LOCUS       NZ_CP147837             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 130120D plasmid p3_130120D, complete
            sequence.
ACCESSION   NZ_CP147837
VERSION     NZ_CP147837.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40411204
            Assembly: GCF_037329525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of genetically modified Klebsiella pneumoniae
            strain 130120D
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2024) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037329525.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:09:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,622
            CDSs (total)                      :: 5,502
            Genes (coding)                    :: 5,351
            CDSs (with protein)               :: 5,351
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 61 of 151
            Pseudo Genes (incomplete)         :: 96 of 151
            Pseudo Genes (internal stop)      :: 19 of 151
            Pseudo Genes (multiple problems)  :: 25 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147837.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130120D"
                     /isolation_source="experimental"
                     /db_xref="taxon:573"
                     /plasmid="p3_130120D"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2021"
     gene            complement(join(5492..5596,1..54))
                     /locus_tag="WEO27_RS28060"
                     /old_locus_tag="WEO27_28060"
     CDS             complement(join(5492..5596,1..54))
                     /locus_tag="WEO27_RS28060"
                     /old_locus_tag="WEO27_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            694..870
                     /locus_tag="WEO27_RS28065"
                     /old_locus_tag="WEO27_28065"
     CDS             694..870
                     /locus_tag="WEO27_RS28065"
                     /old_locus_tag="WEO27_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            860..1204
                     /locus_tag="WEO27_RS28070"
                     /old_locus_tag="WEO27_28070"
     CDS             860..1204
                     /locus_tag="WEO27_RS28070"
                     /old_locus_tag="WEO27_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1413..>1847
                     /locus_tag="WEO27_RS28075"
                     /old_locus_tag="WEO27_28075"
                     /pseudo=""
     CDS             1413..>1847
                     /locus_tag="WEO27_RS28075"
                     /old_locus_tag="WEO27_28075"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1882..2391
                     /locus_tag="WEO27_RS28080"
                     /old_locus_tag="WEO27_28080"
     CDS             1882..2391
                     /locus_tag="WEO27_RS28080"
                     /old_locus_tag="WEO27_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2398..2610
                     /locus_tag="WEO27_RS28085"
                     /old_locus_tag="WEO27_28085"
     CDS             2398..2610
                     /locus_tag="WEO27_RS28085"
                     /old_locus_tag="WEO27_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3277..3501
                     /locus_tag="WEO27_RS28090"
                     /old_locus_tag="WEO27_28090"
     CDS             3277..3501
                     /locus_tag="WEO27_RS28090"
                     /old_locus_tag="WEO27_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3485..3751
                     /locus_tag="WEO27_RS28095"
                     /old_locus_tag="WEO27_28095"
     CDS             3485..3751
                     /locus_tag="WEO27_RS28095"
                     /old_locus_tag="WEO27_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3821..4171)
                     /locus_tag="WEO27_RS28100"
                     /old_locus_tag="WEO27_28100"
     CDS             complement(3821..4171)
                     /locus_tag="WEO27_RS28100"
