LOCUS       NZ_CP148354            10981 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0399 plasmid pZ1323HEC0399-6,
            complete sequence.
ACCESSION   NZ_CP148354
VERSION     NZ_CP148354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441599
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038096675.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 11:28:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0399"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0399-6"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-17"
     gene            complement(437..643)
                     /locus_tag="WHU37_RS00005"
                     /old_locus_tag="WHU37_27795"
     CDS             complement(437..643)
                     /locus_tag="WHU37_RS00005"
                     /old_locus_tag="WHU37_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_027663146.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGELMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(636..1019)
                     /locus_tag="WHU37_RS00010"
                     /old_locus_tag="WHU37_27800"
     CDS             complement(636..1019)
                     /locus_tag="WHU37_RS00010"
                     /old_locus_tag="WHU37_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_027663147.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKVVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(1624..2685)
                     /locus_tag="WHU37_RS00015"
                     /old_locus_tag="WHU37_27805"
     CDS             complement(1624..2685)
                     /locus_tag="WHU37_RS00015"
                     /old_locus_tag="WHU37_27805"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_069201238.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     KNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            2773..3930
                     /locus_tag="WHU37_RS00020"
                     /old_locus_tag="WHU37_27810"
     CDS             2773..3930
                     /locus_tag="WHU37_RS00020"
                     /old_locus_tag="WHU37_27810"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4158..4316)
                     /locus_tag="WHU37_RS00025"
                     /old_locus_tag="WHU37_27815"
     CDS             complement(4158..4316)
                     /locus_tag="WHU37_RS00025"
                     /old_locus_tag="WHU37_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(4977..5168)
                     /locus_tag="WHU37_RS00030"
                     /old_locus_tag="WHU37_27820"
     CDS             complement(4977..5168)
                     /locus_tag="WHU37_RS00030"
                     /old_locus_tag="WHU37_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5233..5556
                     /locus_tag="WHU37_RS00035"
                     /old_locus_tag="WHU37_27825"
     CDS             5233..5556
                     /locus_tag="WHU37_RS00035"
                     /old_locus_tag="WHU37_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_000955995.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            5546..>5980
                     /locus_tag="WHU37_RS00040"
                     /old_locus_tag="WHU37_27830"
                     /pseudo=""
     CDS             5546..>5980
                     /locus_tag="WHU37_RS00040"
