LOCUS       NZ_CP147836             9763 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 130120D plasmid pKPC84_130120D,
            complete sequence.
ACCESSION   NZ_CP147836
VERSION     NZ_CP147836.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40411204
            Assembly: GCF_037329525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of genetically modified Klebsiella pneumoniae
            strain 130120D
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2024) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037329525.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:09:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,622
            CDSs (total)                      :: 5,502
            Genes (coding)                    :: 5,351
            CDSs (with protein)               :: 5,351
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 61 of 151
            Pseudo Genes (incomplete)         :: 96 of 151
            Pseudo Genes (internal stop)      :: 19 of 151
            Pseudo Genes (multiple problems)  :: 25 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147836.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130120D"
                     /isolation_source="experimental"
                     /db_xref="taxon:573"
                     /plasmid="pKPC84_130120D"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2021"
     gene            complement(join(9649..9763,1..590))
                     /locus_tag="WEO27_RS27980"
                     /old_locus_tag="WEO27_27980"
     CDS             complement(join(9649..9763,1..590))
                     /locus_tag="WEO27_RS27980"
                     /old_locus_tag="WEO27_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            615..815
                     /locus_tag="WEO27_RS27985"
                     /old_locus_tag="WEO27_27985"
     CDS             615..815
                     /locus_tag="WEO27_RS27985"
                     /old_locus_tag="WEO27_27985"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072354025.1"
                     /translation="MISLSPPTICNSARETALPESVLREPQREREAVREVARENLLQER
                     LQQMERDMVRDLQKEKTLGGD"
                     /name="hypothetical protein"
     gene            835..1581
                     /gene="traX"
                     /locus_tag="WEO27_RS27990"
                     /old_locus_tag="WEO27_27990"
     CDS             835..1581
                     /gene="traX"
                     /locus_tag="WEO27_RS27990"
                     /old_locus_tag="WEO27_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="WP_000205770.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLMV
                     LDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAY
                     YLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLM
                     LAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKS
                     LPRFWPGDFFPVFYACHLAVLGVLAL"
                     /name="traX"
     gene            1636..2196
                     /locus_tag="WEO27_RS27995"
                     /old_locus_tag="WEO27_27995"
     CDS             1636..2196
                     /locus_tag="WEO27_RS27995"
                     /old_locus_tag="WEO27_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="WP_000139328.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLAE
                     KAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRDVL
                     LEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEAYAA
                     ARLDKIRRQNRIKAELQAVLDEK"
                     /name="fertility inhibition protein FinO"
     gene            2328..2528
                     /locus_tag="WEO27_RS28000"
                     /old_locus_tag="WEO27_28000"
