LOCUS       NZ_CP148355            10681 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0399 plasmid pZ1323HEC0399-7,
            complete sequence.
ACCESSION   NZ_CP148355
VERSION     NZ_CP148355.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441599
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096685.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:27:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148355.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0399"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0399-7"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-17"
     gene            complement(388..807)
                     /locus_tag="WHU37_RS00005"
                     /old_locus_tag="WHU37_27855"
     CDS             complement(388..807)
                     /locus_tag="WHU37_RS00005"
                     /old_locus_tag="WHU37_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(888..1325)
                     /locus_tag="WHU37_RS00010"
                     /old_locus_tag="WHU37_27860"
     CDS             complement(888..1325)
                     /locus_tag="WHU37_RS00010"
                     /old_locus_tag="WHU37_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            1585..3150
                     /locus_tag="WHU37_RS00015"
                     /old_locus_tag="WHU37_27865"
     CDS             1585..3150
                     /locus_tag="WHU37_RS00015"
                     /old_locus_tag="WHU37_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(3147..3488)
                     /locus_tag="WHU37_RS00020"
                     /old_locus_tag="WHU37_27870"
     CDS             complement(3147..3488)
                     /locus_tag="WHU37_RS00020"
                     /old_locus_tag="WHU37_27870"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            3789..4412
                     /locus_tag="WHU37_RS00025"
                     /old_locus_tag="WHU37_27875"
     CDS             3789..4412
                     /locus_tag="WHU37_RS00025"
                     /old_locus_tag="WHU37_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_077150956.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRL"
                     /name="recombinase family protein"
     gene            4393..7359
                     /locus_tag="WHU37_RS00030"
                     /old_locus_tag="WHU37_27880"
     CDS             4393..7359
                     /locus_tag="WHU37_RS00030"
                     /old_locus_tag="WHU37_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_072024409.1"
                     /translation="MSFLDSKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFR
                     AFRPDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVH
                     LRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTL
                     NAKLKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLL
                     AEVHSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVM
                     MDRHLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKY
                     GKRPGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATE
