LOCUS       NZ_CP148336             4072 bp    DNA     circular CON 15-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0423 plasmid pZ1323HEC0423-3,
            complete sequence.
ACCESSION   NZ_CP148336
VERSION     NZ_CP148336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441601
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.9
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 127X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_038096605.1-RS_2024_04_15
            Annotation Date              :: 04/15/2024 11:24:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148336.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0423"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0423-3"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-20"
     gene            9..701
                     /locus_tag="WHU62_RS00005"
                     /old_locus_tag="WHU62_25265"
     CDS             9..701
                     /locus_tag="WHU62_RS00005"
                     /old_locus_tag="WHU62_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(957..3116)
                     /gene="mobQ"
                     /locus_tag="WHU62_RS00010"
                     /old_locus_tag="WHU62_25270"
     CDS             complement(957..3116)
                     /gene="mobQ"
                     /locus_tag="WHU62_RS00010"
                     /old_locus_tag="WHU62_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3295..3549
                     /gene="mobC"
                     /locus_tag="WHU62_RS00015"
                     /old_locus_tag="WHU62_25275"
     CDS             3295..3549
                     /gene="mobC"
                     /locus_tag="WHU62_RS00015"
                     /old_locus_tag="WHU62_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2759..3117)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3118..3294
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          10..701
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(961..3203)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atctttgtat gtcggacttg attgcctaca agtcgaatgc tttggttgag gccagctaca
       61 agttgaccct acaggaacaa cgttttctgt tgctgtgtat cagtcgattg aaatctggtg
      121 ctgatgctga attgcaaaag acaatgacca ttaccgctgc ggaatatttt gattcattcc
      181 ctgacatggg gcggaaaaat gccgaggttc agttacagga agcgatcgat cgtttatggg
      241 acaggtcgat cattctcaag aatgacgaga agcgtgagga gttccgctgg atccagtatc
      301 gggcacagta cgctaaaggt gaggccaagg ctcgaataac cttttctgac gcggtaatgc
      361 cttatctgac gcaactgaag gggcaattca cgcgcgttgt tataaaaaac atatcaggtt
      421 tatcaagtag ttacagtata agaatatacg agttcctcca gcaattccgc agtacgggag
      481 agagaattat tgctcttaat gactttcgtt caatgttagg gattgaaaat aaatacaaac
      541 aattcagaga cttaaataaa atactaataa aaccctgtgt tgacgaactg aacaaaaaaa
      601 gcgacctggc tgtaacggtt gagacgatca aaaaggggcg cacggttgta gccctgcatt
      661 ttcgcttcaa ggaagacaaa cagatcaaga tgacgatcta aaatcgattt taaggcacct
      721 aaaacgctcc ctgctgcgtt ttttttgtgt tagtagtgcg aaggtactat tgaagtgaaa
      781 aattgcgtca aaagcgctct aatgcagcga aaaaagaacc cccaattttt gggggcggaa
      841 aaacaaaact atgcttgcat tatgtgtgtg cttcttgcat tcatccctgt cgatgaacat
      901 agaagaacat cgacgaacat aacacgatgt taaacaaaac aaaatattgt tcacattcac
      961 tgtgaccaac cgcgatcatg tctcctctcg gtggtgtgtg ttcttgcatc ttcctcgcgc
     1021 cgccattcat cccaggtacg attgaaaccc agagagcgga acagatgatc cggctgtttt
     1081 tctggtgtgt tgacggcagc aaaaagcggt gcctcacggg ttttcagatg aaactccata
     1141 agttccggcg caggtgatat gtggctgtct gcatcgtgat ttacggcaac accataccct
     1201 gcataccgta accccttatc ggtcagtgca ctgatgacca gctggctgtt atcggctgag