     CDS             2328..2528
                     /locus_tag="WEO27_RS28000"
                     /old_locus_tag="WEO27_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059022.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAARV
                     APRTDWQQFARQRAERCQRRR"
                     /name="hypothetical protein"
     gene            2914..3513
                     /locus_tag="WEO27_RS28005"
                     /old_locus_tag="WEO27_28005"
     CDS             2914..3513
                     /locus_tag="WEO27_RS28005"
                     /old_locus_tag="WEO27_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="WP_032083981.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNNV
                     ALPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYDGI
                     VRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTFNNY
                     LKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
                     /name="PIN domain-containing protein"
     gene            3575..3907
                     /locus_tag="WEO27_RS28010"
                     /old_locus_tag="WEO27_28010"
     CDS             3575..3907
                     /locus_tag="WEO27_RS28010"
                     /old_locus_tag="WEO27_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152916585.1"
                     /translation="MAFISMTRQKAPYRYASKTRLSIRRTDSGSAGCYVTVAVRGKQEF
                     IIAELDSEIRKIRLKLTDSYEEGVRLSSGTFTLPARFCREILPDDVRSITIILEKSDDE
                     WWYGSY"
                     /name="hypothetical protein"
     gene            4212..4361
                     /locus_tag="WEO27_RS28015"
                     /old_locus_tag="WEO27_28015"
     CDS             4212..4361
                     /locus_tag="WEO27_RS28015"
                     /old_locus_tag="WEO27_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001312851.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAY
                     EARK"
                     /name="Hok/Gef family protein"
     gene            4645..4893
                     /locus_tag="WEO27_RS28020"
                     /old_locus_tag="WEO27_28020"
     CDS             4645..4893
                     /locus_tag="WEO27_RS28020"
                     /old_locus_tag="WEO27_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083837.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKELR
                     VFIPAALKAQLQEMCDAEGVTQAEMIAELIKQKSAFS"
                     /name="replication regulatory protein RepA"
     gene            5138..5212
                     /gene="tap"
                     /locus_tag="WEO27_RS28025"
                     /old_locus_tag="WEO27_28025"
     CDS             5138..5212
                     /gene="tap"
                     /locus_tag="WEO27_RS28025"
                     /old_locus_tag="WEO27_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375168.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]; GO:0031556 - transcriptional attenuation by ribosome
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="WP_001375168.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGWCD"
                     /name="tap"
     gene            5205..6062
                     /gene="repA"
                     /locus_tag="WEO27_RS28030"
                     /old_locus_tag="WEO27_28030"
     CDS             5205..6062
                     /gene="repA"
                     /locus_tag="WEO27_RS28030"
                     /old_locus_tag="WEO27_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_032495102.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESDAGVLSITRATRALTSLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDVSEV
