LOCUS       NZ_CP148413             3372 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323CEC0017 plasmid pZ1323CEC0017-5,
            complete sequence.
ACCESSION   NZ_CP148413
VERSION     NZ_CP148413.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441584
            Assembly: GCF_037561805.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037561805.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:14:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,463
            CDSs (total)                      :: 5,336
            Genes (coding)                    :: 4,993
            CDSs (with protein)               :: 4,993
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 343
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 343
            Pseudo Genes (frameshifted)       :: 112 of 343
            Pseudo Genes (incomplete)         :: 243 of 343
            Pseudo Genes (internal stop)      :: 51 of 343
            Pseudo Genes (multiple problems)  :: 59 of 343
            Pseudo Genes (short protein)      :: 4 of 343
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148413.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323CEC0017"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323CEC0017-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-04-19"
     gene            complement(870..1103)
                     /locus_tag="WHU09_RS27255"
                     /old_locus_tag="WHU09_27255"
     CDS             complement(870..1103)
                     /locus_tag="WHU09_RS27255"
                     /old_locus_tag="WHU09_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1110..1670)
                     /locus_tag="WHU09_RS27260"
                     /old_locus_tag="WHU09_27260"
     CDS             complement(1110..1670)
                     /locus_tag="WHU09_RS27260"
                     /old_locus_tag="WHU09_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032329292.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISGHTTSVKQQLDTLSATVSTQFSTTEAELSRQQKKLLWQVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1702..2358)
                     /locus_tag="WHU09_RS27265"
                     /old_locus_tag="WHU09_27265"
                     /pseudo=""
     CDS             complement(<1702..2358)
                     /locus_tag="WHU09_RS27265"
                     /old_locus_tag="WHU09_27265"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2348..2671)
                     /locus_tag="WHU09_RS27270"
                     /old_locus_tag="WHU09_27270"
     CDS             complement(2348..2671)
                     /locus_tag="WHU09_RS27270"
                     /old_locus_tag="WHU09_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2735..2926
                     /locus_tag="WHU09_RS27275"
                     /old_locus_tag="WHU09_27275"
     CDS             2735..2926
                     /locus_tag="WHU09_RS27275"
                     /old_locus_tag="WHU09_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3008..3088)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(788..2358)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.817"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtgaaaggt tggagggaac ggcaacaact cacccctccc agtgaaggga cgggtagttt
       61 gccgctctcg atatgccgga cggagccaag gccatcgatc acggggcgcg tcagtggaag
      121 aacccgcgcc tgcgtgacgt atgcgtcttc gccgcaccat ctcacccgtc acgccaccac
      181 ataccgggaa ctgccgccaa ggtctaactc aaccaatcta gccgcgctac atacggtttt
      241 tgtggcaact catcaggtgt ccgctgtcga tgaaactctc tgcgccggta gcggaactct
      301 cacgcgtgcg gtcgccgtcg tcacagctcc cccaaccgca aacgattctc gcaaatacgc
      361 aagtatctgc aaaaacaggt ttttttcggg gttcctggga gactgtgctg ttgaaaaatt
