LOCUS       NZ_CP148346             2464 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0422 plasmid pZ1323HEC0422-8,
            complete sequence.
ACCESSION   NZ_CP148346
VERSION     NZ_CP148346.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441600
            Assembly: GCF_037543915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037543915.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:17:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,593
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,136
            CDSs (with protein)               :: 5,136
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 331
            CDSs (without protein)            :: 331
            Pseudo Genes (ambiguous residues) :: 0 of 331
            Pseudo Genes (frameshifted)       :: 121 of 331
            Pseudo Genes (incomplete)         :: 209 of 331
            Pseudo Genes (internal stop)      :: 62 of 331
            Pseudo Genes (multiple problems)  :: 55 of 331
            Pseudo Genes (short protein)      :: 3 of 331
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148346.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0422"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0422-8"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-18"
     gene            1352..1897
                     /locus_tag="WHU23_RS27950"
                     /old_locus_tag="WHU23_27955"
     CDS             1352..1897
                     /locus_tag="WHU23_RS27950"
                     /old_locus_tag="WHU23_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002030412.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_021535322.1"
                     /translation="MSKSKALNINKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMIN
                     QSTGEVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWAVQNKTNND
                     LVLLDQYTLNDFINENKGLSMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVFNGD
                     RVAFTTVLEMNKSSHKDK"
                     /name="replication/maintenance protein RepL"
     gene            complement(1977..2402)
                     /gene="mobA"
                     /locus_tag="WHU23_RS27955"
                     /old_locus_tag="WHU23_27960"
     CDS             complement(1977..2402)
                     /gene="mobA"
                     /locus_tag="WHU23_RS27955"
                     /old_locus_tag="WHU23_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_021535321.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLLKQEIKIIKRHHKNKYPGSNIFS"
                     /name="mobA"
     Typing          complement(686..946)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.221"
                     /coverage="100.0"
                     /evalue="4.01e-96"
ORIGIN
        1 catgcaagcg aatgtattcg cggtatgacg aatacacatc ctgtcccgtt ttccgctata
       61 acttttcgac gaaaataaaa taccgcgtcg taacctggta tttctgatac aaaaaaatga
      121 tgcttcattc aatattattt acacagattt taaaaattat tgtttgttat ttaataaata
      181 acgccgaatt aattgatgtc tttgttgtct gtcgtttgtg gattattggt ggaataaaga
      241 ttagatcttc ctggttatat aatcctttgc ctgttagtga tacggagtgt gtccaagtaa
      301 caggaagcgg aagagcaaga tagtagcatg tacataaaat ataaatacgt gagttattga
      361 tatcgcgttt atatattaaa aatataactt aacattgctc ttaatattta ttttgtcctg
      421 acaatgggag cattaatccc cgtgcagtgc tccgccagct gctgacaacc tcgacatata
      481 accatcgata cgatagaaca tcagggcgcg tttccggcaa gaactaacca tggggatggt
      541 ttgcgtgcca acgaacgccg gaaacgcgct tcaaacctac cgagtcgcca cggttccggc
      601 agcgccttga gcgcaaccgg aaaccgtctg gggagagccc cagaccccca ccgctgcaac
      661 accgacggtc tatgaccacc caccaaccga gtagctgcct gtaccgtgca aatgggcttt
      721 gcagggtgct ggcgcggttc ctggcgacct gaaagcccct ggttaggggt atcggcgcgc
      781 ctggcctcct gaaaccctct gtaagcgttt ttcgcgcctt ccatgcccta gcgggcattt
      841 aaaacccgtt tcgtgcgtct gagagcttct gagagcgttt taggggctat ctggcaactc
      901 atccccgaaa gagcaaccgc ggaatcaccg acaacatgcg agaggtaatt ccgctcgccg
      961 caagtcgatg accgagcgta gcgagcgaat cgacgaggaa gcggaagaga accggaagct
     1021 acattgagca cttacgcaca gaatttggcc tacgcccccg cgtccgtgca cgcgtttaat
     1081 aagaattaag aagggaaatc gcgataaacc gcgccaatcc tagaatctaa tgatcaggat
     1141 attgatctaa cgtatcagaa tagagcatct aacatatcaa ttagttgata tttaaagtat
     1201 tgattgtttg ttttttgctc atcgcgagtg tgatttattt gatctttaag gtatcaaaac
     1261 tatgatacta gtcggatcgg tttattgaca tttgtagatt ttttcttgat agtcgaggta
     1321 tcagtttatt gatacctgag agtgtaaaat aatgagcaag agcaaggctt taaacataaa
     1381 caaacgattt agtgaaaatc ctttcatcaa agatttgata attcctgtaa aaaacaaaca
     1441 agtaagaata tcgcgattag gagaagataa taacataatg attaaccaat caactggaga
     1501 ggttttaggg ggaacctcgg tagtaactta taaaaaagtt gatagtgata attttattaa
     1561 attgtttacc caaaatataa aattggcttt cgagttaaca caagctggtc aaaaagcact
     1621 atttgtttta ttttgggcag tacaaaataa aacaaataat gatctagtct tattagatca
     1681 atacacgcta aatgatttta taaatgaaaa caaaggatta agcatgtccc ttccaacatt
     1741 caaacgtggg cttggggagt tggtaaaatc acaaatatta gcaatgaata agcgaaaggg
     1801 attttattat ataaatccta attttgtttt taatggcgat agagttgcat tcacaacagt
     1861 tcttgaaatg aataaatcga gccataaaga caaatgacaa tgtattaagc ccgtcaaagg
     1921 aaaatttcaa atctattggc gggttttaat attcgtaacg gctagcgccg aagattttag
     1981 ctaaatatat ttgagccagg gtatttattt ttatgatgcc gttttattat ttttatttcc
     2041 tgttttaata gatccatttc attatataca tcagaaattc gcttcgttac tattttcatt
     2101 tcatcgcaaa gtaaaattat tttatgcatc acctcgaggt actccttatc cttcggccgc
     2161 tttcccttat caaataattt cttctgcatc cgaccaagtc ggattaattc atctaaatac
     2221 ctggaaggta cgcgctgttt tatttcatta tttaaacttg ccgtcctgat atattctgaa
     2281 attgtcagtc cttcgttctt cgctcgccgt ctgatttcgc ttgcgactct gttatttact
     2341 ctaagggtga taaccttagt tctctgccgt acttctgaac cactacgttt atttttagcc
     2401 atcgcaatga ctctccattt tgcacgtttg gggtgtcggg gcttgccctg acaggaggta
     2461 ttct
//
