LOCUS       NZ_CP148344            10681 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0422 plasmid pZ1323HEC0422-6,
            complete sequence.
ACCESSION   NZ_CP148344
VERSION     NZ_CP148344.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441600
            Assembly: GCF_037543915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037543915.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:17:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,593
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,136
            CDSs (with protein)               :: 5,136
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 331
            CDSs (without protein)            :: 331
            Pseudo Genes (ambiguous residues) :: 0 of 331
            Pseudo Genes (frameshifted)       :: 121 of 331
            Pseudo Genes (incomplete)         :: 209 of 331
            Pseudo Genes (internal stop)      :: 62 of 331
            Pseudo Genes (multiple problems)  :: 55 of 331
            Pseudo Genes (short protein)      :: 3 of 331
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148344.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0422"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0422-6"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-18"
     gene            complement(388..807)
                     /locus_tag="WHU23_RS27855"
                     /old_locus_tag="WHU23_27860"
     CDS             complement(388..807)
                     /locus_tag="WHU23_RS27855"
                     /old_locus_tag="WHU23_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(888..1325)
                     /locus_tag="WHU23_RS27860"
                     /old_locus_tag="WHU23_27865"
     CDS             complement(888..1325)
                     /locus_tag="WHU23_RS27860"
                     /old_locus_tag="WHU23_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            1585..3150
                     /locus_tag="WHU23_RS27865"
                     /old_locus_tag="WHU23_27870"
     CDS             1585..3150
                     /locus_tag="WHU23_RS27865"
                     /old_locus_tag="WHU23_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(3147..3488)
                     /locus_tag="WHU23_RS27870"
                     /old_locus_tag="WHU23_27875"
     CDS             complement(3147..3488)
                     /locus_tag="WHU23_RS27870"
                     /old_locus_tag="WHU23_27875"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            3536..3673
                     /locus_tag="WHU23_RS27875"
                     /old_locus_tag="WHU23_27880"
     CDS             3536..3673
                     /locus_tag="WHU23_RS27875"
                     /old_locus_tag="WHU23_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(4698..4889)
                     /locus_tag="WHU23_RS27880"
                     /old_locus_tag="WHU23_27885"
     CDS             complement(4698..4889)
                     /locus_tag="WHU23_RS27880"
                     /old_locus_tag="WHU23_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4954..5277
                     /locus_tag="WHU23_RS27885"
                     /old_locus_tag="WHU23_27890"
     CDS             4954..5277
                     /locus_tag="WHU23_RS27885"