                     /old_locus_tag="WHU37_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6008..8974)
                     /locus_tag="WHU37_RS00045"
                     /old_locus_tag="WHU37_27835"
     CDS             complement(6008..8974)
                     /locus_tag="WHU37_RS00045"
                     /old_locus_tag="WHU37_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_072024409.1"
                     /translation="MSFLDSKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFR
                     AFRPDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVH
                     LRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTL
                     NAKLKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLL
                     AEVHSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVM
                     MDRHLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKY
                     GKRPGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATE
                     TTFSMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLR
                     LKASIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHA
                     ILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDY
                     IRSQGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     gene            complement(8955..9578)
                     /locus_tag="WHU37_RS00050"
                     /old_locus_tag="WHU37_27840"
     CDS             complement(8955..9578)
                     /locus_tag="WHU37_RS00050"
                     /old_locus_tag="WHU37_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_077150956.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRL"
                     /name="recombinase family protein"
     gene            10027..10545
                     /locus_tag="WHU37_RS00055"
                     /old_locus_tag="WHU37_27845"
     CDS             10027..10545
                     /locus_tag="WHU37_RS00055"
                     /old_locus_tag="WHU37_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_096933969.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIRDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            10552..10806
                     /locus_tag="WHU37_RS00060"
                     /old_locus_tag="WHU37_27850"
     CDS             10552..10806
                     /locus_tag="WHU37_RS00060"
                     /old_locus_tag="WHU37_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_161399083.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQQDNA
                     MLNEQVMQLSQQVQHLSERTERLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4815..4899
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          complement(4245..4795)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.404"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5547..5980
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.621"
                     /coverage="100.0"
                     /evalue="2.82e-88"
     Typing          9763..10910
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.817"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaccgggaa cgcgcttcag tcagaccttg ctgaaaacac aggaacgcgt taaaacgcat
       61 acgggcgcga ttctggtgtg tcttttgttg ctgtgttttg cctctattgc cagatcgcgc
      121 tcattcccct gaacttctgt tttcccgctc cggtccccgt cttcttcgct tccgaaagca