                     AVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDADRERQDIITLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            7001..7654
                     /locus_tag="WEO27_RS28035"
                     /old_locus_tag="WEO27_28035"
     CDS             7001..7654
                     /locus_tag="WEO27_RS28035"
                     /old_locus_tag="WEO27_28035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000616807.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLFF
                     SHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALALPA
                     EPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGALITS
                     LLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
                     /name="CPBP family intramembrane glutamic endopeptidase"
     gene            7747..8004
                     /gene="pemI"
                     /locus_tag="WEO27_RS28040"
                     /old_locus_tag="WEO27_28040"
     CDS             7747..8004
                     /gene="pemI"
                     /locus_tag="WEO27_RS28040"
                     /old_locus_tag="WEO27_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="WP_000557619.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
                     /name="pemI"
     gene            8006..8338
                     /gene="pemK-mt"
                     /locus_tag="WEO27_RS28045"
                     /old_locus_tag="WEO27_28045"
     CDS             8006..8338
                     /gene="pemK-mt"
                     /locus_tag="WEO27_RS28045"
                     /old_locus_tag="WEO27_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="WP_000439434.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPV
                     TSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVLGR
                     LSTILT"
                     /name="pemK-mt"
     gene            8587..>9002
                     /locus_tag="WEO27_RS28050"
                     /old_locus_tag="WEO27_28050"
                     /pseudo=""
     CDS             8587..>9002
                     /locus_tag="WEO27_RS28050"
                     /old_locus_tag="WEO27_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<9071..>9583)
                     /locus_tag="WEO27_RS28055"
                     /old_locus_tag="WEO27_28055"
                     /pseudo=""
     CDS             complement(<9071..>9583)
                     /locus_tag="WEO27_RS28055"
                     /old_locus_tag="WEO27_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     Typing          5308..6062
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtaagccg tcttcatgga tttaaatccc agcgtggcgc cgattatccg tttcagtttg
       61 ccatgatcgc attcaatcac gttgttccgg tacttaatct gtcggtgttc aacgtcagac
      121 gggcaccggc cttcgcgttt gagcagagca agcgcgcgac cataggcggg cgctttatcc
      181 gtgttgatga atcgcgggat ctgccacttc ttcacgttgt tgaggatttt acccagaaac
      241 cggtatgcag ctttgctgtt acgacgggag gagagataaa aatcgacagt gcggccccgg
      301 ctgtcgacgg cccggtacag atacgcccag cggccattga ccttcacgta ggtttcatcc
      361 atgtgccacg ggcaaagatc ggaagggtta cgccagtacc agcgcagccg tttttccatt
      421 tcaggcgcat aacgctgaac ccagcggtaa atcgtggagt gatcgacatt cactccgcgt
      481 tcagccagca tctcctgcag ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg
      541 gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat gtgcagctcc
      601 atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt gcccgggaaa
      661 ctgcgctgcc ggagagtgtg ctgcgtgaac cacaacggga gcgggaagcg gtccgggagg
      721 ttgcccggga aaatctgctg caggagcgtc tgcagcagat ggagcgggat atggtccgtg
      781 acctgcagaa agagaaaacc ctgggcggag actgatacag gaaggcaaac gctgatgaca
      841 acggataaca cgaacacgac acgtaacgat tcactggctg cccggaccga tacctggttg
