LOCUS       NZ_CP148343            10981 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0422 plasmid pZ1323HEC0422-5,
            complete sequence.
ACCESSION   NZ_CP148343
VERSION     NZ_CP148343.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441600
            Assembly: GCF_037543915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037543915.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:17:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,593
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,136
            CDSs (with protein)               :: 5,136
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 331
            CDSs (without protein)            :: 331
            Pseudo Genes (ambiguous residues) :: 0 of 331
            Pseudo Genes (frameshifted)       :: 121 of 331
            Pseudo Genes (incomplete)         :: 209 of 331
            Pseudo Genes (internal stop)      :: 62 of 331
            Pseudo Genes (multiple problems)  :: 55 of 331
            Pseudo Genes (short protein)      :: 3 of 331
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148343.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0422"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0422-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-18"
     gene            complement(437..643)
                     /locus_tag="WHU23_RS27790"
                     /old_locus_tag="WHU23_27795"
     CDS             complement(437..643)
                     /locus_tag="WHU23_RS27790"
                     /old_locus_tag="WHU23_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_027663146.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGELMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(636..1019)
                     /locus_tag="WHU23_RS27795"
                     /old_locus_tag="WHU23_27800"
     CDS             complement(636..1019)
                     /locus_tag="WHU23_RS27795"
                     /old_locus_tag="WHU23_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_027663147.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKVVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(1624..2685)
                     /locus_tag="WHU23_RS27800"
                     /old_locus_tag="WHU23_27805"
     CDS             complement(1624..2685)
                     /locus_tag="WHU23_RS27800"
                     /old_locus_tag="WHU23_27805"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_069201238.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     KNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            2773..3930
                     /locus_tag="WHU23_RS27805"
                     /old_locus_tag="WHU23_27810"
     CDS             2773..3930
                     /locus_tag="WHU23_RS27805"
                     /old_locus_tag="WHU23_27810"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4158..4316)
                     /locus_tag="WHU23_RS27810"
                     /old_locus_tag="WHU23_27815"
     CDS             complement(4158..4316)
                     /locus_tag="WHU23_RS27810"
                     /old_locus_tag="WHU23_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(4977..5168)
                     /locus_tag="WHU23_RS27815"
                     /old_locus_tag="WHU23_27820"
     CDS             complement(4977..5168)
                     /locus_tag="WHU23_RS27815"
                     /old_locus_tag="WHU23_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5233..5556
                     /locus_tag="WHU23_RS27820"