      181 tgaaaaaaat ccgtttcagc agtctctggc tggcaattcc tgcgtgattc acatggctgc
      241 atagctatgc atgtggttaa aatttaccgg gtgcgatcgc ggcagttttt cgggtggttt
      301 gttgccattt ttacctgtct gctgccgtga tcgcaccgaa cgcgtttctg cggtgcgtgc
      361 aacggatgtt atggtaaata caatatgcag ccagtcttag agacgaaaag tccgcccaga
      421 agactgactt ttcagatcaa ccttcagcat ctaacttgtc tatcgctttt ttattgcgcc
      481 gataggtagc cattagttca ccatcgcctg attgtaagtc acgacctgct tgctcagcat
      541 aggtatcgtt tacagtctta gcgccttttt tataggcctt tttaacatca tcaaatccgg
      601 cttggatgac ttctttcggc ttttcttgag gtaatttagc catgctgtga atcctctata
      661 aggttgatta tttttccaac agataagacg gcatcttctg tgtcgctgat tttatgattt
      721 atccaaccgt cctctatctt taatttgttc actactccta atgtaaaaac aacctttttt
      781 tcaggaaaga aaattgcaaa ggttctactt ttctctttcc aatcagaggg cggcgtcgaa
      841 cggaacgcta cattcctcga tttaagtatc gctttgattt tactaattaa ttcttttttt
      901 tgtttattgc tgagattttt gacgaaccct ttgctgagat tgtctgtgta atcatcaaaa
      961 gaaatatcaa ctggtttgtt tttttgtttt ttatcagata gatgaacaat gtttgacact
     1021 ggatatcctg ctttttgatg aatgaatact cagaagagta gcggaactgt cttgttagtc
     1081 acagaggtag agtatcaatt ctacgataga tgggttcgaa gatccggctg agaccgttca
     1141 gtacgcggtc gagatgttat gttggggtct gaagtccaat ggaacgaaaa cgtacgttag
     1201 tgaagtaact gtatgatata tcaagacata atagacatag aaaaacgcca tcaaaacggt
     1261 gtctttttaa tcgaaaattg gcacttaacg gacggcattg tctactaatc gatttttaag
     1321 acgataattt tcctcttacc gtacattttc gtacggttgg ccctcaaacc ccatgttcaa
     1381 tgtaatgacc gtcgaaattg cctttacggg catgaagcaa ccaggagcaa tcattggcaa
     1441 aggtaactag agttcaatgc atggtaatca tttttgagct tgtgatgcat agagttggtt
     1501 gctgttccat tgcgtctcaa actccgtcaa gatgctcgcc gtaaaagcga gcatgtctag
     1561 tatgcctgtt ccgctactga tttttgatgg gcgaattcag ggctggcgag ccaccaagaa
     1621 aaattacttt atttcatcaa acaggtgaga tgcagtataa taaacgcttt gatcaaagcg
     1681 tctctctggg cttaatttat tttgcccgcc atttaaaatt atttctttta atactgatgg
     1741 tgggaggatg ggttttgttc ttcttgggtg atccatataa gcttttgtta tttttccgtt
     1801 tagcgagaac tctttatcac tttgatatcc aaagttcatc tcagtataaa ttctattgat
     1861 gtttgtcttg tttttgctcg caatttcata caccattctg gcgagtctgg ctgatgatct
     1921 cattagtctg ctggcagatg aattgttata tgaggatcta tcaataaagg ttcgacacaa
     1981 agcaaaatca ctccagatga atatgtcaaa ggcattttga tctaatattg ggctcttgcc
     2041 ttttgttttc cataatggtt gcattaaaaa aggtttctgg taatcaagaa aattttcttg
     2101 gaaagtgttt aggcaattta atatattttc tttctttgaa agaacttcag tttcattccc
     2161 ccagtgctgt atgctttcgc atgtggattc aaatattttt cttatttcat ggaatttatc
     2221 gctgcaagag tgcattatgc caagggcaca ataagaagtg cttgcaggtc ttattacaat
     2281 ctctgaactc cattcgctct cgttttcttt atatgtcgtg ctatctggaa gtacggttaa
     2341 ttttatttct agtgctttta aaaaactatt ttttcctttt ataactagat ctattccttt
     2401 tatgtcatca taagcatact gttggtatgg ttcatatttt ttctcaaatt gaaattcaag
     2461 atcactattt tttaatgttg tgttaaaaac gaagtcaaaa gatacttctt tggcatgcac
     2521 ttttaaatcc ttatctaaat aaagataaat cggattcatt ttgttgtctc tcatataaca
     2581 tgctagagag gtgggaaaag aactgttaaa ttggttcttt ccccacagat cgctctcttt
     2641 cctgttggtg ttttttaaac cataaagaga ttctattttg ctcacttttt gctcctgacg
     2701 tcaatttatt gacgtctatg gtgtgtttct atatcatgat gtggagtgat atgataatga
     2761 gttttttatg ttatgagaat aattgatctt tttagtgggt gtggggggtt atccctaggc
     2821 tttttgaagg gggggtttga tgttgtaggt gcttatgatt tctgggatcc tgctattgaa
