LOCUS       NZ_CP147835            11970 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 130120D plasmid p2_130120D, complete
            sequence.
ACCESSION   NZ_CP147835
VERSION     NZ_CP147835.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40411204
            Assembly: GCF_037329525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of genetically modified Klebsiella pneumoniae
            strain 130120D
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Gong,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2024) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.5
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037329525.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:09:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,622
            CDSs (total)                      :: 5,502
            Genes (coding)                    :: 5,351
            CDSs (with protein)               :: 5,351
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 61 of 151
            Pseudo Genes (incomplete)         :: 96 of 151
            Pseudo Genes (internal stop)      :: 19 of 151
            Pseudo Genes (multiple problems)  :: 25 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="130120D"
                     /isolation_source="experimental"
                     /db_xref="taxon:573"
                     /plasmid="p2_130120D"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2021"
     gene            1296..3260
                     /locus_tag="WEO27_RS27910"
                     /old_locus_tag="WEO27_27910"
     CDS             1296..3260
                     /locus_tag="WEO27_RS27910"
                     /old_locus_tag="WEO27_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3260..3991
                     /gene="mobC"
                     /locus_tag="WEO27_RS27915"
                     /old_locus_tag="WEO27_27915"
     CDS             3260..3991
                     /gene="mobC"
                     /locus_tag="WEO27_RS27915"
                     /old_locus_tag="WEO27_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3998..4528
                     /locus_tag="WEO27_RS27920"
                     /old_locus_tag="WEO27_27920"
     CDS             3998..4528
                     /locus_tag="WEO27_RS27920"
                     /old_locus_tag="WEO27_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4555..4734)
                     /locus_tag="WEO27_RS27925"
                     /old_locus_tag="WEO27_27925"
     CDS             complement(4555..4734)
                     /locus_tag="WEO27_RS27925"
                     /old_locus_tag="WEO27_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4760..5188)
                     /locus_tag="WEO27_RS27930"
                     /old_locus_tag="WEO27_27930"
     CDS             complement(4760..5188)
                     /locus_tag="WEO27_RS27930"
                     /old_locus_tag="WEO27_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5352..5666)
                     /locus_tag="WEO27_RS27935"
                     /old_locus_tag="WEO27_27935"
     CDS             complement(5352..5666)
                     /locus_tag="WEO27_RS27935"
                     /old_locus_tag="WEO27_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5654..5932)
                     /locus_tag="WEO27_RS27940"
                     /old_locus_tag="WEO27_27940"
     CDS             complement(5654..5932)
                     /locus_tag="WEO27_RS27940"
                     /old_locus_tag="WEO27_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6107..6472)
                     /locus_tag="WEO27_RS27945"
                     /old_locus_tag="WEO27_27945"
     CDS             complement(6107..6472)
                     /locus_tag="WEO27_RS27945"
                     /old_locus_tag="WEO27_27945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(6469..6840)
                     /gene="tolA"
                     /locus_tag="WEO27_RS27950"
                     /old_locus_tag="WEO27_27950"
     CDS             complement(6469..6840)
                     /gene="tolA"
                     /locus_tag="WEO27_RS27950"
                     /old_locus_tag="WEO27_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7114..7359)