      421 cttgaagaat acagaaggct catgcaaact gaatgaactg gtttctgttg tggatctcaa
      481 cagtttccac aggaacattg aggaggtcag agacctcaat gaacccgaaa cagctcgcct
      541 tcctggcgag ctgttttcgt tctacgcccg ccgacgaaag atcgcaagtg caataacgag
      601 atttttcacg tctttacgct caaaaagtaa aaaaaccatg ttttcccctg gctgaatccc
      661 caaaaacgcg tgaaatgcgt tttcaggatg ttgcatagct atgcacaaat acggcacacg
      721 atttgtttcg ctcgccccag ccgtcgaact ggtcaaaatg gcaagatttc gtcacttccc
      781 cggtcacatc tccattgatg gcccactccg aacccgtgcc gcgcgttcct gtacctcatg
      841 tctgtaccgg gcacgctcct gttcatccct tagctgttgt tcgcgataaa taaccgactc
      901 aagcgatcca cttgcccggc taatctctgc acctgctgac tcaatatcac gacgcgctcg
      961 ttcagtatcg cgttgttccg ttgcgtaacc tcgtgcatct tctgcaattc cgcgaaggcg
     1021 ctctcccact cgttcagccg ccgcatatag tcctgttgca aatgctctaa ggcgttcagc
     1081 aaatgccctt ccagttccgt catactcgtt tactccctga ccagcctcac ggcgttcttg
     1141 tcgcccaccg tccaggtgtt ttcccctttc accccgtccg gcaacaccag aaatttcccg
     1201 ttctcgccat tgacgaacgt caccccccag gtttttgcct cgagtttcgc cagcgtgttt
     1261 tcctgctctc tgatagtcag gatatttttc gcaatcttgc tttcttgcca ctccatcagc
     1321 ccccagcacc ccaggaaaat gccgcccgtg acgctgagcg cggtcagcgc cggaaacagt
     1381 atccgccctt tgatcacctg ccagagcagt tttttctgct gccgggacag ttcggcctct
     1441 gtggtgctga actgcgtgct cacggtggcg ctcagtgtat ccagttgctg tttcacggat
     1501 gttgtatgtc cgctgatagc gtctctgatt ttctgcctgt ttaagctcag tgccttgtct
     1561 acagactctt cgagcttcct gaactcgctg ttcagcatgt tctcggtaga agcggctcgc
     1621 tctttcagtt tcttctcgaa gtctgttccc atttgtaaaa gattgctcat acagcgcccc
     1681 ctttagcctg agattccgcc caccctccgg gtcggcaata ctgatactgc ttctggttgt
     1741 cctcaccacc tcaaaacctg ccgctgtgag cgcctcagtg acatcctgac gcgtttttat
     1801 tgctccggca tggtaaagcg cctcaagacc ccgtgtaatc gcttctgcgg cctcctgttt
     1861 cgcttttggc agattatttg gcgtgacaag cgtccgtctg ttctccggtg cgttcgggtc
     1921 acgcagcccg taatgatgat taaccagcgt ctgccaggca tttatcctcg gcctgtccgc
     1981 acggtcgtag tacggctgga ggcgcttacc gctctgtagc tccatgttcg ggatgacaaa
     2041 attcagttcg agccgcccct tatcctggtg ttccacccag agaatgctgt actggtcttt
     2101 ttcgagaccg ggcataagaa cacgctcaaa gctcgtcatc actctttcgc gttcacccgg
     2161 cggtaactcc ttctccgcga acgacagaac gcctgacgtg tatttcttcg aaaatggcgt
     2221 ggaatcaatg agttcccgca cttcttccgg gttaccccgg agcaccgttg cgccttcgcg
     2281 gttacgctcc cgccccagca ggtaatcgac cgggccactt ccaccgcctt tccccctggc
     2341 atgaaattta actatcatcc cgtaccccct gttccctgac agcctgccgc aactggcgca
     2401 attcaccctc gatggccatc agtgcggcca ccacatgaac ccggtcaatc gaagaccact
     2461 ggccgctgtt caccttccgg gctgtctggt tcaggttgtt cccgatggca gccagatgcc
     2521 gcagcaatgg cggcgatagt gtcggcagtt ttcctgtgcg tgcaaccggc tcccccagac
     2581 agacccgccg catccattcc gccagtcgtt taccctcgca gcgttccagt aaccgggcgt
     2641 gctcctcatc ggtaacccgt atcgtgagca tcttctcgcg tttcatcggt atcattcccc
     2701 ctaacaaatc ccccatacaa ggagccttaa gaccatgacc aaacaggaaa aaacagccct
     2761 taacatggct cgctttatca gaagccagac gctaaccctg ctggaaaaac tcaacgatct
     2821 ggacgcggac gaacaggcag acatctgcga atcacttcac gaccacgccg acgaactcta
     2881 ccgcagttgc ctcgcacgct tcggcgatga cggtgaaagc aactgaaaca tgcagctccc
     2941 ggagacgttc acagcttgcc tgtgagcgga tgccgggagc agacaagccc gccagggcgc
     3001 gtcagtgcgg gtgtcggggc gcagccctga cccagttcac agagcgatag cgatgtgtat
     3061 actggcttat ctcattttgc atcggtgcag acatggatgg gaagtgcacc atgtcaggag
     3121 taaaaagccg cacagatgta aaaacagcgc acccgataaa cagcacacac gcggacactg
     3181 gcacaggcgc gtaagtgcac aatgcttgca tctgtgactc ttccgcttcc tcgcccactc
     3241 gctcactacg ttcggtcgct ggggctgcgg cgagcggtgg gatcactctc gttgtgatgc
     3301 agatccattt ttttttttgc actggagtta tcaggagttg cggtgcacat gggatcgtgc
     3361 gatatgccgg at
//