                     TTFSMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLR
                     LKASIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHA
                     ILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDY
                     IRSQGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     gene            7368..7466
                     /locus_tag="WHU37_RS00035"
                     /old_locus_tag="WHU37_27885"
     CDS             7368..7466
                     /locus_tag="WHU37_RS00035"
                     /old_locus_tag="WHU37_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_225387326.1"
                     /translation="MAEMPTEPHIRDVQGGTVAPSSSSKLTGISVQ"
                     /name="colicin release lysis protein"
     gene            complement(8491..8682)
                     /locus_tag="WHU37_RS00040"
                     /old_locus_tag="WHU37_27890"
     CDS             complement(8491..8682)
                     /locus_tag="WHU37_RS00040"
                     /old_locus_tag="WHU37_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8747..9070
                     /locus_tag="WHU37_RS00045"
                     /old_locus_tag="WHU37_27895"
     CDS             8747..9070
                     /locus_tag="WHU37_RS00045"
                     /old_locus_tag="WHU37_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9279..>9710
                     /locus_tag="WHU37_RS00050"
                     /old_locus_tag="WHU37_27900"
                     /pseudo=""
     CDS             9279..>9710
                     /locus_tag="WHU37_RS00050"
                     /old_locus_tag="WHU37_27900"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9748..10266
                     /locus_tag="WHU37_RS00055"
                     /old_locus_tag="WHU37_27905"
     CDS             9748..10266
                     /locus_tag="WHU37_RS00055"
                     /old_locus_tag="WHU37_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001318237.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            10273..10506
                     /locus_tag="WHU37_RS00060"
                     /old_locus_tag="WHU37_27910"
     CDS             10273..10506
                     /locus_tag="WHU37_RS00060"
                     /old_locus_tag="WHU37_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          8319..8406
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(7750..8308)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9061..10610
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.031"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caccgggaac gcgcttcagt cataaacctt gctgaaaaca caggaacgcg ttaaaacgca
       61 tacgggcgcg attctggtgt gtctttcata gtcgtgtttt tgcctctatt gccagatcgc
      121 gctcattccc ctgaacttct gttttcctgc tcaaatcccc gtcttcttcg cttccgaaag
      181 tatgaaaaaa atccgtttca gcggtctctg actggcaatt cctgcgtgat tcacctggct
      241 gcataggtat gcatgtggtt aaaatttacc gggtgcgatc gcggcagttt tccgggtggt
      301 ttgttgccat ttttacctgt ctgctgccgt gatcgcaccg aacgcgtttc ggcggtgcgt
      361 acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac
      421 cgcagaaatc gtgtcagcca gcagcctgga ttttttcggg gtaaggtctt tgctgcggta