      901 cagtcgttgc tggtctggtc acccggacag cgggatatca tcaaaacggt ggcactggtg
      961 ctgatggtac tggaccacat caatctgata ttccagctga agcaggaatg gatgtttctg
     1021 gccgggcgtg gagcctttcc gctgtttgcc ctggtgtggg ggctgaatct gtcccgtcat
     1081 gcgcatatcc ggcaaccggc cattaaccgg ctgtggggat gggggattat tgcccagttc
     1141 gcatattatc tggcaggctt tccctggtat gaggggaata tcctgtttgc ctttgcagtg
     1201 gcggcacagg tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc agcggccatt
     1261 cttctgatgg cgctgtgggg gcctttgtcc ggcaccagtt acggcattgc cgggctgctg
     1321 atgctggcag tcagccaccg gctgtaccgg gcggaagaca gagcggaacg tctggcgctg
     1381 gtcgcctgcc tgctggctgt tattccggcg cttaaccttg ccaccagtga tgcagcggcg
     1441 gtggccggtc ttgtgatgac ggtgctgacc gttggtctgg tgtcgtgtgc aggaaaatca
     1501 ttaccccgtt tctggcccgg ggattttttc ccggtattct acgcctgtca tctggctgtg
     1561 ctgggcgttc tggcgctgtg acgggtgtgg tatctttgac cgcaagagga tgattcgtca
     1621 gaggcagaac acagcatgac agagcagaag cgaccggtac tgacactgaa gcggaaaacg
     1681 gaaggggaga cgcctgtccg caggcggaaa accatcatca atgtcaccac gccaccaaag
     1741 tggaaggtga aaaagcagaa gctggccgag aaagcagccc gggaagcaga gctggcggca
     1801 aaaaaagcgc aggccagaca ggcactgtcc atttatctga atctgcccac gctggatgag
     1861 gccgtgaata ccctgaaacc ctggtggccg ggattgtttg acggtgacac gccccggctt
     1921 ctggcctgcg gtatccggga cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc
     1981 tcgcataaaa aactgcgcag ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt
     2041 gccatgaaag ccggtgcctg ccggtatgac acggaagggt atgtgacgga gcatatttct
     2101 caggaggagg aagcgtatgc ggcagcgcgt ctggataaaa tccgccgcca gaaccggata
     2161 aaggcagaac ttcaggccgt gcttgatgag aaataaaaaa aagcctccat cagagaggag
     2221 gcagggaaat aaggcttaaa aggaaataac ttccgtaccc gaaaggaagc caattcagta
     2281 gattatttaa caccataata ctgacgggtt aaagaggagt gataaagatg aatggattca
     2341 gaaacagttc acggaacggt caggtctggc gctggcagcg tgccggaagc cgtgccgtca
     2401 gcctggaggt caggggattc cggaaggagg ccgctgaagc gtggctcctg gctgcccgtg
     2461 ttgctccccg tacagactgg cagcagtttg cacggcagag agccgaacgc tgtcagcgac
     2521 ggagataatg aaaaaaaacg gcaaaaaaaa gacttcccct gagcaaagga ggccaaaaat
     2581 cattcatagc gtattactga cattgaatga atgctctgta catcaaccct gtgaagctac
     2641 caccggttaa tgccgggagg ttgcgggaag aaagataata cacagtcatt acgtggttaa
     2701 tatctgtcag aatgtttttt gtgaccataa actgtcagat ggataaaaaa gaccgtcatg
     2761 tgacggtcag tgttgtcctg tcagggacaa atgcattcag taaggagagc aaaataataa
     2821 catcacgttg cccggctgtt aatatcaggc gatgttaagt gttatttatg actcttttat
     2881 ctggttgtga agtctgttaa tcaggaagag aaaatggcga ccggaaaatc agagaagcaa
     2941 caggtgattg cctttatcga ttacgagaac tgcagtaacc tgacagaaat ttccttaaat
     3001 atatatacgg agttaattat attcgcgggt gcgaaacaga ataatgtggc actgccagta
     3061 aacgctttcc ctgaagcagt aaaaatcaca ctccggcatg tcagtgaagt ctcccggaac
     3121 aatgttgatt ttcatctggt gctggagctg gggcagtgtg tatgccacta cgggccggag
     3181 aaggaatacc atattgtttc cgctgacaag ggatatgacg gtattgtccg gacactgcag
     3241 gcgcgcggga ttcgctgccg tcgcgtctcc ccggataagg cgtcatcagt aaagatggct
     3301 gcacccgaca tggatatcgt tatccggtgg gcaaacaaaa ttcaacaggc agcgcgggag
     3361 gttcgtaata tgccggtaac agaaaagaca tttaataatt atttaaaatc acagatgcgc
     3421 ggtgaatggc gtgatgtcct gaccagaggt atccgggatg aactggtccg tcgcggggta
     3481 atgaaaattc aggggaataa aatcatctgg taagactgcc agaataaaat gatatttatt
     3541 ttatttataa aaacagaaag gtcgaaaatc aataatggca tttatttcca tgacaagaca
     3601 gaaagcgccg tacagatatg caagcaagac ccggttatcg ataagacgaa cagactcagg
     3661 ctctgccggg tgttatgtga cggttgccgt tcgggggaag caggaattta ttattgctga
     3721 acttgattca gaaatccgga aaatcaggct gaaactgacg gactcctatg aagaaggggt
     3781 aaggctgtct tcgggcacgt ttacgcttcc ggccagattc tgccgggaaa tactgccgga
