LOCUS       NZ_CP148281             4073 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain Z1323PEC0011 plasmid pZ1323PEC0011-5,
            complete sequence.
ACCESSION   NZ_CP148281
VERSION     NZ_CP148281.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40441618
            Assembly: GCF_037528475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete genome sequence of Escherichia coli isolated from humans,
            companion animals, industrial animals and wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2024) Division of biotechnology, Jeonbuk National
            University, kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037528475.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 20:10:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,881
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,455
            CDSs (with protein)               :: 4,455
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 308
            CDSs (without protein)            :: 308
            Pseudo Genes (ambiguous residues) :: 0 of 308
            Pseudo Genes (frameshifted)       :: 107 of 308
            Pseudo Genes (incomplete)         :: 218 of 308
            Pseudo Genes (internal stop)      :: 46 of 308
            Pseudo Genes (multiple problems)  :: 55 of 308
            Pseudo Genes (short protein)      :: 4 of 308
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Z1323PEC0011"
                     /host="Dog"
                     /db_xref="taxon:562"
                     /plasmid="pZ1323PEC0011-5"
                     /geo_loc_name="South Korea"
                     /collection_date="2023-02-16"
     gene            complement(332..526)
                     /locus_tag="WHU34_RS24390"
                     /old_locus_tag="WHU34_24390"
     CDS             complement(332..526)
                     /locus_tag="WHU34_RS24390"
                     /old_locus_tag="WHU34_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1299..1892)
                     /locus_tag="WHU34_RS24395"
                     /old_locus_tag="WHU34_24395"
     CDS             complement(1299..1892)
                     /locus_tag="WHU34_RS24395"
                     /old_locus_tag="WHU34_24395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012537770.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGGGIASMKCLLALSVLI
                     DFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPNDDR
                     WAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTGVQP
                     NQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
                     /name="hypothetical protein"
     gene            complement(2486..3415)
                     /locus_tag="WHU34_RS24400"
                     /old_locus_tag="WHU34_24400"
     CDS             complement(2486..3415)
                     /locus_tag="WHU34_RS24400"
                     /old_locus_tag="WHU34_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_338913118.1"
                     /translation="MIEMDMPNTIHYPHVIPFISQGKINAIKSTFGNNLSDRECYGIYI
                     WSQKASSAIYPLLQQLEVTLRNSIDKEATKLIGQKWWDNVYTDTSKSKHGDFIHNINKA
                     IRRYENEFKKKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEASRALWPR
                     LTYRVLKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQSAVETIR
                     EKINKIEGLIKTINPQVHLSLTKWGSFYHAKRICSQKEAELHLGKGIINSTTDEMNTIL
                     DQLYALTADGKLTGVVKRNTNNIAFHKF"
                     /name="Abi family protein"
     gene            complement(3626..3739)
                     /locus_tag="WHU34_RS24405"
                     /old_locus_tag="WHU34_24405"
     CDS             complement(3626..3739)
                     /locus_tag="WHU34_RS24405"
                     /old_locus_tag="WHU34_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531573.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="replication initiation protein"
     Typing          165..251
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          563..661
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          786..1041
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.411"
                     /coverage="89.0"
                     /evalue="2.67e-103"
     Typing          complement(3668..4073)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.833"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ataactgtgt taccgttttt gtgtgagtca gtaccgctcg ccgcagccga acgaccgagc
       61 gtagcgagtc agtgagcgag gaagcggaaa agcgcccgga cgtgcatttt ctccttacgc
      121 atctgtgcgg catttcacac ccggcatggg gcacttttca tacaatccgc actgatgccg