                     /locus_tag="WEO27_RS27955"
                     /old_locus_tag="WEO27_27955"
     CDS             complement(7114..7359)
                     /locus_tag="WEO27_RS27955"
                     /old_locus_tag="WEO27_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8004..9491
                     /gene="ltrA"
                     /locus_tag="WEO27_RS27960"
                     /old_locus_tag="WEO27_27960"
     CDS             8004..9491
                     /gene="ltrA"
                     /locus_tag="WEO27_RS27960"
                     /old_locus_tag="WEO27_27960"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9596..11281
                     /locus_tag="WEO27_RS27965"
                     /old_locus_tag="WEO27_27965"
     CDS             9596..11281
                     /locus_tag="WEO27_RS27965"
                     /old_locus_tag="WEO27_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            11291..11548
                     /locus_tag="WEO27_RS27970"
                     /old_locus_tag="WEO27_27970"
     CDS             11291..11548
                     /locus_tag="WEO27_RS27970"
                     /old_locus_tag="WEO27_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11633..11782
                     /locus_tag="WEO27_RS27975"
                     /old_locus_tag="WEO27_27975"
     CDS             11633..11782
                     /locus_tag="WEO27_RS27975"
                     /old_locus_tag="WEO27_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4596..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..494)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.984"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3261..3988
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta
       61 ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg gttggactca agtgaacagt
      121 taccggataa ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg
      181 agcgaacgac ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact
      241 tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg cagggtcgga acaggagagc
      301 gcaagaggga gcgacctacc ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc
      361 gtactgtcag attcatggtt gagcctcacg gctcccacag atgcaccgaa aaagcgtctg
      421 tttatgtgaa ctctggcagg agggcggagc ctatggaaaa acgccaccgg cgcggccctg
      481 ccgttttgcc tcacatgtta gtcccctgct tatccacgga atctgtgggt aactttgtat
      541 gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt gagcgaggag
      601 gcgcaatttc cccggcacct gcaagacggt caccggacgc gctgatgtgg gatattgtcc
      661 cctgattaag tctttctatt ccctgacgat caattatcag gataggtgaa ccatgtcggt
      721 ttttcgctga acattttacg ttaacctatc tctgttgctt ttttctcctt gcggagaaat
      781 gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa aagaccttgc ttacgctggc
      841 tggacgccac cgacgcacgg tcttttcccc tctttctggc tctgtccggc tggacgccgg
      901 ccacgccaga aattcacccg gcccccgtca ggctcacgcc tgaccaccca caaaaatcca
      961 cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttgcgg gtggtcagcc
     1021 gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc
     1081 gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg
     1141 catggggcgg aaagaggctg gcctccgcgg tgaaagctga acgcaatcac cacggacgtg
     1201 accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc tgcgcatcac
     1261 gccggtaaac ccgtcgtctg aaggagagag attgcatgtt taatacggat tcgcttgcct
     1321 ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc tggctgtacc
     1381 gggagtatta cagtctggcc ggtttttttc tggttatcgg cctgtttttc ggtctggctg
     1441 aactctgggc cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg cgggagtatg
     1501 ccgatcgcta taagcgcgat aataaccgga ctgtgccatt ttcggtgctg gcctcgctgg
     1561 attcacccgg gcggatggtc agggcacggc gacatgtctg gaactacgcg tactggctga
     1621 ccggcgctgc cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct
     1681 ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc agattattac ctgctgcagc
     1741 cggctaacct gttcgggata gcctggtgtg ccggagggct tgtcgccggc gtccttctgg
     1801 cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac gaggcggtga
     1861 acgcgaaact gaatcaggag agccggcgca gcagccagcg gaccggagaa atgacagacg