      481 atcagtaccc gcaaattgag ttgtgccccg gagtgagttt aaaaattcct ggctgtgaaa
      541 ctgttgaatg cgttgttgca catcactccg tgattttcct gtcgctttat ctttagctcc
      601 catatcccag aactgccgaa aaaaaggcac catcattcgt gttgtgtcgg ctttttgggt
      661 ggaaaagttg ggatcaaaac cgtcatcaga agcatatacg taatgccggg tatgttgttc
      721 cacgctgccc tttggggtac aggctgtgag agctactgtt gctgttatca tggcgataag
      781 acaaagtttg tttccggtac gtctcatgag tgtctcccta ccttaaagta tttattctcg
      841 gtgagttatt atccgaagat tatcttatca tcagtttctg agcgcgttca gtgaccgaac
      901 agcctggacc agaatccccg tttcttttcc tgctccagag cctgcctgag ctgggctatc
      961 tcattctgta gctgtttccg ttctgcttcc tggcgccggc gctccgtatc ctgggcttgt
     1021 ttatcctcaa gcatcagcgt caggcattgt ttcagctcat tcagttcccg gagaatgcgt
     1081 tctgtctgct gatcatgagg tttttctgag tgtccttgag tgtgcctttc tggtgtctca
     1141 ttccgcttta attcgccgta tgcccggatc agttcactgg tttcaaactc ccgtcgtccg
     1201 tcctggctgg tacggtaact gacaagacct gagcgcatat cacggtaaaa ctgtgaacgc
     1261 gatcttccag tcattttcag tgcgtcccgg agtgtgtgcc atgccataaa gttacagtgt
     1321 cccataggtg tctcatctca tggtttcagt aaaacataat gaggtctgag aacggtaatg
     1381 tttgtgctgg tttttgtggc atcgggtgga aatagcacgt ggtgtgggtg gctgtttttt
     1441 tgatcgtttt cacaaaaatg aaaatccaca gggttgacag ggaaaatgca gcggcgtaat
     1501 tttaaactgt atataaaacc agtgattata tatacagtat attgctttta actatttgtt
     1561 tttaatgtca aagaggattt tataatggaa acagctgtag cgtactataa agatggtgtt
     1621 ccttatgatg ataaggggca ggtaatcatt actcttttga atggtaatcc agacgggagt
     1681 ggctctggcg gtggtggtgg aactggaggt agcaaaagtg aaagttctgc agccattcat
     1741 gccacagcta aatggtctac tgctcaattg aagaaaacgc aggcagaaca ggctgcccga
     1801 gcaaaagctg ccgcagaagc acaggctaaa gcaaaagcaa accgggatgc gctgactcaa
     1861 catctgaagg atattgtgaa tgaggcgctt cgccataatt ccactcatcc ggaggttatt
     1921 gacctggctc atgccaataa tgcagcgatg caggcagaag cagagcggtt gcgccttgca
     1981 aaagcagaag aaaaagcccg taaagaagcg gaagctgcgg aaaaggcttt tcaggaagca
     2041 gaacaacgac gtaaagagat agagaaggag caggctgaaa cagaacgcca attgaaactg
     2101 gctgaggatg aagagaaacg cctggcagca ttgagtgaag aggcccgggc tgtggaggtg
     2161 gcacaaaaaa atcttgctgc tgcacaatct gagctggcga aagtggatga agagattaat
     2221 acgctcaata cccgtttaag ctccagtatt catgcccgtg atgcagaaac gaatacgctg
     2281 tccggaaaac gaaatgagtt ggatcaggca tctgctaaat ataaagaact ggatgaaagg
     2341 gttaaacttt tatctcctag ggcgaatgat ccgcttcaga gtcgtccttt ttttgaggcc
     2401 accagactac gggcgagagc tggtgatgag atggaggaaa aacaaaagca ggtaacagca
     2461 tcagaaacgc gtcttaacca gattagctct gagataaatg gaatccaaga ggctatttct
     2521 caggctaata ataagcgaag tacagcagtt tcacgtattc atgatgctga agataatttg
     2581 aaaacagcac agactaatct cctgaactcg cagattaagg atgctgtgga tgcaacagtt
     2641 agcttttatc aaacgctatc tgaaaaatat ggtgaaaaat attcaaaaat ggcacaggaa
     2701 cttgctgata aatctaaagg taagaaaatc agcaatgtga atgaagctct agctgctttt
     2761 gagaaataca aggatgtatt aaataagaaa ttcagcaaag cagaccgtga tgcaattttc
     2821 aatgcactgg aggcggttaa gtatgaagac tgggctaagc atttagatca gtttgccaag
     2881 tacttgaaga ttacgggaca tgtttctttt ggatatgatg tggtatctga tatcctaaaa
     2941 attaaggata caggtgactg gaagccgcta tttcttacat tagagaagaa agctgtagat