     1261 ggaacggcaa acgcgggata cagcgtacca ttaagccgga caacaccgcc cagagagtca
     1321 tccacgccca tgaccagcaa aagctgaagc tggcgggttt cccggtcgac agaactcatt
     1381 accggcatca gcgtcggtag cagacgtccc tgcgtaaaat ccaccttcct tgctgccagt
     1441 gatggcatgg ctggctggcg gtcttctcca agacagaaag actccggcca tgtcttcggc
     1501 tggcggagat tgcgggtata ttgcggccat gtctggcaaa cctgcgtcag aagttgctca
     1561 aaggtcgcag aggaataaac acgttgcagt ggtggatgtt gaccgatttc gcttcggtca
     1621 cagatataac cactcctgtc acgcattaaa tcaacacgct caaagattct ggtgcggtat
     1681 ttctccagct cgttccccca gagcgtgtat tctccgttcc ggttgtgaag cagaaggtaa
     1741 caggtcattt ccccgttacc aggcatgggg gcctcgccat accccgtaac gactgcgctc
     1801 agtacccctg aatccataac ggcattgtca tttttcctga gctgttccgc tcgtcgctcc
     1861 gcgcgactac gttctgtatc ctcctgctcc ttccggcgca attcctcttc acgtttaagc
     1921 aactggactc tgagagctgc aaggccggat gccccccact gctgccactg tttttcaaat
     1981 gccgtctgac tcgcgggaat gtcctgtttc tccaccttcc ggtaatgctg tcgcaatttt
     2041 tccatttgct ggctcagcga ctggattttt ccctgttcgc tacggtattc cggtgccgcc
     2101 cattgcatca ggggaatacg ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg
     2161 gcagcatggc tcatcgcttc ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc
     2221 gcctgcatat ctgccatcat cgacatggca ttactggtga gtttccatgc cgcattactg
     2281 tccttcggca acgcttcgat gatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc
     2341 acgatattca gaaggtcagc cacacggcgg gcaggctctg tcagcccgct gttcctgagt
     2401 tcagtgcggg aaatctgtat ttccaggtta agcctgttaa tcagacgatt ctgttcgccg
     2461 cgttctgttt cgataccgcg tgtttccatc gcacaggcag actttccgag gtgaatacca
     2521 ggcgttttct caagcccctg tcgctcgtat gaacgatggt ctatctcttc ctgataaccg
     2581 gcgttcgcca gtgctctgtt tgcatggtca gcccatgatg cacgccacgt ctcggcatgt
     2641 gtccggtcgt tccagtccct gacctttccg ccgaacccag caggtccgac agccctcgtg
     2701 gtcagcatga tgtgcgcatg gggatttgtt ttatccatgt gatggaacgc cacatcagca
     2761 atcatgccct gactgacaaa gttttctctg acaaaggcga gaacggtttc ccgtgcggca
     2821 tcctgcggca gttcacgggg gatagcgagt tcaatttccc gcgcaagttg tgagttcttc
     2881 cggcattcgg ccttctcaac ggcattccag agttcggcac ggttcgtaca ccaggcaggg
     2941 gctgtcgcgg gggtcagaat cacagcttca gccacaccgc ttttgcgggt gtagtcgtga
     3001 gtcagtccgg tacgctcatc aagcatcagt gaaccggaac gataggcagc ggaagctacg
     3061 gcgctgtagc cgctgcttct ggagataatt ttcatgctga gatgataaat cgccatttct
     3121 gccgtaatgc ctttttgtag ttttctttca gccccgcagg gcgcacacaa tcgaaacgga
     3181 gtggagattg tataagtgcg cactttgtga tttgtcgcgc attatgccag ctcagttttt
     3241 aaactaccag tgacgcgcta aaatgcgatt tttcatcgcg cacggagtta catcatgagc
     3301 agaacgctcg aacagaagat tgctgatgct gaggcgagat tacagcgcct gaaggcgaag
     3361 agccggagcc tggacacagc gcagaaggtt gttgtgggtg cagcattgct ggcaaaggtc
     3421 agaaagccgg aggaggtgca gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg
     3481 acacgacagg ctgatgtgac gcgcatactg ccgctgatta acgagctgga ggcgctgcca
     3541 ggacagtgat tgtatcgtcc gctcgccgca gccccagcga ccgagcgtta gcgagcgagt
     3601 gggcgaggaa gcggaagtga atccgtcgcc aggttgagca catacgcacg gtttttcggt
     3661 aggtgatatg cgctgaaccc gcatgaatac tggatttcca cagggttgga aggggagtat
     3721 tcggaacttg caattctgct ttcttgcaac acattgtttt tattggtttt ttgttttcag
     3781 cgccccaagt tatccacagg ctcgccgtta agtttttaga tcttgttttt aagatcttgt
     3841 ttttaatact tgttttaggg tcaaaattca ttaataaaat caatatgtta tagaggtata
     3901 tgtggaggaa tttaaggtaa aggtggagga atttaaggtg tttaggtgga ggaatttaag
     3961 gtaaaggtgg aggaatttaa ggtgttattc acagaaatgt gaggatataa aaaaaaatat
     4021 tccacatttc ttatttgtgg ggcacattaa agcatcacca catttgaacg gg
//