                     /old_locus_tag="WHU23_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5486..>5917
                     /locus_tag="WHU23_RS27890"
                     /old_locus_tag="WHU23_27895"
                     /pseudo=""
     CDS             5486..>5917
                     /locus_tag="WHU23_RS27890"
                     /old_locus_tag="WHU23_27895"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5955..6473
                     /locus_tag="WHU23_RS27895"
                     /old_locus_tag="WHU23_27900"
     CDS             5955..6473
                     /locus_tag="WHU23_RS27895"
                     /old_locus_tag="WHU23_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001318237.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            6480..6695
                     /locus_tag="WHU23_RS27900"
                     /old_locus_tag="WHU23_27905"
     CDS             6480..6695
                     /locus_tag="WHU23_RS27900"
                     /old_locus_tag="WHU23_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_338929998.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQL"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6728..9694)
                     /locus_tag="WHU23_RS27905"
                     /old_locus_tag="WHU23_27910"
     CDS             complement(6728..9694)
                     /locus_tag="WHU23_RS27905"
                     /old_locus_tag="WHU23_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_072024409.1"
                     /translation="MSFLDSKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFR
                     AFRPDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVH
                     LRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTL
                     NAKLKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLL
                     AEVHSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVM
                     MDRHLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKY
                     GKRPGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATE
                     TTFSMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLR
                     LKASIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHA
                     ILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDY
                     IRSQGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     gene            complement(9675..10298)
                     /locus_tag="WHU23_RS27910"
                     /old_locus_tag="WHU23_27915"
     CDS             complement(9675..10298)
                     /locus_tag="WHU23_RS27910"
                     /old_locus_tag="WHU23_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_077150956.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRL"
                     /name="recombinase family protein"
     Typing          4526..4613
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(3957..4515)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5268..6685
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.389"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 caccgggaac gcgcttcagt cataaacctt gctgaaaaca caggaacgcg ttaaaacgca
       61 tacgggcgcg attctggtgt gtctttcata gtcgtgtttt tgcctctatt gccagatcgc
      121 gctcattccc ctgaacttct gttttcctgc tcaaatcccc gtcttcttcg cttccgaaag
