LOCUS       NZ_CP148456             5631 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0009 plasmid pZ1323CEC0009-4,
            complete sequence.
ACCESSION   NZ_CP148456
VERSION     NZ_CP148456.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441576
            Assembly: GCF_037573545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 107X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037573545.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:20:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,781
            CDSs (total)                      :: 5,650
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 330
            CDSs (without protein)            :: 330
            Pseudo Genes (ambiguous residues) :: 0 of 330
            Pseudo Genes (frameshifted)       :: 117 of 330
            Pseudo Genes (incomplete)         :: 210 of 330
            Pseudo Genes (internal stop)      :: 51 of 330
            Pseudo Genes (multiple problems)  :: 46 of 330
            Pseudo Genes (short protein)      :: 4 of 330
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148456.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0009"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0009-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-27"
     gene            273..803
                     /locus_tag="WHU68_RS28790"
                     /old_locus_tag="WHU68_28790"
     CDS             273..803
                     /locus_tag="WHU68_RS28790"
                     /old_locus_tag="WHU68_28790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            810..1043
                     /locus_tag="WHU68_RS28795"
                     /old_locus_tag="WHU68_28795"
     CDS             810..1043
                     /locus_tag="WHU68_RS28795"
                     /old_locus_tag="WHU68_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1524..2576
                     /locus_tag="WHU68_RS28800"
                     /old_locus_tag="WHU68_28800"
     CDS             1524..2576
                     /locus_tag="WHU68_RS28800"
                     /old_locus_tag="WHU68_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            2578..3444
                     /locus_tag="WHU68_RS28805"
                     /old_locus_tag="WHU68_28805"
     CDS             2578..3444
                     /locus_tag="WHU68_RS28805"
                     /old_locus_tag="WHU68_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            4304..4624
                     /locus_tag="WHU68_RS28810"
                     /old_locus_tag="WHU68_28810"
     CDS             4304..4624
                     /locus_tag="WHU68_RS28810"
                     /old_locus_tag="WHU68_28810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_187655770.1"
                     /translation="MDNRVSVFEKGESARRSRTTERSESVSEEAEERLNWHFLLTHLCG
                     ISHRTWRTFHTIRTDAEWLSQYTLRYRYVTGSGLRPDTPKVNGLTRPGGLVCSRHPLTD
                     KL"
                     /name="hypothetical protein"
     gene            complement(4647..4838)
                     /locus_tag="WHU68_RS28815"
                     /old_locus_tag="WHU68_28815"
     CDS             complement(4647..4838)
                     /locus_tag="WHU68_RS28815"
                     /old_locus_tag="WHU68_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4870..5226
                     /locus_tag="WHU68_RS28820"
                     /old_locus_tag="WHU68_28820"
     CDS             4870..5226
                     /locus_tag="WHU68_RS28820"
                     /old_locus_tag="WHU68_28820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000538661.1"
                     /translation="MGVMIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGE
