LOCUS       NZ_CP148370             2678 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0300 plasmid pZ1323HEC0300-5,
            complete sequence.
ACCESSION   NZ_CP148370
VERSION     NZ_CP148370.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441595
            Assembly: GCF_037546845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037546845.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:14:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,941
            CDSs (total)                      :: 4,828
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 81 of 178
            Pseudo Genes (incomplete)         :: 113 of 178
            Pseudo Genes (internal stop)      :: 40 of 178
            Pseudo Genes (multiple problems)  :: 49 of 178
            Pseudo Genes (short protein)      :: 4 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148370.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0300"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0300-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-06"
     gene            complement(join(2509..2678,1..154))
                     /gene="mobA"
                     /locus_tag="WHU13_RS24690"
                     /old_locus_tag="WHU13_24695"
     CDS             complement(join(2509..2678,1..154))
                     /gene="mobA"
                     /locus_tag="WHU13_RS24690"
                     /old_locus_tag="WHU13_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            438..632
                     /locus_tag="WHU13_RS24695"
                     /old_locus_tag="WHU13_24700"
     CDS             438..632
                     /locus_tag="WHU13_RS24695"
                     /old_locus_tag="WHU13_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041639.1"
                     /translation="MNKQQQTVLNMAGFIKGQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1120..1278
                     /locus_tag="WHU13_RS24700"
                     /old_locus_tag="WHU13_24705"
     CDS             1120..1278
                     /locus_tag="WHU13_RS24700"
                     /old_locus_tag="WHU13_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005093880.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     gene            1558..2283
                     /locus_tag="WHU13_RS24705"
                     /old_locus_tag="WHU13_24710"
     CDS             1558..2283
                     /locus_tag="WHU13_RS24705"
                     /old_locus_tag="WHU13_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="WP_032202691.1"
                     /translation="MKIFNLNLIANISSTIRTHLPTLQKNIRPHPSPNLDVTSHHRVFQ
                     KDGIGLSQTGKSTQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPQNLRLDFYTKDNDF
                     TKGLSVLSEVNKRHTEAQTGLTLSLNISKDDLSFLAKARNIPQSKLEEEMKSQAIYRKE
                     CATGNEKIKNYALYYHDQAQNIIQRHQDGSGNQNIDIAFITDKNHKKHLSDIFKVINDS
                     GVQYDVIHFGACRVNRDEQ"
                     /name="putative adhesin"
     Typing          complement(164..400)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.745"
                     /coverage="99.0"
                     /evalue="3.58e-116"
     Typing          662..1192
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.327"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(3..154)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.31e-24"
     Typing          complement(2513..2676)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="99.0"
                     /evalue="1.49e-26"
ORIGIN
        1 ctttcttacc gagagctgcc gcccgtaaaa aaccggaaac ggtcttgccg cattcagcag
       61 ccctttttct gatttcctgg tcttcttctg ccgtcagacg aacagggcga ctgatagccc
      121 gcctgcgcgt attacttcca cttcgttttg tcataaaaca tggctccgta tctgacatgg
      181 gtgtcggggc aaagccctga ccagggtaat tgtaatagcg tgcatgcatg cgcggtataa
      241 caattgcaca tcctgtcctg tcagcaagct cgaattaccg acaaaggcac ggcagttttt
      301 tggcagtgac agtattgtca ggcagaaata cccgtcaaag cgtcagacgg cagaataccg
      361 gcggtaaaac agtcgaaaag tggtacgcga tataatcaca taacgcagat gacagacaga
      421 acggagaaaa cgaaaagatg aacaagcagc agcaaaccgt actcaacatg gcgggattca
      481 taaaaggcca gagcctgacg ctgctcgaaa aactggacgc actcgatgct gacgagcagg
      541 ccaccatgtg tgagaagctg cacgaactcg cagaagaact ccagaacagc atacagacac
      601 gctttgaagt agaaaatagc acagagagat aacgctcagg acaggatgcc gaacggcaca
      661 acagagcgta aggagaaaat gtcacataag gcgctcttcc gcttcctcgc tcactgactc
      721 gctacgctcg gtcgttcgac tgcggcgagc ggtgtcagct cactcaaagg cggtaatgcg
      781 gttatccaca gaatcagggg ataacgccgg aaagaacatg tgagcaaaaa gccgaaggca
      841 gggacaaagg ccgcgcaggt ggcatttttc cataggctcc gcccccctga cgagcatcac
      901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat
     1081 ctcggcccgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1201 ttaacgccac tggcagcagc cattggtaac tggacaaggt ggattgagat attaagagtt
     1261 cttgaagtgg tggcctaact gcggctacac tagaaggaca gtatttggta tctgtgctcc
     1321 accaagccag ttacccggtt aagcagtccc caactgactt aaccttcgac taaaccgcct
     1381 ccccaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag
     1441 aagaaccttt aatttttttt cacacttggt atcctacttt aacccataaa ataaatactt
     1501 acaccgacac gcccttaaac aaaagaaaaa aaacaccgca aaatggattg ttaataaatg
     1561 aaaatattta atttaaattt aatagcaaac atatcatcaa caataagaac ccacctcccg
     1621 acactacaaa aaaatataag accacatcca tcaccaaacc tcgacgtaac aagtcatcat
     1681 cgtgtattcc agaaagacgg aataggattg agccaaacag gaaaatcaac acaacttgtt
     1741 attatgtcgc acggtggatg gaaagagatc tcccatccac aaacactatt cacacgtcaa
     1801 aaaggcgatg gatggactgt agttccccaa aacctaaggc ttgactttta tactaaagac
     1861 aacgacttca caaagggact aagcgttctt tcagaggtaa acaaacgcca tactgaagca
     1921 caaacagggc taacactatc actaaacata agcaaagatg acctaagctt tctggcgaaa
     1981 gcgcgcaata tacctcaatc aaaattagaa gaagaaatga aatcacaagc tatatataga
     2041 aaagaatgcg caacaggtaa tgagaagata aaaaattatg ctctatatta tcatgaccaa
     2101 gctcaaaaca ttatacaaag acatcaagat ggttcaggaa atcaaaacat tgacatagca
     2161 tttataactg ataaaaatca taaaaaacac ctttccgata tatttaaagt aataaatgat
     2221 tcaggagtac aatatgatgt aattcatttt ggtgcttgtc gagtaaatag ggatgagcaa
     2281 taatcatcat ttaccataat cccttaattg tacgcaccac tgaaacgcgc tgaacgcgat
     2341 tctgacgcag acaccgcaaa aagagatgtt ttaccccgga aaaacaggaa ggatgcgtca
     2401 ggatcgtttt cagctcgctg catagctatg catgaaagcg aatggtgatc actttgggag
     2461 cttacggtgt tcataccgtc tgttttcgac agttttctct ccgggaagct aatctgccat
     2521 aagcctggat aacagggcac ggtgatactc cgtaatagcg atcagcacct ccgcatattc
     2581 cctgtccccg acacgcttgc cgtcgataaa gagttttttc tgtaacgccc ccagtctcat
     2641 aacttctttc agtacccgat catcagtcag tgagttaa
//