     2881 tgttacagag ataattttag ccatccgata aaaaaactag atttgagcaa tgttgatgat
     2941 gtggtgaggg agcttaaaga tatagacttc gatatgatta ttggagggcc tccttgccaa
     3001 gacttcagtc atgcaggttt aagaattgaa ggtgctagag ctaacttaac tcgctcattt
     3061 tcagaaatta taaaaagaat aaagccaaag tggtttgtga tggaaaatgt agatcgggca
     3121 ttaagaagtg gtgcctattt agaagctcga ggaatcttta aagaatcagg gtatgggtta
     3181 acagaaattg ttttggatgc cagtaagtgt ggtgttccgc aaaaaagaaa aagattattt
     3241 gtaattggaa agcttgacgt gagggatggt tttatcctta acgaagtgat gtgcgggata
     3301 tcgaaagata gtatgacagt tagaaattat cttggtgatt cattggggat tgagtattat
     3361 tacagacatc cgagaaatta taacagaagg gctattttct ctattgatga acctgcgcca
     3421 acagtaagag gggttaatag accgatacct gatggatatt tagggcatgc aggcgatcct
     3481 gtcagtattt ctgagaatgt aaggccatta acaacctttg aaagagcaag attgcaaaca
     3541 tttcctgaag actttaaatt taaaggtgca aaaacaaatt tggagcaaat gataggtaat
     3601 gctgttccgg ttgagttagc aaaatatgtt gcggtgacaa tcatggagta tgaaaaaaaa
     3661 caagtaaaag gtatttatga taaagaaggc tttagggcgt ggttgctgaa tgaaaagaaa
     3721 ctaacaaaaa gaacctctag cgatattata tctagatgtt gcagaggtgt ttcatttttt
     3781 gattcagagg gggtggattt ttataactgt gaaatagatg aaattattat gaaactggaa
     3841 aggcttgaat cgtttgtcag gcttggggtt agcctgaagt ctcagttaag aagggctttt
     3901 aaattatatt acgaatattg tcgaaggtaa taaatacgaa tttatgtctc tcaggggagg
     3961 gggaggggat aaaaagatca aaggatcctc ttgtgatcct ttttttctgc gcgtaatctg
     4021 ctgcctgtaa acaaaaaaac caccgctacc aacggtggtt tgtttgccgg aatctagagc
     4081 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatactgtcc
     4141 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     4201 gtccagttac cagtggctgc tgccagtggc gctttgtcgt gtcttaccgg gttggactca
     4261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     4381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4501 gggtttcgcc acctctggct tgagcgtcga tttctgtgat gctcgtcagg ggggcggagc
     4561 ctatggaaaa acgccagcgg cgcggccttt tgttgtcctt tgcctttttg ctcacatgtt
     4621 ctttccggtc ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagccga
     4681 ctccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     4741 gcgccggatg cggtattttc tccttacgca ccatatgcgg tatttcacac cgcatatggt
     4801 gctcttttct tataatctgc actgatgcca catagttaag ccagtataca ctccgctatc
     4861 gctacgtgac tggttcaggg cttcgccccg aaaccccctg acgcgccctg acgggcttgt
     4921 ctgctcccgg catccgctca cagacaagct gtgaccgtct ccgggagctg tatgtgtcag
     4981 aggttttcac cgtcatcccc gaagcgtgcg aggcagctgc ggtaaagctc atcggcgtgg
     5041 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagttcgtt gagcttttcc
     5101 agcagcgtta atgtctggct tcttataaag cgggccatgt taagggcggt tttttcctgt
     5161 ttagtcactg atgcctccgt gtaagggggg atttctgttc atggggtaat gataccgatg
     5221 aaacgcgaga ggatgctcac gatacgggtt actgatgatg aacatgcacg gttactggaa
     5281 cgctgtgagg gtaaacagct ggcggtatgg atgcggcggg tctgtctggg tgagccggtt
     5341 gcccgttccg ggaagctgcc gacactggca ccgccgttac tgcgccagct ggctgccatc
     5401 gggaataacc tgaatcagac ggcgaggaag gtgaacagcg ggcagtggtc ttccggtgac
     5461 cgggttcagg tggtggccgc actgatggcc atcgagagag agttgcgcag cttacggcag
     5521 gtcgtcaggg aacatggagc gcgggatgat agttaagttt catgccaggg gaaaaggcgg
     5581 tggcagtggt ccggttgatt acctgctggg aagggagcgt aaccgggaag gggcaacggt
     5641 gcttcagggt gatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa
