LOCUS       NZ_CP148078             3314 bp    DNA     circular CON 05-APR-2024
DEFINITION  Staphylococcus aureus strain BSN155 plasmid pUSA01, complete
            sequence.
ACCESSION   NZ_CP148078
VERSION     NZ_CP148078.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637734
            Assembly: GCF_037482255.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN155 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN155 contigs
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP043386, CP053637, CP014442
            Genome Coverage                   :: 95.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037482255.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:23:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,969
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 44 of 83
            Pseudo Genes (incomplete)         :: 42 of 83
            Pseudo Genes (internal stop)      :: 16 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP148078.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN155"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pUSA01"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-11-10"
                     /collected_by="Andrew D. Berti"
     gene            1..996
                     /locus_tag="UXR73_RS14835"
                     /old_locus_tag="UXR73_14835"
     CDS             1..996
                     /locus_tag="UXR73_RS14835"
                     /old_locus_tag="UXR73_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1768..1956
                     /locus_tag="UXR73_RS14840"
                     /old_locus_tag="UXR73_14840"
     CDS             1768..1956
                     /locus_tag="UXR73_RS14840"
                     /old_locus_tag="UXR73_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2282..2863)
                     /locus_tag="UXR73_RS14845"
                     /old_locus_tag="UXR73_14845"
     CDS             complement(2282..2863)
                     /locus_tag="UXR73_RS14845"
                     /old_locus_tag="UXR73_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1067..1231
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          2..996
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatat
     1141 cactttaaaa taccttaaaa cccctggaat ttctggcttt gccagaccta tcatttttga
     1201 atgatagcaa attctcctta tgctcttacg gagtttttag agaaaaatta aaaattagca
     1261 taaaatcctt taaaacttcc acatgttttt ctaaattcct gcagggcgtt ttttatcaaa
     1321 aatcgagaat ttttaatttt tctctaaaaa ctccgtaaga gcataaggag aatttgctat
     1381 catttttgaa tgatagcaaa ttctccttat gctcttacgg agtttttaga gaaaaattaa
     1441 aaattctcga tttttgataa aaaacgccct gcaggaattt agaaaaacat gtggaagttt
     1501 taaaggattt tatgctaatt tttaatttgc atgtaaatgg gcagtgttaa aaaattagtc
     1561 actggttttg ctgaaatttt tgtttgtgaa attaccatat atttatttgg ctcatatttg
     1621 cgttttaaga gcttgtatat atttttaagt taaaaaccta tatgataccc cctaaacctt
     1681 taaaataccc ccttaaaact caaaataaag gtatttataa tttgagtaag tcttgtgata
     1741 aagtttttta aaaaaggagt gtttattttg aataaaaaca cagtgttaga tgaaggttac
     1801 gttatagcaa ctattttaaa tgttttcttt ttacttggat taatttttat atctcatttg
     1861 gaaaatttat atatattaat tccgtatgtt attttgatgg gaataaatgc tatttatttg
     1921 gttgttaagg ttatgaattt caaaaagaat aattagaata tttgagggga ttttgaaacg
     1981 agtttcttct tgttttcaca ctgttttttt attcctattg gtgttgttgc ttactttttg
     2041 tttttctata aagatgatgc ttttgacgag atggaagaaa aatatgatta tcatgaagat
     2101 aataaaaaat agacgacgca tttatgccga gaaaatttat tgatgttgag aagaaccctt
     2161 aactaaactt gcagacgaat gtcggcatag cgtgagctat taagccgacc attcgacaag
     2221 ttttgggatt gttaagggtt ccgaggctca acgtcaataa agcaattgga ataaatagta
     2281 tctaaagtaa cccgtattta agattacatt gtacatcctt cgaaaccata gtagctgcga
     2341 ttccagctag gtttcctttt atcccaattt tagctaattt ttttgctacg ccctttaagt
     2401 ttttggcttt aacatcttca aaaactgctg tgattatgtt tccagttaaa agttctccat
     2461 atgagtttaa aacttcagaa tagaaacagt caccgctatt tttgtagttc ccaccaactg
     2521 ctctaggtga tattgaacta gttttctttt tattctctat gtcgtttttt agtttttgga
     2581 atccttcagg aacatatcca tatcgagatt tggcattttc tagattaaca tctttagctg
     2641 ttccgtcttt atctaaaata ataattttgc catatatgta ttttagttct ttttcaattt
     2701 ctttttcgtt taaagtttca acgtttttat ctgcaatgtt atttttgttt tgggcttgag
     2761 cttctttgaa attaaaatcc aagaccattg aaagtatggt taaaatactt aaagatacta
     2821 ctaaaatctt tcttagtgat atactgtttt gaattgcatt cattgtaaat gcccccttgt
     2881 tttgttattt aaatgaattc aatataatat taacatataa aaacaaagga gtttaatatg
     2941 aataagtttt tttacttttt gattatttta ttatctgtat ttttaatttt taattttgtt
     3001 aattcgattt tatttgataa tccttttgac tttttagaaa gttttattta ttcattggtt
     3061 ttcacattaa tttatttagg tttagagggt cataaaaaga agaaggaata ggttgttttt
     3121 tgaaacgagt gtgaacgagt ttcttcttgt cttgatacta tatagaaata actcgatttt
     3181 atatatatag ctgtaactgt tgatattaca gtgtttaaac gtgtttttgt gcgtgaaagg
     3241 aaaatttgac aataaaaaac cccagttata ttattaaggt gtcgaatctt aaataatact
     3301 gggggtcttt ttat
//
