LOCUS       NZ_CP148322             4072 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323HEC0494 plasmid pZ1323HEC0494-4,
            complete sequence.
ACCESSION   NZ_CP148322
VERSION     NZ_CP148322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441605
            Assembly: GCF_037541525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037541525.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:23:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,627
            CDSs (total)                      :: 5,498
            Genes (coding)                    :: 5,204
            CDSs (with protein)               :: 5,204
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 110 of 294
            Pseudo Genes (incomplete)         :: 165 of 294
            Pseudo Genes (internal stop)      :: 67 of 294
            Pseudo Genes (multiple problems)  :: 41 of 294
            Pseudo Genes (short protein)      :: 4 of 294
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323HEC0494"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323HEC0494-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-05-22"
     gene            complement(523..777)
                     /gene="mobC"
                     /locus_tag="WHU44_RS28125"
                     /old_locus_tag="WHU44_28125"
     CDS             complement(523..777)
                     /gene="mobC"
                     /locus_tag="WHU44_RS28125"
                     /old_locus_tag="WHU44_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            956..3103
                     /gene="mobQ"
                     /locus_tag="WHU44_RS28130"
                     /old_locus_tag="WHU44_28130"
     CDS             956..3103
                     /gene="mobQ"
                     /locus_tag="WHU44_RS28130"
                     /old_locus_tag="WHU44_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3361..4062)
                     /locus_tag="WHU44_RS28135"
                     /old_locus_tag="WHU44_28135"
     CDS             complement(3361..4062)
                     /locus_tag="WHU44_RS28135"
                     /old_locus_tag="WHU44_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(779..955)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          956..1314
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3362..4062)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          870..3100
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgttgctatt ggtggcgttt ttttactgta cgccacttat catatgggtg gaagtttttt
       61 ttttgcaacc ccctttttct gtggattaca cccccaaaag ctccaccttt cacccccaaa
      121 agctccacct ttcaccccca aaagctccac ctttcacccc caaaagctcc acttatcttg
      181 ccagaacatc tttaaaatca tggtgttaca agacctaaaa aggttttaaa aaaaagatct
      241 aaaaataaga tcttaaaaac ttaacggcga gcctgtggat aacttggggt gctgaaaaca
      301 aaaaaccaat aaaaacaatg cgttgaaata aagcagaatt gcaagtttcg aatacacccc
      361 ctccaaccct gtggaaaccc agtattcatg cgggttcagc gcatatcacc tgccgaaaaa
      421 ccgtgcgtat gtgctcaacc tggcgactga ttcacttccg cttcctcgcc cactcgctcg
      481 ctaacgctcg gtcgctgggg ctgcggcgag cggacgatac aatcactgtt ctggcagcgc
      541 ctccagctcg ttaatcagcg gcagtatgcg cgtcacatca gcctgtcgtg tcacctctgc
      601 cttcaggaac tggagtaacc aggcacgcaa ctgcacctcc tccggctttc tgacctttgc
      661 cagcaatgca gcacccacaa caaccttctg cgctgtgtcc aggctccggc tcttcgcctt
      721 caggcgctgt aatctcgcct cagcatcagc aatcttctgt tcgagcgttc tgctcatgat
      781 gtaactccgt gcgcgatgaa aaatcgcatt ttagcgcgtc actggtagtt taaaaactga
      841 gctggcataa tgcacggcaa atcacgaagt gcgcacttat acaatctcca ctccgtttcg
      901 attgtgtgcg ccctgcgggg ctgaaagaaa actacaaaaa ggcattacgg cagaaatggc
      961 gatttatcat ctcagcatga aaattatctc cagaagcagc ggctacagcg ccgtagcttc
     1021 cgctgcctat cgttccggtt cactgatgct tgatgagcgt accggactga ctcacgacta