                     /old_locus_tag="WEO27_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4237..4782)
                     /locus_tag="WEO27_RS28105"
                     /old_locus_tag="WEO27_28105"
     CDS             complement(4237..4782)
                     /locus_tag="WEO27_RS28105"
                     /old_locus_tag="WEO27_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4808..5134)
                     /locus_tag="WEO27_RS28110"
                     /old_locus_tag="WEO27_28110"
     CDS             complement(4808..5134)
                     /locus_tag="WEO27_RS28110"
                     /old_locus_tag="WEO27_28110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          529..608
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4..334)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.82"
                     /coverage="81.0"
                     /evalue="2.28e-115"
     Typing          1195..2726
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatagtta ccggaaaggg cgcagcagtc gggctgaacg gggggttcgt gcatacagcc
       61 cagcttggag cgaacgacct aaaccgagcc gagataccag cagcgtgagc tatgagaaag
      121 cgccacgctt cccgaaaggg aaaaaggcgg aacaggtatc cggtaaacgg cagggtcgga
      181 acaggagagc gcacgaggga gccgccggag agaaatcacc ggcatcttta agtcctgtcg
      241 ggtttcgtcc tcctctgact tgagcgtcca tttctgtgat gctcgtcagg ggaggcggag
      301 cctatggaaa aacggcaacg ccgcatcctt ttctccggcc ctttctgatt tgagcgcctt
      361 tgctttatcc cgttatccgt gagtcggtca cgcccgccgc agccgaacgg caggagcgca
      421 gctaccgagt gagcgaggag gcgtcatttc atctgtccgt gcatggtatt gcggacgctc
      481 ctgagccaca tatcacacct gccacatgag gcactttcat gaatcgtgct ctcatgccaa
      541 catagtaagc cagtatacac agcgtaaacg ctgtgactgg ttcagggctg cgccccgaaa
      601 cccgctaaca ccactgacgc gctggcgctt gtccaacgcc ggagcgaccc ggaaaacttt
      661 tccgtgccgt ccggcgtttg cgaaggaggg ataatgcaga agccgaagaa gaagccgctc
      721 gtcgtaaaaa aaagatcctg gcatccggca gtgatggcaa atctgatgcg tcaggcacag
      781 gaggaagatt accgccggga gcagatggca gagcttgagc gttcagcccg gtcacatttt
      841 gcggatatcg gaggagagga tggcggttaa gcgtacccgt tttttaggta tccgggtgac
      901 cgatggggag taccagcagc tgctggagcg ctgcaatgga aggcaactgg cggtgtggat
      961 gcgggaaacc tgtctcgata cgcgcccggc gcggtcgttg cggctgccgt ccatcgatcc
     1021 ggttttgctg cgccagcttg ccgggatggg gaacaacctc aaccagatcg cccggaaaat
     1081 taacggcggc cagtggtcag gcgctgaccg ggttcaggtg gtggccgcgc tgatggccat
     1141 tgatgccgaa ctcgagcggc tgcggcatgc cgtgctggaa aagggggcag acgatgatcg
     1201 ttaagtttca tccgcgaggg cgaggcggcg gtgccgggcc ggtggattat ctgctgggga
     1261 aagaccgcca gcgcgacggg gccatcgtcc tgcagggaaa gccggaggag gtccgggagc
     1321 ttatcgacgc ctcgccctac gccaaaaagt acacctccgg cgtcctgtct tttgctgaac
     1381 aggatttacc gcccggccag cgtgaaaagc tgatggcgag cttcgagcgg gttctgatgc
     1441 ccggactcga taaagaccag tacagcgtgc tgtgggttga acaccgggac aaggggcggc
     1501 tggaactgaa ttttctggta ccgaacacgg aactgctgac cggcaggcgt ctccagccgt
     1561 actacgaccg ggcagaccgt ccgcgcatcg atgcgtggca gaccgtggtg aacgggcgac
     1621 tggggctgca cgacccgaac gcaccggaga accggcgcgc gctggtgacg ccatcttcgt
     1681 tacccaaaac gaagcaggaa gccgcagagg ctattacgcg gggcttactg gccctcgcct
     1741 cgtcagggga gcttaaaacg cgtcaggacg tcactgaggc gctggaaagc gcaggttttg
     1801 aggtcgtgcg caccacaaag agcagcatca gcattgccga cccggacgga gggcgaaaca
     1861 tccgacttaa gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag
     1921 aaatcgaaag cgcagcagca gagtaccggc gaaatgctga aagccgcatt cagcgagcac
     1981 gagaagtctg tcagagcgga actgagcgaa agcgggaaga gaatcagcgc cgccatccgc
     2041 gcccacgagc agggtatgag cgcggccatg cagtcgaacc gcctgagcgt gatgcgtatg
     2101 gtcgggcgga catggctgac catcacgatg gtgtcaggac tgctgttcgc cagcctgagc
     2161 ggggtgttgt ggtatcaggg gagcttgata gcgtcaaatc tggccgaaat cgaccggcag
     2221 aacgcagcgc tgtcgaagct gaacgcgaag acctggggcg tgacgtacct ggaagacagc
     2281 aacggacgtt ttctggtgct gccgaagggg acggccgtag acaggacgca gagctggacg
     2341 gtcgggaacg ggcggtcgaa gcagaacgcg ctgagactgg tgaaggagta agtgccgatg
     2401 accgagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa
