LOCUS       NZ_CP148369             4072 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0300 plasmid pZ1323HEC0300-4,
            complete sequence.
ACCESSION   NZ_CP148369
VERSION     NZ_CP148369.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441595
            Assembly: GCF_037546845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037546845.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:14:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,941
            CDSs (total)                      :: 4,828
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 81 of 178
            Pseudo Genes (incomplete)         :: 113 of 178
            Pseudo Genes (internal stop)      :: 40 of 178
            Pseudo Genes (multiple problems)  :: 49 of 178
            Pseudo Genes (short protein)      :: 4 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148369.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0300"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0300-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-06"
     gene            complement(405..659)
                     /gene="mobC"
                     /locus_tag="WHU13_RS24675"
                     /old_locus_tag="WHU13_24680"
     CDS             complement(405..659)
                     /gene="mobC"
                     /locus_tag="WHU13_RS24675"
                     /old_locus_tag="WHU13_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            838..2997
                     /gene="mobQ"
                     /locus_tag="WHU13_RS24680"
                     /old_locus_tag="WHU13_24685"
     CDS             838..2997
                     /gene="mobQ"
                     /locus_tag="WHU13_RS24680"
                     /old_locus_tag="WHU13_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3253..3945)
                     /locus_tag="WHU13_RS24685"
                     /old_locus_tag="WHU13_24690"
     CDS             complement(3253..3945)
                     /locus_tag="WHU13_RS24685"
                     /old_locus_tag="WHU13_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(661..837)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          838..1196
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(3254..3945)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          752..2994
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcctccacct aaacacctta aattcctcca cctttacctt aaattcctcc acatatacct
       61 ctataacata ttgattttat taatgaattt tgaccctaaa acaagtatta aaaacaagat
      121 cttaaaaaca agatctaaaa acttaacggc gagcctgtgg ataacttggg gcgctgaaaa
      181 caaaaaacca ataaaaacaa tgtgttgcaa gaaagcagaa ttgcaagttc cgaatactcc
      241 ccttccaacc ctgtggaaat ccagtattca tgcgggttca gcgcatatca cctaccgaaa
      301 aaccgtgcgt atgtgctcaa cctggcgacg gattcacttc cgcttcctcg cccactcgct
      361 cgctaacgct cggtcgctgg ggctgcggcg agcggacgat acaatcactg tcctggcagc
      421 gcctccagct cgttaatcag cggcagtatg cgcgtcacat cagcctgtcg tgtcacctct
      481 gccttcagga actggagtaa ccaggcacgc aactgcacct cctccggctt tctgaccttt
      541 gccagcaatg ctgcacccac aacaaccttc tgcgctgtgt ccaggctccg gctcttcgcc
      601 ttcaggcgct gtaatctcgc ctcagcatca gcaatcttct gttcgagcgt tctgctcatg
      661 atgtaactcc gtgcgcgatg aaaaatcgca ttttagcgcg tcactggtag tttaaaaact
      721 gagctggcat aatgcgcgac aaatcacaaa gtgcgcactt atacaatctc cactccgttt
      781 cgattgtgtg cgccctgcgg ggctgaaaga aaactacaaa aaggcattac ggcagaaatg
      841 gcgatttatc atctcagcat gaaaattatc tccagaagca gcggctacag cgccgtagct
      901 tccgctgcct atcgttccgg ttcactgatg cttgatgagc gtaccggact gactcacgac
      961 tacacccgca aaagcggtgt ggctgaagct gtgattctga cccccgcgac agcccctgcc
     1021 tggtgtacga accgtgccga actctggaat gccgttgaga aggccgaatg ccggaagaac