      181 tatgaaaaaa atccgtttca gcggtctctg actggcaatt cctgcgtgat tcacctggct
      241 gcataggtat gcatgtggtt aaaatttacc gggtgcgatc gcggcagttt tccgggtggt
      301 ttgttgccat ttttacctgt ctgctgccgt gatcgcaccg aacgcgtttc ggcggtgcgt
      361 acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac
      421 cgcagaaatc gtgtcagcca gcagcctgga ttttttcggg gtaaggtctt tgctgcggta
      481 atcagtaccc gcaaattgag ttgtgccccg gagtgagttt aaaaattcct ggctgtgaaa
      541 ctgttgaatg cgttgttgca catcactccg tgattttcct gtcgctttat ctttagctcc
      601 catatcccag aactgccgaa aaaaaggcac catcattcgt gttgtgtcgg ctttttgggt
      661 ggaaaagttg ggatcaaaac cgtcatcaga agcatatacg taatgccggg tatgttgttc
      721 cacgctgccc tttggggtac aggctgtgag agctactgtt gctgttatca tggcgataag
      781 acaaagtttg tttccggtac gtctcatgag tgtctcccta ccttaaagta tttattctcg
      841 gtgagttatt atccgaagat tatcttatca tcagtttctg agcgcgttca gtgaccgaac
      901 agcctggacc agaatccccg tttcttttcc tgctccagag cctgcctgag ctgggctatc
      961 tcattctgta gctgtttccg ttctgcttcc tggcgccggc gctccgtatc ctgggcttgt
     1021 ttatcctcaa gcatcagcgt caggcattgt ttcagctcat tcagttcccg gagaatgcgt
     1081 tctgtctgct gatcatgagg tttttctgag tgtccttgag tgtgcctttc tggtgtctca
     1141 ttccgcttta attcgccgta tgcccggatc agttcactgg tttcaaactc ccgtcgtccg
     1201 tcctggctgg tacggtaact gacaagacct gagcgcatat cacggtaaaa ctgtgaacgc
     1261 gatcttccag tcattttcag tgcgtcccgg agtgtgtgcc atgccataaa gttacagtgt
     1321 cccataggtg tctcatctca tggtttcagt aaaacataat gaggtctgag aacggtaatg
     1381 tttgtgctgg tttttgtggc atcgggtgga aatagcacgt ggtgtgggtg gctgtttttt
     1441 tgatcgtttt cacaaaaatg aaaatccaca gggttgacag ggaaaatgca gcggcgtaat
     1501 tttaaactgt atataaaacc agtgattata tatacagtat attgctttta actatttgtt
     1561 tttaatgtca aagaggattt tataatggaa acagctgtag cgtactataa agatggtgtt
     1621 ccttatgatg ataaggggca ggtaatcatt actcttttga atggtaatcc agacgggagt
     1681 ggctctggcg gtggtggtgg aactggaggt agcaaaagtg aaagttctgc agccattcat
     1741 gccacagcta aatggtctac tgctcaattg aagaaaacgc aggcagaaca ggctgcccga
     1801 gcaaaagctg ccgcagaagc acaggctaaa gcaaaagcaa accgggatgc gctgactcaa
     1861 catctgaagg atattgtgaa tgaggcgctt cgccataatt ccactcatcc ggaggttatt
     1921 gacctggctc atgccaataa tgcagcgatg caggcagaag cagagcggtt gcgccttgca
     1981 aaagcagaag aaaaagcccg taaagaagcg gaagctgcgg aaaaggcttt tcaggaagca
     2041 gaacaacgac gtaaagagat agagaaggag caggctgaaa cagaacgcca attgaaactg
     2101 gctgaggatg aagagaaacg cctggcagca ttgagtgaag aggcccgggc tgtggaggtg
     2161 gcacaaaaaa atcttgctgc tgcacaatct gagctggcga aagtggatga agagattaat
     2221 acgctcaata cccgtttaag ctccagtatt catgcccgtg atgcagaaac gaatacgctg
     2281 tccggaaaac gaaatgagtt ggatcaggca tctgctaaat ataaagaact ggatgaaagg
     2341 gttaaacttt tatctcctag ggcgaatgat ccgcttcaga gtcgtccttt ttttgaggcc
     2401 accagactac gggcgagagc tggtgatgag atggaggaaa aacaaaagca ggtaacagca
     2461 tcagaaacgc gtcttaacca gattagctct gagataaatg gaatccaaga ggctatttct
     2521 caggctaata ataagcgaag tacagcagtt tcacgtattc atgatgctga agataatttg
     2581 aaaacagcac agactaatct cctgaactcg cagattaagg atgctgtgga tgcaacagtt
     2641 agcttttatc aaacgctatc tgaaaaatat ggtgaaaaat attcaaaaat ggcacaggaa
     2701 cttgctgata aatctaaagg taagaaaatc agcaatgtga atgaagctct agctgctttt
     2761 gagaaataca aggatgtatt aaataagaaa ttcagcaaag cagaccgtga tgcaattttc