     1921 tccgccacct gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg
     1981 agcaggcccg gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc
     2041 tggtccctcg tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg
     2101 gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg
     2161 tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc
     2221 cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg
     2281 atctcgacca gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg
     2341 gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc
     2401 agttcccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg
     2461 ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg
     2521 tcctgcagac ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt
     2581 acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc
     2641 gctgcgcgca aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca
     2701 tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc
     2761 gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg
     2821 ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct
     2881 ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga
     2941 tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca
     3001 gcggcgacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac
     3061 acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca
     3121 gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa
     3181 cggacccgct ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg
     3241 attttgcggg gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca
     3301 ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc
     3361 cacggcggag ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt
     3421 cctggacagt ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg
     3481 tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga
     3541 gccgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca
     3601 ccggctggcc gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg
     3661 gtacctgcac cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga
     3721 cggcagaacg gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga
     3781 agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat
     3841 ctgccctgac cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat
     3901 cgtgaatcca cagaccggcg aggcccgcaa aacggtggag ctggaccggg agaggctgtt
     3961 cgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat
     4021 tgaggacgtg gaagacaaag acggcgagcg ctgcattatg ccaaccagcg agcatctgga
     4081 agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc
     4141 ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga
     4201 aaaagccacg tactttttca ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg
     4261 aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt
     4321 gcaggtggtt gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgaggtggt
     4381 cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga
     4441 atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca aacccgcccg
     4501 ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg catggccggt gctgttattc
     4561 ttccccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc
     4621 gcacatgtcg gccggggtat ccatgtccag ctcgttcagc ttttccagca acagcagcga
     4681 ctgatcctta atgaatttag ccatattgag ggcagttttt tcctgcttcg tcatgttctc
     4741 cattcccttc ggtaagttct cagctaaacc agccggacca gaagcgtttt ttcttctgtg
     4801 tcagttctgt tgtcaatctg tccacttcgg cctggagttt ccggcgctca tcatcgtgct
     4861 ggcggcgttc ttcttcccgg gatgttttat cttccagcat cagggtgaca gcctgccgga
     4921 gatcggccag ttccttttgc attgccagaa gctgttcctg tgggaaattt ttttgtgtat
     4981 tttcacggtg tgaaatgccg ggaatttgtt tctcagggat attcacattt ccgtatacac
     5041 ggatcagttc tgacacatcc acgacagggt tgtttttccc gtcacgtgac acggtaacct
     5101 ttccctgttt tacatgattg tatagcgttc ttctggtaat gcccgcggct tgtgctgcct
     5161 gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg attttatcgg gtgtgaaagg
     5221 tgtgtaattt ctcacggtaa atgtatcgct gaattttacc cgtttttccg ttcaggatgt
     5281 ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc
     5341 gtcatcggtt attagaggct gtctgccccc ggatacccgg attctttctg atgcctgacg
