LOCUS       NZ_CP148345             9029 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0422 plasmid pZ1323HEC0422-7,
            complete sequence.
ACCESSION   NZ_CP148345
VERSION     NZ_CP148345.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441600
            Assembly: GCF_037543915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037543915.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:17:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,593
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,136
            CDSs (with protein)               :: 5,136
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 331
            CDSs (without protein)            :: 331
            Pseudo Genes (ambiguous residues) :: 0 of 331
            Pseudo Genes (frameshifted)       :: 121 of 331
            Pseudo Genes (incomplete)         :: 209 of 331
            Pseudo Genes (internal stop)      :: 62 of 331
            Pseudo Genes (multiple problems)  :: 55 of 331
            Pseudo Genes (short protein)      :: 3 of 331
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148345.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0422"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0422-7"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-18"
     gene            complement(306..878)
                     /locus_tag="WHU23_RS27915"
                     /old_locus_tag="WHU23_27920"
     CDS             complement(306..878)
                     /locus_tag="WHU23_RS27915"
                     /old_locus_tag="WHU23_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="WP_001681717.1"
                     /translation="MEYSTLFSFAIVTLSQTISIGPGVALVINNAFSQGLKSSIKTSIY
                     IRAGEAIVMVISLFTLSSTSSIEQHFYIIKILGGSYLIYIGVMGLINKKNKKSKVQKPL
                     LIPLLNPKAYLFFAALIPAFIDDNTNIALNFFILGVLFIFISFLTDIIYIAISLTIRDK
                     LTPSFSRYISICSSIFILGTGIYFILT"
                     /name="LysE family translocator"
     gene            1477..2508
                     /locus_tag="WHU23_RS27920"
                     /old_locus_tag="WHU23_27925"
     CDS             1477..2508
                     /locus_tag="WHU23_RS27920"
                     /old_locus_tag="WHU23_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_062896869.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSEKQADKGRKG
                     GKNSGAVRWTANDDKRRRALDMYILGASTEDIAEAVGVSRWTVRRWMDSSVEWLLSKQI
                     IKF"
                     /name="replication initiation protein"
     gene            complement(3034..3480)
                     /locus_tag="WHU23_RS27925"
                     /old_locus_tag="WHU23_27930"
     CDS             complement(3034..3480)
                     /locus_tag="WHU23_RS27925"
                     /old_locus_tag="WHU23_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_062864536.1"
                     /translation="MTNREDWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARVERKARDHELYKAAGLMIVARLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            3652..4746
                     /locus_tag="WHU23_RS27930"
                     /old_locus_tag="WHU23_27935"
     CDS             3652..4746
                     /locus_tag="WHU23_RS27930"
                     /old_locus_tag="WHU23_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_000179381.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKARF
                     GETQQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGPVRVA
                     VMNADQVCAILAWRNAEREKLEAEKERDSIIDLKTTLKEALAERDNMPPTLDELGAELK