     2821 aatgcactgg aggcggttaa gtatgaagac tgggctaagc atttagatca gtttgccaag
     2881 tacttgaaga ttacgggaca tgtttctttt ggatatgatg tggtatctga tatcctaaaa
     2941 attaaggata caggtgactg gaagccgcta tttcttacat tagagaagaa agctgtagat
     3001 gctggagtta gttatgttgt tgttttactt tttagtgtgc ttgctggaac tacattaggt
     3061 atctggggga ttgctattgt tacaggcatt ctatgtgcct ttattgataa gaataaactt
     3121 aatactataa atgaggtgtt gggtatttaa ttattcttta ttgatataaa cagtcctagc
     3181 aatccaaatg ggattgctag gaccaaacaa agtagattgt atagcataaa taggtttaat
     3241 tttgccacgg gggcattatt taggtttttt ctttttctga aaaaatcttt ctttatgaac
     3301 ttaaaaaata tgtgttcaat agcatatttt gaatatgggc atagaatagt gcttatcact
     3361 attgcgtata gcatcttatc tgacgctagg aaataatacc cttcgttgtt ttttgttata
     3421 aggtatatat atataagtgc gcagtatagg ccaaacaaaa tattttttat gtagtatctt
     3481 aaactcataa attaaacctc gttatatatt cttttcattt tataaggatc gagttatgag
     3541 gaaaagattt tttgtgggaa tattcgctat aaacctcctt gttggatgtc aggctaacta
     3601 tatacgtgat gttcagggag ggactgtggc tccatcatct tcttctaaat tgacggggat
     3661 ctctgttcag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3721 tgctgcctgt aaacgaaaaa accgcctggg gaggcggttt gatcgaaggt taagtcagtt
     3781 ggggaactgc ttaaccgagg taactggctt cagcagagcg cagataccaa atactgtcct
     3841 tccagtgtag ccgtagtcgg gccactactt caagaactct gttgcaccat ttgcgccatc
     3901 atcgctctgc taaccaagtt accaacggct gctgccagtg gcgctaagtc gtgtcttacc
     3961 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4021 tcgtgcacac agcccagctt ggagcgaacg acctacaccg agccgagata ccaacagcgt
     4081 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4141 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4201 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg atgctcgtca
     4261 ggggggcgga gcctatggaa aaacgccatc ggcgtggcct tttgttgggc tttgcctttt
     4321 tgcgcacatg ttctttccgg tcttatcccc ggattctgtg gataactgta ttatcgtttt
     4381 tgagtgagtc agtaccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga
     4441 ggaagcggaa aagcgccgga acgtgcattt tctccttacg catctgtgcg gtatttcaca
     4501 ctggtcatgg tgcacttttc atacaatccg cactgatgct gcatagttaa gccagtatac
     4561 actccgctat cgctacgtga ctggttcagg gctgcgcccc gaaacccgct aaaacaccct
     4621 gacgcgccct ggcgggcttg tctgctcccg gcatccgctc acaggcaagc tgtgaccgtc
     4681 tccgggagct gcatatgtca gaggttttca ctgtcatccc cgaagcgcgc gaggcagctg
     4741 cggtaaagct catcggcgtg gtcgtgaagc gattcacaga tatctgcctg ttcatccgcg
     4801 tccagttcgt tgagcttctc cagcagcgtt aatgtctggc ttctgataaa gcgggccatg
     4861 ttaagggcgg ttttttcctg tttagtcact gatgcctccg tgtaaggggg atttctgttc
     4921 atgggggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt tactgatgat
     4981 gaacatgccc ggttactgga acgctgtgag ggtaaacaac tggcggtatg gatgcggcgg
     5041 gtctgcctgg gtgagccggt tgcccgttcc gggaggctgc cgacactggc accgccgtta
     5101 ctgcgtcagc tggcagccat cgggaataac ctgaaccaga cagcccgtaa ggtgaacagc
     5161 ggacagtggt cttccggcga ccgggttcag gtggtggccg cactgatggc catcggggat
     5221 gagctgcgcc ggttgcgtct ggccgtcagg gaacaggggg cgcgggatga tagttaagtt
     5281 tcatgccagg ggaaaaggtg gtggcagtgg tccggttgat tacctgctgg gaagggagcg
     5341 taaccgggaa ggggcaacgg tgcttcaggg taatccggaa gaagtccggg aactcatcga
     5401 tgccacgcca tttgcgaaga aatacacgtc cggtgttctg tcgttcgcgg agaaggagct