                     PVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRR
                     LRLAVREQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4472..4553
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          3828..4015
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          3..1147
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5217..5629
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="9.91e-84"
ORIGIN
        1 gaatcaccat tacggtctgc atgaccccaa cgccccggag aaccgccgga cgctgaccct
       61 tcctgataac ctgccggaaa cgaaacaggc gcttgctgag ggcgtcacac gcgggataga
      121 cgcgctttac catgccggag agataaaagg ccgtcaggat gtgattcagg cgctcacaga
      181 ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag acccgaacgg
      241 cgggaggaat atcagactga aaggagcgtt ttatgagcaa tcttttacag acgggcgcgg
      301 agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg aacgacgagt
      361 tcaggaagct cgaagaatct gtaaacaggg aactgacctc aaacgaatct ttaatcagga
      421 acgctatcaa cgaccataca acagccctga aagaactgct ggagaggtat cagaaaacga
      481 ccgtcgatac gatggacgcg cactggaaaa ccgtactgaa aatgagcgtg aaacgctggc
      541 tgtggctgat tatcgtctca gtactgatgt ttgccacgac cgggagtctg ctgtggtacc
      601 agggcatgaa gataaacgcg aatatgaata ttctccgcga gcagaaagag agtctggaga
      661 agctgaacgc gaagacatgg ggcgtgcgtt atcacgagga cagcaacgga cgttttctgg
      721 tgttgccgaa ggggatgaaa gccgaaacga actggacgaa ggacaacggc aaactgaacg
      781 cagtgagact ggtgcaggag tgacagagca tgacggagct ggaaagacat ttgctgaacg
      841 cattagagca actgcaacag gactatatgc agcggctgaa cgaatgggag aacgccttcg
      901 tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg aacgggcgag
      961 tcatgcagtt gagtcagcag gtgcagcact tgagcgagcg gacagaacgc ttgagccagt
     1021 tatacagcga gaacaggaga taagggatga acggctgatg caggaacgcg aacatgagtt
     1081 atctctggag cgggagcgtc agccggaaat ccaggagcgg acactggatg gcccttcgct
     1141 gggatggtga aaccatgaaa aatgacgtgt tcagtggatt aaggcgggct aatgtggcct
     1201 gtagcatctg gttgcatagg tattcatgcg gttaaaattt atcaggtgcg atcgcggcag
     1261 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgca ccgaacgcgt
     1321 tttggcggtg cgtacaatgc gacgttatgg taaatggagc ggattttcag gaaggagtga
     1381 tactaacaac tcgaacactg tgcaaataat gattttttta aaatatcggt aacgataata
     1441 aattatttcg ttttacgccg gtaataaata taatgtagtt tcagtatata aaaaaatata
     1501 tgaggtgtct tgtgggagct ttcatgaatc ttcaacaacg tataaataaa ctaccacaat
     1561 tatcatccag tttttcattt ggaaaagata ttgataatat tcattcattt atctttaacg
     1621 aaacttctaa agataaaatt gaagatttac taagaaaatg ggtgtcaggc aatcagccct
     1681 gtgtatttgg taaattggca agcaagaaaa ttaaagggct tgatttccat ttatctattg
     1741 taaatagccc ccaactatat aatgatgatg gtcatttgtt tgattttttg cgtaatgaaa
     1801 gagttcgttt taaagaacgc gcaagaagag gagaggtcag tgcgcatctc atttatttta
     1861 ttcatccaca acttgccttt gctcgtccat cagaagagtt agtggacata caaaaatata
     1921 tttgttcgct gcatatgcca gagtgctatc caataaaaga ggatgttatt tatactgaaa
     1981 gcgtaccatt tcaggataaa gatgggctaa aaatatataa ggctggtgtt aatgtttttt
     2041 actcttctgc tcatagaaca agaaatcatg atagaagaat accaggggga atacttattt
     2101 ctgtaaatgc cccggggcat tttatgaggc tggcgataga aaaaggtttc tataaagatc
     2161 aagaacaagc gttagcggat attcgtaaca tgactattca gtctgttggt aagggtgggt
     2221 attcacatcc ggaagggatt tctacaacat ggcattcaga atctaatttg gatcgttttg
     2281 gatgcccagt gcacaccggt aatagttctt attacagcgg tttttatcat actgatgtat
     2341 taattccagg agaattaact aaggatgaaa gattgctcca tgaaatagaa aatagtgatc
     2401 caatgatctt caactggaat gtattgtttt atgtttcatt agaagagttt cctatagatg
     2461 atccttatta tggagagttt attggtattc ctgtagatga tgcatctatg ttttttaact
     2521 cctttcaacc aaggaaattt gaaaacaaac ccctttatga gaaagaggat gattaatatg
     2581 attaaaggaa tgctttttgc ttttttagca gcgttctcat ggggggcagc tatcgtaatg