                     AFVESSFDELNEVMQLNSLIDGALDTMMKQFQVEEERKLQQERLIQAARDEAEKQRELE
                     RERVRRRAYSSPAPSRSRDYGMSR"
                     /name="MobA/MobL family protein"
     gene            4768..5268
                     /locus_tag="WHU23_RS27935"
                     /old_locus_tag="WHU23_27940"
     CDS             4768..5268
                     /locus_tag="WHU23_RS27935"
                     /old_locus_tag="WHU23_27940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338929999.1"
                     /translation="MQLDEHSRKIAALVAYNEKQIKEINRIITLLNERVNDLNTFTDTL
                     EREGKVVSGISYSLRDSVSHVDFYLNKGMEEVSCKLQKSVSDTLTSLLREKLSFTLNDV
                     RQEWTDFEEQTSKEIKESLKQTTRDAEVATGIIRKTMEKYTVTRIVEKVLVLLVGVPWE
                     FLR"
                     /name="hypothetical protein"
     gene            5426..6049
                     /locus_tag="WHU23_RS27940"
                     /old_locus_tag="WHU23_27945"
     CDS             5426..6049
                     /locus_tag="WHU23_RS27940"
                     /old_locus_tag="WHU23_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_077150956.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRL"
                     /name="recombinase family protein"
     gene            6030..8996
                     /locus_tag="WHU23_RS27945"
                     /old_locus_tag="WHU23_27950"
     CDS             6030..8996
                     /locus_tag="WHU23_RS27945"
                     /old_locus_tag="WHU23_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_072024409.1"
                     /translation="MSFLDSKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFR
                     AFRPDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVH
                     LRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTL
                     NAKLKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLL
                     AEVHSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVM
                     MDRHLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKY
                     GKRPGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATE
                     TTFSMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLR
                     LKASIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHA
                     ILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDY
                     IRSQGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     Typing          1478..2253
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.272"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          3653..4743
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.055"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtattatt tttattgtta atgattgctc ctagttttta tgctgaatat ctcaaaatga
       61 atatcggttc tctaatggaa gaacagaaaa aacatgaatc tatgttaaaa gagtggaagg
      121 aaaaagcacc attggcagag ttctctaagt gtggagagta tagggacgta tgcgtgttag
      181 ttgatgataa aagcggatat tattccaatg aacaaacccc aaataaaaaa tatgccatac
      241 tgaaatagtg ttaagtaact catgccgtct tatgactaag gctgcatgag tataaaaacg
      301 gcgtttcacg tcaagataaa ataaatacct gtacctaata taaaaatgga gctacatata
      361 cttatgtacc ttgagaatga cggagttaat ttatccctta tcgtcaagct aatagctatg
      421 taaataatat ctgtcaggaa tgatataaat ataaacaaaa cacccaatat aaagaaattt
      481 aatgcgatat ttgtattatc atctataaat gctggtatta atgccgcaaa gaataaatac
      541 gcctttgggt ttaataatgg tataagtaaa ggtttttgta ctttgctttt tttgtttttt