     5461 gccgccggga ggacgtgaaa aagtgatggc gagctttgag cgtgtactga tgcccggtct
     5521 cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag gacaagggac ggcttgagct
     5581 gaattttgtc attccgaaca tggagctgga gaccggaaaa cgcctacagc cgtactacga
     5641 ccgggcagac aggccgagaa ttgatgcctg gcagacgctg gtgaatcacc attacgggct
     5701 gcatgacccg aacgccccgg agaaccgccg gacgctgact ctccctgata acctgccgga
     5761 aacgaaacag gcgtttgctg agggcgtcac acgcgggata gatgctcttt accatgcagg
     5821 agagataaaa ggccgtcagg atgtgattca ggcgctcacg gaagcaggga ttgaagtggt
     5881 cagggtgacg cgaagcagta tcagcattgc agacccgaac ggcgggaaga atatcagact
     5941 gaaaggagca ttctatgagc aatcttttac agacgggcgc ggagttcgag aaaaagctga
     6001 aagagagagc cgaatctaca gagacaatgc tgaacgacga gttcagcaag ctcgaaaaat
     6061 ctgtaagcaa ggctgtgaca tcaaacgaga cgaaaatcaa agacgctata gccctgttca
     6121 cagcttcgac agaggaatca ctgaaaaagc accgggaagg ggtgaaagag gcgatgatgc
     6181 agcacaggaa ggacgtgtta aagctggcag ggaatacggg catgatgtta ctggggatgg
     6241 tcattttcct gtttaccgtg agttgcggga cgctctggta tcttggaggg atgatacagg
     6301 cgaacctgga agatatcagg aagcaggaag agacattgca gaaactgaac gcgaagacat
     6361 ggggcgtgga gttcgtacag gacgggaaca ggaaatttct ggtccttccg tacgggaaat
     6421 cagcagaggt gattccccat caggggaagg actgggtaca actgatggag tgacacagag
     6481 tgacggagct ggaaagacat ttgctgaacg cattagagca actgcaacag gactatatgc
     6541 agcggctgaa cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc
     6601 aacgggacaa cgcgatgctg aacgagcggg tcatgaagtt gagtcagcag gtgcagcact
     6661 tgagcgagcg gacagaacgc ttgagccagt tataggggtt ccgtgggcat ttctgcgata
     6721 acacacgcta aggaaaagtg aagttctttg gattgaagcg attagtacga agtggcctgt
     6781 atcgttcaag gggatggtca atttcgttcc acaggtaatc tccagtgagc gagatatgac
     6841 cccatggtag tggtgataca ctggacaaga gctcttccgg tataatgatc ccctggctgc
     6901 gaatgtaatc aacagcccga ctgagataga ccgtgttcca cagaattatt gcattaacaa
     6961 caaggttgag gcccgatgcc ctgtgagcca ttgtctcggc agccctgttc cggaattcgc
     7021 ccagttggtg aagaaaaata gcgcgggcaa gtgcatggcg gctttcgcct ttgttgagga
     7081 ttgcgtgtga tctgcgacgt aaccgagtgt caagcaacca gtcacagata aaaattgagc
     7141 gttcaatacg tcccatttca cgcagggcca gattgagcct gttttgtctc ggagatgctg
     7201 ccagcttttt cagtattacc gatggtggta ccagaccagc gccgatcgag gccttaagcc
     7261 gcagaacttc atcccagtgc tgttctatta cttccatttt ggcctttccg gcgatcaggg
     7321 catcaagtgg tttatattcc tctgaggggg aaataacgta caacctgcga ctgccgaggt
     7381 tgcgaatacg gggtatgagt ctgtaaccaa aagagtgaaa cattgaaaat gttgtttcag
     7441 tcgcaccggc tgtatcggtg gcatgctcat gaattaccac agaactttcg ttatgaagta
     7501 agccgtcaag tacgtaggga gcctcgcttt cagaggcctg aatcattctg gaaaaaaagg
     7561 atgcaaaacg gttcgataaa aaaccataaa tcgacgcgcc aggtcgtttg ccgtatttgg
     7621 cgttatattc gaggctggct tctcctcgtc cacccgcagg aaaaaactgc ccgtccgagg
     7681 atgacacatg cccatccccc catatagcag caaaaggatg ttcctgttgc gcatcgacca
     7741 gaactgatgt ggcagacgca taggtttcag tgcgtaaatg acgatccatc attaccatca
     7801 tctggtgaat ggtgacaccc cgggaactct gcgccatccg ttctgctccc gcattcgtcg
     7861 catcggcaag gatcgcagcc ataagtgcag cttcatcttc cgcctgttcc ccggtgcgat
     7921 aatgggtgaa acaatcaaga aaactggtcc agctatgaac ttctgccagc aaactggtaa
     7981 ttctgatgcg tggtaccagc atgtagagtt tacggctgag agccttaatt ttgtcgctct
     8041 cttcttcgcg aatcggagac acagacaacc ctctgctgga aagttctgca tcgggtatgg