     3001 gctggagtta gttatgttgt tgttttactt tttagtgtgc ttgctggaac tacattaggt
     3061 atctggggga ttgctattgt tacaggcatt ctatgtgcct ttattgataa gaataaactt
     3121 aatactataa atgaggtgtt gggtatttaa ttattcttta ttgatataaa cagtcctagc
     3181 aatccaaatg ggattgctag gaccaaacaa agtagattgt atagcataaa taggtttaat
     3241 tttgccacgg gggcattatt taggtttttt ctttttctga aaaaatcttt ctttatgaac
     3301 ttaaaaaata tgtgttcaat agcatatttt gaatatgggc atagaatagt gcttatcact
     3361 attgcgtata gcatcttatc tgacgctagg aaataatacc cttcgttgtt ttttgttata
     3421 aggtatatat atataagtgc gcagtatagg ccaaacaaaa tattttttat gtagtatctt
     3481 aaactcataa attaaacctc gttatatatt cttttcattt tataaggatc gagttatgag
     3541 gaaaagattt tttgtgggaa tattcgctat aaacctcctt gttggatgtc aggctaacta
     3601 tataggggtt ccgtgggaat ttctgcgata acacccgcta agcgattcct ccctgacgga
     3661 aatcacttgt tttccgtcag gtcctgcagt tgaagaaaat tgaatccata aatctgtgat
     3721 tttttgcgtg acaaaccctg tcggaatgag aaactgtacg gaaacttcaa ttttaaggtt
     3781 tgtcgtacat gttgattggt tatatgcggg tatcaagcaa tgatgaacgt cagtcagttt
     3841 ctttgcagcg tgatgcgttg attgctgctg gagtggatcc ccggcattta catcaggatc
     3901 atgcttcagg tgctcgtgat aatcgcccgg gacttaagtc ctgtctcgct gaacttcgcg
     3961 aaggtgatgt actgatagtc tggaaactgg atcgacttgg tcgttcactc tcacatttgc
     4021 tccgaattat tgaagacctg aaaattcgtg gtattgcttt ccgatcattg actgaatcta
     4081 ttgatacaac aacgccacac ggctcgttcc tctttaacct ttttggcacg ctcgcggagt
     4141 acgaaagaac acttatcaca gaacgcgtta atgctggtct ggctgctgct ctccggcggg
     4201 ggagaaaagg cggaaggcca ccggcgatag atgctgaaaa ggttgatcaa atcctcgcag
     4261 ctctggaagg aggtgccagt aaagcgtcag tatgtcgcgc attcagtgtg ccacgctcaa
     4321 cgctcaccga tactctacga cgaattggct gggccggacc tggggggcgt aatctgacaa
     4381 cggatcagag caatgtcatt tctcgactct aaatcgtacg atacgctttt tggattacct
     4441 gaactttatg aggatgcact tccccggttt tcgctctcct gcgaggatat tgcattcgcc
     4501 agaactcatc gtcgatctca taaccgtctg ggttttgcca ttcagcttgc acttgtccgt
     4561 gatctcggtc gtacactgcg tgcaggagag attcccccgg aggctgttat caccgttgtt
     4621 gcggaacaac tggatattga ccctgctgtt tttacacttt acgcacaacg tgaggaaacc
     4681 cgccgggaac atgcccgcga aatagtttcg gctttggagc ttcgttccgt ccggagtagc
     4741 gattacagaa tgctcattat cgcggctaca cgcgaagcag caaccaccga aaaaggactg
     4801 ccgatcacca gagcagttat caataagctg aaggaccaga agttacttgc ccctgctccg
     4861 gcacaactca tccgtctggc aatggcgggc cgggcggctg cccgtcgcct ttcttatcgg
     4921 gaactcatca gggatctgaa accttcatcc attagcgctc tcgagcagtt gcttaccgaa
     4981 cgagtcggag aaaagagcat tttaggctgg atagctgaag cgccggaagg cgtgaaactc
     5041 aagaatctta aggggttaat tgccaggctg gaggtattac gaacagcggc aatctccgat
     5101 gagcaacgta aacggattca tgtaaaccgt tacggtatta ttgcccggga ttctcgcgtt
     5161 ttacatgcgc gtgaaataca acgaatggca tccgaacgct gtcatgccac gctggtagcc
     5221 tttgttattg aaaagcaggc agcaattaca gatctggcaa ttgatatgtt ctgcaaattg
     5281 attggcagca cgaggcgcag agcagagata agccagaaag agcgtcgtct gaaagccgct
     5341 gaagtacttg aagtcgtagc tcaggatcat attaagttgg gacgggctct cctggcagca
     5401 cgtgacggta atactgatat tgaatctgta ataacagcat cactcggctg ggaaggtttg
     5461 attgccagta tggaggctgc agcttcggtg gcatcttccc atcactgttc tgaactggat