     3841 cgacgtcagg agtatcacca ttattctgga aaaaagcgac gatgagtggt ggtacgggag
     3901 ttactgatat gtggcagaac tccggacact gacgccttcg cgttgctcag ttgtccaacc
     3961 ccggaaacgg gaaaaagcaa gttttccccg ctcccggcgt ttctgcaata aaaagcaggg
     4021 gcatggaggt cgtcgtaacc agacaggaat gtggtattat cgcggcgggt gtctgagcct
     4081 ttctggttca ggcaagacgc aggtaccaga aatgcgaaga ccccactcgt taatccatta
     4141 actcgtgagg tctgcatgaa gtacttagca ctactgattg tagcctcttc tttgcagaga
     4201 ggtcaaagtt tatgacgaaa tatgccctta tcgggttgct cgccgtgtgt gccacggtat
     4261 tgtgtttttc actgatattc agggaacggt tatgtgaact gaatattcac aggggaaata
     4321 cagtggtgca ggtaactctg gcctacgaag cacggaagta agctgccggg cggggacgga
     4381 agtccccgct ttccggaagt gtgaggtatt tcaggggcag acacccgaca tgccagaaac
     4441 agccggtccc gcccggggcc ggcatccagg ttaaggcatt tcctgttttt cagtcatttc
     4501 atcatcaaaa tcacattaaa cggtcgtaat cagacatgat ttgtgcgcca acacagatca
     4561 tcatcacaac tttcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     4621 ctgtttgaga attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcatca
     4681 ggtacaaagc gagcataccg aaaaggtaat cctctgacgc ttgcagaacg tcagcaggca
     4741 tctctggcaa gaaaacgtgc cacacataaa gaactcagag tgttcattcc tgctgcgtta
     4801 aaagcgcaat tacaggagat gtgtgacgct gaaggtgtta ctcaggctga gatgattgct
     4861 gaactgataa agcagaagag tgctttcagc taactaaaat gtagcgacat cacattcttg
     4921 cctgtttgtg gtgtgcgtga tagattgctg atcgtttaag gaattttgtg gctggccacg
     4981 ccataaggtg gcagggaact ggttctgatg tggatgtaca ggagccagaa aagcgaaaac
     5041 cccgataatc ttctttaact ttggcgagtc agaaagatta ccggggctaa caagaaactg
     5101 catagaagct gttgctctat gcggggagta tagttatatg cccggaaaag ttcaagactt
     5161 ctttctgtgc tcactccttc tgtgcaacat aagtgcagga tggtgtgact gatcttcaac
     5221 aaacttatta ccgccaggta aagaacccga atccggtgtt tacaccccgt gaaggtgccg
     5281 gaacgctgaa gttttgcgaa aaactgatgg aaaaggcggt aggcttcacc tcccgttttg
     5341 atttcgccat tcatgtggca catgcccgtt cgcgtggtct gcgtcggcgc atgccaccgg
     5401 tactgcgtcg tcgggctatt gatgcgctgc tgcaggggct ctgttttcac tatgatccgc
     5461 tggccaaccg tgtccagtgc tccatcacca cgctggccat tgagtgtgga ctggcgacgg
     5521 agtctgatgc cggagtactc tccatcaccc gtgccacccg tgccctgacg tccctggctg
     5581 agctgggtct gattacctac cagacggaat atgacccgct tattggttgc aacattccga
     5641 ccgatatcac gttcacaccg gcgctgtttg ccgctctcga tgtgtctgag gtggcggtgg
     5701 cttccgcacg ccgcagccgt gtggaatggg aaaacagaca acgcaaaaag caggggctgg
     5761 ataccctggg catggatgaa ctgatagcga aagcctggcg ttttgttcgt gagcgtttcc
     5821 gcagttacca gacagagctt aagtcccggg gaataaagcg tgcccgtgcg cgtcgtgatg
     5881 cggacaggga acgtcaggat attatcaccc tggtgaaacg gcagctgacg cgcgaaatcg
     5941 cggaagggcg cttcactgcc agtcgtgagg cggtaaaacg tgaagtggag cgtcgtgtga
     6001 aggagcgcat gattctgtca cgtaaccgca attacagccg gctggccaca gcttccccct
     6061 gaaagtgacc tcttctgaat aatccggccc gaaccggagg cattcgcacg cctgaagcct
     6121 gccagcgaac aaaaaatcag caccacatac aaaaaacaac ctcactatcc accttctgga
     6181 gcatccggtt ccccctgttt ttaatacaaa atgcgcctca gcgacgggga attttgctta
     6241 tccacattta actgcaaggg acttccccat aaggttacaa ccgttcatgt cataaagcgc
     6301 cagccgccag tcttacaggg tgcaatgtat cttttaaaca cctgtttata tctcctttaa
     6361 actacttaat tacattcatt taaaaagaaa acctattcac tgcctgtcct gtggacagac
     6421 agatatgcac ctcccaccgc aagcggcggg ccccgaccgg agccacttta gttacaacac
     6481 acaaaaacaa cctccagaaa aaccccggtc cagcgcagaa ccgaaaccac aaagcccctc
     6541 cctcataact gaaaagcggc cccgccccgg cccaaagggc cggaacagag tcgcttttaa
     6601 ttatgaatgt tgtaactaca tcttcatcgc tgtcagtctt ctcgctggaa gttctcagta
     6661 cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa
     6721 accctcgtcg ggaccactcc gaccgcgcac agaagctctc tcatggctga aagcgggtat