     5401 gtgacgatca caatctggtc catttcccgg tcgtggcggt agagcatcac atagccactg
     5461 tcgccaaacc cgatcaccag ttccttatat tccagcggca gaaacggcac cgggcggcca
     5521 atgtctggca atgttttcag ttgctggata gcccggacga tcacctcacc tccttttctg
     5581 gctgccagcc gatttttggt tttgagaaag tcctgaaggc gttgtaaatc gcgctgtgcc
     5641 agcgcggaaa tcgttacctg tggcatggcg gagcatcctg ctcgttctca gttccccagg
     5701 tacttatcca ggcttccgct tcttccgcag taaggtgcag acccgtttcc tgatagtgct
     5761 gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg tcgatgtatt
     5821 cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg gccagcgttt
     5881 tcagccggtc cttaagttct tcatcgagcc tgatggatgt tgctgatgac ataaaagccc
     5941 ctttgtagtc atatgtatta cacatgacta ctttaatccg tggcggcgct atttgccaga
     6001 aatacggctt ttgctgccca tttttcagct tcgcttccac taatcagggc tgctacaggc
     6061 tcactcatca tccaggacta ttgcagcctg cgggttaaag gacctgttac tggcggcaac
     6121 catcatcatt gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg tcatttttca
     6181 gactgaattc aaaatttata acctgtcgca ttgccgggtg ccctgcggga tatagccagt
     6241 gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc gacacgttgt
     6301 ccactcgtcc atcgtccaga atatcaaacg tggcggtcac ctgaccttcg atgcgttttg
     6361 ctgcggcctg taccgggtag gttggctgca gtgtggttac gggttttagt ggtatcgcat
     6421 gtaccggagt gaggaacatg gccgccagca ctgtcagagt aaaagctttc atggtctgaa
     6481 atccagaggg gcgtttttga atatttgcca ggtattttca tccggtgccg gcggaatttt
     6541 ggcgtgggta acggctgata ttgctgcttt acagagtttc ggatctcctc cttcagcaat
     6601 ggcgctgttc agagtgccat cacgttgtat ggatatccgc acggtgcaca tttttcccgc
     6661 ataggcctct ggttcaaaca tctgcatttc aacagcggtc ttgatggccg cagcgtagct
     6721 actgatttta gatgacttat ccagagtata aattttcggc ggctgggtgg tggaatgctt
     6781 acaggccgtc aggagtaagg ggctgcacaa tgccagcagg ataaagggta ttaaacgcat
     6841 cgggttgatg acctgtcagt aaaaaatgtt acatgtatat ttgtcactct ttacggccac
     6901 gttcatttac ataaacacgg cttatcgtat cgtttttaca ccaaacggcc cgccatgcag
     6961 ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc gcgtttccgg cggttttccg
     7021 ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac gccctcagac cgtctgagcg
     7081 gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg gctgccaggt
     7141 gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc gcctggcttc
     7201 acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc tgttgtttta ccgcctttgg
     7261 cagcgttttg ttccctttca ggcccatggc cagtgcctga gcgacaaagg cgtccgccag
     7321 gcgcttttct tcagcgggag tcagtttcag gtgcgtcata gtggcctccg ggcgtctcag
     7381 gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc agttagcttt cagatgtgtc
     7441 tataccatct gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg ttgccatgag
     7501 aaattttcgg gaaaagacgc gatctgctta caggtggctc cgggcgcttt tttgtgcctg
     7561 aagatgagaa ctaatctcat atattgacac ctgaaaacgg gaggagtaag gtaataatta
     7621 tactgtgtat atgtacagtg tgcgccggga gaccggttag agatcaaggg gtgaaagtcc
     7681 ccgaccattg aaggaccagc aatccacaag gtccccgagt catgcgttgc ataccgcgag
     7741 gtatggggcg aagcgttgac aggggtgttg acaggccagc cattgagcca cgaaatgtat
     7801 attaaattac cgggtgccga cgttgtactg ttaacggaag gcaacatcat agggtgcgac
     7861 actgcgagtg ccacatggac ccggcggggt ctgagaccct ggcatgtcaa tacgatctct
     7921 acgcgggaac cgggagatct cccctctgac catctgccag tgtcggagat ggcccgcacc
     7981 gggaagacga ggagtcatag ccggtgatgt acggagagga gaagtcggac tcgctcatag
     8041 tagcggcgaa gcaggcgaac aacccgaaag gagcggagtc agtggagcga aggagcgggg
     8101 ccaaggggaa cgcggaacag ccacacatgc gccggacaca gagccgggaa agcatgtcac
     8161 agaggctgtc acgcgtgcgg gaagctgcga agcagcggaa gaaagaacgg tttacagcat
     8221 tgttccacct gctgacagtc gaagcactgg aagccgcatt cctctccctg agcaggaaag
     8281 cggccgccgg agtggatggc atcaggtgga tggactacgc cggaaacatg aagaacaaca
     8341 taacagatct gcaccggagg ctacatcagg gcagctacag ggcgcagccc ggcaggcgtc
     8401 actacatccc aaaagcggat ggaaaacaac gcccgctcgg catcgcctcg ctggaggaca
     8461 agatcgtcca gtatgcgctg gtgaaaatcc tgaacgcagt ctatgaaaac gactttatgg
     8521 ggttctcata cgggttcaga cccgggcgaa gccagcacga tgcactggac gcactggcca
     8581 cagggctggt acgcactaac gtaaactggg tactggatgc cgacatcagt cagttcttcg