     5521 gaacttatcg gacgacaaaa atcattacgt aagcttggac gactcatttt cagcgctttt
     5581 tctttcagag cttttcgccc ggatgatagc gttttgttgg cgattgggta tttgcaggcg
     5641 ttctatcatg gccgaaaatt acccaaacaa attccgctcg attttatgtc ccataaatgg
     5701 cgacgtcgtg tcatccatgg agaaaatacc gatctgcagg cgtgggaagt cgccgtattc
     5761 gttcatttgc gggaacgcct gaaaacaggt gacatatggg ttgatggtag tcgggcctgg
     5821 cgtagttttg aggactacct tcttcctcac catgttttcg ggcagatgcg ggcagaatcc
     5881 cggctgggac ttgccattcc ggacaacttt gctgaatggc gggctgagcg aaccaccaca
     5941 ctcaacgcaa aactgaaagc gctggcaaaa gctgcttcgg agaatgccat acccgatgca
     6001 gaactttcca gcagagggtt gtctgtgtct ccgattcgcg aagaagagag cgacaaaatt
     6061 aaggctctca gccgtaaact ctacatgctg gtaccacgca tcagaattac cagtttgctg
     6121 gcagaagttc atagctggac cagttttctt gattgtttca cccattatcg caccggggaa
     6181 caggcggaag atgaagctgc acttatggct gcgatccttg ccgatgcgac gaatgcggga
     6241 gcagaacgga tggcgcagag ttcccggggt gtcaccattc accagatgat ggtaatgatg
     6301 gatcgtcatt tacgcactga aacctatgcg tctgccacat cagttctggt cgatgcgcaa
     6361 caggaacatc cttttgctgc tatatggggg gatgggcatg tgtcatcctc ggacgggcag
     6421 ttttttcctg cgggtggacg aggagaagcc agcctcgaat ataacgccaa atacggcaaa
     6481 cgacctggcg cgtcgattta tggtttttta tcgaaccgtt ttgcatcctt tttttccaga
     6541 atgattcagg cctctgaaag cgaggctccc tacgtacttg acggcttact tcataacgaa
     6601 agttctgtgg taattcatga gcatgccacc gatacagccg gtgcgactga aacaacattt
     6661 tcaatgtttc actcttttgg ttacagactc ataccccgta ttcgcaacct cggcagtcgc
     6721 aggttgtacg ttatttcccc ctcagaggaa tataaaccac ttgatgccct gatcgccgga
     6781 aaggccaaaa tggaagtaat agaacagcac tgggatgaag ttctgcggct taaggcctcg
     6841 atcggcgctg gtctggtacc accatcggta atactgaaaa agctggcagc atctccgaga
     6901 caaaacaggc tcaatctggc cctgcgtgaa atgggacgta ttgaacgctc aatttttatc
     6961 tgtgactggt tgcttgacac tcggttacgt cgcagatcac acgcaatcct caacaaaggc
     7021 gaaagccgcc atgcacttgc ccgcgctatt tttcttcacc aactgggcga attccggaac
     7081 agggctgccg agacaatggc tcacagggca tcgggcctca accttgttgt taatgcaata
     7141 attctgtgga acacggtcta tctcagtcgg gctgttgatt acattcgcag ccaggggatc
     7201 attataccgg aagagctctt gtccagtgta tcaccactac catggggtca tatctcgctc
     7261 actggagatt acctgtggaa cgaaattgac catccccttg aacgatacag gccacttcgt
     7321 actaatcgct tcaatccaaa gaacttcact tttccttagc gtgtgttatc gcagaaatgc
     7381 ccacggaacc ccatatacgt gatgttcagg gagggactgt ggctccatca tcttcttcta
     7441 aattgacggg gatctctgtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt
     7501 tctgcgcgta atctgctgcc tgtaaacgaa aaaaccgcct ggggaggcgg tttgatcgaa
     7561 ggttaagtca gttggggaac tgcttaaccg aggtaactgg cttcagcaga gcgcagatac
     7621 caaatactgt ccttccagtg tagccgtagt cgggccacta cttcaagaac tctgttgcac
     7681 catttgcgcc atcatcgctc tgctaaccaa gttaccaacg gctgctgcca gtggcgctaa
     7741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgagccgag
     7861 ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt
     8041 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc atcggcgtgg ccttttgttg
     8101 ggctttgcct ttttgcgcac atgttctttc cggtcttatc cccggattct gtggataact
     8161 gtattatcgt ttttgagtga gtcagtaccg ctcgccgcag ccgaacgacc gagcgtagcg