      181 catggttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
      241 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgccga aaacgctcgc
      301 ggacaagccg tgagcgcctc cgtcagcttc gttatgttcc tgtttcactt tcggtctcaa
      361 agcgaacctg gatgctgttc tggagttctt ccgcgagttc gtgcagtcgt tcgcacatgg
      421 ctgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
      481 tgataaatcg cgccatattc agcgcagttt gttgttgttt gttcaccttc cgttgttctc
      541 cgtcagttcc gggtgtcggg gggaccctga gcagctggta attgtaagac gttgcgggcg
      601 acggtattac aattgcacat cctgtcctgt ttttttagga cgtatcatgg cacagcatat
      661 caactgataa ataatccctg tgcagtgcag ccgctgttgc ataaaatttc gctctgcgtc
      721 gagtgtttct taatcaataa ttccgctgat actccggaaa aacgcgagtc tgttctcatt
      781 cctgaaatgc ccacacccca cgcaaaaaca agtttttgct gatttttctt tataaataga
      841 gagttatgaa aaattagttt ctcttgctct ctttgcgata tttaaaaaag cggtgccggc
      901 gcagctacaa caccgcgccg acaccgcttt gtaggggtgg tactgactat ttttataaaa
      961 aacatcattt tatatcaggg gtgctgctag cggcgcggtg tgttttttta caggacgccc
     1021 ctgggggcgc tgccaggggt gtctgttcag atatgtagca atgagtcagg gattcacgaa
     1081 ctgaaaatgt ccgaaaatgt gtctcatggg caccgtatgc gcccaaaccg ttcgtcattc
     1141 attttcatgc atagctatgc agtgggctga aaacgatcct gacgcatttt ttcggttttc
     1201 actgggtaaa acatctcttt ttgcggtatc tgcgtcagaa tcgcgttcag tgcgttttag
     1261 cggtgcgcgt aatgcgacgt tatggtaaat gcatttcctc ataggccgat tatttcatag
     1321 atgtttgact ctctatcttc ttttggttgc agatgtatca ggcgactaca gtacagtaca
     1381 tctaagccga acctaatctg gtttggctgg acacctgtat agcttctcag tgtttcatgt
     1441 gtcaccttaa cagtattatt tgactgataa cgaaggctta taattgttaa atatagtttt
     1501 aatgccacaa atggggccat ccccctgttc gatatggtgt ttagtttttt cctgatagta
     1561 tctactggta cttttgccca cctatcatca tttggctgta ctgttaattc ataggagtta
     1621 acaaacatct ctctatctat ctttataaga ccatctttct caagtttggc tactcctttc
     1681 actatcatgg ggcggcttag tccagtcagt ctttccattc cggtaaatga tactgaggct
     1741 gttcttgaat ggaaatcaat caagacggat agagctaaca agcatttcat tgatgcaatg
     1801 ccgcccccta tctcatttga acgaacattg tttacaagcc atgagcttcg ggcccaccag
     1861 tttggaagcc tgccaaattt tctaccgctc atttttaccc cattttcggt caaggagaga
     1921 tataaatagc tttattatca tatacatgaa aaccattgca cctaaagata caacagaaac
     1981 caaaacctcc ggctttgcgg aagttagcgt taccagcaaa ttgttgaggg tttccaattg
     2041 gtcctccagt cgcatactaa gttgttgtgt tttaacttta tatacgctta ttagaataaa
     2101 tctagataaa gcaatatttg gtggtttctt gttttcccat ataaaacatg atgttatctt
     2161 gtttttatcg ttttttaact ggtaaatgta aatttgataa tgctggttta atcggcgttt
     2221 ttagttattt ttgttgtgtg ttttttgatc tattggtaaa tggtggatgg taagctttgg
     2281 cgtgatcatg ggaaatagtt tgctcttttt cctgatgatg atgtatcatg tatggcagtg
     2341 aagccttgag ctttcgcttt aatgcattgt gaaattttta tagattcaca atgacattaa
     2401 ggccacgctc caccgaaagg tgataatggc aagagatttg gatgaggctc gacaggaaac
     2461 tgtcgggcct tttctatttt gtgatctaaa acttgtgaaa tgcgatattg ttggtatttc
     2521 gttttacaac acccgttagc tttccgtcag cagttagtgc gtatagttga tctagaattg
     2581 tgttcatctc atctgttgtg ctattgatta ttcctttgcc tagatgcaat tcggcctctt
     2641 tttgagaaca tattcttttg gcgtgataaa aggaacccca cttcgttaat gataggtgta
     2701 cctgtgggtt tatcgttttt atcagtcctt ctattttgtt tattttttct cttattgttt
     2761 ctactgcaga ttgcaaattt ttagagtgta tttttatcca tatgcaatca ttatgcgata
     2821 acctatttct gtagtttctg atctcattca gctcgtttaa gaaatcaatt ctggctgtgc
     2881 cttcatcttt gcttctatct aatcccttca gtactctgta ggtgagcctg ggccataagg
     2941 ctcttgatgc ttcagatctt gattgtgtat gaaaagcatc gctcaaaacc gcttgccatg
     3001 tgtaaaaatc tgtgtgtgca atgatgtcat catgaggtgc aattattttg gtatttgcca
     3061 ttgatgggtt ttttttcttg aattcattct catatcttct tattgctttg tttatattat
     3121 gtatgaagtc accgtgtttg ctttttgatg tgtcggtgta tacattatcc caccattttt
     3181 ggcctattag ttttgtcgct tctttatcaa tcgagtttct caaagtaact tcgagttgtt
     3241 gcagtaatgg atatatggca gatgaggctt tctggctcca tatatatatc ccgtagcact
     3301 ccctatcaga caggttatta ccaaacgtgc tttttatagc atttattttt ccttgggata
     3361 taaatggtat tacatgaggg taatgaattg tatttggcat atccatctct atcatacact
     3421 tagtggtgat tggatgttgc catacacgta tggatagttg tagagtgtta aacgaaaaaa
     3481 ccgccttggc gggcggtttt tcgaaggttc aaagagttgg cgctctgtga accaaggtaa
     3541 ctggcttgga ggagcgcagt caccaaaatc tgttctttca gtttagcctt aacaggtgca
     3601 taacttcaag acaaactcct ctaaatcagt taccagtggc tgctgccagt ggcgttaagg
     3661 cgtgccttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3781 accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cggtttttgt
     3961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag cggcgcggcc ttttttcctg
     4021 gctttgcctt gttgctcaca tgttctttcc ggtcttatcc cctgattctg tgg
//