     8641 acagggtgag ccacgaatgg ctgatcaggt tcacagagca tcggatcggc gaccggaggg
     8701 taatcaggct catacgtaag tggctcacag ccgggacgtc ggaggagggt caatggcgag
     8761 caacggagga aggcacccca cagggtgcgg tcatctcacc gctgctggca aacatatacc
     8821 tccactacgt cttcgatctg tgggcgcatc agtggcgacg tcgctatgcc acaggcaatg
     8881 tggtaatggt cagatacgcc gatgacatcg tcatcgggtt cgacaaacga tacgatgccc
     8941 ggcgcttccg tatagccatg cagcgcagac tgagggagtt cggactcacg gttcacccgg
     9001 agaaaacccg tctgatggag ttcggccgct tcgctgccga aaaccgtgcc atcaggggaa
     9061 aaggcaaacc agaaacgttc aacttcctcg ggttcacgca catcagcggg aaagatcgca
     9121 acggcaggtt catgctgata cgaaagaccc gccgggatcg gatgacggca actctgaaag
     9181 ccatcaaaga cggtctgcga aggcgctggc attactcaat ccccgaacag ggaaaatggc
     9241 tcaggagagt ggttcaggga tacctgaact atcactcggt accgggcaac ttccccacca
     9301 tgcagaagtt caggacacac gtaacaaacc tctggcgccg ggcgctcagg cgcaggagcc
     9361 agaaggatga tacgacctgg acgaaagcaa acaaactggc agccgcatgg ctaccaaggg
     9421 ttcgggttct tcatccatgg cctgtggagc ggttcaccgc cagacacccg aggcaggagc
     9481 ccggtgcgta aatcgcgcac gccgggatct gtgcgggggg tatccggtaa cgggtatccc
     9541 taccgcgaca tgttggcttt aatttattgt tttaaatgtc aaagaggaaa acggtatgag
     9601 tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg gtcaggccag
     9661 tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg gtacggatcc
     9721 aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc ataactataa
     9781 tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta acagcggcaa
     9841 tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggcct tcggtctgcc
     9901 tgccctggct actccggggg ctgaaggact ggctttatca gtttccggcg atgcgttgtc
     9961 ggccgccgtt gctgatgtgc tggctgccct gaaggggccg tttaagtttg gtctgtgggg
    10021 gattgcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga aaatgatgtc
    10081 aaaaattatg acgtcattac cggccgatgc ggtgacggag actccggcaa gtactttacc
    10141 actggaccag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga aggatgagcg
    10201 gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg atgcgaaacc
    10261 gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag
    10321 cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg ctgaaaaagg
    10381 tgattcacgt ccggctggtt ttacagccgg tggtaactcc cgtgaggccg ttattcgttt
    10441 cccgaaagag accggacaga agccggttta tgtgtcggtg acagatgttc ttaccccggc
    10501 acaggtaaaa cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg acgccgctca
    10561 tccggaagag gggctgaaaa gagactatga taaagcgaaa gctgagctgg atgccgaaga
    10621 taaaaatatt gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc tccccggtgc
    10681 aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga
    10741 ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc aggttcgcta
    10801 tcttgataag gatattcaga atcagaatga gaaattaaaa gcggctcagg catctttaaa
    10861 cgcaatgaat gaatccttat ccagagataa ggctgcactt accggggcga tggagagccg
    10921 gaaacaaaag gagaaaaaag cgaaggatgc agaaaataag ttaaatgagg aaaagaacaa
    10981 gcctcgcaag gggactaaag actacggcca tgattatcat ccagtaccta aaacagaaga
    11041 cataaaggga cttggagagt taaagcgagg tgatcctaaa acacctaaac aaggtggtgg
    11101 tggtaagcgg gcccgatggt atggcgataa aaaacgtaaa atttatgaat gggattccca
    11161 gcacggtgag cttgaagggt atcgtgccag tgatggcgaa cacctaggcg catttgatcc
    11221 caaaacgggt aagcaggtta aagggccaga tccaaaacgg aacattaaaa aatatctttg
    11281 agaggtaaat atggggctta aattacgttt gacatggttt gataagaaaa ctgaagagtt
    11341 taaaggggaa gagtattcta aagactttgg tgacgatggt tctgtcattg aaagcttggg
    11401 aatgccttta aaggataata ttaataatgg tgagtttgat ttgaagaaag aatgggttcc
    11461 tttactacag ccatttttta aaaatcaaat aaaacctgat gtggataatt gttatatagc
    11521 ttttgattat cgggacggta actggtaaca aaaaattatc aaggatgatt tgaaatactg
    11581 acttccggtg ttcagggatg aatgccggag tctttaaata cagggaaagt atatgaaaaa
    11641 ggtaaagact atttttcttt ttatattgat tgttgctggc tttcttctgg ccgcatgtca
    11701 ggcaaattat atccgggatg ttcagggcgg aacggtggca ccatcgtcct cctctgaact
    11761 gacggggatc gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    11821 cgcgtaatct tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt
    11881 taagtcagtt ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa
    11941 atctgtcctt ccagtgtagc cggactttgg
//