      601 ttattaatta aacccattac ccctatgtat attaaatagc ttccacctaa gatctttata
      661 atgtaaaagt gttgttctat gctggatgtt gaagataacg tgaaaagaga tattaccatg
      721 acgattgctt cgccagctct tatgtatata cttgttttta ttgatgattt aagtccttgt
      781 gaaaaggcgt tgtttataac caatgcaaca ccaggtccta ttgatatagt ttgagataaa
      841 gttacgatag caaatgaaaa taatgttgaa tattccatat ttacttacca tgatatattt
      901 cgtgggagct tttcatatac atattccata ggaattgcgg ctttcggtaa taaatttata
      961 aatgtggata tgtgctttag tgaattgctg gcggcgattg agtaaaaatt tccgctattc
     1021 caatatattg tcgatatttc gttgtccaga ctgtatatgt ttggaaattt gtttgcatac
     1081 gggttcgttc cgtttagctg gctttttgga tgagaaagtt tgctgttttg acccgttcgc
     1141 gcttctgaag gggggaaacc gatcatgaat cgcttttgtg catagttatg caggcccata
     1201 aaaacgattc tgacgcgttt ttatggttta ccctagtgtt ttcttgtctt tttgagtttt
     1261 ttgcgtcaga aagcgattga gagcgtttta gagggtacgt acaacaggag ttatggtaaa
     1321 tggatcgggt ttgcgggaag actcgacagg ttttgtcgtt gggggtagtg taagcgactg
     1381 aaaaacaaac gcccctgaaa ttcgtgcctc tccgccaaga ttgtcacgag aatacagggg
     1441 cgaggtcggt cgataacctg gaaggaatcg taacacatga gcgccgcgct tcaatacttc
     1501 gaagaaaatt tacctcaccg cccctatcac acagatgatc tcgctttcgg ccttcgcatc
     1561 tccggcaaag ggcgtgcgct tcttgcgcgg tacatccagc agaaccagcc tcatgcgcag
     1621 ttctggctgg tttttgacgt tgaccgcgag ggggcagcga ttgactggag cgaccggaac
     1681 gctcccgcgc cgaacatcac cgtaaaaaat cctgtgaacg gtcatgctca cctgctctac
     1741 gcgctcaata tcgccgtgag aaccgcgccg gacagctcgg ttaaggcgct gaaatacgcc
     1801 gctgcgattg aacgttcgct gtgtgagaaa ctttgtgcag atgtgaacta cagcggactg
     1861 atttgcaaaa atccgttcca cctggaatgg caggtgatgg agtggcgcga ggaagcctat
     1921 accctcgatg aactggctga ttatctcgat ttgagcgcct cagcgcgccg tagcatcgat
     1981 aaacattacg ggatggggcg aaactgccat ctgttcgaaa tgacgcgcaa atgggcttac
     2041 agggcgattc gtcagggctg gccagcattc tcacagtggc ttgatgctgt gattcagcgt
     2101 gtcgaaatgt acaacgcatc gcttcccgtt ccgctttcac cgcctgaatg tcgggctatt
     2161 ggcaagagca ttgcgaaata cacgcacagg aacttcacgc cagaaacttt cgcacagtat
     2221 gtggctgata cgcacacgcc agaaattcag gccgcacgag gtcgcaaggg cggtaaggcc
     2281 aattcgtcgg aaaaacaggc cgacaagggc cgcaagggcg gcaaaaacag tggcgctgtt
     2341 cggtggactg ctaacgacga caaacgtcgt agggcgcttg atatgtacat cttgggagcc
     2401 tcaacagaag atattgcaga ggcggttggt gttagccgtt ggactgttcg acgctggatg
     2461 gatagctctg tagaatggct actgagtaaa caaataatta aattttaatt aacaaaaaga
     2521 acacatcacc ccccctattt tgttattttt ttgttaattt tgggtgcacg aagcaagcct
     2581 atatcagata acagcgcccg aacgggggtt tggtgttttt ttgcgcggtg tctccgaccc
     2641 gctcccacac ccccaaacca aataccgaaa ccctgcaaac caagcgagtg gcggctccgc
     2701 tcgccgcagc cctagcgacc gagccgccag gctcgcgagt gggcgaggaa gcggaagatc
     2761 tacaggagtt acatcaggca cttacgcacc agatcccccc gtgcgtatgc gcgtcgcgaa
     2821 attgggcaat gcacaaatag cctatatcag ataacagcgc cttttcggcg tttttttgag
     2881 cagcgggctt tccggcggtt ttcttctttc agcccagcaa tggtgcggct tacggtatga
     2941 ttgaggtggt cgtgctcgcc gcagtctcat gaccgagcgt agcgagcgaa tgagcgagga
     3001 agcaccaggg aaccggaacg tgaacggagg ctcttacgca ctgtctttgg tgagtagctc
     3061 tttcccgcgt ttttcccatt cctgccattt tggatggttg cgcggtagct ctgcgattcc
     3121 tgcgagtgcg cctaccagtt cggcagcact aaactttggt tttccggttt tgctgtccac
     3181 cagtcgtgcc acaatcatga ggcctgcggc cttgtagagt tcatgatctc gtgcctttcg
     3241 ttccaccctt gcggctgcct tccgttctgc cgctatgatt gccgttactc tggcttttgc
     3301 tgcggctgct ttctctgcgg ttttttctgc ccgtatcagt gcggacaacg ttttttcatc
     3361 cgtcgttgtt cggttttttt tctctgccag taacacaagt aatctctgct gctctgacgg