     1081 cacccgcaaa agcggtgtgg ctgaggctgt gattctgacc cccgccaccg cccctgcctg
     1141 gtgtacgaac cgtgccgaac tctggaatgc cgttgagaag gccgaacgcc ggaagaactc
     1201 acaacttgcg cgggaaattg aactcgccat cccccacgaa ctgccgcagg atgccgcacg
     1261 ggaaaccgtt ctcgcctttg tcagagaaaa ctttgtcagt cagggcatga ttgctgatgt
     1321 ggcgttccat cacatggata aaacaaatcc ccatgcgcac atcatgctga ccacgagggc
     1381 tatcggacct gctgggttca gcggaaaggt cagggactgg aacgaccgga cacatgccga
     1441 gacgtggcgg gcatcatggg ctgaccatgc aaacagggca ctggcgaacg ccggttatca
     1501 ggaagagata gaccatcgtt catacgagcg acaggggctt gagaaaacgc ctggtattca
     1561 cctcggaaag tctgcctgtg cgatggaaac acgcggtatc gaaacagaac gcggcgaaca
     1621 gaatcgtctg attaacaggc ttaacctgga aatacagatt tcccgcaccg aactcaggaa
     1681 cggagggctg acagagcctg cccgccgtgt ggctgacctt ctgaatatcg ggcttcctga
     1741 caacgcaact tcgtacacac tacgtgacat tatcgaagcg ttgccgaagg acagtaactc
     1801 ggcatggaaa ctcaccagta atgccatgtc gatgatggca gatatgcagg cgacgcgcag
     1861 acgctgggaa gagctgaaca ccgaacgaaa ggaagcgatg agccatgctg ccagcctgaa
     1921 aaaatcatcg ccgttttcgt ccggtttttc ccgtattccc ctgatgcaat gggcggcacc
     1981 ggaataccgt agtgagcagg gaaaaatcca gtctctgagc cagcaaatgg aaaaattacg
     2041 acagcattac cggaaggtgg agaaacagga cattcccgcg agtcagatgg catttgaaaa
     2101 acagtggcag cagtgggggg aatccggcct tgcagctctc agagcccagt tgcttaaacg
     2161 tgaagaggaa ttgcgccgga aggaacagga agatactgaa cgtagtcgcg cggagcagct
     2221 caggaaaaat gacaatgccg ttatggattc aggggtgctg agagcagtcg ttacggggta
     2281 tggcgaggct cccatgcctg gtaacgggga aatgacctgt taccttctgc ttcacaaccg
     2341 gaacggagaa tatacgctct gggggaacga gctggagaaa taccgcacca gaatctttga
     2401 gagtgttgat ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg aaatcggcca
     2461 acgtccgcca ctgcaacgtg tttattcctc tgcaaccttt gagcaactgc tgacgcaggt
     2521 ttgccagaca tggccgcaac atacccgcaa tctccgccag ccgaagacat ggccggagtc
     2581 tttctgtctt ggagaagacc gccagccagc catgccatca ccagcggcaa ggaaggtgga
     2641 ttttatgcag ggacgtctgc caccgacgct gatgccggtc atgagttctg tcgaccggga
     2701 aacccgccag cttcagcttt tgctggtcat gggcgtggat gactctctgg gcggtgttat
     2761 ccggcttaat ggtacgctgt atcccgcgtt tgccgttccc tcagccgata acaggcatct
     2821 ggtcatcagt gcgctgaccg ataaggggtt acggtatgca gggtatggtg ttgccgtaaa
     2881 tcacgatgca gacagccaca tatcacctgc accagaactg atgcagtttc atctgaaaac
     2941 ccgtgaggca ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg atcatctgtt
     3001 ccgctcgctg ggttttaatc gtacctggga ggaatggcag cgcgaggaag atgcacgaac
     3061 acacaccacc gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa caatattttg
     3121 ttttgtttaa catcgtgtta tgttcgtcga tgttcttcga tgttcatcga cagggatgaa
     3181 tgcaagaagc acactcacat aacgcaagca tagttttgtt tttccgcccc caaaaattgg
     3241 ggggtctttt ttcgctgcat cagagcgttt ttgacgcaat ttttcacttc aatagtacct
     3301 tcgcactact aacacaaaaa aaacgcagca gggagcgttt taggtgcctt aaaatcgatt
     3361 ttagatcgtc atcttgatct gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt
     3421 gcgccccttt ttgatcgtct caacagttac aaccagatcg cttttttcgt tcagttcggc
     3481 gatggctggc ttgattatca tcttattcaa gtctttgaat tgttgatatt tatcttcaac
     3541 ggcaagcatg gttcggaaat catcgatagc tatcgttctg tcaccggtgc tgcggaactg
     3601 ctggagcagc tcgtaaatgc ggatgctgta ggtgctggaa aggctggaga cgttcttcac
     3661 gactacccgc gtaaactgcc ccttgagctg tgtcagatac ggcatgatgg catcggagaa
     3721 ggtgatatct actttcgcct cacctttgaa atactgcgcc cggtactgca cccagcggaa
     3781 ctcttctcgt ttttcctcgt tcttgatgat tactgacctg tcccagaggc gatctatcgc
     3841 ttccttgagc ctagcttcag cgtgctgccg tcccatgtca ggaaaggcat catgaaactc
     3901 tgcagccgtg atggtcatgg ttttttgttc atccggcgaa ggggaatctt ctccggattt
     3961 caggcggctg atacagacca gcaggaagcg ctgttcctgc aaagtgagct tgtagcttgc
     4021 ctcgataagg gcgtttgatt tgtaagctat cagatctgac atttcgcttg ct
//