     2641 agcaaaaaag gagttgaaga actagatgcc ggaggggtgt ttttctggca gatttgtagt
     2701 gcagtaatac tatcttggat agttcttttt ataaaaagaa aaaaactccc attaaactca
     2761 aaagcgatac ttgcttatgc gacaggcgtt tttgaaccat tcttagcgta tacttttaca
     2821 ctctacggac tttattttat ttcagcagga agcgcatcaa ttattttttc acttgaatca
     2881 gtgtttatat tggtgttgtc tgttcttatt tgctcagtta aaattaattc cccgcaatat
     2941 tttatattgt tccttatagg tgcaattgtt ggcagtattt tggtagcctt gccagacatc
     3001 agtaatggat cgaataatat tgttggatat tgtcttgttg ttgccggtgt gttatcggca
     3061 gcattttatg ttgttatatc gtctcgcctt attaaatcgt ttgagcctat cacattattg
     3121 agcggacagt tgacattctc ccttatactg tcatcgctct ttatttttat atctaaatca
     3181 tcaatttatc ttccgttgga atccgtgatt attgtttttt catctggaat attacaatat
     3241 tttttagctt tttgttttta cttgaactcc ttgcgttggc tcccagttca tttggcaggg
     3301 ataatgttgt actttattcc gctagttgca ttatttttgt catggttgtt tttggatgaa
     3361 caattcacat tcttacaggg ggtaggattt attgtaactg tgctaagtgt atattttctt
     3421 aacagaaaat actcagagga gtaacattct gttcaggatt gccccccggc ctccatcgat
     3481 accgtgccaa atgcacgtaa aaaacgggag cgtttctggt gtcggaatgt aaccaagggg
     3541 gtcagaggcg cgctaaaacg ctctcagggg agtcaaatag agacgtttgt aacaaaaaga
     3601 tcaaaggatc ctcttgagat cctttttttc tgcgcgtaat ctgctgcctg taaacgaaaa
     3661 aaccgccttg cagggcggtt tgatcgaagg ttaagtcagc ttgcagactg cttaaccagg
     3721 taactgtctt atgcgagacg agtcaccaaa attgttcttc tagtgtagcc gcactccggc
     3781 agccacttca agtactctct aaatatctcg cacatgactt ttaccaatgg ctgctgccag
     3841 tggcgctttg tggtgtcttt tccgggttgg acccaagaga acagttaccg gagaaggcgc
     3901 agcagtcggg ctgaacgggg ggttcctgca tacagcccag cccggagcga actgcctaag
     3961 tcgccccgag ataccagaag acgtgagcta tgagaaagcg ccacgtgtcc cgtaagggag
     4021 aaaggcggaa aaggtatccg gtaatcgata tggcgggaac gtgggggcgc aggagggagc
     4081 gattcaccgg aaacggtgat gatctttaag ccacgtcggg tttcgccacc gctggcggat
     4141 ttcatggttg agccgcaagg ctcccacaga tgcaccgaaa aagtgtctgt tttgtgaagt
     4201 ccgtcagggg ggcggagcct atggaaaaag cgcctccggc gcggcctttc ccggggtttc
     4261 gttttttgcg cacatgttct ttcctggttt atccccgggt tctgtggata acagggttag
     4321 cgtttttgag aaaggagagt ccgctcgccg cagccgaacg accgagcgta gcgagtcagt
     4381 gagcgaggaa gcggaagagc gcctgaactg gcattttctc cttacgcatc tgtgcggcat
     4441 ttcacaccgg acatggcgca cttttcatac aatccgcact gatgccgaat ggttaagcca
     4501 gtatacactc cgctatcgct acgtgactgg gtcagggctt cgccccgaca ccccaaaggt
     4561 caacggcctg acgcgccctg gcgggcttgt ctgctcccgg catccgctca cagacaagct
     4621 gtgaccgtct ccgggagctg catgtgtcag cggttttcac cgtcatcccc gaagcgtgcg
     4681 aggcaactgc ggtaaagctc atcggcgtgg tcgtgaagcg attcacagat gtctgcctgt
     4741 tcatccgcgt ccagttcgtt gagcttctcc agcagcgtta atgtctggcc tctgatgaag
     4801 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaagggggg
     4861 gttctgttca tgggggtaat gataccgatg aaacgcgaga ggatgctcac gatacgggtt
     4921 actgatgatg aacatgcccg gttactggaa cgctgtgagg gtaaacaact ggcggtatgg
     4981 atgcggcggg tctgcctggg tgagccggtt gcccgttccg ggaagctgcc gacactggca
     5041 ccgccgttac tgcgtcagct ggccgccatc gggaataacc ttaaccagac agcgaggaag
     5101 gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg tggtggccgc actgatggcc
     5161 atcggggatg agctgcgccg gctgcgtctg gcggtcaggg aacagggggc gcgggatgat
     5221 agttaagttt catgccaggg gaaaaggtgg tggcagtggt ccggttgatt acctgctggg
     5281 aagggagcgt aaccgggaag gggcaacggt gcttcagggg aatccggaag aggtccggga
     5341 actcatcgat gccacgccat ttgcgaagaa atacacgtca ggtgttctgt cgttcgcgga
     5401 gaaggagctg ccgcctggag ggcgtgaaaa agtgatggcg agctttgagc gtgtactgat
     5461 gcccggtctc gagaaaaatc agtacagcat cctgtgggtg gagcaccagg acaagggacg
     5521 gcttgagctg aattttgtca ttccgaacat ggagctacag accggaaaac gcctccagcc
     5581 gtactacgac cgggcagaca ggccgagaat tgatgcctgg cagacgctgg t
//