     2461 aggctggacg agtgggagag cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa
     2521 cgggagaacg cggcgctgag cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg
     2581 agcgggcaac tgcagcgttt gagccggtag tgcagcgtca tgagatggcc gtggccgcag
     2641 aacgggcgca cgagcaacgg cagcacgaaa aggaactgac ggaagcgcgt tcccggcagc
     2701 gaagctatga cggtccgtcg ctgggctgaa atgcaggaaa acagggttct catcgggcat
     2761 ttacgctgaa accggatgtc attcacctgg ctgcatggct atgcagccag gtaaaattta
     2821 tctgtcgcga acggggcggt ttatcgggtg gtttgttgcc ggttttacct gactgccacc
     2881 ccgttcgcgg cgaacccgtc cggggcggtg cgggcaacgg gtgttatgtt taatcagcgt
     2941 gaacggcaga tacaaaaaag ccccggaggg cttttaacta ctgacttatt tttttgaaga
     3001 tttgctgggt ttcaatttgt cagtatttcc cggcgttttt ttattgtcac gctggcgttt
     3061 atcaggttcc cctgttgaat cggcaattcg tttcggaccc ggcttaagac ccataatcgt
     3121 tatcctttca atagttaaga ggaaagccct caagctagat atattgaccg cgttacggat
     3181 tatcaagagg ggctaacaag attatttttt gttcttttag gttgattgtg gtactgttga
     3241 tatacatgta tagcaaatgg tgttcgggag gtttatatgc ttgctatccg gttatctgat
     3301 gagattgagt cccgtctgga ctcgctggcg aagcaaaccg gcagaacaaa gacgttttat
     3361 gcgcgggaag caatactggc gcatctggaa gacctggagg attattatct ttcagcagaa
     3421 actgctgcac gcgttcgccg tggtgatgaa gcagtgcatt cgtctgaaga cgtgaggaag
     3481 tcacttggtc tggacgatta actattccga tcgggcgctc aaatcgttac gcaagatgga
     3541 caaacagaac gcacgacgga ttgtggattt tatgagttta cgcattgcag ttgctgccga
     3601 tcctcgccag tcagggaagc cgctcaaagg tgagctgggc gagttctggc gctatcgggt
     3661 gggagattat cgcgttctgt gtgagatccg agatgacgag cttgttatcc ttgccgccac
     3721 gattggacat cgccgcgaag tttacgactg aagcccgcta tgcgggcttt tttgatgccg
     3781 acaacaggta ttatggtaaa tactcgacta actacgtgtt ctactgtttg tcggttttag
     3841 ttaaccagca ggttgtctta acgataatgt agccgttatt tttaactaat aaggtcatat
     3901 tgttaacgtc aggcataata aaagacatta tggcctcaga gttatcatca gaagcagctc
     3961 tctgcattac tacttcgatt cccttttctt tgaatctata agtatccagt tgcctattaa
     4021 acttagaaag acctacaacc atacttagtt tgttatcttt aacagtaaga gatgcttcct
     4081 gagtaccatt acatcggtag taagcggtgt aaccgtcagc aaaagatcga ccggggaata
     4141 taaatgctag gaacgcaatt aaccaataca tagttgagaa tcttggtatt acacatttca
     4201 ctatcctctc tttcattttt cgttcaccat actttgttat ctgattttta atttttctat
     4261 tttataaacc aagtctctac caaaaaggcg aaaattatcc tcaaataata ataattgttt
     4321 aagaacatcc atgaaagcag ctgcttcacc ctcagttatg ggagctactc tatataacat
     4381 ataacctgag ttaagggttt cttttatact ttcttcaatg ctcattataa atattagtgg
     4441 attatcattt ttcttccccc atgggtttct aattaatcct tcgatgtact caggtccttt
     4501 gcgtcctatt gcctgcgcaa tattttttat ttcatctgcg tgagggcttt gttgtacagc
     4561 tttgtttatg cctgcttgag tgattttcag agactctaca ccgtgtatgt tgtatgagat
     4621 aaaatgtaat aaattaagat gcaatgcaat taatccattt ttgtttacat ctgagtattt
     4681 ggataaaaaa cgtaatgaag tgttaactct cccttgaagc ctaacaattg ggagtgctgc
     4741 cttgtatatt tccttctccg ttaaataccg tttaaaaaac ataattctcc gcctccgtat
     4801 agcgtgccta ttttatttcg tcgtaaaact tagctctgaa atattccttt agaaccatca
     4861 tgtcattaat aattgaatct atctcatcca ttttttcttg gtattctttt aatggtgaag
     4921 ggagcggtaa ctcaatattt gaaaagtgat tggcgagata gggatcaagg tttctcatcc
     4981 attcaggctt ttcgccaatg cgttcttcaa ctgaaagtga acctgataat ctctggttaa
     5041 taagatcact tagactcatt ttggttttat tcgaaagccg ttgtaaggat tgctttaatc
     5101 ctatatctag catgatattt gttctttctc tcattacccc cccttgctat tgcgtttttt
     5161 gttgcgcaca aagctaccat tgcgcattaa gaaacgcaat aaaaaaggag ggagggagtg
     5221 gtttgtacat gatgtgcagt gcgctgaggg catcagtggc gggtgttttt ccgtgggatg
     5281 gatggagctc cggggataac agaactgacg atcatttaaa ggatcttctt gagatccttt
     5341 ttttctgcgc gtaatctctt gcactgtaaa cgaaaaaacc gcctggggag gcggtttgat
     5401 cgaaggttaa gtcagttggg gaactgctta accgggtaac tgggtatacg agaccgcaga
     5461 taccaaaact gttctttcag tgtagccgca gttaggcacc cacttcaaga cctctcaata
     5521 tctctctcgt atatatccag ttaccaatgg ctgctgccag tggcgttttt gcgtgtccgt
     5581 ccgggttgga ctcaag
//