                     /old_locus_tag="WHU23_27825"
     CDS             5233..5556
                     /locus_tag="WHU23_RS27820"
                     /old_locus_tag="WHU23_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_000955995.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            5546..>5779
                     /locus_tag="WHU23_RS27825"
                     /old_locus_tag="WHU23_27830"
                     /pseudo=""
     CDS             5546..>5779
                     /locus_tag="WHU23_RS27825"
                     /old_locus_tag="WHU23_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            5963..6586
                     /locus_tag="WHU23_RS27830"
                     /old_locus_tag="WHU23_27835"
     CDS             5963..6586
                     /locus_tag="WHU23_RS27830"
                     /old_locus_tag="WHU23_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_077150956.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRL"
                     /name="recombinase family protein"
     gene            6567..9533
                     /locus_tag="WHU23_RS27835"
                     /old_locus_tag="WHU23_27840"
     CDS             6567..9533
                     /locus_tag="WHU23_RS27835"
                     /old_locus_tag="WHU23_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_072024409.1"
                     /translation="MSFLDSKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFR
                     AFRPDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVH
                     LRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTL
                     NAKLKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLL
                     AEVHSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVM
                     MDRHLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKY
                     GKRPGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATE
                     TTFSMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLR
                     LKASIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHA
                     ILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDY
                     IRSQGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     gene            9582..>9989
                     /locus_tag="WHU23_RS27840"
                     /old_locus_tag="WHU23_27845"
                     /pseudo=""
     CDS             9582..>9989
                     /locus_tag="WHU23_RS27840"
                     /old_locus_tag="WHU23_27845"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10027..10545
                     /locus_tag="WHU23_RS27845"
                     /old_locus_tag="WHU23_27850"
     CDS             10027..10545
                     /locus_tag="WHU23_RS27845"
                     /old_locus_tag="WHU23_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_096933969.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIRDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            10552..10806
                     /locus_tag="WHU23_RS27850"
                     /old_locus_tag="WHU23_27855"
     CDS             10552..10806
                     /locus_tag="WHU23_RS27850"
                     /old_locus_tag="WHU23_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_161399083.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQQDNA
                     MLNEQVMQLSQQVQHLSERTERLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4815..4899