     5701 atacacgtca ggtgttctgt cgttcgcgga gaaggagctg ccgccgggag gacgtgaaaa
     5761 ggtgatggcg agctttgagc gtgtactgat gcccggtctc gaaaagaatc agtacagcat
     5821 cctgtgggta gagcaccagg acaagggacg gcttgagctg aattttgtca ttccgaacat
     5881 ggagctacag accggaaaac gcctccagcc gtactacgac cgggcagaca ggccgagaat
     5941 tgatgcctgg cagacgctgg tgaatcacca ttacggggtt ccgtgggcat ttctgcgata
     6001 acacacgcta aggaaaagtg aagttctttg gattgaagcg attagtacga agtggcctgt
     6061 atcgttcaag gggatggtca atttcgttcc acaggtaatc tccagtgagc gagatatgac
     6121 cccatggtag tggtgataca ctggacaaga gctcttccgg tataatgatc ccctggctgc
     6181 gaatgtaatc aacagcccga ctgagataga ccgtgttcca cagaattatt gcattaacaa
     6241 caaggttgag gcccgatgcc ctgtgagcca ttgtctcggc agccctgttc cggaattcgc
     6301 ccagttggtg aagaaaaata gcgcgggcaa gtgcatggcg gctttcgcct ttgttgagga
     6361 ttgcgtgtga tctgcgacgt aaccgagtgt caagcaacca gtcacagata aaaattgagc
     6421 gttcaatacg tcccatttca cgcagggcca gattgagcct gttttgtctc ggagatgctg
     6481 ccagcttttt cagtattacc gatggtggta ccagaccagc gccgatcgag gccttaagcc
     6541 gcagaacttc atcccagtgc tgttctatta cttccatttt ggcctttccg gcgatcaggg
     6601 catcaagtgg tttatattcc tctgaggggg aaataacgta caacctgcga ctgccgaggt
     6661 tgcgaatacg gggtatgagt ctgtaaccaa aagagtgaaa cattgaaaat gttgtttcag
     6721 tcgcaccggc tgtatcggtg gcatgctcat gaattaccac agaactttcg ttatgaagta
     6781 agccgtcaag tacgtaggga gcctcgcttt cagaggcctg aatcattctg gaaaaaaagg
     6841 atgcaaaacg gttcgataaa aaaccataaa tcgacgcgcc aggtcgtttg ccgtatttgg
     6901 cgttatattc gaggctggct tctcctcgtc cacccgcagg aaaaaactgc ccgtccgagg
     6961 atgacacatg cccatccccc catatagcag caaaaggatg ttcctgttgc gcatcgacca
     7021 gaactgatgt ggcagacgca taggtttcag tgcgtaaatg acgatccatc attaccatca
     7081 tctggtgaat ggtgacaccc cgggaactct gcgccatccg ttctgctccc gcattcgtcg
     7141 catcggcaag gatcgcagcc ataagtgcag cttcatcttc cgcctgttcc ccggtgcgat
     7201 aatgggtgaa acaatcaaga aaactggtcc agctatgaac ttctgccagc aaactggtaa
     7261 ttctgatgcg tggtaccagc atgtagagtt tacggctgag agccttaatt ttgtcgctct
     7321 cttcttcgcg aatcggagac acagacaacc ctctgctgga aagttctgca tcgggtatgg
     7381 cattctccga agcagctttt gccagcgctt tcagttttgc gttgagtgtg gtggttcgct
     7441 cagcccgcca ttcagcaaag ttgtccggaa tggcaagtcc cagccgggat tctgcccgca
     7501 tctgcccgaa aacatggtga ggaagaaggt agtcctcaaa actacgccag gcccgactac
     7561 catcaaccca tatgtcacct gttttcaggc gttcccgcaa atgaacgaat acggcgactt
     7621 cccacgcctg cagatcggta ttttctccat ggatgacacg acgtcgccat ttatgggaca
     7681 taaaatcgag cggaatttgt ttgggtaatt ttcggccatg atagaacgcc tgcaaatacc
     7741 caatcgccaa caaaacgcta tcatccgggc gaaaagctct gaaagaaaaa gcgctgaaaa
     7801 tgagtcgtcc aagcttacgt aatgattttt gtcgtccgat aagttcatcc agttcagaac
     7861 agtgatggga agatgccacc gaagctgcag cctccatact ggcaatcaaa ccttcccagc
     7921 cgagtgatgc tgttattaca gattcaatat cagtattacc gtcacgtgct gccaggagag
     7981 cccgtcccaa cttaatatga tcctgagcta cgacttcaag tacttcagcg gctttcagac
     8041 gacgctcttt ctggcttatc tctgctctgc gcctcgtgct gccaatcaat ttgcagaaca
     8101 tatcaattgc cagatctgta attgctgcct gcttttcaat aacaaaggct accagcgtgg
     8161 catgacagcg ttcggatgcc attcgttgta tttcacgcgc atgtaaaacg cgagaatccc
     8221 gggcaataat accgtaacgg tttacatgaa tccgtttacg ttgctcatcg gagattgccg
     8281 ctgttcgtaa tacctccagc ctggcaatta accccttaag attcttgagt ttcacgcctt