     6781 ggtctggcag ggctggggat gggtaaggtg aaatctatca atcagtaccg gcttacgccg
     6841 ggcttcggcg gttttactcc tgtatcatat gaaacaacag agtgccgcct tccatgccgc
     6901 tgatgcggca tatcctggta acgatatctg aattgttata catgtgtata tacgtggtaa
     6961 tgacaaaaat aggacaagtt aaaaatttac aggcgatgca atgattcaaa cacgtaatca
     7021 atatctgcag tttatgctgg ttatgctggc tgcatggggc attagttggg gagccagatt
     7081 tgtcatggag caggccgttc tgctttatgg atcaggaaaa aactatttgt tcttcagtca
     7141 tggtactgtt ctgatgtacc tgctgtgtgt tttcctggta taccgccgtt ggatagctcc
     7201 gctaccggtc gttggtcagc tgcgcaacgt tggcgtaccg tggctggtcg gtgcgatggc
     7261 cgtggtgtat gtcggtgtat ttctgctcgg taaggcgctg gctctgcctg ctgagccatt
     7321 tatgacgaaa ctttttgccg ataagtccat acctgacgtg atcctgacgt tgctgaccat
     7381 ctttatcctt gccccgttga atgaggaaac gctgttccgg gggattatgc tgaacgtctt
     7441 ccgttcacgg tactgctgga cgatgtggct gggggcgctg ataacgtcgt tgttgttcgt
     7501 cgccgcgcac agccagtatc agaacctgct gacactggca gaactgttcc tggtggggtt
     7561 gattacatca gtggccagga tcagaagtgg tggcctgctg ctgccggtat tgctgcatat
     7621 ggaagcaacc acgctgggtt tactgtttgg ttgaaagtta tatttttatt aaacattgtg
     7681 cgttaaagcc tggtgtgttt ttttagtgga tgttatattt aaatataact tttatggagg
     7741 tgaagaatgc ataccacccg actgaagagg gttggcggct cagttatgct gaccgtccca
     7801 ccggcactgc tgaatgcgct gtctctgggc acagataatg aagttggcat ggtcattgat
     7861 aatggccggc tgattgttga gccgtacaga cgcccgcaat attcactggc tgagctactg
     7921 gcacagtgtg atccgaatgc tgaaatatca gctgaagaac gagaatggct ggatgcaccg
     7981 gcgactggtc aggaggaaat ctgacatgga aagaggggaa atctggcttg tctcgcttga
     8041 tcctaccgca ggtcatgagc agcagggaac gcggccggtg ctgattgtca caccggcggc
     8101 ctttaatcgc gtgacccgcc tgcctgttgt tgtgcccgta accagcggag gcaattttgc
     8161 ccgcactgcc ggctttgcgg tgtcgttgga tggtgttggc atacgtacca caggtgttgt
     8221 acgttgcgat caaccccgga caattgatat gaaagcacgg ggcggaaaac gactcgaacg
     8281 ggttccggag actatcatga acgaagttct tggccgcctg tccactattc tgacttgaac
     8341 atggggtttg aggggcaact ggatgaaaac gtacgattta gggcactaaa accgctgttg
     8401 tcccaccatt ctggtgattc ccaaacgtta tttggctaaa aagtagtttt gatgtggttt
     8461 attttccgaa ttccaagcgc agccctactt tcttgtgcgt atttgcgtgt tttgcgcagt
     8521 tttgaagttc cggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     8581 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
     8641 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
     8701 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
     8761 atggccatga atggcgttgg atgccgggca accgcccgca ttatgggcgt tggcctcaac
     8821 acgattttcc gccatttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
     8881 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggatacgtc ggggctaaat
     8941 cgcgccagcg ctggctgttt tacgcgtatg acaggctccg gaagacggtt gttgcgcacg
     9001 tattggggtc tgacgctcag tggaacgaaa actcacgtta agcaacgttt tctgcctctg
     9061 acgcctcttt taatggtctc agatgtcctt tggtcaccag ttctgccagc gtgaaggaat
     9121 aatggccgag catattgata tgtccgtggc aaagcgggga gaggcgtgcg atatcttcat
     9181 cattcagtgt ttcaccctgc gcccggagat gatccagggc tgcctgcata taaatagtgt
     9241 tccataacac gacggcgtta gtgaccagcc ccagtgcgcc cagttgatct tcctgaccgt
     9301 cggtatatcg ttttcttatc tcaccttttt gaccgtgaca gatggctctg gcaacggcat
     9361 ggcgactttc tccccgatta agctgggtca gaatgcgccg gcggtaatct tcatcatcaa
     9421 tataattaag cagatacagc gttttgttga tgcgccccac ttcaatgatt gcctgagtca
     9481 gtccggaagg acgttcactt ttcagcaatg aacggaccag cactgaagcc tgtactttgc
     9541 ccagcttcag ggagccagcg gtccggatca tttcgtccca ctgaaggact atttttggca
     9601 ctgttgcaaa gttagcgatg aggcagcctt ttgtcttatt caaaggcctt acatttcaaa
     9661 aactctgctt accaggcgca tttcgcccag gggatcacca taataaaatg ctgaggcctg
     9721 gcctttgcgt agtgcacgca tcacctcaat acctttgatg gtg
//
