LOCUS       NZ_CP148377             2689 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0108 plasmid pZ1323HEC0108-4,
            complete sequence.
ACCESSION   NZ_CP148377
VERSION     NZ_CP148377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441593
            Assembly: GCF_037549885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037549885.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,364
            CDSs (total)                      :: 5,227
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 137
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 100 of 249
            Pseudo Genes (incomplete)         :: 165 of 249
            Pseudo Genes (internal stop)      :: 43 of 249
            Pseudo Genes (multiple problems)  :: 54 of 249
            Pseudo Genes (short protein)      :: 2 of 249
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0108"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0108-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-04-27"
     gene            complement(join(2575..2689,1..80))
                     /locus_tag="WHU33_RS26805"
                     /old_locus_tag="WHU33_26805"
     CDS             complement(join(2575..2689,1..80))
                     /locus_tag="WHU33_RS26805"
                     /old_locus_tag="WHU33_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_039059175.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEIA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            365..688
                     /gene="mobA"
                     /locus_tag="WHU33_RS26810"
                     /old_locus_tag="WHU33_26810"
     CDS             365..688
                     /gene="mobA"
                     /locus_tag="WHU33_RS26810"
                     /old_locus_tag="WHU33_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_004988908.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(914..1639)
                     /locus_tag="WHU33_RS26815"
                     /old_locus_tag="WHU33_26815"
     CDS             complement(914..1639)
                     /locus_tag="WHU33_RS26815"
                     /old_locus_tag="WHU33_26815"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="WP_005112197.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLFQ
                     KDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDF
                     TKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKE
                     YATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
                     /name="putative adhesin"
     gene            complement(1916..2077)
                     /locus_tag="WHU33_RS26820"
                     /old_locus_tag="WHU33_26820"
     CDS             complement(1916..2077)
                     /locus_tag="WHU33_RS26820"
                     /old_locus_tag="WHU33_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007783.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     Typing          119..356
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.583"
                     /coverage="100.0"
                     /evalue="4.55e-120"
     Typing          complement(2006..2532)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.472"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          366..685
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="7.599999999999999e-62"
ORIGIN
        1 tccagctttt cgagcagcgt caggctctgg ctttttatga atcccgccat gttgagtacg
       61 gtttgctgct gcttgttcat ctttctgttt tctccgttct gtctgtcatc tgcgttgtgt
      121 gattatatcg cgtaccactt ttcgactgtt ttaccgccgg tattctgccg tctgacgcat
      181 tgacgggtat ttctgcctga caatactgtc actgccaaaa aactgccgtg cctttgtcgg
      241 taattcgaac ttgctgacag gacaggatgt gtaattgtta taccgcgcat acatgcacgc
      301 tattacaatt accctggtca gggcttcgcc ccgacacccc gtgtcagata cggagccatg
      361 ttttatgaca aaacgaagtg gaagtaatac gcgcaggcgg gctatcagtc gccctgttcg
      421 tctgacggca gaagaagacc aggaaatcag aaaaagggct gctgaatgcg gcaagaccgt
      481 ttctggtttt ttacgggcgg cagctctcgg taagaacgtt aactcactga ctgatgatcg
      541 ggtgctgaaa gaagttatga gactgggggc gttacagaaa aaactcttta tcgacggcaa
      601 gcgtatcggg gacagggagt atgcggaggt gctgatcgct attacggagt atcaccgtgc
      661 cctgttatcc aggcttatgg cagattagct tcccggggag aaaactgttg aaaacagacg
      721 gtatgaacac ggtaagctcc caaagtgatc tccattcgct ttcatgcata gctatgcagc
      781 gagctgaaaa cgatcctgac gcatccttcc tgtttttccg gggtaaaaca tctatttttg
      841 tggtgtctgc gtcagaatcg tgttcagcgc gtttcagtgg tgcgtacaat taagggatta
      901 tggtaaatga tgattatttc tcctctctat ttactcggca agcgccaaag tgaattacat
      961 catattttac tcctgattca tttattactt taaatatgtc agaaaggtgc ttcttatgat
     1021 ttttatcagt tataaatgct atatcaatat ttttgtttcc taagccatct tgatgtcttt
     1081 gtataatatt ttgggcttgt tcatggtaat atagagcata gttttttatc ttctcattac
     1141 ttgttgcgta ttcttttcta tatatggctt gtgacatcat ttcttcttct aattttgatt
     1201 gaggtatatt gcgcgcgttc gctagaactt ttaggtcatc tttgcttatg tttagtgatg
     1261 gtgttaaccc tttttgagct tcagtatggc gtttgtttac ctctgaaaga acgcttagtc
     1321 cctttgtgaa gtcgttgtct tttgtataaa aatcaattct taggtttttg gggactacag
     1381 tccatccatc gcctttttga cgcgtgaata gtgtttgtgg atgggagatc tctttccatc
     1441 caccatgcga cataatgaca agttgtgttg cttttcctgt ttggctcaat cctattccgt
     1501 ctttctggaa taaacgacga tgacttgtta cgtccatgtt tggtgatggg tgtgttttta
     1561 tatttttgga tgatgttaga aggtgatttc ttattgttga tgatatgttt gttatgagat
     1621 tcgaattgag tattttcatt tgttaacaat ccatttttgc ggtgtttttt attgtttttt
     1681 ttcttttgtt taagggcgtg tcggtgtaag tgtttatttt atgggttaaa gtaggatacc
     1741 aagtgtgaaa aaaaattaaa ggttcttctt gagatccttt ttttctgcgc gtaatctgct
     1801 gcctgtaaac gaaaaaaccg ccctgcaagg cggtttttcg aaggttctca gagctaccaa
     1861 ctctttgagc cgaggtaact ggcttggagg agcgcagtca ccaaaacttg ttctttcagt
     1921 ttagccttaa caggcgcatg acttcaagtc ttactcctct aattcggtta tcagtggctg
     1981 ctgccagtgg cgataattcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2101 cctacaccgg gccgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag
     2161 ggagaaaggc ggacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcacgaggg
     2221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccacct
     2341 gcgcggcctt tgtccctgcc ttcggctttt tgctcacatg ttctttccgg cgttatcccc
     2401 tgattctgtg gataaccgca ttaccgcctt tgagtgagct gacaccgctc gccgcagtcg
     2461 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa gagcgcctta tgtgacattt
     2521 tctccttacg caccggtata ccgttctgca tcctgtctgg agcaggatac actgttatac
     2581 cccggtgcca ctttccgctt caaagcgtgt ctgtatgctg ttctggagtt cttccgcgat
     2641 ttcgtgcagc ttctcacaca tggcggcctg ttcgtcggca tcgagtgcg
//