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          complement(4245..4795)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.404"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5204..5553
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="99.0"
                     /evalue="1.1e-63"
     Typing          9568..10910
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.848"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaccgggaa cgcgcttcag tcagaccttg ctgaaaacac aggaacgcgt taaaacgcat
       61 acgggcgcga ttctggtgtg tcttttgttg ctgtgttttg cctctattgc cagatcgcgc
      121 tcattcccct gaacttctgt tttcccgctc cggtccccgt cttcttcgct tccgaaagca
      181 tgaaaaaaat ccgtttcagc agtctctggc tggcaattcc tgcgtgattc acatggctgc
      241 atagctatgc atgtggttaa aatttaccgg gtgcgatcgc ggcagttttt cgggtggttt
      301 gttgccattt ttacctgtct gctgccgtga tcgcaccgaa cgcgtttctg cggtgcgtgc
      361 aacggatgtt atggtaaata caatatgcag ccagtcttag agacgaaaag tccgcccaga
      421 agactgactt ttcagatcaa ccttcagcat ctaacttgtc tatcgctttt ttattgcgcc
      481 gataggtagc cattagttca ccatcgcctg attgtaagtc acgacctgct tgctcagcat
      541 aggtatcgtt tacagtctta gcgccttttt tataggcctt tttaacatca tcaaatccgg
      601 cttggatgac ttctttcggc ttttcttgag gtaatttagc catgctgtga atcctctata
      661 aggttgatta tttttccaac agataagacg gcatcttctg tgtcgctgat tttatgattt
      721 atccaaccgt cctctatctt taatttgttc actactccta atgtaaaaac aacctttttt
      781 tcaggaaaga aaattgcaaa ggttctactt ttctctttcc aatcagaggg cggcgtcgaa
      841 cggaacgcta cattcctcga tttaagtatc gctttgattt tactaattaa ttcttttttt
      901 tgtttattgc tgagattttt gacgaaccct ttgctgagat tgtctgtgta atcatcaaaa
      961 gaaatatcaa ctggtttgtt tttttgtttt ttatcagata gatgaacaat gtttgacact
     1021 ggatatcctg ctttttgatg aatgaatact cagaagagta gcggaactgt cttgttagtc
     1081 acagaggtag agtatcaatt ctacgataga tgggttcgaa gatccggctg agaccgttca
     1141 gtacgcggtc gagatgttat gttggggtct gaagtccaat ggaacgaaaa cgtacgttag
     1201 tgaagtaact gtatgatata tcaagacata atagacatag aaaaacgcca tcaaaacggt
     1261 gtctttttaa tcgaaaattg gcacttaacg gacggcattg tctactaatc gatttttaag
     1321 acgataattt tcctcttacc gtacattttc gtacggttgg ccctcaaacc ccatgttcaa
     1381 tgtaatgacc gtcgaaattg cctttacggg catgaagcaa ccaggagcaa tcattggcaa
     1441 aggtaactag agttcaatgc atggtaatca tttttgagct tgtgatgcat agagttggtt
     1501 gctgttccat tgcgtctcaa actccgtcaa gatgctcgcc gtaaaagcga gcatgtctag
     1561 tatgcctgtt ccgctactga tttttgatgg gcgaattcag ggctggcgag ccaccaagaa
     1621 aaattacttt atttcatcaa acaggtgaga tgcagtataa taaacgcttt gatcaaagcg
     1681 tctctctggg cttaatttat tttgcccgcc atttaaaatt atttctttta atactgatgg
     1741 tgggaggatg ggttttgttc ttcttgggtg atccatataa gcttttgtta tttttccgtt
     1801 tagcgagaac tctttatcac tttgatatcc aaagttcatc tcagtataaa ttctattgat
     1861 gtttgtcttg tttttgctcg caatttcata caccattctg gcgagtctgg ctgatgatct
     1921 cattagtctg ctggcagatg aattgttata tgaggatcta tcaataaagg ttcgacacaa
     1981 agcaaaatca ctccagatga atatgtcaaa ggcattttga tctaatattg ggctcttgcc
     2041 ttttgttttc cataatggtt gcattaaaaa aggtttctgg taatcaagaa aattttcttg
     2101 gaaagtgttt aggcaattta atatattttc tttctttgaa agaacttcag tttcattccc
     2161 ccagtgctgt atgctttcgc atgtggattc aaatattttt cttatttcat ggaatttatc
     2221 gctgcaagag tgcattatgc caagggcaca ataagaagtg cttgcaggtc ttattacaat
     2281 ctctgaactc cattcgctct cgttttcttt atatgtcgtg ctatctggaa gtacggttaa
     2341 ttttatttct agtgctttta aaaaactatt ttttcctttt ataactagat ctattccttt
     2401 tatgtcatca taagcatact gttggtatgg ttcatatttt ttctcaaatt gaaattcaag