     8341 ccggcgcttc agctatccag cctaaaatgc tcttttctcc gactcgttcg gtaagcaact
     8401 gctcgagagc gctaatggat gaaggtttca gatccctgat gagttcccga taagaaaggc
     8461 gacgggcagc cgcccggccc gccattgcca gacggatgag ttgtgccgga gcaggggcaa
     8521 gtaacttctg gtccttcagc ttattgataa ctgctctggt gatcggcagt cctttttcgg
     8581 tggttgctgc ttcgcgtgta gccgcgataa tgagcattct gtaatcgcta ctccggacgg
     8641 aacgaagctc caaagccgaa actatttcgc gggcatgttc ccggcgggtt tcctcacgtt
     8701 gtgcgtaaag tgtaaaaaca gcagggtcaa tatccagttg ttccgcaaca acggtgataa
     8761 cagcctccgg gggaatctct cctgcacgca gtgtacgacc gagatcacgg acaagtgcaa
     8821 gctgaatggc aaaacccaga cggttatgag atcgacgatg agttctggcg aatgcaatat
     8881 cctcgcagga gagcgaaaac cggggaagtg catcctcata aagttcaggt aatccaaaaa
     8941 gcgtatcgta cgatttagag tcgagaaatg acattgctct gatccgttgt cagattacgc
     9001 cccccaggtc cggcccagcc aattcgtcgt agagtatcgg tgagcgttga gcgtggcaca
     9061 ctgaatgcgc gacatactga cgctttactg gcacctcctt ccagagctgc gaggatttga
     9121 tcaacctttt cagcatctat cgccggtggc cttccgcctt ttctcccccg ccggagagca
     9181 gcagccagac cagcattaac gcgttctgtg ataagtgttc tttcgtactc cgcgagcgtg
     9241 ccaaaaaggt taaagaggaa cgagccgtgt ggcgttgttg tatcaataga ttcagtcaat
     9301 gatcggaaag caataccacg aattttcagg tcttcaataa ttcggagcaa atgtgagagt
     9361 gaacgaccaa gtcgatccag tttccagact atcagtacat caccttcgcg aagttcagcg
     9421 agacaggact taagtcccgg gcgattatca cgagcacctg aagcatgatc ctgatgtaaa
     9481 tgccggggat ccactccagc agcaatcaac gcatcacgct gcaaagaaac tgactgacgt
     9541 tcatcattgc ttgatacccg catataacca atcaacatgt acgacaaacc ttaaaattga
     9601 agtttccgta cagtttctca ttccgacagg gtttgtcacg caaaaaatca cagatttatg
     9661 gattcaattt tcttcaactg caggacctga cggaaaacaa gtgatttccg tcagggagga
     9721 atcgcttagc gggtgttatc gcagaaattc ccacggaacc ccattacggg ctgcatgacc
     9781 ccaacgcccc ggagaaccgc cggacgctga cccttcctga taacctgccg gaaacgaaac
     9841 aggcgcttgc agagagcgtc acacgcggga tagacgctct ttatcatgtc ggggagataa
     9901 aaggccgtca ggatgtgatt caggcgctca cagaggcagg gcttgaagtg gtcagggtga
     9961 cgcgaagcag tatcagcatt gcagacccga acggtgggaa gaatatcagg ctgaaaggag
    10021 cattttatga gcaatctttt acagacgggc gcggagttcg agaaaaagct gaaagagaga
    10081 gccgaatcta cagagacaat gctgaacgac gagttcagca agctcgaaaa atctgtaagc
    10141 aaggctgtga catcaaacga gacgaaaatc agagacgcta tagccctgtt cacagcttcg
    10201 acagaggaat cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg
    10261 aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat ggtcattttc
    10321 ctgtttaccg tgagtggcgg gacgctctgg tatcttggcg ggatgataca ggcgaacctg
    10381 gaagaaatca ggatacagga agaaacattg cagaaactga acgcgaagac atggggcgtg
    10441 gagtttgtgc aggacgggag aaggaaattt ctggtcatcc cgcaggggaa atcggcaacg
    10501 gtgattccct atcaggggaa ggactgggta caactgacgg agtgacacag agtgacggag
    10561 ctggaaagac atttgctgaa cgccttagag caactgcaac aggactatat gcagcggctg
    10621 aacgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacaggac
    10681 aacgcgatgc tgaacgagca ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag
    10741 cggacagaac gcttgagcga gcggacagaa cgcttgagcc agttatacag cgagaacagg
    10801 agataaggga cgaacggctg atacaggagc gcgaacatga gttatccctg gagcgtgagc
    10861 gccagctgga aatacaggaa cggacactgg atggcccttc gctgggatgg taatcagaga
    10921 caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt gggataatgg ttggttcggg
    10981 g
//