     8221 agtcagtgag cgaggaagcg gaaaagcgcc ggaacgtgca ttttctcctt acgcatctgt
     8281 gcggtatttc acactggtca tggtgcactt ttcatacaat ccgcactgat gctgcatagt
     8341 taagccagta tacactccgc tatcgctacg tgactggttc agggctgcgc cccgaaaccc
     8401 gctaaaacac cctgacgcgc cctggcgggc ttgtctgctc ccggcatccg ctcacaggca
     8461 agctgtgacc gtctccggga gctgcatatg tcagaggttt tcactgtcat ccccgaagcg
     8521 cgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac agatatctgc
     8581 ctgttcatcc gcgtccagtt cgttgagctt ctccagcagc gttaatgtct ggcttctgat
     8641 aaagcgggcc atgttaaggg cggttttttc ctgtttagtc actgatgcct ccgtgtaagg
     8701 gggatttctg ttcatggggg taatgatacc gatgaaacgc gagaggatgc tcacgatacg
     8761 ggttactgat gatgaacatg cccggttact ggaacgctgt gagggtaaac aactggcggt
     8821 atggatgcgg cgggtctgcc tgggtgagcc ggttgcccgt tccgggaggc tgccgacact
     8881 ggcaccgccg ttactgcgtc agctggcagc catcgggaat aacctgaacc agacagcccg
     8941 taaggtgaac agcggacagt ggtcttccgg cgaccgggtt caggtggtgg ccgcactgat
     9001 ggccatcggg gatgagctgc gccggttgcg tctggccgtc agggaacagg gggcgcggga
     9061 tgatagttaa gtttcatgcc aggggaaaag gtggtggcag tggtccggtt gattacctgc
     9121 tgggaaggga gcgtaaccgg gaaggggcaa cggtgcttca gggtaatccg gaagaagtcc
     9181 gggaactcat cgatgccacg ccatttgcga agaaatacac gtccggtgtt ctgtcgttcg
     9241 cggagaagga gctgccgccg ggaggacgtg aaaaagtgat ggcgagcttt gagcgtgtac
     9301 tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg ggtggagcac caggacaagg
     9361 gacggcttga gctgaatttt gtcattccga acatggagct ggagaccgga aaacgcctac
     9421 agccgtacta cgaccgggca gacaggccga gaattgatgc ctggcagacg ctggtgaatc
     9481 accattacgg gctgcatgac ccgaacgccc cggagaaccg ccggacgctg actctccctg
     9541 ataacctgcc ggaaacgaaa caggcgtttg ctgagggcgt cacacgcggg atagatgctc
     9601 tttaccatgc aggagagata aaaggccgtc aggatgtgat tcaggcgctc acggaagcag
     9661 ggattgaagt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg aacggcggga
     9721 agaatatcag actgaaagga gcattctatg agcaatcttt tacagacggg cgcggagttc
     9781 gagaaaaagc tgaaagagag agccgaatct acagagacaa tgctgaacga cgagttcagc
     9841 aagctcgaaa aatctgtaag caaggctgtg acatcaaacg agacgaaaat caaagacgct
     9901 atagccctgt tcacagcttc gacagaggaa tcactgaaaa agcaccggga aggggtgaaa
     9961 gaggcgatga tgcagcacag gaaggacgtg ttaaagctgg cagggaatac gggcatgatg
    10021 ttactgggga tggtcatttt cctgtttacc gtgagttgcg ggacgctctg gtatcttgga
    10081 gggatgatac aggcgaacct ggaagatatc aggaagcagg aagagacatt gcagaaactg
    10141 aacgcgaaga catggggcgt ggagttcgta caggacggga acaggaaatt tctggtcctt
    10201 ccgtacggga aatcagcaga ggtgattccc catcagggga aggactgggt acaactgatg
    10261 gagtgacaca gagtgacgga gctggaaaga catttgctga acgcattaga gcaactgcaa
    10321 caggactata tgcagcggct gaacgaatgg gagagcgcct tcgtggaatt gcagaaaatg
    10381 ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgaa gttgagtcag
    10441 caggtgcagc acttgagcga gcggacagaa cgcttgagcc agttatacag cgagaacagg
    10501 agataaggga cgaacggctg atacaggaac gcgaacatga gttatccctg gagcgtgagc
    10561 gccagctgga aatacaggaa cggacactgg atggcccttc gctgggatgg taatcagaga
    10621 caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt gggataatgg ttggttcggg
    10681 g
//