     2461 atcactattt tttaatgttg tgttaaaaac gaagtcaaaa gatacttctt tggcatgcac
     2521 ttttaaatcc ttatctaaat aaagataaat cggattcatt ttgttgtctc tcatataaca
     2581 tgctagagag gtgggaaaag aactgttaaa ttggttcttt ccccacagat cgctctcttt
     2641 cctgttggtg ttttttaaac cataaagaga ttctattttg ctcacttttt gctcctgacg
     2701 tcaatttatt gacgtctatg gtgtgtttct atatcatgat gtggagtgat atgataatga
     2761 gttttttatg ttatgagaat aattgatctt tttagtgggt gtggggggtt atccctaggc
     2821 tttttgaagg gggggtttga tgttgtaggt gcttatgatt tctgggatcc tgctattgaa
     2881 tgttacagag ataattttag ccatccgata aaaaaactag atttgagcaa tgttgatgat
     2941 gtggtgaggg agcttaaaga tatagacttc gatatgatta ttggagggcc tccttgccaa
     3001 gacttcagtc atgcaggttt aagaattgaa ggtgctagag ctaacttaac tcgctcattt
     3061 tcagaaatta taaaaagaat aaagccaaag tggtttgtga tggaaaatgt agatcgggca
     3121 ttaagaagtg gtgcctattt agaagctcga ggaatcttta aagaatcagg gtatgggtta
     3181 acagaaattg ttttggatgc cagtaagtgt ggtgttccgc aaaaaagaaa aagattattt
     3241 gtaattggaa agcttgacgt gagggatggt tttatcctta acgaagtgat gtgcgggata
     3301 tcgaaagata gtatgacagt tagaaattat cttggtgatt cattggggat tgagtattat
     3361 tacagacatc cgagaaatta taacagaagg gctattttct ctattgatga acctgcgcca
     3421 acagtaagag gggttaatag accgatacct gatggatatt tagggcatgc aggcgatcct
     3481 gtcagtattt ctgagaatgt aaggccatta acaacctttg aaagagcaag attgcaaaca
     3541 tttcctgaag actttaaatt taaaggtgca aaaacaaatt tggagcaaat gataggtaat
     3601 gctgttccgg ttgagttagc aaaatatgtt gcggtgacaa tcatggagta tgaaaaaaaa
     3661 caagtaaaag gtatttatga taaagaaggc tttagggcgt ggttgctgaa tgaaaagaaa
     3721 ctaacaaaaa gaacctctag cgatattata tctagatgtt gcagaggtgt ttcatttttt
     3781 gattcagagg gggtggattt ttataactgt gaaatagatg aaattattat gaaactggaa
     3841 aggcttgaat cgtttgtcag gcttggggtt agcctgaagt ctcagttaag aagggctttt
     3901 aaattatatt acgaatattg tcgaaggtaa taaatacgaa tttatgtctc tcaggggagg
     3961 gggaggggat aaaaagatca aaggatcctc ttgtgatcct ttttttctgc gcgtaatctg
     4021 ctgcctgtaa acaaaaaaac caccgctacc aacggtggtt tgtttgccgg aatctagagc
     4081 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatactgtcc
     4141 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     4201 gtccagttac cagtggctgc tgccagtggc gctttgtcgt gtcttaccgg gttggactca
     4261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     4381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4501 gggtttcgcc acctctggct tgagcgtcga tttctgtgat gctcgtcagg ggggcggagc
     4561 ctatggaaaa acgccagcgg cgcggccttt tgttgtcctt tgcctttttg ctcacatgtt
     4621 ctttccggtc ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagccga
     4681 ctccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     4741 gcgccggatg cggtattttc tccttacgca ccatatgcgg tatttcacac cgcatatggt
     4801 gctcttttct tataatctgc actgatgcca catagttaag ccagtataca ctccgctatc
     4861 gctacgtgac tggttcaggg cttcgccccg aaaccccctg acgcgccctg acgggcttgt
     4921 ctgctcccgg catccgctca cagacaagct gtgaccgtct ccgggagctg tatgtgtcag
     4981 aggttttcac cgtcatcccc gaagcgtgcg aggcagctgc ggtaaagctc atcggcgtgg
     5041 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagttcgtt gagcttttcc
     5101 agcagcgtta atgtctggct tcttataaag cgggccatgt taagggcggt tttttcctgt
     5161 ttagtcactg atgcctccgt gtaagggggg atttctgttc atggggtaat gataccgatg
     5221 aaacgcgaga ggatgctcac gatacgggtt actgatgatg aacatgcacg gttactggaa
     5281 cgctgtgagg gtaaacagct ggcggtatgg atgcggcggg tctgtctggg tgagccggtt
     5341 gcccgttccg ggaagctgcc gacactggca ccgccgttac tgcgccagct ggctgccatc