     3421 tgatttcagt ccgttgatgt aggcaatttt tgcacttaac cagtcttcac gatttgtcat
     3481 ccttacctct gcacttttgc cgttaatgtg agttgttatt gtgcataata tcaccttgtc
     3541 tgcctgcgtg tgagaccgta aaatcatccc tgattaattc agggatgcgc acttagacgt
     3601 ttctctcctt tgtcgagaaa cagtgcgctc ctgcggaggg cttaacggca gatggcaagc
     3661 taccattgca cagttaaaac tggagcaaaa ggcgtagcct taaaacatgc tgattacatt
     3721 tccagatccg gcgaatataa aaactataaa tcgcgggagg atctggaatt ttcttcatcc
     3781 ggtaacatgc catcatgggc gaagaaaaat cccgcagaac tgtggaaagc tgctgatgaa
     3841 tttgaacgaa aaaacgggac ggcataccgt gaaattgaga tcgcattacc gcgagaactg
     3901 acacgtgaac aacggataga gcttgttgaa gatttcgtgc aaaaggaact tggtgatcgc
     3961 catgcctatc agtacgcgat ccacaatcca ccaggtgcaa ttgacgggaa ggaacagcca
     4021 cacgcgcata tcatgttttg cgaacggatt aatgatggta ttgaacgcga tccgcagcag
     4081 ttttttaaac gtgccaacag caaatcacca gagcggggtg gcgcaaaaaa agcacgcttt
     4141 ggcgagacac aacaagagcg aaaatcagcc ctggttgcgc tacgctctca ctgggctgat
     4201 gtgcagaatg aacaccttgc gcgacacggc caccagtcgc gcgttgatca tcgttcgctg
     4261 aaagaacagg gtatcaagcg tacgccggag gtgcatcttg gtcccgttcg tgttgctgtt
     4321 atgaacgctg atcaggtttg tgcaattctg gcatggagaa atgcggaacg ggagaagctg
     4381 gaggccgaga aggaacggga cagcatcatt gaccttaaaa cgaccttgaa ggaagcgtta
     4441 gctgaacggg ataacatgcc accaaccctg gatgagcttg gggcagaatt gaaagcgttt
     4501 gttgaaagct cgtttgatga actcaatgag gtaatgcagc ttaattcatt gattgacggt
     4561 gcgctggaca caatgatgaa gcaattccag gtggaagagg agcggaagtt gcaacaggaa
     4621 cgactaattc aggctgcgcg agatgaagcc gaaaagcaaa gagagctgga gagggaaaga
     4681 gtgcgccggc gtgcgtattc ttcaccagca ccatcacgtt cacgcgatta tggtatgagc
     4741 cgttaagcac tggaggtttt ttattttatg caattggatg aacattcaag aaaaattgct
     4801 gcccttgttg cgtataatga aaagcaaata aaagaaataa acaggataat tactctgctt
     4861 aatgaaagag tgaatgactt aaataccttt acagatactc ttgaacgtga aggtaaagtt
     4921 gtatctggaa tttcttattc cttgagggat agtgtgtctc atgttgattt ttatcttaat
     4981 aaaggaatgg aagaagtatc ctgtaaatta cagaaatctg tctcagacac actaacatca
     5041 ttactccgtg aaaaattatc tttcacactt aatgatgtaa ggcaagagtg gactgatttt
     5101 gaagagcaaa caagtaaaga gataaaggaa tcattaaagc agacgaccag agatgctgaa
     5161 gttgcgacag gtataattag aaaaacaatg gagaagtata cagtaacaag gatagtcgaa
     5221 aaggtacttg tgttgcttgt aggggttccg tgggaatttc tgcgataaca cccgctaagc
     5281 gattcctccc tgacggaaat cacttgtttt ccgtcaggtc ctgcagttga agaaaattga
     5341 atccataaat ctgtgatttt ttgcgtgaca aaccctgtcg gaatgagaaa ctgtacggaa
     5401 acttcaattt taaggtttgt cgtacatgtt gattggttat atgcgggtat caagcaatga
     5461 tgaacgtcag tcagtttctt tgcagcgtga tgcgttgatt gctgctggag tggatccccg
     5521 gcatttacat caggatcatg cttcaggtgc tcgtgataat cgcccgggac ttaagtcctg
     5581 tctcgctgaa cttcgcgaag gtgatgtact gatagtctgg aaactggatc gacttggtcg
     5641 ttcactctca catttgctcc gaattattga agacctgaaa attcgtggta ttgctttccg
     5701 atcattgact gaatctattg atacaacaac gccacacggc tcgttcctct ttaacctttt
     5761 tggcacgctc gcggagtacg aaagaacact tatcacagaa cgcgttaatg ctggtctggc
     5821 tgctgctctc cggcggggga gaaaaggcgg aaggccaccg gcgatagatg ctgaaaaggt
     5881 tgatcaaatc ctcgcagctc tggaaggagg tgccagtaaa gcgtcagtat gtcgcgcatt
     5941 cagtgtgcca cgctcaacgc tcaccgatac tctacgacga attggctggg ccggacctgg
     6001 ggggcgtaat ctgacaacgg atcagagcaa tgtcatttct cgactctaaa tcgtacgata
     6061 cgctttttgg attacctgaa ctttatgagg atgcacttcc ccggttttcg ctctcctgcg
     6121 aggatattgc attcgccaga actcatcgtc gatctcataa ccgtctgggt tttgccattc
     6181 agcttgcact tgtccgtgat ctcggtcgta cactgcgtgc aggagagatt cccccggagg