     1081 tcacaacttg cgcgggaaat tgaactcgct atcccccgtg aactgccgca ggatgccgca
     1141 cgggaaaccg ttctcgcctt tgtcagagaa aactttgtca gtcagggcat gattgctgat
     1201 gtggcgttcc atcacatgga taaaacaaat ccccatgcgc acatcatgct gaccacgagg
     1261 gctgtcggac ctgctgggtt cggcggaaag gtcagggact ggaacgaccg gacacatgcc
     1321 gagacgtggc gtgcatcatg ggctgaccat gcaaacagag cactggcgaa cgccggttat
     1381 caggaagaga tagaccatcg ttcatacgag cgacaggggc ttgagaaaac gcctggtatt
     1441 cacctcggaa agtctgcctg tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa
     1501 cagaatcgtc tgattaacag gcttaacctg gaaatacaga tttcccgcac tgaactcagg
     1561 aacagcgggc tgacagagcc tgcccgccgt gtggctgacc ttctgaatat cgtgcttcct
     1621 gacaacgcaa cttcgtacac actacgtgac atcatcgaag cgttgccgaa ggacagtaat
     1681 gcggcatgga aactcaccag taatgccatg tcgatgatgg cagatatgca ggcgacgcgc
     1741 agacgctggg aagagctgaa caccgaacga aaggaagcga tgagccatgc tgccagcctg
     1801 aaaaaatcat cgccgttttc gtccggtttt tcccgtattc ccctgatgca atgggcggca
     1861 ccggaatacc gtagcgaaca gggaaaaatc cagtcgctga gccagcaaat ggaaaaattg
     1921 cgacagcatt accggaaggt ggagaaacag gacattcccg cgagtcagac ggcatttgaa
     1981 aaacagtggc agcagtgggg ggcatccggc cttgcagctc tcagagtcca gttgcttaaa
     2041 cgtgaagagg aattgcgccg gaaggagcag gaggatacag aacgtagtcg cgcggagcga
     2101 cgagcggaac agctcaggaa aaatgacaat gccgttatgg attcaggggt actgagcgca
     2161 gtcgttacgg ggtatggcga ggcccccatg cctggtaacg gggaaatgac ctgttacctt
     2221 ctgcttcaca accggaacgg agaatacacg ctctggggga acgagctgga gaaataccgc
     2281 accagaatct ttgagcgtgt tgatttaatg cgtgacagga gtggttatat ctgtgaccga
     2341 agcgaaatcg gtcaacatcc accactgcaa cgtgtttatt cctctgcgac ctttgagcaa
     2401 cttctgacgc aggtttgcca gacatggccg caatataccc gcaatctccg ccagccgaag
     2461 acatggccgg agtctttctg tcttggagaa gaccgccagc cagccatgcc atcactggca
     2521 gcaaggaagg tggattttac gcagggacgt ctgctaccga cgctgatgcc ggtaatgagt
     2581 tctgtcgacc gggaaacccg ccagcttcag cttttgctgg tcatgggcgt ggatgactct
     2641 ctgggcggtg ttgtccggct taatggtacg ctgtatcccg cgtttgccgt tccctcagcc
     2701 gataacagcc agctggtcat cagtgcactg accgataagg ggttacggta tgcagggtat
     2761 ggtgttgccg taaatcacga tgcagacagc cacatatcac ctgcgccgga acttatggag
     2821 tttcatctga aaacccgtga ggcaccgctt tttgctgccg tcaacacacc agaaaaacag
     2881 ccggatcatc tgttccgctc tctgggtttc aatcgtacct gggatgaatg gcggcgcgag
     2941 gaagatgcaa gaacacacac caccgagagg agacatgatc gcggttggtc acagtgaatg
     3001 tgaacaatat tttgttttgt ttaacatcgt gttatgttcg tcgatgttct tctatgttca
     3061 tcgacaggga tgaatgcaag aagcacacac ataatgcaag catagttttg tttttccgcc
     3121 cccaaaaatt gggggttctt ttttcgctgc attagagcgc ttttgacgca atttttcact
     3181 tcaatagtac cttcgcacta ctaacacaaa aaaaacgcag cagggagcgt tttaggtgcc
     3241 ttaaaatcga ttttagatcg tcatcttgat ctgtttgtct tccttgaagc gaaaatgcag
     3301 ggctacaacc gtgcgcccct ttttgatcgt ctcaaccgtt acagccaggt cgcttttttt
     3361 gttcagttcg tcaacacagg gttttattag tattttattt aagtctctga attgtttgta
     3421 tttattttca atccctaaca ttgaacgaaa gtcattaaga gcaataattc tctctcccgt
     3481 actgcggaat tgctggagga actcgtatat tcttatactg taactacttg ataaacctga
     3541 tatgtttttt ataacaacgc gcgtgaattg ccccttcagt tgcgtcagat aaggcattac
     3601 cgcgtcagaa aaggttattc gagccttggc ctcaccttta gcgtactgtg cccgatactg
     3661 gatccagcgg aactcctcac gcttctcgtc attcttgaga atgatcgacc tgtcccataa
     3721 acgatcgatc gcttcctgta actgaacctc ggcatttttc cgccccatgt cagggaatga
     3781 atcaaaatat tccgcagcgg taatggtcat tgtcttttgc aattcagcat cagcaccaga
     3841 tttcaatcga ctgatacaca gcaacagaaa acgttgttcc tgtagggtca acttgtagct
     3901 ggcctcaacc aaagcattcg acttgtaggc aatcaagtcc gacatacaaa gatcccgttc
     3961 aaatgtggtg atgctttaat gtgccccaca aataagaaat gtggaatatt tttttttata
     4021 tcctcacatt tctgtgaata acaccttaaa ttcctccacc tttaccttaa at
//