     5401 gggaataacc tgaatcagac ggcgaggaag gtgaacagcg ggcagtggtc ttccggtgac
     5461 cgggttcagg tggtggccgc actgatggcc atcgagagag agttgcgcag cttacggcag
     5521 gtcgtcaggg aacatggagc gcgggatgat agttaagttt catgccaggg gaaaaggcgg
     5581 tggcagtggt ccggttgatt acctgctggg aagggagcgt aaccgggaag gggcaacggt
     5641 gcttcagggt gatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa
     5701 atacacgtca ggtgttctgt cgttcgcgga gaaggagctg ccgccgggag gacgtgaaaa
     5761 ggtgatggcg agctttgagg ggttccgtgg gaatttctgc gataacaccc gctaagcgat
     5821 tcctccctga cggaaatcac ttgttttccg tcaggtcctg cagttgaaga aaattgaatc
     5881 cataaatctg tgattttttg cgtgacaaac cctgtcggaa tgagaaactg tacggaaact
     5941 tcaattttaa ggtttgtcgt acatgttgat tggttatatg cgggtatcaa gcaatgatga
     6001 acgtcagtca gtttctttgc agcgtgatgc gttgattgct gctggagtgg atccccggca
     6061 tttacatcag gatcatgctt caggtgctcg tgataatcgc ccgggactta agtcctgtct
     6121 cgctgaactt cgcgaaggtg atgtactgat agtctggaaa ctggatcgac ttggtcgttc
     6181 actctcacat ttgctccgaa ttattgaaga cctgaaaatt cgtggtattg ctttccgatc
     6241 attgactgaa tctattgata caacaacgcc acacggctcg ttcctcttta acctttttgg
     6301 cacgctcgcg gagtacgaaa gaacacttat cacagaacgc gttaatgctg gtctggctgc
     6361 tgctctccgg cgggggagaa aaggcggaag gccaccggcg atagatgctg aaaaggttga
     6421 tcaaatcctc gcagctctgg aaggaggtgc cagtaaagcg tcagtatgtc gcgcattcag
     6481 tgtgccacgc tcaacgctca ccgatactct acgacgaatt ggctgggccg gacctggggg
     6541 gcgtaatctg acaacggatc agagcaatgt catttctcga ctctaaatcg tacgatacgc
     6601 tttttggatt acctgaactt tatgaggatg cacttccccg gttttcgctc tcctgcgagg
     6661 atattgcatt cgccagaact catcgtcgat ctcataaccg tctgggtttt gccattcagc
     6721 ttgcacttgt ccgtgatctc ggtcgtacac tgcgtgcagg agagattccc ccggaggctg
     6781 ttatcaccgt tgttgcggaa caactggata ttgaccctgc tgtttttaca ctttacgcac
     6841 aacgtgagga aacccgccgg gaacatgccc gcgaaatagt ttcggctttg gagcttcgtt
     6901 ccgtccggag tagcgattac agaatgctca ttatcgcggc tacacgcgaa gcagcaacca
     6961 ccgaaaaagg actgccgatc accagagcag ttatcaataa gctgaaggac cagaagttac
     7021 ttgcccctgc tccggcacaa ctcatccgtc tggcaatggc gggccgggcg gctgcccgtc
     7081 gcctttctta tcgggaactc atcagggatc tgaaaccttc atccattagc gctctcgagc
     7141 agttgcttac cgaacgagtc ggagaaaaga gcattttagg ctggatagct gaagcgccgg
     7201 aaggcgtgaa actcaagaat cttaaggggt taattgccag gctggaggta ttacgaacag
     7261 cggcaatctc cgatgagcaa cgtaaacgga ttcatgtaaa ccgttacggt attattgccc
     7321 gggattctcg cgttttacat gcgcgtgaaa tacaacgaat ggcatccgaa cgctgtcatg
     7381 ccacgctggt agcctttgtt attgaaaagc aggcagcaat tacagatctg gcaattgata
     7441 tgttctgcaa attgattggc agcacgaggc gcagagcaga gataagccag aaagagcgtc
     7501 gtctgaaagc cgctgaagta cttgaagtcg tagctcagga tcatattaag ttgggacggg
     7561 ctctcctggc agcacgtgac ggtaatactg atattgaatc tgtaataaca gcatcactcg
     7621 gctgggaagg tttgattgcc agtatggagg ctgcagcttc ggtggcatct tcccatcact
     7681 gttctgaact ggatgaactt atcggacgac aaaaatcatt acgtaagctt ggacgactca
     7741 ttttcagcgc tttttctttc agagcttttc gcccggatga tagcgttttg ttggcgattg
     7801 ggtatttgca ggcgttctat catggccgaa aattacccaa acaaattccg ctcgatttta
     7861 tgtcccataa atggcgacgt cgtgtcatcc atggagaaaa taccgatctg caggcgtggg
     7921 aagtcgccgt attcgttcat ttgcgggaac gcctgaaaac aggtgacata tgggttgatg
     7981 gtagtcgggc ctggcgtagt tttgaggact accttcttcc tcaccatgtt ttcgggcaga
     8041 tgcgggcaga atcccggctg ggacttgcca ttccggacaa ctttgctgaa tggcgggctg
     8101 agcgaaccac cacactcaac gcaaaactga aagcgctggc aaaagctgct tcggagaatg
     8161 ccatacccga tgcagaactt tccagcagag ggttgtctgt gtctccgatt cgcgaagaag