     6241 ctgttatcac cgttgttgcg gaacaactgg atattgaccc tgctgttttt acactttacg
     6301 cacaacgtga ggaaacccgc cgggaacatg cccgcgaaat agtttcggct ttggagcttc
     6361 gttccgtccg gagtagcgat tacagaatgc tcattatcgc ggctacacgc gaagcagcaa
     6421 ccaccgaaaa aggactgccg atcaccagag cagttatcaa taagctgaag gaccagaagt
     6481 tacttgcccc tgctccggca caactcatcc gtctggcaat ggcgggccgg gcggctgccc
     6541 gtcgcctttc ttatcgggaa ctcatcaggg atctgaaacc ttcatccatt agcgctctcg
     6601 agcagttgct taccgaacga gtcggagaaa agagcatttt aggctggata gctgaagcgc
     6661 cggaaggcgt gaaactcaag aatcttaagg ggttaattgc caggctggag gtattacgaa
     6721 cagcggcaat ctccgatgag caacgtaaac ggattcatgt aaaccgttac ggtattattg
     6781 cccgggattc tcgcgtttta catgcgcgtg aaatacaacg aatggcatcc gaacgctgtc
     6841 atgccacgct ggtagccttt gttattgaaa agcaggcagc aattacagat ctggcaattg
     6901 atatgttctg caaattgatt ggcagcacga ggcgcagagc agagataagc cagaaagagc
     6961 gtcgtctgaa agccgctgaa gtacttgaag tcgtagctca ggatcatatt aagttgggac
     7021 gggctctcct ggcagcacgt gacggtaata ctgatattga atctgtaata acagcatcac
     7081 tcggctggga aggtttgatt gccagtatgg aggctgcagc ttcggtggca tcttcccatc
     7141 actgttctga actggatgaa cttatcggac gacaaaaatc attacgtaag cttggacgac
     7201 tcattttcag cgctttttct ttcagagctt ttcgcccgga tgatagcgtt ttgttggcga
     7261 ttgggtattt gcaggcgttc tatcatggcc gaaaattacc caaacaaatt ccgctcgatt
     7321 ttatgtccca taaatggcga cgtcgtgtca tccatggaga aaataccgat ctgcaggcgt
     7381 gggaagtcgc cgtattcgtt catttgcggg aacgcctgaa aacaggtgac atatgggttg
     7441 atggtagtcg ggcctggcgt agttttgagg actaccttct tcctcaccat gttttcgggc
     7501 agatgcgggc agaatcccgg ctgggacttg ccattccgga caactttgct gaatggcggg
     7561 ctgagcgaac caccacactc aacgcaaaac tgaaagcgct ggcaaaagct gcttcggaga
     7621 atgccatacc cgatgcagaa ctttccagca gagggttgtc tgtgtctccg attcgcgaag
     7681 aagagagcga caaaattaag gctctcagcc gtaaactcta catgctggta ccacgcatca
     7741 gaattaccag tttgctggca gaagttcata gctggaccag ttttcttgat tgtttcaccc
     7801 attatcgcac cggggaacag gcggaagatg aagctgcact tatggctgcg atccttgccg
     7861 atgcgacgaa tgcgggagca gaacggatgg cgcagagttc ccggggtgtc accattcacc
     7921 agatgatggt aatgatggat cgtcatttac gcactgaaac ctatgcgtct gccacatcag
     7981 ttctggtcga tgcgcaacag gaacatcctt ttgctgctat atggggggat gggcatgtgt
     8041 catcctcgga cgggcagttt tttcctgcgg gtggacgagg agaagccagc ctcgaatata
     8101 acgccaaata cggcaaacga cctggcgcgt cgatttatgg ttttttatcg aaccgttttg
     8161 catccttttt ttccagaatg attcaggcct ctgaaagcga ggctccctac gtacttgacg
     8221 gcttacttca taacgaaagt tctgtggtaa ttcatgagca tgccaccgat acagccggtg
     8281 cgactgaaac aacattttca atgtttcact cttttggtta cagactcata ccccgtattc
     8341 gcaacctcgg cagtcgcagg ttgtacgtta tttccccctc agaggaatat aaaccacttg
     8401 atgccctgat cgccggaaag gccaaaatgg aagtaataga acagcactgg gatgaagttc
     8461 tgcggcttaa ggcctcgatc ggcgctggtc tggtaccacc atcggtaata ctgaaaaagc
     8521 tggcagcatc tccgagacaa aacaggctca atctggccct gcgtgaaatg ggacgtattg
     8581 aacgctcaat ttttatctgt gactggttgc ttgacactcg gttacgtcgc agatcacacg
     8641 caatcctcaa caaaggcgaa agccgccatg cacttgcccg cgctattttt cttcaccaac
     8701 tgggcgaatt ccggaacagg gctgccgaga caatggctca cagggcatcg ggcctcaacc
     8761 ttgttgttaa tgcaataatt ctgtggaaca cggtctatct cagtcgggct gttgattaca
     8821 ttcgcagcca ggggatcatt ataccggaag agctcttgtc cagtgtatca ccactaccat
     8881 ggggtcatat ctcgctcact ggagattacc tgtggaacga aattgaccat ccccttgaac
     8941 gatacaggcc acttcgtact aatcgcttca atccaaagaa cttcactttt ccttagcgtg
     9001 tgttatcgca gaaatgccca cggaacccc
//
