 tcaggtggtg gccgcactga tggccatcga gagagagttg cgcagcttac ggcaggtcgt
     4501 cagggaacag ggagcgcggg atgatagtta agtttcatgc caggggaaaa ggtggtggca
     4561 gtggtccggt tgattacctg ctggggaggg agcgtaatcg cgaaggcgca acggtgcttc
     4621 agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg aagaaataca
     4681 cgtccggtgt tctgtcgttt gcggagaaag aactgccacc gggaggacgt gaaaaagtga
     4741 tggcgagttt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt
     4801 gggtggagca ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc
     4861 tacagaccgg aaaacgcctc cagccgtact acgaccgggc agacaggccg agaattgatg
     4921 cctggcagac gctggt
//