     8101 cattctccga agcagctttt gccagcgctt tcagttttgc gttgagtgtg gtggttcgct
     8161 cagcccgcca ttcagcaaag ttgtccggaa tggcaagtcc cagccgggat tctgcccgca
     8221 tctgcccgaa aacatggtga ggaagaaggt agtcctcaaa actacgccag gcccgactac
     8281 catcaaccca tatgtcacct gttttcaggc gttcccgcaa atgaacgaat acggcgactt
     8341 cccacgcctg cagatcggta ttttctccat ggatgacacg acgtcgccat ttatgggaca
     8401 taaaatcgag cggaatttgt ttgggtaatt ttcggccatg atagaacgcc tgcaaatacc
     8461 caatcgccaa caaaacgcta tcatccgggc gaaaagctct gaaagaaaaa gcgctgaaaa
     8521 tgagtcgtcc aagcttacgt aatgattttt gtcgtccgat aagttcatcc agttcagaac
     8581 agtgatggga agatgccacc gaagctgcag cctccatact ggcaatcaaa ccttcccagc
     8641 cgagtgatgc tgttattaca gattcaatat cagtattacc gtcacgtgct gccaggagag
     8701 cccgtcccaa cttaatatga tcctgagcta cgacttcaag tacttcagcg gctttcagac
     8761 gacgctcttt ctggcttatc tctgctctgc gcctcgtgct gccaatcaat ttgcagaaca
     8821 tatcaattgc cagatctgta attgctgcct gcttttcaat aacaaaggct accagcgtgg
     8881 catgacagcg ttcggatgcc attcgttgta tttcacgcgc atgtaaaacg cgagaatccc
     8941 gggcaataat accgtaacgg tttacatgaa tccgtttacg ttgctcatcg gagattgccg
     9001 ctgttcgtaa tacctccagc ctggcaatta accccttaag attcttgagt ttcacgcctt
     9061 ccggcgcttc agctatccag cctaaaatgc tcttttctcc gactcgttcg gtaagcaact
     9121 gctcgagagc gctaatggat gaaggtttca gatccctgat gagttcccga taagaaaggc
     9181 gacgggcagc cgcccggccc gccattgcca gacggatgag ttgtgccgga gcaggggcaa
     9241 gtaacttctg gtccttcagc ttattgataa ctgctctggt gatcggcagt cctttttcgg
     9301 tggttgctgc ttcgcgtgta gccgcgataa tgagcattct gtaatcgcta ctccggacgg
     9361 aacgaagctc caaagccgaa actatttcgc gggcatgttc ccggcgggtt tcctcacgtt
     9421 gtgcgtaaag tgtaaaaaca gcagggtcaa tatccagttg ttccgcaaca acggtgataa
     9481 cagcctccgg gggaatctct cctgcacgca gtgtacgacc gagatcacgg acaagtgcaa
     9541 gctgaatggc aaaacccaga cggttatgag atcgacgatg agttctggcg aatgcaatat
     9601 cctcgcagga gagcgaaaac cggggaagtg catcctcata aagttcaggt aatccaaaaa
     9661 gcgtatcgta cgatttagag tcgagaaatg acattgctct gatccgttgt cagattacgc
     9721 cccccaggtc cggcccagcc aattcgtcgt agagtatcgg tgagcgttga gcgtggcaca
     9781 ctgaatgcgc gacatactga cgctttactg gcacctcctt ccagagctgc gaggatttga
     9841 tcaacctttt cagcatctat cgccggtggc cttccgcctt ttctcccccg ccggagagca
     9901 gcagccagac cagcattaac gcgttctgtg ataagtgttc tttcgtactc cgcgagcgtg
     9961 ccaaaaaggt taaagaggaa cgagccgtgt ggcgttgttg tatcaataga ttcagtcaat
    10021 gatcggaaag caataccacg aattttcagg tcttcaataa ttcggagcaa atgtgagagt
    10081 gaacgaccaa gtcgatccag tttccagact atcagtacat caccttcgcg aagttcagcg
    10141 agacaggact taagtcccgg gcgattatca cgagcacctg aagcatgatc ctgatgtaaa
    10201 tgccggggat ccactccagc agcaatcaac gcatcacgct gcaaagaaac tgactgacgt
    10261 tcatcattgc ttgatacccg catataacca atcaacatgt acgacaaacc ttaaaattga
    10321 agtttccgta cagtttctca ttccgacagg gtttgtcacg caaaaaatca cagatttatg
    10381 gattcaattt tcttcaactg caggacctga cggaaaacaa gtgatttccg tcagggagga
    10441 atcgcttagc gggtgttatc gcagaaattc ccacggaacc ccttatacag cgagaacagg
    10501 agataaggga cgaacggctg atacaggaac gcgaacatga gttatccctg gagcgtgagc
    10561 gccagctgga aatacaggaa cggacactgg atggcccttc gctgggatgg taatcagaga
    10621 caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt gggataatgg ttggttcggg
    10681 g
//