     8221 agagcgacaa aattaaggct ctcagccgta aactctacat gctggtacca cgcatcagaa
     8281 ttaccagttt gctggcagaa gttcatagct ggaccagttt tcttgattgt ttcacccatt
     8341 atcgcaccgg ggaacaggcg gaagatgaag ctgcacttat ggctgcgatc cttgccgatg
     8401 cgacgaatgc gggagcagaa cggatggcgc agagttcccg gggtgtcacc attcaccaga
     8461 tgatggtaat gatggatcgt catttacgca ctgaaaccta tgcgtctgcc acatcagttc
     8521 tggtcgatgc gcaacaggaa catccttttg ctgctatatg gggggatggg catgtgtcat
     8581 cctcggacgg gcagtttttt cctgcgggtg gacgaggaga agccagcctc gaatataacg
     8641 ccaaatacgg caaacgacct ggcgcgtcga tttatggttt tttatcgaac cgttttgcat
     8701 cctttttttc cagaatgatt caggcctctg aaagcgaggc tccctacgta cttgacggct
     8761 tacttcataa cgaaagttct gtggtaattc atgagcatgc caccgataca gccggtgcga
     8821 ctgaaacaac attttcaatg tttcactctt ttggttacag actcataccc cgtattcgca
     8881 acctcggcag tcgcaggttg tacgttattt ccccctcaga ggaatataaa ccacttgatg
     8941 ccctgatcgc cggaaaggcc aaaatggaag taatagaaca gcactgggat gaagttctgc
     9001 ggcttaaggc ctcgatcggc gctggtctgg taccaccatc ggtaatactg aaaaagctgg
     9061 cagcatctcc gagacaaaac aggctcaatc tggccctgcg tgaaatggga cgtattgaac
     9121 gctcaatttt tatctgtgac tggttgcttg acactcggtt acgtcgcaga tcacacgcaa
     9181 tcctcaacaa aggcgaaagc cgccatgcac ttgcccgcgc tatttttctt caccaactgg
     9241 gcgaattccg gaacagggct gccgagacaa tggctcacag ggcatcgggc ctcaaccttg
     9301 ttgttaatgc aataattctg tggaacacgg tctatctcag tcgggctgtt gattacattc
     9361 gcagccaggg gatcattata ccggaagagc tcttgtccag tgtatcacca ctaccatggg
     9421 gtcatatctc gctcactgga gattacctgt ggaacgaaat tgaccatccc cttgaacgat
     9481 acaggccact tcgtactaat cgcttcaatc caaagaactt cacttttcct tagcgtgtgt
     9541 tatcgcagaa atgcccacgg aacccctttg agcgtgtact gatgcccggt ctcgaaaaga
     9601 atcagtacag catcctgtgg gtagagcacc aggacaaggg acggcttgag ctgaattttg
     9661 tcattccgaa catggagctg gagaccggaa aacgcctcca gccgtactac gaccgggcag
     9721 acaggccgag aattgatgcc tggcagacgc tggtgaatca ccattacggg ctgcatgacc
     9781 ccaacgcccc ggagaaccgc cggacgctga cccttcctga taacctgccg gaaacgaaac
     9841 aggcgcttgc agagagcgtc acacgcggga tagacgctct ttatcatgtc ggggagataa
     9901 aaggccgtca ggatgtgatt caggcgctca cagaggcagg gcttgaagtg gtcagggtga
     9961 cgcgaagcag tatcagcatt gcagacccga acggtgggaa gaatatcagg ctgaaaggag
    10021 cattttatga gcaatctttt acagacgggc gcggagttcg agaaaaagct gaaagagaga
    10081 gccgaatcta cagagacaat gctgaacgac gagttcagca agctcgaaaa atctgtaagc
    10141 aaggctgtga catcaaacga gacgaaaatc agagacgcta tagccctgtt cacagcttcg
    10201 acagaggaat cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg
    10261 aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat ggtcattttc
    10321 ctgtttaccg tgagtggcgg gacgctctgg tatcttggcg ggatgataca ggcgaacctg
    10381 gaagaaatca ggatacagga agaaacattg cagaaactga acgcgaagac atggggcgtg
    10441 gagtttgtgc aggacgggag aaggaaattt ctggtcatcc cgcaggggaa atcggcaacg
    10501 gtgattccct atcaggggaa ggactgggta caactgacgg agtgacacag agtgacggag
    10561 ctggaaagac atttgctgaa cgccttagag caactgcaac aggactatat gcagcggctg
    10621 aacgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacaggac
    10681 aacgcgatgc tgaacgagca ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag
    10741 cggacagaac gcttgagcga gcggacagaa cgcttgagcc agttatacag cgagaacagg
    10801 agataaggga cgaacggctg atacaggagc gcgaacatga gttatccctg gagcgtgagc
    10861 gccagctgga aatacaggaa cggacactgg atggcccttc gctgggatgg taatcagaga
    10921 caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt gggataatgg ttggttcggg
    10981 g
//
